scholarly journals Diagnosis of the microbiological quality of fruits and vegetables in Chihuahua, Mexico

Phyton ◽  
2008 ◽  
Vol 77 (1) ◽  
pp. 129-136
Author(s):  
羦ila-Quezada G ◽  
E S醤chez ◽  
E Mu駉z ◽  
LR Mart韓ez ◽  
E Villalobos
2007 ◽  
Vol 70 (5) ◽  
pp. 1277-1280 ◽  
Author(s):  
HANS FRÖDER ◽  
CECÍLIA GERALDES MARTINS ◽  
KATIA LEANI OLIVEIRA de SOUZA ◽  
MARIZA LANDGRAF ◽  
BERNADETTE D. G. M. FRANCO ◽  
...  

The increasing demand for fresh fruits and vegetables and for convenience foods is causing an expansion of the market share for minimally processed vegetables. Among the more common pathogenic microorganisms that can be transmitted to humans by these products are Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella. The aim of this study was to evaluate the microbial quality of a selection of minimally processed vegetables. A total of 181 samples of minimally processed leafy salads were collected from retailers in the city of Sao Paulo, Brazil. Counts of total coliforms, fecal coliforms, Enterobacteriaceae, psychrotrophic microorganisms, and Salmonella were conducted for 133 samples. L. monocytogenes was assessed in 181 samples using the BAX System and by plating the enrichment broth onto Palcam and Oxford agars. Suspected Listeria colonies were submitted to classical biochemical tests. Populations of psychrotrophic microorganisms >106 CFU/g were found in 51% of the 133 samples, and Enterobacteriaceae populations between 105 and 106 CFU/g were found in 42% of the samples. Fecal coliform concentrations higher than 102 CFU/g (Brazilian standard) were found in 97 (73%) of the samples, and Salmonella was detected in 4 (3%) of the samples. Two of the Salmonella-positive samples had <102 CFU/g concentrations of fecal coliforms. L. monocytogenes was detected in only 1 (0.6%) of the 181 samples examined. This positive sample was simultaneously detected by both methods. The other Listeria species identified by plating were L. welshimeri (one sample of curly lettuce) and L. innocua (2 samples of watercress). The results indicate that minimally processed vegetables had poor microbiological quality, and these products could be a vehicle for pathogens such as Salmonella and L. monocytogenes.


2021 ◽  
Vol 319 ◽  
pp. 01112
Author(s):  
Rahma Erahioui ◽  
Sultana Inekach ◽  
Hassna Jaber ◽  
Khadija Atfaoui ◽  
Noureddine Rhaim ◽  
...  

Fruits and vegetables are essential for human health due to their nutritional qualities, but the consumption of raw foods leads to food safety problems as they are recognized as sources of transmission of infectious diseases. The objective of this work is to assess the microbiological quality of some fruits and vegetables marketed in the Kenitra city. The samples were purchased at the fruit and vegetable markets during the period from April to July 2018.The detection of microorganisms in the samples requires several steps which are weighing, dilution, isolation, enumeration and 'identification. The results of the microbiological analysis of fruits and vegetables studied show significant concentrations of the total aerobic mesophilic flora varies between 2.25 and 8.48 log10 CFU / ml, Concerning total coliforms the contamination rates vary between 1.92 and 8.42 log10UFC / ml, as well as the presence of fecal coliforms between 1.13 and 8.05 log10UFC / ml, in the same samples analyzed, strains such as: Escherichia coli, staphylococcus aureus and a total absence of salmonella and shegilla. In order to improve the safety and hygienic quality of fruits and vegetables, the application of good hygienic practices as well as continuous microbial quality control is necessary to protect the health of consumers.


2017 ◽  
Vol 11 (5) ◽  
pp. 185-193 ◽  
Author(s):  
Victor Ntuli ◽  
Peter Chatanga ◽  
Raphael Kwiri ◽  
Henry Tendekayi Gadaga ◽  
Jephris Gere ◽  
...  

Author(s):  
LAURA MARIA BRUNO ◽  
ANA AMÉLIA MARTINS DE QUEIROZ ◽  
ANA PAULA COLARES DE ANDRADE ◽  
NATÁLIA MOURA DE VASCONCELOS ◽  
MARIA DE FÁTIMA BORGES

Avaliou-se a qualidade microbiológica de 15 amostras de hortaliças/tubérculos e de 15 amostras de frutas minimamente processadas, comercializadas em Fortaleza (CE). Foram realizadas análises de coliformes fecais e totais, pesquisa de Salmonella sp., contagem total de bolores e leveduras, contagem de Staphylococcus coagulase positiva e contagem total de psicrotróficos. Detectou-se a presença de Salmonella em 66,6% das amostras de hortaliças/tubérculos e em 26% das de frutas. Foi verificado que 13,3% das amostras de hortaliças/ tubérculos apresentaram contagem de coliformes fecais acima do limite estabelecido pela legislação brasileira. Contagens elevadas de coliformes totais, de bolores e leveduras e de psicrotróficos também foram encontradas em ambos os produtos, indicando condições inadequadas de higiene durante o processamento, comprometendo seu armazenamento e sua qualidade microbiológica. MICROBIOLOGICAL EVALUATION OF VEGETABLES AND FRUITS MINIMALLY PROCESSED MARKETED IN FORTALEZA (CE) Abstract The microbiological quality of 15 vegetables and 15 fruits minimally processed samples marketed in Fortaleza - CE (Brazil) was evaluated. The following analyses were carried out: total and fecal coliforms, Salmonella sp., total yeasts and moulds count, coagulase positive Staphylococcus count and total psychrotrophics count. Salmonella was detected in 66.6% of vegetable samples and 26% of fruit samples. Fecal coliforms count higher than legislation limit, it was verified in 13.3% of vegetable samples. High counts of total coliforms, yeasts and moulds and psychrotrophics were also found on both products, showing inadequate hygiene conditions during manufacturing, affecting storage and microbiological quality of minimally processed fruits and vegetables.


Author(s):  
Patience C. Obinna-Echem

Aims: Physicochemical and microbiological quality of fruits and vegetables from open market and shopping mall in Port Harcourt metropolis were determined to ascertain the effect of the sales environment. Methodology: pH and titratable acidity (TTA) of bananas (Musa sapientum), tomatoes (Lycopersicon esculentum Mill), lettuce (Lactuca sativa L) and cabbage (Brassica oleracae var. capitata L) were determined using standard analytical methods. Conventional microbiological method was used for the microbial analysis. Results: pH (4.30 - 6.00) and TTA (0.05 - 0.28% lactic acid) of samples from the two locations did not differ significantly (P>0.05). Aerobic colony count (ACC), Coliform, Escherichia coli and Staphylococcus aureus, ranged from 4.49 – 5.96, 3.70 – 4.59, 3.69 - 4.39 and 3.68 – 4.17 Log10 CFU/g respectively for the shopping mall samples, and 6.14 - 6.19, 3.66 – 5.23, 4.60 – 5.66, and 3.69 – 5.15 Log10 CFU/g respectively for the open market samples. Salmonella was detected at a level of 3.74, 4.30 and 4.65 Log10 CFU/g respectively in banana from the shopping mall; and lettuce and cabbage from the open market. Mould growth for the open market samples ranged from 4.01 - 4.40 Log10 CFU/g. Shopping mall samples had no mould and yeast growth except for mould count of 4.29 Log10 CFU/g in banana and yeast count of 4.30 and 3.69 Log10 CFU/g in tomatoes and lettuce. Banana and lettuce from the open market had no yeast count, but tomatoes and cabbage had counts of 4.30 and 4.46 Log10 CFU/g respectively. Conclusion: Fruits and vegetables from the shopping mall generally had lower microbial count indicating the influence of the chilled storage environment. The detection of pathogens in the fruits and vegetables is unsatisfactory and can pose a health risk to consumers as well as the contamination of other produce.


2005 ◽  
Vol 30 (03) ◽  
Author(s):  
J Kehr ◽  
B Morales ◽  
P Contreras ◽  
L Castillo ◽  
W Aranda

2019 ◽  
Vol 485 (2) ◽  
pp. 229-233
Author(s):  
V. P. Kalyabina ◽  
E. N. Esimbekova ◽  
I. G. Torgashina ◽  
K. V. Kopylova ◽  
V. A. Kratasyuk

We formulated the principles of designing bioluminescent enzyme tests for assessing the quality of complex media which consist in providing the maximum sensitivity to potentially toxic chemicals at a minimal impact of uncontaminated complex media. The developed principles served as a basis for designing a new bioluminescent method for an integrated rapid assessment of chemical safety of fruits and vegetables which is based on using the luminescent bacterium enzymes (NAD(P)H:FMN oxidoreductase and luciferase) as a test system.


Domiati cheese is the most popular brand of cheese ripened in brine in the Middle East in terms of consumed quantities. This study was performed to investigate the impact of the microbiological quality of the used raw materials, the applied traditional processing techniques and ripening period on the quality and safety of the produced cheese. Three hundred random composite samples were collected from three factories at Fayoum Governorate, Egypt. Collected samples represent twenty-five each of: raw milk, table salt, calf rennet, microbial rennet, water, environmental air, whey, fresh cheese, ripened cheese & swabs from: worker hands; cheese molds and utensils; tanks. All samples were examined microbiologically for Standard Plate Count (SPC), coliforms count, Staphylococcus aureus (S. aureus) count, total yeast & mould count, presence of E. coli, Salmonellae and Listeria monocytogenes (L. monocytogenes). The mean value of SPC, coliforms, S. aureus and total yeast & mould counts ranged from (79×102 CFU/m3 for air to 13×108 CFU/g for fresh cheese), (7×102 MPN/ cm2 for tank swabs to 80×106 MPN/ml for raw milk), (9×102 CFU/g for salt to 69×106 CFU/g for fresh cheese) and (2×102 CFU/cm2 for hand swabs to 60×104 CFU/g for fresh cheese), respectively. Whereas, E. coli, Salmonella and L. monocytogenes failed to be detected in all examined samples. There were significant differences in all determined microbiological parameters (p ≤0.05) between fresh and ripened cheese which may be attributed to different adverse conditions such as water activity, pH, salt content and temperature carried out to improve the quality of the product.


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