scholarly journals Effect of virus inducible cis-element insertion on transcription properties of improved GWSF promoter in Arabidopsis thaliana

2020 ◽  
Vol 64 ◽  
pp. 320-323
Author(s):  
Z.C. HUANG ◽  
H. LI
Keyword(s):  
Arabidopsis Thaliana ◽  
Element Insertion ◽  
Cis Element

One novel cis-element is essential for correct DYSFUNCTIONAL TAPETUM 1 (DYT1) expression in Arabidopsis thaliana

2015 ◽  
Vol 34 (10) ◽  
pp. 1773-1780 ◽  
Author(s):  
Zhou Shumin ◽  
Chen Yan ◽  
Sun Hulin ◽  
Zheng Bang ◽  
Sun Licheng ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cis Element

scholarly journals Transcription Factor ANAC074 Binds to NRS1, NRS2, or MybSt1 Element in Addition to the NACRS to Regulate Gene Expression

2018 ◽  
Vol 19 (10) ◽  
pp. 3271
Author(s):  
Lin He ◽  
Jingyu Xu ◽  
Yucheng Wang ◽  
Kejun Yang
Keyword(s):  
Gene Expression ◽  
Arabidopsis Thaliana ◽  
Transcription Factor ◽  
Transcription Factors ◽  
Biological Processes ◽  
Regulate Gene Expression ◽  
Cis Element ◽  
Stress Responsive Genes ◽  
Regulate Gene ◽  
Gus Assays

NAC (NAM, ATAF1/2, and CUC2) transcription factors play important roles in many biological processes, and mainly bind to the NACRS with core sequences “CACG” or “CATGTG” to regulate gene expression. However, whether NAC proteins can bind to other motifs without these core sequences remains unknown. In this study, we employed a Transcription Factor-Centered Yeast one Hybrid (TF-Centered Y1H) screen to study the motifs recognized by ANAC074. In addition to the NACRS core cis-element, we identified that ANAC074 could bind to MybSt1, NRS1, and NRS2. Y1H and GUS assays showed that ANAC074 could bind the promoters of ethylene responsive genes and stress responsive genes via the NRS1, NRS2, or MybSt1 element. ChIP study further confirmed that the bindings of ANAC074 to MybSt1, NRS1, and NRS2 actually occurred in Arabidopsis. Furthermore, ten NAC proteins from different NAC subfamilies in Arabidopsis thaliana were selected and confirmed to bind to the MybSt1, NRS1, and NRS2 motifs, indicating that they are recognized commonly by NACs. These findings will help us to further reveal the functions of NAC proteins.

scholarly journals FUNCTIONAL ANALYSIS OF THE PERSICARIA MINOR SESQUITERPENE SYNTHASE GENE PROMOTER IN TRANSGENIC ARABIDOPSIS THALIANA

2019 ◽  
Vol 81 (4) ◽  
Author(s):  
Aimi Farehah Omar ◽  
Ismanizan Ismail
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Stress ◽  
Promoter Activity ◽  
Regulatory Element ◽  
Regulatory Elements ◽  
Sesquiterpene Synthase ◽  
Functional Region ◽  
Cis Element ◽  
Specific Manner ◽  
Persicaria Minor

Sesquiterpene synthase is an enzyme involved in sesquiterpene biosynthesis which catalyzed sesquiterpene formation from farnesyl diphosphate (FDP). In this research, the sesquiterpene synthase promoter (PmSS) was isolated from Persicaria minor (P.minor) to identify the functional region of the promoter and possible cis - regulatory element involved in the regulation of sesquiterpene synthase gene. Various putative cis - regulatory element involved in environmental stress and hormones were identified on PmSS promoter. The PmSS promoter and three series of deletion promoter were fused to β-glucuronidase (gus) gene and transformed into Arabidopsis thaliana. This study showed PmSS promoter was regulated in a developmental -specific manner and response to wounding, drought, heat, abscisic acid (ABA) and methyl jasmonate (MeJa) treatment. The results revealed the existence of cis regulatory elements that control the regulation of promoter activity in a developmental specific manner at -1758 to -1078 promoter sequences. The presence of cis-element acting as a repressor is expected to be present at the promoter between -1540 to -1078 bp. The region from -1078 to -855 was critical for maximal PmSS promoter activity. Deletion of promoter region from -1758 to -855 induced regulation of promoter in an organ-specific manner. Drought stress treatment did not induced GUS activity in deleted ABRE motif construct, suggested that ABRE motif is essential cis element during drought stress.  

Stable genetic transformation of Arabidopsis thaliana by Agrobacterium inoculation in planta

1994 ◽  
Vol 5 (4) ◽  
pp. 551-558 ◽  
Author(s):  
Seok So Chang ◽  
Soon Ki Park ◽  
Byung Chul Kim ◽  
Bong Joong Kang ◽  
Dal Ung Kim ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Genetic Transformation ◽  
In Planta

The effect of daylength on the transition to flowering in phytochrome-deficient, late-flowering and double mutants of Arabidopsis thaliana

1995 ◽  
Vol 95 (2) ◽  
pp. 260-266 ◽  
Author(s):  
Maarten Koornneef ◽  
Corrie Hanhart ◽  
Patty van Loenen-Martinet ◽  
Hetty Blankestijn de Vries
Keyword(s):  
Arabidopsis Thaliana ◽  
Late Flowering ◽  
Transition To Flowering

Identification of a single-copy gene encoding a Type I chlorophyll a/b-binding polypeptide of photosystem I in Arabidopsis thaliana

1992 ◽  
Vol 84 (4) ◽  
pp. 561-567 ◽  
Author(s):  
Poul E. Jensen ◽  
Michael Kristensen ◽  
Tine Hoff ◽  
Jan Lehmbeck ◽  
Bjarne M. Stummann ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Chlorophyll A ◽  
Photosystem I ◽  
Single Copy ◽  
Type I ◽  
Single Copy Gene ◽  
Gene Encoding ◽  
Copy Gene
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