scholarly journals Pharmacological Study on Wild Variety of Coccinia indica

2019 ◽  
pp. 396-400
Author(s):  
V. Pushpa Rani ◽  
A. Anitha Nancy ◽  
K. Shanmuga Priya
Keyword(s):  
Aqueous Extract ◽  
Methanol Extract ◽  
Pharmacological Study ◽  
Dpph Assay ◽  
Abts Assay ◽  
Normal Variety ◽  
Effective Antioxidant ◽  
Wild Variety ◽  
Coccinia Indica ◽  
Anti Inflammatory

The current investigation dealt with pharmacological study of Coccinia indica (wild). For past several years, various medicinal plants were increasingly studied for the treatment of different ailments. The normal variety of C. indica was extensively used for home remedies and household purposes. But the wild variety of C. indica tastes bitter and showed good pharmacological activity. The current study revealed that ABTS assay was shown more scavenging activity than DPPH assay. The methanol extract of C. indica showed effective antioxidant activity than aqueous extract, but the property of anti inflammatory, aqueous extract of showed C. indica showed better anti inflammatory activity.

scholarly journals GC-MS analysis, evaluation of phytochemicals, anti-oxidant, thrombolytic and anti-inflammatory activities of Exacum bicolor

2015 ◽  
Vol 10 (4) ◽  
pp. 745 ◽  
Author(s):  
Appaji Mahesh Ashwini ◽  
Latha Puttarudrappa ◽  
Ravi Kumar ◽  
Mala Majumdar
Keyword(s):  
Methanol Extract ◽  
Clot Lysis ◽  
Total Phenolic ◽  
Dpph Assay ◽  
Thrombolytic Activity ◽  
Inflammatory Agent ◽  
Ms Analysis ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Alkaloid Contents

<p class="Abstract">The aim of the present study was to investigate the GC-MS analysis, phytochemical screening, anti-oxidant, thrombolytic and anti-inflammatory activities of methanol extract of leaves of Exacum bicolor. FTIR analysis confirmed the presence of alcohol, phenols, alkanes, aromatic compounds, aldehyde and ethers. GC-MS analysis revealed the presence of eight phyto-constituents. The total phenol, flavonoid and alkaloid contents were 18.0 ± 0.2 mg/GAE/g, 13.1 ± 0.4 mg QE/g and 108.0 ± 1.2 mg AE/g respectively. The DPPH assay exhibited potent anti-oxidant abilities with IC<sub>50 </sub>8.8 µg/mL. Significant thrombolytic activity was demonstrated by clot lysis method (45.1 ± 0.8%). The methanol extract showed significant membrane stabilization on human red blood cell with IC<sub>50 </sub>value of 37.4 µg/mL. There was a significant correlation (R<sup>2</sup>&gt;0.98) with total phenolic content versus anti-oxidant and anti-inflammatory activity. The above results confirmed that E. bicolor could be a promising anti-oxidant, thrombolytic and anti-inflammatory agent.</p><p> </p>

scholarly journals Anti-Inflammatory Effects of Aster incisus through the Inhibition of NF-κB, MAPK, and Akt Pathways in LPS-Stimulated RAW 264.7 Macrophages

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Daniel Ngabire ◽  
Yeong-Ae Seong ◽  
Maheshkumar Prakash Patil ◽  
Irvine Niyonizigiye ◽  
Yong Bae Seo ◽  
...  
Keyword(s):  
Western Blot ◽  
Methanol Extract ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Rt Pcr ◽  
Dpph Assay ◽  
Raw 264.7 Macrophages ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Almost All

Aster incisus is a common flower found in almost all regions of South Korea. In the current study, we investigated the potential antioxidant and anti-inflammatory properties of the Aster incisus methanol extract in LPS-stimulated RAW 264.7 cells. We analyzed the phytochemicals contained in the extract by GC-MS. GC-MS results showed that the Aster incisus extract contains 9 known compounds. Later on, DPPH assay, WST-1 assay, nitric oxide (NO) assay, Western blot, and RT-PCR were conducted to investigate the anti-inflammatory effects of the extract. Our WST-1 assay results revealed that Aster incisus did not affect the viability of all tested cell lines up to a concentration of 200 μg/ml; therefore, lower concentrations (50 μg/ml and 150 μg/ml) were used for further assays. Aster incisus scavenged DPPH and inhibited the production of NO. Aster incisus also reduced significantly the production of inflammation-related enzymes (iNOS, Cox-2) and cytokines (TNFα, IL-1β, and IL-6) and the gene expression of the proinflammatory cytokines. Additionally, further Western blot results indicated that Aster incisus inhibited the expression of p-PI3K, p-IκBα, p-p65 NF-κB, p-ERK1/2, p-SAPK/JNK, and p-Akt. Our results demonstrated that Aster incisus suppressed the expression of the inflammation mediators through the regulation of NF-κB, MAPK, and Akt pathways.

scholarly journals Antioxidant and antibacterial activities of Ischaemum indicum leaves extracted using different solvents

2019 ◽  
Vol 15 (3) ◽  
pp. 436-440
Author(s):  
Fithrotul Auwaliyah ◽  
Hamdan Dwi Rizqi ◽  
Adi Setyo Purnomo ◽  
Sri Fatmawati
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Methanol Extract ◽  
Antibacterial Activities ◽  
Dilution Method ◽  
Potential Candidate ◽  
Dpph Assay ◽  
Abts Assay ◽  
Water Extracts ◽  
Broth Dilution

The aim of this study was to evaluate antioxidant and antibacterial activities of Ischaemum indicum leaves extracts based on different polarity solvents: water, methanol, ethyl acetate, dichloromethane, and n-hexane. The antioxidant activity was evaluated using ABTS and DPPH methods, while the antibacterial activity was determined by broth dilution method against Pseudomonas aeruginosa and Bacillus subtilis. The results showed that water and methanol extracts of I. indicum gave the highest antioxidant activity in ABTS assay with an IC50 73.80 and 46.32 µg/mL, respectively. On the other hand, The IC50 on DPPH assay for  water extracts was 117.51 µg/mL, this value was higher than that of methanol extract with no inhibition. Methanol and water extracts showed the antibacterial activity against B. subtilis with inhibition of 48.62 and 45.11%, whereas inhibition of P. aeruginosa were 31.95 and 30.31%, respectively. This study denoted that I. indicum is a new potential candidate as antioxidant source.

scholarly journals Antioxidant Activity of Syzygium polyanthum Extracts

2017 ◽  
Vol 17 (1) ◽  
pp. 49 ◽  
Author(s):  
Mutia Devi Hidayati ◽  
Taslim Ersam ◽  
Kuniyoshi Shimizu ◽  
Sri Fatmawati
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Sulfonic Acid ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Dpph Assay ◽  
Abts Assay ◽  
Ic50 Value

Antioxidant activities of Syzygium polyanthum leaves extracts (methanol, ethyl acetate, dichloromethane and n-hexane) were evaluated by using DPPH (2,2-diphenyl-2-picrylhydrazyl) and ABTS (2,2’-azinobis (3-ethylbenzothiazole-6-sulfonic acid) methods. The methanol extract showed the highest antioxidant activity of DPPH assay among extract with IC50 value of 44.35 mg/mL. In addition, methanol extract also showed the highest antioxidant activity of ABTS assay among extracts with IC50 value of 17.69 mg/mL. This study indicated that the methanol extract of S. polyanthum leaves is potential as antioxidant.

scholarly journals Antioxidant, anti-inflammatory and antimicrobial activities of methanolic and aqueous extract of the leaves of Pistacia lentiscus L.

2020 ◽  
Vol 43.2 ◽  
pp. 7407-7414
Author(s):  
Nouioua Wafa ◽  
Gaamoune Sofiane
Keyword(s):  
Aqueous Extract ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Antimicrobial Activities ◽  
Pistacia Lentiscus ◽  
Total Dosage ◽  
Methanolic Extracts ◽  
Weak Activity ◽  
Medicinal Properties ◽  
Anti Inflammatory

1 ABSTRACT In this study, The Montaigne of Boutaleb contains a plant heritage characterized by the presence of several species that possess medicinal properties. The methanolic and aqueous extract of the leaves of Pistacia lentiscus were prepared, the yield of the methanolic extract was 2 .99 g the percentages 37.2% and the yield of the aqueous extract is 0.98 g with percentages 19.6%. The total dosage of polyphenols and flavonoids in the methanol extract was 38.27 ± 6.30 mg EAG/g extract and 9.80 ± 0,160, mg EAG/g extract, respectively. The total dosage of polyphenols and flavonoids in the aqueous extract was 26.76± 2.47 mg EAG/g extract and 4, 15±0.09 mg EAG/g extract, respectively. The effect of aqueous and methanolic extracts of mastic leaves were evaluated using the DPPH test. Both extracts have shown a weak trapping effect towards the free radicals with an IC50 68.27 ± 9.96 μg/ml and with an IC50 72.92 ± 9.43 μg/ml respectively, then weak activity antioxidant .The antibacterial activity of the methanolic and aqueous extract of the leaves of Pistacia lentiscus is significant. The aqueous and methanolic extract of mastic leaves possess a very strong anti-inflammatory activity to protect the membrane of human blood red blood cells.

Anti-inflammatory, analgesic and antipyretic activity of aqueous extract of fresh leaves of Coccinia indica

2009 ◽  
Vol 17 (4) ◽  
pp. 239-244 ◽  
Author(s):  
Junaid Niazi ◽  
Parabhdeep Singh ◽  
Yogita Bansal ◽  
R. K. Goel
Keyword(s):  
Aqueous Extract ◽  
Antipyretic Activity ◽  
Coccinia Indica ◽  
Anti Inflammatory

Anti-inflammatory effect of Mitragyna speciosa crude methanol extract on the guinea pig ileum

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
WM Shaik Mossadeq ◽  
K Syamimi ◽  
MP Azyyati ◽  
ZA Zakaria ◽  
AK Arifah ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Methanol Extract ◽  
Guinea Pig Ileum ◽  
Mitragyna Speciosa ◽  
Anti Inflammatory ◽  
Crude Methanol Extract ◽  
Inflammatory Effect

Turmeric and cinnamon dominate in antioxidant potential among four major spices

2017 ◽  
Vol 26 (1) ◽  
pp. 27 ◽  
Author(s):  
B Saranya ◽  
T Sulfikarali ◽  
S Chindhu ◽  
A M Muneeb ◽  
N K Leela ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Reducing Power ◽  
Black Pepper ◽  
Chloroform Extract ◽  
Antioxidant Potential ◽  
Total Phenol Content ◽  
Power Method ◽  
Phenol Content ◽  
Dpph Assay ◽  
Synthetic Antioxidant

Antioxidant activity of sequential extracts of black pepper, ginger, turmeric and cinnamon was determined by DPPH assay, phosphomolybdate method and ferric reducing power method and compared with that of the synthetic antioxidant BHA. The results revealed that methanol extract of cinnamon has highest antioxidant potential followed by chloroform extract of turmeric. The antioxidant potential was also correlated with total phenol content.  

Anti-inflammatory Effect of Allium hookeri Root Methanol Extract in LPS-induced RAW264.7 Cells

2012 ◽  
Vol 41 (11) ◽  
pp. 1645-1648 ◽  
Author(s):  
Chang-Hyun Kim ◽  
Mi-Ai Lee ◽  
Tae-Woon Kim ◽  
Ja Young Jang ◽  
Hyun Ju Kim
Keyword(s):  
Methanol Extract ◽  
Raw264.7 Cells ◽  
Allium Hookeri ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Comparative Analysis of In-Vitro Biological Activities of Methyl Eugenol Rich Cymbopogon khasianus Hack., Leaf Essential Oil with Pure Methyl Eugenol Compound

2020 ◽  
Vol 21 (10) ◽  
pp. 927-938 ◽  
Author(s):  
Roktim Gogoi ◽  
Rikraj Loying ◽  
Neelav Sarma ◽  
Twahira Begum ◽  
Sudin K. Pandey ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Biological Activities ◽  
Methyl Eugenol ◽  
Dpph Assay ◽  
Pure Compound ◽  
Allium Cepa Assay ◽  
Anti Inflammatory ◽  
Lemongrass Essential Oil

Background: The essential oil of methyl eugenol rich Cymbopogon khasianus Hack. was evaluated and its bioactivities were compared with pure methyl eugenol. So far, methyl eugenol rich essential oil of lemongrass was not studied for any biological activities; hence, the present study was conducted. Objective: This study examined the chemical composition of essential oil of methyl eugenol rich Cymbopogon khasianus Hack., and evaluated its antioxidant, anti-inflammatory, antimicrobial, and herbicidal properties and genotoxicity, which were compared with pure compound, methyl eugenol. Material and Methods: Methyl eugenol rich variety of Cymbopogon khasianus Hack., with registration no. INGR18037 (c.v. Jor Lab L-9) was collected from experimental farm CSIR-NEIST, Jorhat, Assam (26.7378°N, 94.1570°E). The essential oil wasobtained by hydro-distillation using a Clevenger apparatus. The chemical composition of the essential oil was evaluated using GC/MS analysis and its antioxidant (DPPH assay, reducing power assay), anti-inflammatory (Egg albumin denaturation assay), and antimicrobial (Disc diffusion assay, MIC) properties, seed germination effect and genotoxicity (Allium cepa assay) were studied and compared with pure Methyl Eugenol compound (ME). Results: Major components detected in the Essential Oil (EO) through Gas chromatography/mass spectroscopy analysis were methyl eugenol (73.17%) and β-myrcene (8.58%). A total of 35components were detected with a total identified area percentage of 98.34%. DPPH assay revealed considerable antioxidant activity of methyl eugenol rich lemongrass essential oil (IC50= 2.263 μg/mL), which is lower than standard ascorbic acid (IC50 2.58 μg/mL), and higher than standard Methyl Eugenol (ME) (IC50 2.253 μg/mL). Methyl eugenol rich lemongrass EO showed IC50 38.00 μg/mL, ME 36.44 μg/mL, and sodium diclofenac 22.76 μg/mL, in in-vitro anti-inflammatory test. Moderate antimicrobial activity towards the 8 tested microbes was shown by methyl eugenol rich lemongrass essential oil whose effectiveness against the microbes was less as compared to pure ME standard. Seed germination assay further revealed the herbicidal properties of methyl eugenol rich essential oil. Moreover, Allium cepa assay revealed moderate genotoxicity of the essential oil. Conclusion: This paper compared the antioxidant, anti-inflammatory, antimicrobial, genotoxicity and herbicidal activities of methyl eugenol rich lemongrass with pure methyl eugenol. This methyl eugenol rich lemongrass variety can be used as an alternative of methyl eugenol pure compound. Hence, the essential oil of this variety has the potential of developing cost-effective, easily available antioxidative/ antimicrobial drugs but its use should be under the safety range of methyl eugenol and needs further clinical trials.

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