scholarly journals Salt tolerance of potato genetically engineered with the Atriplex canescens BADH gene

2020 ◽  
Vol 64 ◽  
pp. 271-279 ◽  
Author(s):  
A. ALI ◽  
Q. ALI ◽  
M.S. IQBAL ◽  
I.A. NASIR ◽  
X. WANG
Keyword(s):  
Salt Tolerance ◽  
Genetically Engineered ◽  
Atriplex Canescens ◽  
Badh Gene

scholarly journals Efficient Bioproduction of Mycosporine-2-glycine, Which Functions as Potential Osmoprotectant, using Escherichia coli Cells

2017 ◽  
Vol 12 (10) ◽  
pp. 1934578X1701201 ◽  
Author(s):  
Tanutcha Patipong ◽  
Takashi Hibino ◽  
Rungaroon Waditee-Sirisattha ◽  
Hakuto Kageyama
Keyword(s):  
Escherichia Coli ◽  
Salt Tolerance ◽  
Maximum Level ◽  
Genetically Engineered ◽  
Synthetic Genes ◽  
E Coli ◽  
Escherichia Coli Cells ◽  
Nacl Concentration ◽  
Effective Growth ◽  
Halotolerant Cyanobacterium

Mycosporine-2-glycine (M2G) is known to be synthesized in halotolerant cyanobacterium Aphanothece halophytica. Escherichia coli cells in which the M2G synthetic genes of A. halophytica were introduced could synthesize M2G. Here, we report that M2G producing transformed E. coli cells showed salt tolerance compared to control cells. This result suggested that M2G could function as a potential osmoprotectant in E. coli. To our knowledge, this is the first report presenting the evidence that mycosporine-like amino acid confers salt tolerance on E. coli. Intracellular M2G content in the transformed E. coli cells were varied depending on NaCl concentration with maximum level at 0.75 M. Moreover, intracellular M2G level was affected by a supply of glycine with maximum level at 5 mM. In conclusion, we found that transformed E. coli cells could produce 205 μg of M2G/g fresh weight of cells under the best effective growth condition in this study. Thus, the results obtained here offer the potential for the bioproduction of mycosporine-like amino acids using the genetically engineered E. coli cells.

scholarly journals Betaine Aldehyde Dehydrogenase (BADH) gene and free amino acid analysis in Rhizophora mucronata Lam. from Thalassery region of Kerala, India.

2018 ◽  
Vol 7 (10) ◽  
pp. 2430
Author(s):  
Anusha Sreeshan ◽  
Meera S.P. ◽  
Anu Augustine
Keyword(s):  
Amino Acids ◽  
Salt Tolerance ◽  
Aldehyde Dehydrogenase ◽  
Free Amino Acids ◽  
Free Amino ◽  
Betaine Aldehyde Dehydrogenase ◽  
Chromatographic Technique ◽  
Rhizophora Mucronata ◽  
Betaine Aldehyde ◽  
Badh Gene

Mangroves are salt tolerant plants inhabiting saline environment. Multiple factors contribute to their salt tolerance and we need multifaceted approach to reveal the mechanisms of salt tolerance in the plant. In the present study, leaves of the mangrove, Rhizophora mucronata grown in the presence and absence of salt were used, free amino acids and the expression of selected genes were analyzed. Chromatographic technique showed the accumulation of free amino acids like proline, glycine, aspartic acid, valine, leucine and glutamic acid in the presence of salt. RNA was isolated from the leaf sample and cDNA was synthesized. Gene specific primers were designed and standardized. Among the genes studied (P5CS, BADH, NHX1), Betaine Aldehyde dehydrogenase (BADH) gene was found to be expressed.

Transformation of tomato with the BADH gene from Atriplex improves salt tolerance

2002 ◽  
Vol 21 (2) ◽  
pp. 141-146 ◽  
Author(s):  
Zhu Z.-Q. ◽  
Chang F.-Q. ◽  
Li Y.-X. ◽  
Jia G.-X.
Keyword(s):  
Salt Tolerance ◽  
Badh Gene

How much sodium accumulation is necessary for salt tolerance in subspecies of the halophyte Atriplex canescens?

1994 ◽  
Vol 17 (6) ◽  
pp. 711-719 ◽  
Author(s):  
E. P. GLENN ◽  
M. OLSEN ◽  
R. FRYE ◽  
D. MOORE ◽  
S. MIYAMOTO
Keyword(s):  
Salt Tolerance ◽  
Atriplex Canescens ◽  
Sodium Accumulation

scholarly journals Overexpression of TaBADH increases salt tolerance in Arabidopsis

2019 ◽  
Vol 99 (4) ◽  
pp. 546-555 ◽  
Author(s):  
Yinglu Sun ◽  
Xin Liu ◽  
Lianshuang Fu ◽  
Peng Qin ◽  
Tong Li ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Root Length ◽  
Soil Salinization ◽  
Betaine Aldehyde Dehydrogenase ◽  
Wild Type ◽  
Breeding Method ◽  
Wild Type Line ◽  
Transgenic Lines ◽  
Plant Salt Tolerance ◽  
Badh Gene

Soil salinization is an important threat to wheat growth and production. Previous transcriptome analysis showed that the expression of the betaine aldehyde dehydrogenase (BADH) gene differed significantly between cultivars with strong or weak salinity tolerance. Herein, the BADH gene from the wheat cultivar Dongnongdongmai 1 was cloned and transformed into wild-type Arabidopsis to identify its function in salt tolerance. Root length was calculated at 0, 50, 100, 150, and 200 mmol L−1 NaCl for 7 d. The relative electrolytic leakage (REL), GB content, and BADH activity were measured at 150 mmol L−1 NaCl for 1 and 3 d. It was determined that BADH activity and the GB content of TaBADH-overexpressed transgenic (TaBADHOE) lines were significantly higher than in wild-type lines. Salt stress analysis showed that the root length of TaBADHOE lines 4, 18, and 19 were 0.44, 0.54, and 0.35 cm, respectively, which were significantly longer than the 0.24 cm roots of the wild-type line in the media containing 150 mmol L−1 NaCl for 7 d. In addition, the RELs of transgenic lines 4, 18, and 19 were 0.37, 0.33, and 0.42, respectively, which is significantly lower than the 0.63 of the wild-type line in media containing 150 mmol L−1 NaCl for 3 d. These results demonstrate that TaBADH significantly increased plant salt tolerance, indicating that genetic transformation of TaBADH may be an effective and sustainable breeding method for increasing salt tolerance in wheat cultivars.

Sign in / Sign up

Export Citation Format

Share Document