scholarly journals Investigation of Differences in the Cultivation of Nannochloropsis and Chlorella species by Fourier-transform Infrared Spectroscopy

2018 ◽  
Vol 62 (4) ◽  
Author(s):  
Bernadett Kiss ◽  
Szilveszter Gergely ◽  
András Salgó ◽  
Áron Németh

The increasing use of energy in the world is leading to the exhaustion of fossil fuels, so novel alternative solutions have to be found to meet our needs. One solution is renewable raw materials extracted from algae. The use of microalgae is widespread, in addition to energy formation, their biomass can also be utilized as food and other valuable components of them, e.g. amino acids, vitamins and minerals can be used in drugs and cosmetics. Due to their boundless diversity and components, they have become the focus of an ever-increasing number of research areas. Different processes can induce changes in their nutritional content, so optimizing the conditions used during their cultivation is important to produce the desired product. In our study different isolates of microalgae, namely Nannochloropsis sp. and Chlorella vulgaris, were studied using Fourier-transform infrared (FT-IR) spectroscopic analysis. Variations in the spectra of a given species were studied under different cultivation conditions.

2019 ◽  
Vol 73 (7) ◽  
pp. 767-773
Author(s):  
Ryan C. Ogliore ◽  
Cosette Dwyer ◽  
Michael J. Krawczynski ◽  
Hélène Couvy ◽  
Max Eisele ◽  
...  

We report an infrared (IR) spectroscopic technique to detect quartz grains with large isotope anomalies. We synthesized isotopically doped quartz and used Fourier transform infrared spectroscopy (FT-IR) in two different instruments: a traditional far-field instrument and a neaSpec nanoFT-IR, to quantify the shift in the peak of the Si–O stretch near 780 cm−1 as a function of isotope composition, and the uncertainty in this shift. From these measurements, we estimated the minimum detectable isotope anomaly using FT-IR. The described technique can be used to nondestructively detect very small (30 nm) presolar grains. In particular, supernova grains, which can have very large isotope anomalies, are detectable by this method.


2007 ◽  
Vol 61 (7) ◽  
pp. 755-764 ◽  
Author(s):  
Christian Vogel ◽  
Shigeaki Morita ◽  
Harumi Sato ◽  
Isao Noda ◽  
Yukihiro Ozaki ◽  
...  

The thermal degradation behavior of poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate- co-3-hydroxyhexanoate) (P(HB- co-HHx), HHx = 12 mol%) has been studied under different environmental conditions by thermogravimetric analysis (TGA) Fourier transform infrared (FT-IR) spectroscopy. It is reported that at higher temperature (>400 °C) carbon dioxide and propene are formed from the decomposition product crotonic acid in a nitrogen atmosphere, whereas in an oxygen atmosphere propene oxidizes in a further step to carbon dioxide, carbon monoxide and hydrogen. It was also found that PHB and P(HB- co-HHx) have a similar thermal degradation mechanism. The analysis of the FT-IR-spectroscopic data was performed with 2D and perturbation-correlation moving-window 2D (PCMW2D) correlation spectroscopy.


The Analyst ◽  
2017 ◽  
Vol 142 (13) ◽  
pp. 2475-2483 ◽  
Author(s):  
H. Shinzawa ◽  
B. Turner ◽  
J. Mizukado ◽  
S. G. Kazarian

FT-IR spectra of a HEK cell were analyzed with 2D disrelation mapping to reveal molecular states of water and protein hydration.


2003 ◽  
Vol 17 (2-3) ◽  
pp. 315-321 ◽  
Author(s):  
Kazuyuki Yano ◽  
Yasushi Sakamoto ◽  
Narumi Hirosawa ◽  
Shouko Tonooka ◽  
Hiroo Katayama ◽  
...  

Glycogen levels in human lung and colorectal cancerous tissues were measured by the Fourier transform (FT-IR) spectroscopic method. Reliability of this method was confirmed by chemical analyses of the same tissues used for the FT-IR spectroscopic measurements, suggesting that this spectroscopic method has a high specificity and sensitivity in discriminating human cancerous tissues from noncancerous tissues. The glycogen levels in the tissues were compared with the clinical, histological and histopathological factors of the cancer, demonstrating that glycogen is a critical factor in understanding the biological nature of neoplastic diseases. Furthermore, direct measurement of a very small amount of tissue by a FT-IR microscope suggested that it could be used as a diagnostic instrument for various tissue samples obtained via a fine needle biopsy procedure. The progressive alterations in rat mammary gland tumors were investigated by a near-infrared (NIR) spectrometer with a fiber optic probe. A lipid band due to the first overtone ofn-alkane was used to quantitatively evaluate malignant changes in the tumors. NIR spectroscopy may offer the potential for non‒invasive,in vivodiagnosis of human cancers.


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