174. Dermal Exposure Model for JP-8 Jet Fuel Exposure Using the Tape-Stripping Method

2003 ◽  
Author(s):  
Y. Chao ◽  
L. Nylander-French
Keyword(s):  
Dermal Exposure ◽  
Jet Fuel ◽  
Exposure Model ◽  
Tape Stripping ◽  
Stripping Method

Validation of the dermal exposure model in ECETOC TRA

2017 ◽  
Vol 61 (7) ◽  
pp. 854-871 ◽  
Author(s):  
Hans Marquart ◽  
Remy Franken ◽  
Henk Goede ◽  
Wouter Fransman ◽  
Jody Schinkel
Keyword(s):  
Dermal Exposure ◽  
Exposure Model

scholarly journals Dermal Advanced REACH Tool (dART)—Development of a Dermal Exposure Model for Low-Volatile Liquids

2019 ◽  
Vol 63 (6) ◽  
pp. 624-636 ◽  
Author(s):  
Henk A Goede ◽  
Kevin McNally ◽  
Jean-Philippe Gorce ◽  
Hans Marquart ◽  
Nick D Warren ◽  
...  
Keyword(s):  
Mechanistic Model ◽  
Predictive Ability ◽  
Field Studies ◽  
Dermal Exposure ◽  
Exposure Model ◽  
Sufficient Evidence ◽  
Software Platform ◽  
Related Factors ◽  
Direct Emission ◽  
Volatile Liquids

Abstract This article describes the development of a mechanistic model for underpinning the dermal Advanced REACH Tool (dART), an extension of the existing ART model and its software platform. It was developed for hand exposure to low volatile liquids (vapour pressure ≤ 10 Pa at 20°C) including solids-in-liquid products. The model is based on an existing conceptual dermal source-receptor model that has been integrated into the ART framework. A structured taxonomy of workplace activities referred to as activity classes are adopted from ART.  Three key processes involved in mass transport associated with dermal exposure are applied, i.e. deposition, direct emission and contact, and transfer. For deposition, the model adopts all the relevant modifying factors (MFs) applied in ART. In terms of direct emission and contact (e.g. splashes) and transfer (e.g. hand-surface contacts), the model defines independent principal MFs, i.e. substance-related factors, activity-related factors, localized- and dispersion control and exposed surface area of the hands. To address event-based exposures as much as possible, the model includes crucial events during an activity (e.g. hand immersions) and translates objective information on tools and equipment (manual or automated) to probable events (e.g. splashes) and worker behaviours (e.g. surface contacts). Based on an extensive review of peer-reviewed literature and unpublished field studies, multipliers were assigned to each determinant and provide an approximated (dimensionless) numerical value. In the absence of (sufficient) evidence, multipliers were assigned to determinants based on assumptions made during discussions by experts in the consortium. A worked example is presented to illustrate the calculation of hand exposure for a specific scenario. The dART model is not yet implemented in the ART software platform, and a robust validation of the model is necessary to determine its predictive ability. With advancing knowledge on dermal exposure and its determinants, this model will require periodic updates and refinements, in addition to further expansion of the applicability domain of the model.

scholarly journals Bioequivalence of Topical Clotrimazole Formulations: An Improved Tape Stripping Method

2011 ◽  
Vol 14 (3) ◽  
pp. 347 ◽  
Author(s):  
Natalie Rae Parfitt ◽  
Michael Frederick Skinner ◽  
Charles Bon ◽  
Isadore Kanfer
Keyword(s):  
Reference Product ◽  
Transepidermal Water Loss ◽  
Hplc Method ◽  
Tape Stripping ◽  
Compelling Evidence ◽  
Application Time ◽  
Stripping Method ◽  
Bioequivalence Assessment ◽  
Tape Strip

Purpose: Investigations were carried out to assess the use of tape stripping (TS) for the determination of bioequivalence of topical products containing 1% clotrimazole. Methods: The study design involved the establishment of an appropriate application time, which was determined by conducting a dose duration study. Subsequently, two bioequivalence studies were conducted: i) using the brand (Canesten Topical - 1% clotrimazole cream) as both the test and the reference product and ii) comparing Canesten cream with a gel product containing the same concentration of clotrimazole (1%). Each tape strip was individually analyzed for clotrimazole content using an HPLC method and Transepidermal Water Loss (TEWL) measurements were used to normalize the stratum corneum thicknesses between subjects. Results: The results of the TS investigations showed that, if the study is sufficiently powered, tape stripping may be used to determine bioequivalence according to the conventional bioequivalence limits of 0.8–1.25, as well as detect formulation differences between different clotrimazole products. Conclusions: The data from this study provided compelling evidence that tape stripping has the necessary attributes and potential to be used as a tool for the bioequivalence assessment of topical clotrimazole and/or other topical formulations, thereby circumventing the need to undertake expensive and time-consuming clinical trials for such products. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

Tape Stripping Method and Stratum Corneum

2004 ◽  
pp. 531-547 ◽  
Author(s):  
Hongbo Zhai ◽  
Howard Maibach ◽  
Myeong Jun Choi
Keyword(s):  
Stratum Corneum ◽  
Tape Stripping ◽  
Stripping Method

scholarly journals Evaluation of the Stratum Corneum Exfoliated by Tape Stripping Method (No. 1)

1997 ◽  
Vol 32 (1) ◽  
pp. 33-42 ◽  
Author(s):  
Masayuki Matsumoto ◽  
Shoji Hayashi ◽  
Seiichi Arai
Keyword(s):  
Stratum Corneum ◽  
Tape Stripping ◽  
Stripping Method

scholarly journals Tape stripping method is useful for the quantification of antimicrobial peptides on the human skin surface including the stratum corneum

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shigeyuki Ono ◽  
Nobuhiko Eda ◽  
Takuya Mori ◽  
Atsuko Otsuka ◽  
Nobuhiro Nakamura ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Stratum Corneum ◽  
Human Skin ◽  
Significant Positive Correlation ◽  
Skin Surface ◽  
Tape Stripping ◽  
Established Method ◽  
Positive Correlation ◽  
Physiological Importance ◽  
Stripping Method

Abstract Antimicrobial peptides (AMPs) play an important role in innate immunity in human skin. It is known that AMPs mainly function in the stratum corneum. Therefore, AMP concentrations in the stratum corneum need to be precisely measured to clarify functional and physiological importance of AMPs in cutaneous defence. Tape stripping (TS) is a well-established method by which components in the stratum corneum can be collected. However, the usefulness of the TS method for measuring AMP concentration in human skin remains unclear. Therefore, we compared it with another popular method, skin rinsing, which had been established as a method for measuring AMP concentration in human skin. When investigated on healthy medial forearm using RNase 7, which is one of the typical AMPs, as an index, there was a significant positive correlation between RNase 7 concentrations measured by the TS method at adjacent forearm sites, demonstrating the reproducibility of the TS method. Next, a significant positive correlation was detected in RNase 7 concentrations measured using the TS and the skin rinsing method, indicating that the TS method is comparable to the skin rinsing method. Thus, we speculate that the TS method is useful for measuring AMP concentration in human skin.

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