scholarly journals Emerging antimicrobial resistance, plasmid profile and pulsed-field gel electrophoresis pattern of the endonuclease-digested genomic DNA ofNeisseria gonorrhoeae

2000 ◽  
Vol 41 (3) ◽  
pp. 381 ◽  
Author(s):  
Kyungwon Lee ◽  
Jeong Won Shin ◽  
Jong Baeck Lim ◽  
Young Ah Kim ◽  
Dongeun Yong ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Plasmid Profile ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Resistance Plasmid ◽  
Ofneisseria Gonorrhoeae ◽  
Electrophoresis Pattern

scholarly journals Analysis of Legionella pneumophilaSerogroup 1 Isolates by Pulsed-Field Gel Electrophoresis

1999 ◽  
Vol 37 (1) ◽  
pp. 251-254 ◽  
Author(s):  
Thomas J. Marrie ◽  
Shaun Tyler ◽  
Gregory Bezanson ◽  
Charles Dendy ◽  
Wendy Johnson
Keyword(s):  
Nova Scotia ◽  
Legionella Pneumophila ◽  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Plasmid Profile ◽  
Pfge Pattern ◽  
Pulsed Field Gel Electrophoresis ◽  
Plasmid Content ◽  
Pulsed Field

At a hospital in Halifax, Nova Scotia, Canada, three strains of Legionella pneumophila were detectable based on plasmid content, while the isolates collected at another hospital in Halifax had no plasmids. Genomic DNA was digested withBssHII, SalI, and SpeI and subjected to pulsed-field gel electrophoresis (PFGE). We found no relationship between plasmid profile and PFGE pattern.

scholarly journals Molecular Analyses of Salmonellaenterica Isolates from Fish Feed Factories and Fish Feed Ingredients

2003 ◽  
Vol 69 (2) ◽  
pp. 1075-1081 ◽  
Author(s):  
Live L. Nesse ◽  
Kerstin Nordby ◽  
Even Heir ◽  
Bjarne Bergsjoe ◽  
Traute Vardund ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Profile Analysis ◽  
Plasmid Profile ◽  
Pulsed Field Gel Electrophoresis ◽  
Fish Feed ◽  
Molecular Analyses ◽  
Feed Ingredients ◽  
Pulsed Field ◽  
Salmonella Serovars

ABSTRACT Isolates of the most commonly observed salmonella serovars in Norwegian fish feed factories from 1998 to 2000 (Salmonella enterica serovar Agona, S. enterica serovar Montevideo, S. enterica serovar Senftenberg, and S. enterica serovar Kentucky) were studied by pulsed-field gel electrophoresis (PFGE) and plasmid profile analysis and compared to isolates of the same serovars from fish feed ingredients, humans, and other sources (a total of 112 isolates). Within each serovar, a variety of distinct PFGE types (with similarity levels less than 90%) were observed in the feed ingredients and other sources, while only two distinct types of each serovar were identified in the factories. The combined results of PFGE and plasmid analyses showed that each factory harbored only a few S. enterica clones. Some of these clones persisted for at least 3 years in the factories, indicating that there was long-lasting contamination probably due to inadequate decontamination procedures.

scholarly journals Molecular epidemiology and antimicrobial resistance of Salmonella isolates from broilers and pigs in Thailand

2019 ◽  
Vol 12 (8) ◽  
pp. 1311-1318 ◽  
Author(s):  
Dusadee Phongaran ◽  
Seri Khang-Air ◽  
Sunpetch Angkititrakul
Keyword(s):  
Antimicrobial Resistance ◽  
Gel Electrophoresis ◽  
Animal Species ◽  
Antimicrobial Agents ◽  
Genetic Relatedness ◽  
Pulsed Field Gel Electrophoresis ◽  
Resistance Pattern ◽  
Salmonella Spp ◽  
Pulsed Field ◽  
Amoxicillin Clavulanate

Aim: This study aimed to determine the prevalence and antimicrobial resistance pattern of Salmonella spp., and the genetic relatedness between isolates from broilers and pigs at slaughterhouses in Thailand. Materials and Methods: Fecal samples (604 broilers and 562 pigs) were collected from slaughterhouses from April to July 2018. Salmonella spp. were isolated and identified according to the ISO 6579:2002. Salmonella-positive isolates were identified using serotyping and challenged with nine antimicrobial agents: Amoxicillin/clavulanate (AMC, 30 μg), ampicillin (AMP, 10 μg), ceftazidime (30 μg), chloramphenicol (30 μg), ciprofloxacin (CIP, 5 μg), nalidixic acid (NAL, 30 μg), norfloxacin (10 μg), trimethoprim/sulfamethoxazole (SXT, 25 μg), and tetracycline (TET, 30 μg). Isolates of the predominant serovar Salmonella Typhimurium were examined for genetic relatedness using pulsed-field gel electrophoresis (PFGE). Results: Salmonella was detected in 18.05% of broiler isolates and 37.54% of pig isolates. The most common serovars were Kentucky, Give, and Typhimurium in broilers and Rissen, Typhimurium, and Weltevreden in pigs. Among broilers, isolates were most commonly resistant to antibiotics, NAL, AMP, TET, AMC, and CIP. Pig isolates most commonly exhibited antimicrobial resistance against AMP, TET, and SXT. Based on PFGE results among 52 S. Typhimurium isolates from broilers and pigs, a high genetic relatedness between broiler and pig isolates (85% similarity) in Cluster A and C from PFGE result was identified. Conclusion: The results revealed high cross-contamination between these two animal species across various provinces in Thailand. Keywords: antimicrobial resistance, broilers, pigs, pulsed-field gel electrophoresis, Salmonella spp.

scholarly journals Genotypic Characterization of Salmonella enteritidis Phage Types by Plasmid Analysis, Ribotyping, and Pulsed-Field Gel Electrophoresis

1998 ◽  
Vol 36 (8) ◽  
pp. 2314-2321 ◽  
Author(s):  
A. M. Ridley ◽  
E. J. Threlfall ◽  
B. Rowe
Keyword(s):  
Gel Electrophoresis ◽  
Salmonella Enteritidis ◽  
Profile Analysis ◽  
Phage Type ◽  
Plasmid Profile ◽  
Pulsed Field Gel Electrophoresis ◽  
Single Type ◽  
Pulsed Field ◽  
Phage Types ◽  
Reference Strains

Pulsed-field gel electrophoresis (PFGE) was used to resolveXbaI and SpeI macrorestriction fragments from 60 defined phage type (PT) reference strains of Salmonella enteritidis. The level of discrimination was compared to that afforded by plasmid profile analysis and ribotyping. Twenty-eight distinct XbaI pulsed-field profiles (PFPs) were observed, although a single type, PFP X1, predominated. Absence of the 57-kbspv-associated fragment was observed for three PT reference strains, and the profile was designated PFP X1A. The XbaI macrorestriction profiles of a further four PT reference strains were altered by the presence of plasmid-associated bands. Twenty-sixSpeI-generated PFPs (plus one subtype) were observed for the same strains. No SpeI fragment corresponding to the 38-MDa serovar-specific plasmid was detected. The distribution ofXbaI and SpeI profiles did not always correspond, producing a total of 32 combined PFPs for the 60 PT reference strains. This compared with a total of 18 different plasmid profiles and three PvuII ribotypes generated by the same strains. The results of this study indicate that PFGE may offer an improved level of discrimination over other genotypic typing methods for the epidemiological typing of S. enteritidis.

Antimicrobial Resistance of Salmonella enterica Isolates from Bulk Tank Milk and Milk Filters in the United States†

2013 ◽  
Vol 76 (1) ◽  
pp. 18-25 ◽  
Author(s):  
J. S. VAN KESSEL ◽  
J. SONNIER ◽  
S. ZHAO ◽  
J. S. KARNS
Keyword(s):  
Antimicrobial Resistance ◽  
Gel Electrophoresis ◽  
Salmonella Enterica ◽  
Pulsed Field Gel Electrophoresis ◽  
Class I ◽  
Bulk Tank Milk ◽  
Pulsed Field ◽  
Extended Spectrum ◽  
Class I Integrons ◽  
Bulk Tank

Salmonella isolates were recovered from bulk tank milk as part of the National Animal Health Monitoring System (NAHMS) Dairy 2002 and 2007 surveys. In-line milk filters were also tested in the 2007 survey. The objective of this study was to determine the prevalence of antimicrobial resistance among Salmonella enterica isolates from bulk milk and milk filters in the NAHMS Dairy 2002 and 2007 surveys and to further characterize resistant isolates. Susceptibilities to 15 antibiotics were determined for 176 Salmonella isolates of 26 serotypes using an automated antimicrobial susceptibility system. Resistant isolates were screened by PCR for the presence of the extended-spectrum β-lactamase (blaCMY) gene and class I integrons and further characterized by pulsed-field gel electrophoresis. Thirty isolates (17.0%) representing six S. enterica serotypes exhibited resistance to at least one antimicrobial agent (serotypes Newport [14 of 14 isolates exhibited resistance], Dublin [7 of 7], Typhimurium [3 of 5], Kentucky [4 of 22], Anatum [1 of 13], and Infantis [1 of 2]). Twenty isolates (11.4%), including all 14 Newport, 3 Dublin, 2 Typhimurium, and 1 Infantis isolate, displayed the typical multidrug-resistant, blaCMY-positive (MDR-AmpC) phenotype which included resistance to ampicillin, chloramphenicol, streptomycin, sulfonamide, and tetracycline, plus resistance to amoxicillin–clavulanic acid and extended-spectrum cephalosporins. Five of the MDR-AmpC isolates carried class I integrons (2.8%). Two-enzyme (XbaI and BlnI) pulsed-field gel electrophoresis discerned clades within serotypes and, together with the resistance profiles, identified strains that appeared to have persisted temporally and geographically. These results suggest that there is a low but appreciable risk of infection with MDR Salmonella from consumption of nonpasteurized milk and dairy products.

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