scholarly journals Bioremediation of Spent Engine Oil Contaminated Soils Using Indigenous Fungi Species

2020 ◽  
pp. 445-461
Author(s):  
Laurelta Tudararao-Aherobo ◽  
Solomon Mesogboriwon
Keyword(s):  
Contaminated Soils ◽  
Internal Combustion Engines ◽  
Engine Oil ◽  
Lubricating Oil ◽  
Test Duration ◽  
Aspergillus Glaucus ◽  
Talaromyces Flavus ◽  
Short Period ◽  
Spent Engine Oil ◽  
Set Up

Spent engine oil is derived from lubricating oil which has been used to lubricate various internal combustion engines and it is drained out for disposal during servicing of the engine. Spent engine oil causes great damage to soil and soil microflora when disposed indiscriminately. Thus, the bioremediation of spent engine oil contaminated soil was studied using indigenous degrading fungi isolated from hydrocarbon contaminated soils obtained from automobile mechanic workshops located at both Okpe and Uvwie Local Government Areas of Delta State, in the Niger Delta region of Nigeria. Three (3) fungi isolates with high engine oil biodegradability potential were used for the spent engine oil (SEO) bioremediation study. The fungi isolates used for the test were identified as, <em>Aspergillus glaucus</em>, <em>Trichoderma polysporum</em> and <em>Talaromyces flavus</em>using the API 20C method. The test microcosms were incubated for four weeks at 28 ± 2<sup>o</sup>C. Physicochemical parameters such as, Sulphate concentrations, Total petroleum hydrocarbon, Nitrate concentrations, Phosphate concentrations, Total organic carbon content, pH and Hydrocarbon utilizing fungi counts were monitored weekly using standard ASTM methods to assess the biodegradation of the spent engine oil. At the end of the test duration, <em>Talaromyces flavus </em>recorded the highest percentage spent engine oil biodegradation (69.66%) for the 5% SEO experimental set up. Similarly, <em>Aspergillus glaucus </em>recorded the highest percentage SEO biodegradation (66.16%) for the 10% experimental set up. Thus, <em>Talaromyces flavus </em>and <em>Aspergillus glaucus </em>could be used to effectively bioaugment the bioremediation process of spent engine oil contaminated soils to restore the soil to its original state within a short period of time.

scholarly journals Optimization of nutrients requirements for bioremediation of spent-engine oil contaminated soils

2018 ◽  
Vol 24 (3) ◽  
pp. 484-494
Author(s):  
Gabriel O. Ogbeh ◽  
Titus O. Tsokar ◽  
Emmanuel Salifu
Keyword(s):  
Contaminated Soils ◽  
Engine Oil ◽  
Spent Engine Oil

scholarly journals Potentials of African Star Apple (Chrysophyllum albidum) Fruit Shell Adsorbent in Recovery of Valuable Hydrocarbons for Spent Engine Oil

2021 ◽  
Vol 20 ◽  
pp. 9-22
Author(s):  
Elijah Taiwo ◽  
Oluwatosin Tokede ◽  
Olayinka Sanda
Keyword(s):  
Agricultural Waste ◽  
Fixed Bed ◽  
Renewable Resource ◽  
Apple Fruit ◽  
Engine Oil ◽  
Lubricating Oil ◽  
Fixed Bed Adsorption ◽  
Spent Engine Oil ◽  
Adsorption Processes ◽  
African Star Apple

Inventory of spent engine oil continuously increase and indiscriminately disposed of at auto-mechanics garages. Adsorptive separation established to be indispensable in recovery of these hydrocarbons was carried out using Chrysophyllum albidum (African star apple) fruit shell – a renewable resource. In this study, clay pretreated spent engine oil was recovered in a fixed bed adsorption processes and the recovered adsorbate components determined by GC-MS analysis. The results showed African star apple fruit shell adsorbent effective in recovering n-hexane solubilized spent lubricating oil. The study concluded that using the developed African star apple fruit shell adsorbent, an agricultural waste adsorbent in the recovery of waste lubricating oil will enhance greatly the nation’s economy.

scholarly journals Bioremediation of Spent Engine Oil on Selected Contaminated Soils within Ilorin Metropolis

2020 ◽  
Vol 8 (1) ◽  
pp. 91-104
Author(s):  
Elizabeth Adeyinka AJIBOYE ◽  
Hikmat Omolara SULAYMAN ◽  
Abdullahi Taiwo AJAO
Keyword(s):  
Heavy Metals ◽  
Organic Matter ◽  
Organic Carbon ◽  
Contaminated Soils ◽  
Physicochemical Parameters ◽  
Soil Samples ◽  
Engine Oil ◽  
Biochemical Tests ◽  
Spent Engine Oil ◽  
Before And After

The research aimed to investigate the bioremediation of spent engine oil on selected contaminated soils within Ilorin metropolis. To achieve this, soil samples were collected from three (3) mechanic workshops along Taiwo axis within the metropolis. The soil samples were then subjected to bioremediation using the land-farming approach. The physicochemical parameters of the soil samples before and after bioremediation were analyzed using standard methods. Bacteria were isolated using standard procedures and identified using biochemical tests and molecular methods. Results for the physicochemical parameters of the soil samples before bioremediation include particle size (all sandy in nature); pH (6.00 ± 0.14 - 6.20 ± 0.14); Organic carbon (14.65 ± 3.20 - 17.54 ± 1.87), Organic matter (33.50 ± 0.85 - 43.45 ± 9.12) and heavy metals (ND - 11.74 ± 0.07). Values after bioremediation for pH, organic carbon, organic matter and heavy metals were 8.25 ± 0.07 - 8.90 ± 0.14, 13.07 ± 0.05 - 13.25 ± 0.84, 37.25 ± 1.06 - 44.80 ± 1.13, ND - 9.40 ± 0.04 respectively. Values for bacterial count before and after bioremediation of the soil samples were 8.00  1.41 - 67.50 ± 2.12 x 105 CFU/mL and 6.50 ± 2.12 - 164.00 ± 11.31 x 105 CFU/mL respectively. Bacterial isolates were identified as Pseudomonas sp., Enterobacter sp., Acinetobacter sp., and Bacillus sp. while the hydrocarbon-utilizing bacteria were identified as Thalassospira mesophila strain JCM 18969; Pseudomonas fluorescens F113; Siccibacter turicensis LMG 23730; Pseudomonas Zeshuii strain KACC 15471; Pseudomonas stutzeri strain CGMCC 1.1803 and Marinobacter hydrocarbonoclasticus strain ATCC 49840. In conclusion, the bacteria isolates effectively bioremediated the spent engine oil contaminated soils with a reduction of hydrocarbon pollutants.

Influence of waste of lubricating oil on the intensity of its aging in a marine trunk diesel engine

2021 ◽  
pp. 56-65
Author(s):  
М.И. Тарасов ◽  
Г.П. Кича ◽  
А.В. Надежкин
Keyword(s):  
Diesel Engine ◽  
Internal Combustion Engines ◽  
Fuel Oil ◽  
Engine Oil ◽  
Lubricating Oil ◽  
Engine Fuel ◽  
Operating Life ◽  
Resource Saving ◽  
Aging Model ◽  
Operating Modes

Представлены результаты моделирования старения моторного масла в судовом тронковом дизеле при разном его угаре с идентификацией влияния на степень окисления, срабатывание присадок (по щелочности), накопление нерастворимых продуктов, рост кислотности и смолообразования форсировки двигателя и качества применяемых горюче-смазочных материалов. Показана рациональность исследования процесса старения смазочного масла в циркуляционной системе смазки одноцилиндрового отсека дизеля в лабораторных условиях, что позволяет выдерживать контролируемые параметры комплекса «дизель – топливо – масло» (ДТМ) и снизить затраты на моторные испытания. По результатам расчетно-эксперементального исследования получена модель старения смазочного масла по удельным, приходящимся на единицу мощности двигателя показателям, которая позволяет прогнозировать его состояние в зависимости от срока службы и своевременно отбраковывать. Показана адекватность модели старения по экспериментальным данным использования моторного масла в судовом полноразмерном тронковом дизеле повышенной форсировки. Разработанная модель по указанным направлениям старения позволяет определить состав и режимы функционирования комплекса ДТМ, при которых обеспечивается ресурсосберегающее маслоиспользование в двигателях внутреннего сгорания. This paper presents the results of an engine oil aging model in a marine trunk diesel engine under different combustion conditions with characterization of the effect on the oxidation rate, the actuation of additives (by alkalinity), the accumulation of non-soluble products, an increase in acidity and gumming of the engine boost and the quality of the utilized fuels and lubricants. The efficiency of the lubricating oil aging process study in the circulating lubrication system of a single-cylinder diesel compartment in laboratory conditions is also shown in the article, this allows to maintain the controlled parameters of the «diesel – fuel – oil» system and to reduce the cost of motor tests. Based on the results of a computational-experimental study a lubricating oil aging model was obtained in terms of specific indicators per unit of engine power, which allows to predict its condition depending on the operating life and to promptly discard it. The adequacy of the aging model is based on experimental data of the engine oil use in a ship's full-size trunk diesel engine with increased boost. The developed model of the indicated directions of aging makes it possible to determine the composition and operating modes of the «engine-fuel-oil» system, which ensures resource-saving oil use in internal combustion engines.

Gravimetric Profile of Hydrocarbon Degrading Bacterial and Fungal Isolates from Contaminated Soil Samples in Ado-Ekiti, Nigeria

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Olayinka O. Idris ◽  
◽  
Olayinka T. Ogunmefun ◽  
Cinderella N. Tuesimi
Keyword(s):  
Crude Oil ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Soil Samples ◽  
Engine Oil ◽  
Water Retention Capacity ◽  
Trichoderma Spp ◽  
Fungal Isolates ◽  
Degrading Bacteria ◽  
Spent Engine Oil

One of the biological compounds limiting soil water retention capacity is oil when present due to its hydro-nature. However, some microorganisms exhibit the capacity to degrade oil as a source of carbon, whereby the soil quality is retained and enhanced. Hence, the gravimetric profile of hydrocarbon degrading bacteria and fungi isolated from oil contaminated soil samples was investigated. Soil samples were collected from surface and 10m depth from six different mechanic workshops and generator sites. The pour plate technique was used to isolate the microorganisms. All pure isolates were sub-cultured using Bushnell Haas agar and the isolated bacteria were identified by their morphological and biochemical characteristics. The soil samples pH range was 4.3 - 6.4. Bacteria isolated included Pseudomonas spp., Staphylococcus spp., Microccocus spp., Acinetobacter spp., and Bacillus spp. The fungi isolated included Aspergillus spp., Rhizopus spp., Candida spp., Trichoderma spp. and Penicillium spp. Degradation of kerosene, diesel, crude oil, engine oil, and spent engine oil was allowed using Acinetobacter baumanni, P. aeruginosa, B. subtilis, and S. aureus. Gravimetric analyses were used to determine the percentage of petroleum hydrocarbon degraded by bacterial isolates. The highest percentage of degradation was between P. aeruginosa and B. subtilis. Pseudomonas aeruginosa degraded 97.4% diesel, 88.2% kerosene, 71.3% crude oil, 80.7% engine oil and 78.2% spent engine oil; while Bacillus subtilis degraded 71% diesel, 97% kerosene, 89.6% crude oil, 87% engine oil and 72.6% spent engine oil. This study revealed that bacterial and fungal isolates from oil contaminated soils exhibited the potentials to degrade oil and bioremediation using these microorganisms was possible.

scholarly journals METHODS OF INCREASING EFFICIENCY OF THIN CLEARING ENGINE OIL BY IMPROVING FILTERING NONWOVENS IN OIL PURIFIERS OF MARINE DIESEL ENGINS

2018 ◽  
pp. 31-41
Author(s):  
Andrei Veniaminovich Nadezkin ◽  
Gennadiy Petrovich Kicha ◽  
Liudmila Anatolievna Semeniuk
Keyword(s):  
Service Life ◽  
Diesel Engines ◽  
Internal Combustion Engines ◽  
Specific Capacity ◽  
Engine Oil ◽  
Lubricating Oil ◽  
Resource Saving ◽  
Marine Diesel ◽  
Oil Purification ◽  
Filtering Element

The article presents the results of motor tests of mass production filtering elements and new ones with improved performance in marine diesel engines. The filtering elements help to increase the efficiency of cleaning the engine oil and to lessen the engine wear and carbonation. The extended diesel service life is directly connected to the quality of oil purification. There has been carried out the analysis of developing filter technology regarding to engine oil cleaning in lubrication systems of ship medium-speed internal combustion engines equipped with different oil cleaners on the vessels of the Far Eastern basin. There have been presented the methods of increasing the throughput, dirt capacity and service life, as well as the fineness and completeness of sifting filtering elements due to the use of new filter materials. The dependence of the oil filtration rate on the efficiency of its purification is shown. Oil purification process can be intensified by means of decreasing the rate of oil filtering. To obtain the optimal filtering rate it is necessary to choose the appropriate size of a filtering element. Nowadays, the production technology of filtering elements is constantly improving, parameters of filtering materials are being upgraded, such as porosity, specific capacity, etc. There have been proposed measures to improve the hydraulic and filtering characteristics of nonwovens from cellulose and synthetic fibers of different thicknesses with different fillers and binders, which increases the permeability of papers, the number of pores per unit surface and a narrower distribution in diameter. New scientific and technical solutions are presented, which increase the efficiency of oil use in diesel engines. The ways of reducing the wear rate of the main parts in the engine due to full-flow fine cleaning of lubricating oil providing economical resource-saving operation of the trunk diesel engines are shown. The Russian industry manufactures the full-flow filtering elements and filtering materials relevant to the best international analogues.

scholarly journals Enhanced Biodegradation of Spent Engine Oil Contaminated Soil Using Organic Wastes

2016 ◽  
pp. 27-38 ◽  
Author(s):  
Tolulope M. Obuotor ◽  
Abdulwasiu O. Sakariyau ◽  
Babatunde S. Bada
Keyword(s):  
Contaminated Soil ◽  
Contaminated Soils ◽  
Burkholderia Cepacia ◽  
Micrococcus Luteus ◽  
Bacterial Count ◽  
Organic Wastes ◽  
Engine Oil ◽  
Gas Chromatography Mass Spectrometry ◽  
Candida Spp ◽  
Spent Engine Oil

Physical and chemical methods of remediating contaminated soils are less environment-friendly compared to the biodegradation method. This study investigated the ability of selected organic wastes to enhance biodegradation of Spent Engine Oil (SEO) contaminated soil. One kilogram of uncontaminated soil was thoroughly mixed with 10% (w/v) SEO in seven treatments with two replicates. Spent Fruit Residues (SFR), Cassava Peel (CP) and a combination of Bean Husk and Chromolaena odorata(BHC) were added at 10% and20% (w/w), with an untreated control. Total Heterotrophic Bacterial Count (THBC), Total Fungal Count (TFC), Total Hydro-carbon Degrading Bacterial Count (THDBC) and Total Hydrocarbon Degrading Fungal Count (THDFC) of the contaminated and uncontaminated soils were determined using standard micro-biological methods. Isolates were screened for SEO utilization using 2,6-dichlorophenol indo-phenol indicator. Hydrocarbon contents of the soils were determined using Gas Chromatography-Mass Spectrometry (GC-MS). The data obtained were subjected to statistical analysis. The THBC ranged from 1.3x106to 2.9x106CFU g-1, TFC ranged from 5.4x104to 2.0x105CFUg-1, THDBC ranged from 0.5x103to 1.9x104CFUg-1while THDFC ranged from 2.0x103to 1.0x104CFUg-1. The isolated bacteria were Pseudomonas spp., Bacillus spp., Klebsiella spp., Proteus mirabilis, Burkholderia cepacia, Micrococcus luteus, Providencia rettgeri, Enterococcus faecalis, Streptococcus bovisand Enterobacter cloacaewhile the isolated fungi were Candida spp., Aspergillus niger, Saccharomyces cerevisae, Penicillium chrysogenumand Trichophyton sp. Pseudomonas aeruginosaand Aspergillus nigerutilized the oil better than other isolates with absorbance of 0.26 and 0.49 at 600 nm, respectively. The GC-MS revealed that SFR 20% (w/w) treatment had the highest percentage degradation of 70.5%. This study confirms that spent fruit residues can enhance biodegradation of spent engine oil contaminated soil.

Yield and quality of maize from spent engine oil contaminated soils amended with compost under screenhouse conditions

2013 ◽  
Vol 30 (1) ◽  
pp. 9-19 ◽  
Author(s):  
Moses Bamidele Adewole ◽  
Adejumoke Oluwatoyin Aboyeji
Keyword(s):  
Contaminated Soils ◽  
Poultry Manure ◽  
Nutritional Composition ◽  
Engine Oil ◽  
Crude Fibre ◽  
Yield And Quality ◽  
Randomized Design ◽  
Uptake Rates ◽  
Spent Engine Oil

Abstract The study investigated the effects of poultry manure compost (PMC) on the growth performance and yield of maize (Zea mays L.) when cultivated on spent engine oil (SEO) contaminated soil with a view to assessing its nutritional composition. The experiment consisted of three SEO treatments (0%, 1% and 2% w/w) with six levels (0, 2, 4, 6, 8 and 10 t ha-1) of PMC applications. Each treatment was replicated thrice and arranged in a 3 × 6 × 3 factorial completely randomized design to give a total of 54 pots. At full physiological maturity, maize grains were harvested and threshed manually for analysis. As the bioavailability of metals increased with increasing PMC, their uptake by maize plants was enhanced. The soil amended with 10 t ha-1 and contaminated with 1% SEO recorded the highest uptake rates of 1.761 × 10-2, 2.345 × 10-2 and 4.285 × 10-2 day-1 for Fe, Cu and Pb respectively. Also, the significantly (P<0.05) highest yield of maize (5.8 t ha-1) and the highest nutritional values of crude protein (11.27 g 100 g-1) and crude fibre (2.71 g 100 g-1) were obtained with zero SEO soil contamination at 10 t ha-1 PMC applications. There was evidence of yield and nutritional quality reduction of maize when cultivated on SEO contaminated soils with or without poultry compost fertilization

scholarly journals Phytoremediation of heavy metals in spent engine oil-polluted soil by Senna alata L.

2019 ◽  
Author(s):  
C. Ugwu Emmanuel ◽  
O. Nwadinigwe Alfreda ◽  
C. Agbo Benita
Keyword(s):  
Heavy Metals ◽  
Dry Weight ◽  
Engine Oil ◽  
Reproductive Parameters ◽  
Flame Atomic Absorption ◽  
Randomized Design ◽  
Aerial Roots ◽  
Spent Engine Oil ◽  
Set Up ◽  
Senna Alata

AbstractSenna alata L. was used to remediate heavy metals in soil polluted with spent engine oil (SEO). One hundred and twenty polythene bags filled with 20 kg of soil each were separated into parts A and B. Part A contained S. alata seedlings while part B had no plant. They were set up in completely randomized design. To simulate spillage, 0.15% v/w, 0.75% v/w and 3.75% v/w concentrations of SEO were used to pollute soil planted with seeds of S. alata, 57 days after planting. These treatments were repeated in soil without seeds. Control had no pollution. Heavy metal analyses of SEO, vegetated and non-vegetated soil, leaves, stems and roots of S. alata were determined using Flame Atomic Absorption Spectroscopy, 106 days after pollution. Vegetative and reproductive parameters were also determined. Copper, Lead, Zinc, Iron and Aluminium were detected in SEO. Concentrations of heavy metals in vegetated soils were significantly (P < 0.05) less than those of non-vegetated soils. Cu accumulation in stem was significantly (P < 0.05) higher than those of leaves and roots. Zn and Al were significantly (P < 0.05) higher in root than those in leaves and stems. Fe and Pb were significantly (P < 0.05) higher in leaves than in stems and roots. Aerial roots were formed by S. alata which increased significantly with increase in concentrations of SEO applied. However, many vegetative parameters such as plant height, number of pinnules, number of roots, leaf area and stem circumference increased significantly (P < 0.05) but some reproductive parameters such as number of inflorescence and dry weight of seeds decreased after pollution. Hence, S. alata is suitable for phytoremediation and in particular, phytoaccumulation of heavy metals in SEO contaminated soil.

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