scholarly journals A Case of Multiple Myeloma with Unusual Serum Protein Electrophoresis

Pulse ◽  
2016 ◽  
Vol 8 (1) ◽  
pp. 77-80
Author(s):  
Waheeda Nargis ◽  
Mohammad Ibrahim
Keyword(s):  
Multiple Myeloma ◽  
Gel Electrophoresis ◽  
Monoclonal Gammopathy ◽  
Protein Electrophoresis ◽  
Immunoglobulin M ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Monoclonal Immunoglobulin ◽  
Multiple Myeloma Patient ◽  
M Spike

Monoclonal gammopathy is a group of B-cell disorders resulting in the secretion of a specific and unique monoclonal immunoglobulin (M-component); best detecting with high resolution agarose gel electrophoresis. An M-protein is usually visible as a localized band on agarose gel electrophoretic peak in the beta, gamma, or rarely in the alpha-2globulin region of the densitometer tracing. Here, we presented a multiple myeloma patient with IgA kappa paraprotein showing an M spike in the alpha-2 globulin region in agarose gel electrophoresis.Pulse Vol.8 January-December 2015 p.77-80

scholarly journals Multiple Myeloma with Suspected Non-Secretory Type

2020 ◽  
Vol 27 (1) ◽  
Author(s):  
Annisa Ginar Indrarsi ◽  
Usi Sukorini
Keyword(s):  
Multiple Myeloma ◽  
Bone Marrow ◽  
Monoclonal Gammopathy ◽  
Plasma Cells ◽  
Immunoglobulin A ◽  
Protein Electrophoresis ◽  
Palpebral Conjunctiva ◽  
General Weakness ◽  
M Spike ◽  
G Ratio

Multiple Myeloma (MM) is a hematological malignancy characterized by clonal plasma cell in bone marrow that produceabnormal globulin, which resulted in monoclonal gammopathy. Multiple Myeloma Non-Secretory (MMNS) is a very rareform of multiple myeloma with monoclonal plasmocytic proliferation in bone marrow supported by clinical manifestationand radiological findings. However, plasma cells fail to secrete immunoglobulin. A 44-year-old female came to SardjitoGeneral Hospital with main complaints of weakness and back pain. General weakness and pale palpebral conjunctiva were6 observed (+/+), liver and spleen were not palpable. Blood test results were as follows: Hb 3.0 g/dL, RBC 1.07 x 10 / μL, WBC3 3 562 x 10 /μL, PLT 114 x 10 /μL, A/G ratio 1.07, BUN 51.5 mg/dL, creatinine 4.62 mg/dL, and calcium 3.1 mmol/L. Skeletalsurvey suggested a multiple osteolytic. Protein electrophoresis revealed hypogammaglobulinemia with no M-spike. Therewere 66% of plasma cells in bone marrow. Patient was diagnosed by MMNS. Diagnosis MMNS can be established if clonalplasmacytes is accompanied with renal insufficiency and hypercalcemia. However, monoclonal gammopathy was not foundin serum protein electrophoresis. A case reported of 44-year-old female diagnosed as MMNS with 'punched out' multipleosteolytic, increased plasma cells in bone marrow without evidence of paraprotein in circulation proved by low A/G ratio andnegative M-spike.

Feline Lymphoplasmacytic Stomatitis Associated with Monoclonal Gammopathy and Bence-Jones Proteinuria

1994 ◽  
Vol 11 (1) ◽  
pp. 25-27 ◽  
Author(s):  
Kenneth F. Lyon
Keyword(s):  
Multiple Myeloma ◽  
Oral Cavity ◽  
Serum Protein ◽  
Monoclonal Gammopathy ◽  
Alkylating Agent ◽  
Protein Electrophoresis ◽  
M Protein ◽  
Monoclonal Immunoglobulin ◽  
Urine Protein ◽  
Bence Jones Proteinuria

Lymphoplasmacytic stomatitis and gingivitis was diagnosed in an 8-year old female domestic shorthair. The cat had evidence of severe generalized inflammation of the oral cavity. Biopsy samples were evaluated and displayed a lichenoid, interface stomatitis which was predominantly lymphoplasmacytic. Serum protein electrophoresis confirmed a monoclonal gammopathy. Urine protein electrophoresis confirmed Bence-Jones proteinuria. Protein electrophoresis was used to diagnose monoclonal gammopathy (the production of a monoclonal immunoglobulin, or paraprotein, which is associated with a characteristic “M” protein spike on serum electrophoresis). Diseases associated with monoclonal gammopathy are similar in the dog and cat. Alkylating agent chemotherapy is used to rapidly reduce paraprotein concentrations in multiple myeloma. Multiple myeloma is the most common disorder associated with monoclonal gammopathy. This condition is less common in the cat, compared to the dog. This report examines the diagnosis and treatment of multiple myeloma in a cat presenting with severe stomatitis.

scholarly journals Sodium dodecyl sulfate-agarose gel electrophoresis of urinary proteins: application to multiple myeloma

1998 ◽  
Vol 44 (6) ◽  
pp. 1191-1197 ◽  
Author(s):  
Thierry Le Bricon ◽  
Danielle Erlich ◽  
Djaouida Bengoufa ◽  
Michelle Dussaucy ◽  
Jean-Pierre Garnier ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Sodium Dodecyl Sulfate ◽  
Renal Dysfunction ◽  
Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
High Sensitivity ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Urinary Proteins ◽  
Dodecyl Sulfate

Abstract We evaluated a new sodium dodecyl sulfate-agarose gel electrophoresis (SDS-AGE) for urinary protein analysis in patients with multiple myeloma (MM; n = 47; ages, 62 ± 2 years, mean ± SE). Abnormal proteinuria (mean = 1872 ± 360 mg/24 h) was present in 95% of the samples; 75% of the patients had some sign of renal dysfunction (glomerular and/or tubular) according to their SDS-AGE pattern. A band suggesting Bence Jones proteinuria (BJP) was detected in 40 vs 33 specimens by routine AGE. Immunofixation identified BJP in 38 patients; the calculated sensitivity of SDS-AGE for BJP was 97%. Excellent correlation (P <0.0001) was obtained with routine AGE (r = 0.994) and immunonephelometry (r = 0.963) for light chain quantification. SDS-AGE allows easy evaluation of renal dysfunction and shows high sensitivity for BJP detection. In a specialized laboratory, it is useful for following the progress of MM patients through the semiquantification of BJP.

scholarly journals Comparison of Bromcresol Green and Agarose Protein Electrophoresis for Quantitation of Serum Albumin in Multiple Myeloma

2007 ◽  
Vol 53 (6) ◽  
pp. 1099-1103 ◽  
Author(s):  
Christine LH Snozek ◽  
Amy K Saenger ◽  
Philip R Greipp ◽  
Sandra C Bryant ◽  
Robert A Kyle ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Serum Albumin ◽  
Staging System ◽  
Protein Electrophoresis ◽  
Modular System ◽  
Agarose Gel ◽  
Myeloma Patient ◽  
Clinically Significant ◽  
M Spike ◽  
Bromcresol Green

Abstract Background: The International Staging System for multiple myeloma has increased the importance of accurate measurement of serum albumin. Two common albumin assays, bromcresol green (BCG) and agarose gel protein electrophoresis (PEL), frequently yield discordant results, creating confusion regarding which assay is superior for use in myeloma. Methods: We measured albumin by BCG on a Roche Modular system, by PEL with a Helena SPIFE SPE Vis agarose gel, and by immunonephelometry performed on a Dade Behring BNII nephelometer. BCG and PEL were used to measure albumin in 5777 patient samples, and all 3 methods were used in an additional 252 samples. The clinical impact was assessed on 698 myeloma patient samples. Results: For sera with zero/low monoclonal immunoglobulin protein (M)-spike (0 to <15 g/L), results for both BCG and PEL correlated well to nephelometry, although median PEL results were 8 g/L lower than corresponding BCG measurements. Correlation between PEL and nephelometry or BCG diminished with increasing M-spike, with PEL eventually overestimating albumin compared with both other assays. IgG and IgA M-spikes showed significantly different effects on albumin discordance. For 35% of myeloma patients, discrepancy between BCG and PEL had a potentially clinically significant effect on staging, but no difference in group survival was found. Conclusions: Both BCG and PEL correlate well to nephelometry in sera with zero/low M-spikes. In the presence of larger M-spikes, PEL correlates poorly to nephelometry or BCG, whereas BCG compares well with nephelometry regardless of M-spike. Thus, albumin measurement can be performed reliably in myeloma patient sera by use of inexpensive, automated BCG assays.

scholarly journals Serum monoclonal immunoglobulin bands in undifferentiated lymphomas of Burkitt and non-Burkitt types

Blood ◽  
1983 ◽  
Vol 61 (4) ◽  
pp. 726-731
Author(s):  
I Magrath ◽  
D Benjamin ◽  
N Papadopoulos
Keyword(s):  
High Resolution ◽  
Tumor Markers ◽  
Light Chain ◽  
Gel Electrophoresis ◽  
Lymphoblastic Lymphoma ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Monoclonal Immunoglobulin ◽  
Potential Value

Using an improved electroimmunofixation technique that combines the sensitivity of high resolution agarose gel electrophoresis with the specificity of immunoprecipitation, we have demonstrated monoclonal immunoglobulin bands in the serum of patients with undifferentiated lymphomas of Burkitt and non-Burkitt types. Monoclonal bands were detected in the serum of 12 of 21 patients with extensive tumor, and 1 of 10 patients with minimal tumor. All of the bands were identified as IgM of a single light chain class. Such bands were not detected in the serum of patients with lymphoblastic lymphoma (7) or African Burkitt's lymphoma (6). There was disappearance of the bands after therapy and reappearance at relapse. These findings, coupled with previously reported in vitro information, indicate that undifferentiated lymphoma cells secrete immunoglobulin of IgM isotype. Therefore, such monoclonal bands may be of potential value as tumor markers.

scholarly journals Serum monoclonal immunoglobulin bands in undifferentiated lymphomas of Burkitt and non-Burkitt types

Blood ◽  
1983 ◽  
Vol 61 (4) ◽  
pp. 726-731 ◽  
Author(s):  
I Magrath ◽  
D Benjamin ◽  
N Papadopoulos
Keyword(s):  
High Resolution ◽  
Tumor Markers ◽  
Light Chain ◽  
Gel Electrophoresis ◽  
Lymphoblastic Lymphoma ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Monoclonal Immunoglobulin ◽  
Potential Value

Abstract Using an improved electroimmunofixation technique that combines the sensitivity of high resolution agarose gel electrophoresis with the specificity of immunoprecipitation, we have demonstrated monoclonal immunoglobulin bands in the serum of patients with undifferentiated lymphomas of Burkitt and non-Burkitt types. Monoclonal bands were detected in the serum of 12 of 21 patients with extensive tumor, and 1 of 10 patients with minimal tumor. All of the bands were identified as IgM of a single light chain class. Such bands were not detected in the serum of patients with lymphoblastic lymphoma (7) or African Burkitt's lymphoma (6). There was disappearance of the bands after therapy and reappearance at relapse. These findings, coupled with previously reported in vitro information, indicate that undifferentiated lymphoma cells secrete immunoglobulin of IgM isotype. Therefore, such monoclonal bands may be of potential value as tumor markers.

scholarly journals Molecular and Biochemical Methods Used for the Identification of Globodera Species in Slovenia (from 6th Conference of European Foundation for Plant Pathology, Prague, Czech Republic, 8–14 September 2002)

2011 ◽  
Vol 39 (No. 4) ◽  
pp. 151-153
Author(s):  
S. Širca ◽  
G. Urek ◽  
V. Meglič
Keyword(s):  
Plant Pathology ◽  
Czech Republic ◽  
Gel Electrophoresis ◽  
Morphological Characteristics ◽  
Protein Electrophoresis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Biochemical Methods ◽  
Pcr Rflp ◽  
European Foundation

Globodera species identification is based on specific morphological characteristics which are included in the majority of keys used for Globodera identification. To confirm and make more precise the morphometrical method, other methods have been developed. At Agricultural Institute of Slovenia, molecular and biochemical methods are applied. With the use of PCR-RFLP method we try to distinguish between G. rostochiensis, G. pallida and G. tabacum. Patterns of nematode DNA digested with restriction endonucleases and subjected to agarose gel electrophoresis are analysed. Differences in DNA sequence result in the number and size of fragments produced (RFLPs). For the differentiation of all three nematodes we have introduced a method for protein electrophoresis. Samples are compared after being separated by polyacrilamide slab gel electrophoresis. Proteins are visualised after silver staining.        

Abrnomal lipoprotein patterns in human serum as determined by agarose gel electrophoresis.

1979 ◽  
Vol 25 (11) ◽  
pp. 1885-1887 ◽  
Author(s):  
N M Papadopoulos
Keyword(s):  
Multiple Myeloma ◽  
Human Serum ◽  
Gel Electrophoresis ◽  
The Other ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Tangier Disease ◽  
Serum Lipoproteins ◽  
Electrophoretic Patterns ◽  
Electrophoretic Technique

Abstract Two groups of abnormal electrophoretic patterns of serum lipoproteins are reported here. One demonstrates a deficiency or absence of lipoprotein fractions, which is characteristic of patients with abeta-lipoproteinemia, hypo-beta-lipoproteinemia, or Tangier disease. The other shows the presence of extra lipoprotein fractions, as found in cholestasis and multiple myeloma. These patterns, together with those of hyperlipoproteinemia phenotypes previously reported (Clin. Chem. 24:227, 1978), form a reference record and a basis for the detection and evaluation of lipoprotein abnormalities in normal and dyslipoproteinemic subjects, as determined by a sensitive, accurate, rapid, and inexpensive electrophoretic technique.

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