scholarly journals Pathological and molecular detection of diseases of cattle at slaughter

2019 ◽  
Author(s):  
A. A. Jahan ◽  
T. Ruba ◽  
T. T. Mumu ◽  
M. S. Rana ◽  
M. Islam ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Histopathological Examination ◽  
Zoonotic Diseases ◽  
Gram Stain ◽  
Chain Reaction ◽  
Tissue Sections ◽  
Petechial Hemorrhage ◽  
Pcr Technology ◽  
Hematoxylin And Eosin ◽  
Polymerase Chain

Background: This study was carried out to identify important zoonotic diseases of beef cattle at slaughter in Bangladesh. Methods: A total of 20 slaughtered cattle of both sexes, different ages and breeds were taken under investigation from Nilphamari, Rajshahi and Mymensingh district, Bangladesh during July to October, 2017. Detailed ante-mortem and post-slaughter lesions were inspected and the tissues from the lungs, liver, kidney and spleen were collected aseptically for histopathological examination. The tissue sections were processed and stained with hematoxylin and eosin (H&E) stain, acid fast stain and Gram stain. To detect the presence of specific microorganism in the tissues, the genomic DNA was extracted and polymerase chain reaction (PCR) was carried out. Results: Grossly, liver appeared as most affected organ followed by lungs, kidney and spleen. Wide spread necrosis and pipe steam liver due to fascioliasis (n=4), hydatidosis in lungs (n=3), congestion in the kidney (n=4) and petechial hemorrhage (n=2) in the spleen were the predominant lesions observed in the slaughtered cattle. H&E staining of tissue sections revealed granulomas in two liver and one lungs. Lung section stained with acid fast stain detected the acid fast bacilli in a case. Tissue sections stained with Gram stain showed cocci bacteria in the liver of two cattle. The PCR confirmed the presence of Mycobacterium tuberculosis in a cattle. Conclusions: Fascioliasis, hydatidosis and important zoonotic disease like tuberculosis are prevalent in the slaughtered cattle in Bangladesh. PCR technology appeared as a sensitive and specific screening test to diagnose tuberculosis. However, screening of large number of samples is necessary to detect the presence of important zoonotic diseases in cattle for safe beef production.

scholarly journals Development of specific primers for the detection of HVA1 from barley in transgenic durum wheat by polymerase chain reaction (PCR) technology

2014 ◽  
Vol 13 (4) ◽  
pp. 581-592 ◽  
Author(s):  
Melloul Marouane ◽  
Iraqi Driss ◽  
M Udupa Sripada ◽  
Abdelaziz El Alaoui My ◽  
Amine Alaoui Sanaa ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Durum Wheat ◽  
Chain Reaction ◽  
Specific Primers ◽  
Pcr Technology ◽  
Polymerase Chain

scholarly journals Prevalence and molecular detection of shiga toxin producing Escherichia coli from diarrheic cattle

2016 ◽  
Vol 14 (1) ◽  
pp. 63-68 ◽  
Author(s):  
MM Akter ◽  
S Majumder ◽  
KH MNH Nazir ◽  
M Rahman
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Molecular Detection ◽  
Chain Reaction ◽  
Specific Primers ◽  
Fecal Samples ◽  
E Coli ◽  
Uremic Syndrome ◽  
Polymerase Chain ◽  
Positive Isolate

Shiga toxin-producing Escherichia coli (STEC) are zoonotically important pathogen which causes hemorrhagic colitis, diarrhea, and hemolytic uremic syndrome in animals and humans. The present study was designed to isolate and identify the STEC from fecal samples of diarrheic cattle. A total of 35 diarrheic fecal samples were collected from Bangladesh Agricultural University (BAU) Veterinary Teaching Hospital. The samples were primarily examined for the detection of E. coli by cultural, morphological and biochemical characteristics, followed by confirmation of the isolates by Polymerase Chain Reaction (PCR) using gene specific primers. Later, the STEC were identified among the isolated E. coli through detection of Stx-1 and Stx-2 genes using duplex PCR. Out of 35 samples, 25 (71.43%) isolates were confirmed to be associated with E. coli, of which only 7 (28%) isolates were shiga toxin producers, and all of them were positive for Stx-1. However, no Stx-2 positive isolate could be detected. From this study, it may be concluded that cattle can act as a reservoir of STEC which may transmit to human or other animals.J. Bangladesh Agril. Univ. 14(1): 63-68, June 2016

scholarly journals Molecular detection and occurrence of equine theileriosis in Arabian horses in Al-Najaf province/Iraq

2020 ◽  
Vol 57 (3) ◽  
pp. e166996
Author(s):  
Hayder Mohammad Al-Rammahi ◽  
Abdulameer Abed Hatem ◽  
Asaad Chasib Al-Atabi
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Molecular Detection ◽  
Molecular Technique ◽  
Chain Reaction ◽  
Blood Samples ◽  
Equine Piroplasmosis ◽  
Polymerase Chain

This study was designed to detect equine piroplasmosis using the molecular technique in Al-Najaf province during the season that showed an increment in tick activities. Blood samples were collected from 110 horses with more than two signs of piroplasmosis. After DNA extraction, the product was examined by a polymerase chain reaction to amplify 18SrRNA. The results showed that the overall percentage of equine theileriosis was 38.18%. According to gender, the percentage of infection was 43.48% and 29.27% in females and males, respectively. Significant variations appeared between infected horses according to age, and the percentage of infection was 50% and 35.22% in less than 2 years and more than 2 years age, respectively. Moreover, the percentage of infection was 62.5% and 19.35% in animals with and without acariasis, respectively. Significant variations were also seen in equine theileriosis according to geographical areas, and the higher percentage was reported in Hera district (60.87%), while the lowest percentage was in the center of Al-Najaf (21.43%). This difference may be due to different distribution of vector of disease (tick), which may be the availability of the suitable weather that helped in the multiplication of the intermediate vectors. In conclusion, this study proved the variations in the occurrences of equine piroplasmosis according to gender, age, and geographical areas.

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