scholarly journals In vitro Study of Secreted Aspartyl Proteinases Sap1 to Sap3 and Sap4 to Sap6 Expression in Candida albicans Pleomorphic Forms

2012 ◽  
Vol 61 (4) ◽  
pp. 247-256 ◽  
Author(s):  
MONIKA STANISZEWSKA ◽  
MAŁGORZATA BONDARYK ◽  
KATARZYNA SIENNICKA ◽  
ANNA KUREK ◽  
JACEK ORŁOWSKI ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Human Serum ◽  
Associated Factors ◽  
In Vitro Study ◽  
Infection Process ◽  
Detection Level ◽  
Aspartic Proteases ◽  
Germ Tubes ◽  
Pleomorphic Cells

Transition from round budding cells to long hyphal forms and production of secreted aspartic proteases (Saps) are considered virulence-associated factors of Candida albicans. Although plenty of data dealing with Saps involvement in the infection process have been published, Saps expression by the different pleomorphic forms as well as the capacity of C. albicans filaments to express Sap1-6 under serum influence are poorly investigated. In this study, we used immunofluorescence and immunoelectron microscopy for the detection of Sap1-6 isoenzymes in C. albicans pleomorphic cells (blastoconidia, germ tubes, pseudohyphae, true hyphae) grown in Sap-inductive human serum and Sap non-inductive medium - yeast extract-peptone-glucose (YEPD). Isoenzymes were below the detection level in all blastoconidial cells grown in YEPD for 18 h. Sap1-6 expression was hardly detected in C. albicans cells cultivated in serum for 20 min. Increasing level of Sap1-6 expression was observed when C. albicans was incubated for 2, 6 and 18 h in serum corresponding to the development of germ tubes, pseudohyphae and true hyphae. The expression of Sap1-3 in pseudohyphae and true hyphae was more intensive compared to Sap4-6. Thus, we could show that human serum induced hyphae formation and the expression of Sap1-6 were co-regulated.

scholarly journals Ketorolac-fluconazole: A New Combination Reverting Resistance in Candida albicans from Acute Myeloid Leukemia Patients on Induction Chemotherapy: In vitro Study

2021 ◽  
Vol Volume 12 ◽  
pp. 465-474
Author(s):  
Shereen A Sayed ◽  
Ehsan AB Hassan ◽  
Muhamad R Abdel Hameed ◽  
Michael N Agban ◽  
Mostafa F Mohammed Saleh ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Candida Albicans ◽  
Induction Chemotherapy ◽  
Myeloid Leukemia ◽  
In Vitro Study ◽  
Vitro Study ◽  
New Combination ◽  
Acute Myeloid

scholarly journals Synergism of Streptococcus mutans and Candida albicans Reinforces Biofilm Maturation and Acidogenicity in Saliva: An In Vitro Study

2021 ◽  
Vol 10 ◽  
Author(s):  
Hye-Eun Kim ◽  
Yuan Liu ◽  
Atul Dhall ◽  
Marwa Bawazir ◽  
Hyun Koo ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Dental Caries ◽  
Streptococcus Mutans ◽  
Critical Role ◽  
In Vitro Study ◽  
Acidic Ph ◽  
Acidic Environment ◽  
Symbiotic Interaction ◽  
Biofilm Development

Early childhood caries, a virulent-form of dental caries, is painful, difficult, and costly to treat that has been associated with high levels of Streptococcus mutans (Sm) and Candida albicans (Ca) in plaque-biofilms on teeth. These microorganisms appear to develop a symbiotic cross-kingdom interaction that amplifies the virulence of plaque-biofilms. Although biofilm studies reveal synergistic bacterial-fungal association, how these organisms modulate cross-kingdom biofilm formation and enhance its virulence in the presence of saliva remain largely unknown. Here, we compared the properties of Sm and Sm-Ca biofilms cultured in saliva by examining the biofilm structural organization and capability to sustain an acidic pH environment conducive to enamel demineralization. Intriguingly, Sm-Ca biofilm is rapidly matured and maintained acidic pH-values (~4.3), while Sm biofilm development was retarded and failed to create an acidic environment when cultured in saliva. In turn, the human enamel slab surface was severely demineralized by Sm-Ca biofilms, while there was minimal damage to the enamel surface by Sm biofilm. Interestingly, Sm-Ca biofilms exhibited an acidic environment regardless of their hyphal formation ability. Our data reveal the critical role of symbiotic interaction between S. mutans and C. albicans in human saliva in the context of pathogenesis of dental caries, which may explain how the cross-kingdom interaction contributes to enhanced virulence of plaque-biofilm in the oral cavity.

Antifungal activity of four commercially available intense sweeteners against candida albicans - An In vitro study

2013 ◽  
Vol 3 (2) ◽  
pp. 60
Author(s):  
Subramaniam Ramanarayanan ◽  
Sakeenabi Basha ◽  
Mahesh Hiregoudar ◽  
PrashantGoudar Manjunath ◽  
Simpy Mittal ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals The Effectiveness of Chlorhexidine and Air Polishing System in the Treatment of Candida albicans Infected Dental Implants: An Experimental In Vitro Study

Antibiotics ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 179 ◽  
Author(s):  
Pier Carmine Passarelli ◽  
Marta De Leonardis ◽  
Giovan Battista Piccirillo ◽  
Viviana Desantis ◽  
Raffaele Papa ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Sodium Bicarbonate ◽  
Rough Surfaces ◽  
In Vitro Study ◽  
Titanium Implants ◽  
Vitro Study ◽  
Term Survival ◽  
Air Polishing ◽  
Scanning Electron

Background: Peri-implantitis is an inflammatory disease with an increasing diffusion rate which can affect the long-term survival of a prosthetic rehabilitation. The present study focused on the decontaminating efficacy of chlorhexidine and air polishing system with sodium bicarbonate powder against Candida albicans, a microorganism which seems to have a superinfecting opportunistic role in the pathology. The aim of the authors was to investigate and compare the effectiveness of these treatments, commonly used in clinical practice. Methods: An in vitro study was conducted to analyze the effects of two widely used therapeutic aids for the disinfection of affected titanium implants: chlorhexidine (CHX) and air polishing with sodium bicarbonate powder (P). A qualitative and quantitative comparative analysis of the residual biofilm was carried out using a colorimetric assay (XTT) and scanning electron microscopy (SEM) observation. The experiment was conducted both on machined titanium surfaces and on rough sandblasted ones with the aim of bringing out differences in the therapeutic outcomes concerning the superficial texture of the implant. The null hypothesis was that no difference could be detected between the samples, regarding both the treatments performed and the nano-structural features of titanium. Results: The best results (on both types of implant surfaces) were obtained when combining the use of chlorhexidine and air polishing (C + P). A linear decrease in the optical density (OD) values recorded at three different time points (30 s, 1 min, 5 min) was also observed passing from the first to the last one. When observed under scanning electron microscope rough surfaces showed an extensive and highly structured biofilm, more complex if compared to the one encountered when analyzing machined implants. Conclusions: the present pilot study showed that rough surfaces can promote fungal adhesion and eventually hinder the outcome of a decontaminating treatment. For this purpose, the physio-chemical technique is always more efficient if compared to a single-technique approach regardless of the surface characteristics.

In vitro study on the interaction of methoxyflurane with human serum albumin: Phenotypic characterization

2013 ◽  
Vol 153 ◽  
pp. 107-113 ◽  
Author(s):  
Aning Xing ◽  
Bin Jia ◽  
Xuan Li ◽  
Zhaodi Zhang ◽  
Guangying Gu ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
In Vitro Study ◽  
Vitro Study ◽  
Phenotypic Characterization

ChemInform Abstract: SYNTHESIS AND IN VITRO STUDY OF THYMIDINE AND HYPOXANTHINE CONJUGATES WITH MICROAGGREGATES OF HUMAN SERUM ALBUMIN

1980 ◽  
Vol 11 (39) ◽  
Author(s):  
N. A. BRUSENTSOV ◽  
V. M. BUKHMAN ◽  
G. A. BELITSKII ◽  
E. G. SLAVINA ◽  
I. L. LEIPUNSKAYA ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
In Vitro Study ◽  
Vitro Study
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