8. Confirmation Of a Quantitative PCR Assay, Incorporating sample Inhibition Detection using Internal Standard Controls, for Analysis of Airborne Aspergillus Fumigatus Conidia Collected on Filters

2004 ◽  
Author(s):  
J. McDevitt ◽  
P. Lees ◽  
K. Schwab ◽  
W. Merz
Keyword(s):  
Aspergillus Fumigatus ◽  
Quantitative Pcr ◽  
Internal Standard ◽  
Pcr Assay ◽  
Sample Inhibition ◽  
Quantitative Pcr Assay

Identification and quantification of Fusarium oxysporum in planta and soil by means of an improved specific and quantitative PCR assay

2010 ◽  
Vol 46 (3) ◽  
pp. 372-382 ◽  
Author(s):  
Daniel Jiménez-Fernández ◽  
Miguel Montes-Borrego ◽  
Juan A. Navas-Cortés ◽  
Rafael M. Jiménez-Díaz ◽  
Blanca B. Landa
Keyword(s):  
Fusarium Oxysporum ◽  
Quantitative Pcr ◽  
In Planta ◽  
Pcr Assay ◽  
Quantitative Pcr Assay ◽  
Identification And Quantification

scholarly journals Culture-Independent Detection of Nontuberculous Mycobacteria in Clinical Respiratory Samples

2016 ◽  
Vol 54 (9) ◽  
pp. 2395-2398 ◽  
Author(s):  
Gianny P. Scoleri ◽  
Jocelyn M. Choo ◽  
Lex E. X. Leong ◽  
Thomas R. Goddard ◽  
Lisa Shephard ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Nontuberculous Mycobacteria ◽  
Direct Detection ◽  
Clinical Samples ◽  
Pcr Assay ◽  
Content Type ◽  
Culture Independent ◽  
Quantitative Pcr Assay ◽  
Single Reaction

Culture-based detection of nontuberculousMycobacteria(NTM) in respiratory samples is time consuming and can be subject to overgrowth by nonmycobacterial bacteria. We describe a single-reaction TaqMan quantitative PCR assay for the direct detection of NTM species in clinical samples that is specific, sensitive, and robust.

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