scholarly journals Fumaria parviflora improves liver damage and lipid profile changes in STZ-induced diabetic rats

2020 ◽  
Vol 24 (3) ◽  
pp. 221-229
Author(s):  
Fateme Kooshki ◽  
◽  
Hamid Reza Niazkar ◽  
Shabnam Shirazi ◽  
Vahid Asghari Azar ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Lipid Profile ◽  
Serum Levels ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Control Group ◽  
Stress Markers ◽  
Alternative Drug ◽  
Male Wistar Rats ◽  
Profile Changes

Introduction: Chemical drugs and herbal medicine play a significant role in the management of diabetes mellitus complications. The aim of this study was to investigate the effects of Fumaria parviflora as an herbal source and glibenclamide as a chemical drug, on the liver tissue of diabetic rats. Methods: Male Wistar rats (n=32) were classified randomly into four groups (8/group), including control, diabetic (induced by 50mg/kg streptozocin), 250mg/kg hydroalcoholic extract of Fumaria parviflora (DFP) and 5mg/kg glibenclamide groups. After 21 days of treatment, liver tissues and blood samples were stored at -80°C to test lipid profile, liver enzymes and some oxidative stress markers. Results: In the diabetic group compared to the control group, the activities of SOD and GPx and also the serum levels of alkaline phosphatase were significantly decreased, while the levels of malondialdehyde, alanine aminotransferase and aspartate transaminase enhanced. Treatment with DFP and glibenclamide could manage the levels of all mentioned-parameters. Furthermore, in both treated groups, the rate of damages in the liver of rats reduced compared to the diabetic group. Conclusion: According to the obtained results, the administration of DFP or glibenclamide could ameliorate the deleterious effects of diabetes mellitus on some investigated-parameters. As there were no difference between DFP and glibenclamide effects, Fumaria parviflora could be considered as an alternative drug, at least for the diabetic complications examined in this study. However, further investigation is needed.

scholarly journals Effects of l-Carnitine on the Follicle-Stimulating Hormone, Luteinizing Hormone, Testosterone, and Testicular Tissue Oxidative Stress Levels in Streptozotocin-Induced Diabetic Rats

2018 ◽  
Vol 23 ◽  
pp. 2515690X1879605 ◽  
Author(s):  
Nourollah Rezaei ◽  
Tahereh Mardanshahi ◽  
Majid Malekzadeh Shafaroudi ◽  
Saeed Abedian ◽  
Hamid Mohammadi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Serum Levels ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Control Group ◽  
Group Vi ◽  
Group I ◽  
Stimulating Hormone

The present study was designed to investigate the antioxidant property of l-carnitine (LC) on serum levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone (TH) and testis oxidative stress in streptozotocin (STZ)-induced diabetic rats. The rats were divided into the following groups: group I, control; group II, LC 100 mg/kg/d; group III, diabetic; and groups IV to VI, diabetic rats treated with 50, 100, and 200 mg/kg/d of LC, respectively. Daily injections were given intraperitoneally for 7 weeks. At the end of experimental period, after sacrificing the rats, FSH, LH, TH, total antioxidant capacity (TAC), malondialdehyde (MDA), glutathione (GSH), catalase (CAT), mitochondrial function (MTT), protein carbonyl (PC), and reactive oxygen species (ROS) levels were measured. STZ caused an elevation of MDA, ROS, and PC ( P < .001) with reduction of GSH, CAT, TAC, and MTT ( P < .001) in the serum levels. Group VI had significantly increased FSH, LH, and TH levels versus the untreated diabetic group ( P < .001). Although groups V and VI significantly decreased MDA ( P < .001), PC ( P < .01), and ROS ( P < .01) compared with the untreated diabetic group; only in group VI, the activity of GSH ( P < .001), CAT ( P < .01), TAC ( P < .001), and MTT ( P < .001) significantly increased. The results of the present study suggest that LC decreased diabetes-induced oxidative stress complications and also improved serum level of FSH, LH, and TH by reducing levels of lipid peroxidation and increasing antioxidant enzymes.

scholarly journals IMPROVEMENT IN OXIDATIVE STRESS AFTER DUODENOJEJUNOSTOMY IN AN EXPERIMENTAL MODEL OF TYPE 2 DIABETES MELLITUS

2016 ◽  
Vol 29 (suppl 1) ◽  
pp. 3-7 ◽  
Author(s):  
Cacio Ricardo WIETZYCOSKI ◽  
João Caetano Dallegrave MARCHESINI ◽  
Sultan AL-THEMYAT ◽  
Fabiola Shons MEYER ◽  
Manoel Roberto Maciel TRINDADE
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Control Group ◽  
Oral Glucose ◽  
Surgical Group

ABSTRACT Background: Type 2 Diabetes Mellitus is a multifactorial syndrome with severe complications. Oxidative stress is accepted as a causal factor of chronic complications Aim: To demonstrate alterations in oxidative stress after metabolic surgery. Methods: Twenty-four 2-day-old Wistar rats were used. In 16, Type 2 Diabetes Mellitus was induced by 100 mg/kg streptozotocin injection. The development of diabetes was confirmed after 10 weeks using an oral glucose tolerance test. Eight diabetic rats composed the diabetic surgical group; the remaining eight composed the diabetic group. Eight animals in which diabetes was not induced formed the clinical control group. The Marchesini technique was used in the diabetic surgical group. After 90 days, the rats were sacrificed, and the oxidative stress markers were measured. Results: Thiobarbituric acid reactive substances, superoxide dismutase and catalase were significantly reduced in the diabetic surgical group compared to the diabetic group. Conclusion: The duodenojejunostomy was effective in controlling the exacerbated oxidative stress present in diabetic rats.

scholarly journals Hypoglicemic and Hypolipedimic Effects of Ganoderma lucidum in Streptozotocin-Induced Diabetic Rats

Medicines ◽  
2018 ◽  
Vol 5 (3) ◽  
pp. 78 ◽  
Author(s):  
Erna Bach ◽  
Edgar Hi ◽  
Ana Martins ◽  
Paloma Nascimento ◽  
Nilsa Wadt
Keyword(s):  
Ganoderma Lucidum ◽  
Biochemical Analysis ◽  
Food Supplement ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Control Group ◽  
Histopathological Analysis ◽  
Beta Glucan ◽  
Male Wistar Rats ◽  
Normoglycemic Control

Background:Ganoderma lucidum (Leyss. Ex. Fr) Karst is a basidiomycete mushroom that has been used for many years as a food supplement and medicine. In Brazil, National Health Surveillance Agency (ANVISA) classified Ganoderma lucidum as a nutraceutical product. The objective of the present work was to observe the effects of an extract from Ganoderma lucidum in rats treated with streptozotocin, and an agent that induces diabetes. Method: Male Wistar rats were obtained from the animal lodging facilities of both University Nove de Julho (UNINOVE) and Lusiada Universitary Center (UNILUS) with approval from the Ethics Committee for Animal Research. Animals were separated into groups: (1) C: Normoglycemic control water; (2) CE: Normoglycemic control group that received hydroethanolic extract (GWA); (3) DM1 + GWA: Diabetic group that received extract GWA; and (4) DM1: Diabetic group that received water. The treatment was evaluated over a 30-day period. Food and water were weighted, and blood plasma biochemical analysis performed. Results: G. lucidum extract contained beta-glucan, proteins and phenols. Biochemical analysis indicated a decrease of plasma glycemic and lipid levels in DM rats induced with streptozotocin and treated with GWA extract. Histopathological analysis from pancreas of GWA-treated DM animals showed preservation of up to 50% of pancreatic islet total area when compared to the DM control group. In plasma, Kyn was present in diabetic rats, while in treated diabetic rats more Trp was detected. Conclusion: Evaluation from G. lucidum extract in STZ-hyperglycemic rats indicated that the extract possesses hypoglycemic and hypolipidemic activities. Support: Proj. CNPq 474681/201.

scholarly journals The Protective Effect of Marigold Hydroalcoholic Extract in STZ-Induced Diabetic Rats: Evaluation of Cardiac and Pancreatic Biomarkers in the Serum

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Esmaeel Ebrahimi ◽  
Saeed Shirali ◽  
Rahman Talaei
Keyword(s):  
Diabetes Mellitus ◽  
Protective Effect ◽  
Antioxidant Property ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Dependent Manner ◽  
Hydroalcoholic Extract ◽  
Diabetic Control Group ◽  
Male Wistar Rats

Diabetes mellitus is the most common endocrine disorder worldwide and it is usually along with complications such as retinopathy, neuropathy, nephropathy, and cardiovascular disease. The prevalence of diabetes is increasing and its treatment has created concerns in society. The use of herbal medicine can be helpful in the management of diabetes mellitus. The aim of this study was to investigate the protective effect of marigold hydroalcoholic extract under diabetes condition. A total of 36 male Wistar rats were randomly divided into four groups: normal control group, diabetic control group, and groups treated with 250 or 500 mg/kg hydroalcoholic extract of marigold flower during four weeks. At the end of the study, the rats were anesthetized with ketamine/xylazine, and sampling was performed through cardiac puncture. The results showed that treatment with marigold improved body weight. In addition, we determined that marigold normalized the level of CK-MB, total CK, amylase, and lipase in a dose-dependent manner. Probably these effects resulted from antioxidant property of marigold; thus we suggest that marigold flower can be useful for reduction of diabetes complication.

scholarly journals D-limonene in diabetic rats

2021 ◽  
Vol 10 (3) ◽  
pp. e24-e24
Author(s):  
Shahrokh Bagheri ◽  
Mostafa Moradi Sarabi ◽  
Mohammadreza Gholami ◽  
Vahideh Assadollahi ◽  
Reza Mohammadrezaei Khorramabadi ◽  
...  
Keyword(s):  
Real Time ◽  
Kidney Tissue ◽  
Serum Levels ◽  
Diabetic Control ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Tissue Homogenate ◽  
Control Group ◽  
Mrna Levels ◽  
Male Wistar Rats

Introduction: Diabetes mellitus (DM) is a multi-factorial condition associated with oxidative stress. Limonene, as a plant-derived antioxidant, can be used for treating DM. Objectives: An investigation on antioxidant effects in diabetic rats exposed to D-limonene. Materials and Methods: Sixty male Wistar rats were categorized into six groups as follows: control (healthy rats), diabetic control (untreated diabetic rats), sham glibenclamide, diabetic glibenclamide, sham limonene, and finally diabetic limonene. Alloxan (100 mg/dL) was infused intraperitoneally to induce type 1 diabetes in rats. Rats in certain groups were given limonene (100 mg/dL) and glibenclamide (10 mg/dL) orally for 8 weeks. Subsequently, animals were killed, and their kidneys were removed. Serum levels of biochemical factors (including serum creatinine, urea, and glucose) were determined, and factors such as nitric oxide (NO), malondialdehyde (MDA), glutathione (GSH), and myeloperoxidase (MPO) were measured in kidney tissue homogenate. The gene expression and enzymatic activity of glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD) in the kidney were measured by real-time polymerase chain reaction (real-time PCR) and spectrophotometry, respectively. Results: Limonene treatment significantly decreased serum glucose, creatinine, and urea. Additionally, MDA, MPO, and NO significantly decreased while GSH increased after treatment with limonene. Real-time RT-PCR showed significant elevation (P<0.05) in mRNA levels of GPx, CAT, and SOD in the limonene-treated compared with the diabetic control group. Conclusion: Our results demonstrated that limonene as an herbal antioxidant had better effects on antioxidant markers compared to glibenclamide in rat models of diabetes.

scholarly journals The Effect of Exercise Training and Portulaca Oleracea on Neurobehavioral Dysfunction in Type 2 Diabetic Rats

2021 ◽  
Author(s):  
Hesam Parsa ◽  
Tayebeh Shiravand ◽  
Kamal Ranjbar ◽  
Alireza Komaki
Keyword(s):  
Exercise Training ◽  
Diabetic Rats ◽  
Portulaca Oleracea ◽  
Diabetic Group ◽  
Male Rats ◽  
Control Group ◽  
Swimming Training ◽  
Plus Maze ◽  
Male Wistar Rats ◽  
Healthy Control

Abstract Background: Diabetes mellitus is one of the most important causes of Alzheimer’s disease and dementia. Portulaca oleracea (P.oleracea) is a rich source of antioxidants, which reduces inflammation and oxidative stress in diabetic rats. Exercise training has also been shown to improve mental function and enhance learning and memory efficacy. Therefore, this study was designed to explore the potential combined effect of P. oleracea and exercise training on neurobehavioral dysfunction in streptozotocin (STZ)-induced diabetic male rats. Methods: For this purpose, 50 male Wistar rats were divided into five groups: 1) healthy control group (Con), 2) sedentary diabetic group (D), 3) diabetic rats treated with P. oleracea(D+Po), 4) diabetic rats treated with exercise training (D+Ex), and 5) diabetic rats treated with P.oleracea and exercise training (D+Po+Ex) simultaneously. Animals in the exercise groups were subjected to progressive swimming training for 12weeks. P.oleracea was mixed with standard pellet food for 12weeks. Neurobehavioral dysfunction was investigated by elevated plus-maze, shuttle box, open field, and novel object recognition tests.Results: Compared with the normal control group, rats in the sedentary diabetic group showed a more passive avoidance memory deficit and more anxiety, and less exploration. Due to exercise training and treatment with P. oleracea, the neurobehavioral deficit in the trained diabetic rats receiving P. oleracea reached the normal levels of those in the healthy group.Conclusion: These data demonstrated that diabetes causes significant neurobehavioral deficit. Nevertheless, swimming training and P. oleracea synergistically ameliorate and reverse the neurobehavioral deficit in STZ-induced diabetic male rats.

scholarly journals Evaluating the Effects of Aloe vera Gel on Cerebellum Istomorphometrical Changes in Diabetic Male Rats

2021 ◽  
Vol 23 (3) ◽  
Author(s):  
Mahmood Khaksary Mahabady ◽  
Naeem Erfani Majd ◽  
Mohammad Bahrami Tapebur ◽  
Yazdan Mazaheri
Keyword(s):  
Normal Saline ◽  
Aloe Vera ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Male Rats ◽  
Control Group ◽  
Protective Effects ◽  
Neuroprotective Effects ◽  
Aloe Vera Gel ◽  
Male Wistar Rats

Background: Diabetes mellitus can lead to histomorphometrical changes in the brain. Recent studies have shown that Aloe vera gel has antioxidant and neuroprotective effects, which is independent of glucose-lowering effects. Objectives: The present study aimed to investigate the effects of A. vera gel on histomorphometrical changes of cerebellum following streptozotocin (STZ)-induced diabetic male rats. Methods: A total of 25 male Wistar rats were randomly allocated into five groups as follows: (1) the control group received normal saline; (2) A. vera gel group; (3) diabetic group (normal saline); (4) treatment group diabetic rats, which received A. vera; and (5) diabetic rats which received insulin. A single dose of STZ [60 mg/kg; intraperitoneal (IP)] was used for the induction of diabetes in rats. All the treatments were administered daily for eight weeks. Subsequently, histomorphometrical changes were evaluated in the cerebellum of the rats. Results: The results showed that the number of granular and purkinje cells reduced in the cerebellum granulosa region, while the number of glial cells increased in the molecular region of the cerebellum in diabetic rats compared to the control group (P < 0.05). These changes were improved in treated rats by insulin or A. vera. Also, the thickness of molecular, purkinje, granular, and white matter layers at the apex of lobules and depth of sulcus in the diabetic group had a significant reduction compared to other groups (P < 0.001). Conclusions: Our results confirmed that improvement of the cerebellar tissue changes in diabetic rats following the use of A. vera gel is comparable to insulin. However, more investigations are required to determine the protective effects of A. vera gel against diabetes-induced cerebellum histomorphometrical changes.

scholarly journals Morphological Changes of Gingiva in Streptozotocin Diabetic Rats

2009 ◽  
Vol 2009 ◽  
pp. 1-4 ◽  
Author(s):  
C. Tesseromatis ◽  
A. Kotsiou ◽  
H. Parara ◽  
E. Vairaktaris ◽  
M. Tsamouri
Keyword(s):  
Diabetes Mellitus ◽  
Morphological Changes ◽  
Diabetic Rats ◽  
Control Group ◽  
Diabetic Patients ◽  
Histological Investigation ◽  
Bacterial Diseases ◽  
Male Wistar Rats ◽  
Streptozotocin Induced Diabetes ◽  
Routine Assay

Gingivitis and periodontitis are chronic bacterial diseases of the underlying and surrounding tooth tissues. Diabetes mellitus is responsible for tooth deprivation both by decay and periodontal disease. The streptozotocin-induced diabetes results in a diabetic status in experimental animals similar to that observed in diabetes patients. The aim of the study was to investigate the relationship between the gingival lesions and the microangiopathy changes in streptozotocin-induced diabetes mellitus. Forty male Wistar rats were divided into two groups (control and experimental). Diabetes mellitus was induced by 45 mg/kg IV streptozotocin. The histological investigation of the marginal gingival and the relevant gingival papilla showed inflammation of the lamina propria and the squamous epithelium as well as marked thickness of the arteriole in the diabetic group, but no changes were observed in the control group. The results suggested a probable application of a routine gingival histological investigation in diabetic patients in order to control the progress of disease complications. It may be concluded that histological gingival investigation can be used as a routine assay for the control of the diabetic disease and prevention of its complications.

scholarly journals Black Seed Thymoquinone Improved Insulin Secretion, Hepatic Glycogen Storage, and Oxidative Stress in Streptozotocin-Induced Diabetic Male Wistar Rats

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Heba M. A. Abdelrazek ◽  
Omnia E. Kilany ◽  
Muhammad A. A. Muhammad ◽  
Hend M. Tag ◽  
Aaser M. Abdelazim
Keyword(s):  
Lipid Profile ◽  
Pancreatic Islet ◽  
Wistar Rats ◽  
Nigella Sativa ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Hepatic Glycogen ◽  
Therapeutic Effects ◽  
Male Wistar Rats

Diabetes mellitus is one of the metabolic diseases having several complications. Nigella sativa oil (NSO) might have beneficial effects in the treatment of diabetic complications. Thirty-two mature male Wistar rats were equally divided into four experimental groups: control, control NSO 2 mL/kg, streptozotocin- (STZ-) induced diabetic, and diabetic (STZ-induced) treated with oral NSO 2 mg/kg for 30 days. Fasting blood glucose (FBG), insulin, and lipid profile levels were determined. Pancreatic and hepatic tissues were used for catalase and GSH. Histopathology, hepatic glycogen contents, insulin immunohistochemistry, and pancreatic islet morphometry were performed. NSO 2 mL/kg was noticed to decrease (P<0.05) FBG and increase (P<0.05) insulin levels in diabetic rats than in diabetic nontreated animals. Lipid profile showed significant (P<0.5) improvement in diabetic rats that received NSO 2 mL/kg than in the diabetic group. Both pancreatic and hepatic catalase and GSH activities revealed a significant (P<0.05) increment in the diabetic group treated with NSO than in the diabetic animals. NSO improved the histopathological picture and hepatic glycogen contents of the diabetic group as well as increased (P<0.05) insulin immunoreactive parts % and mean pancreatic islet diameter. NSO exerts ameliorative and therapeutic effects on the STZ-induced diabetic male Wistar rats.

scholarly journals Structural Changes in the Urinary Bladder of Streptozotocin-Induced Diabetic Rats and the Possible Protective Role of Insulin

2020 ◽  
Vol 17 (3) ◽  
Author(s):  
Amir M. Bassam Elnagar ◽  
Suhaidah Ibrahim ◽  
Mostafa A. M. Abouelnaga ◽  
Amro Mohamed Soliman
Keyword(s):  
Diabetes Mellitus ◽  
Urinary Bladder ◽  
Protective Role ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Control Group ◽  
Diabetic Patients ◽  
Pathological Changes ◽  
Histopathological Changes

Introduction: Diabetes mellitus possesses severe adverse effects on the urinary bladder. Urinary bladder dysfunction is a common health problem affecting diabetic patients causing recurrent infections and urinary incontinence. Objective: To evaluate the histopathological changes in the tissue of urinary bladder in Streptozotocin (STZ) diabetic rats and the protective role of insulin. Methods: Thirty rats were classified into three groups: a control group which received no treatment (Group A), STZ diabetic group (Group B) and Insulin diabetic group (Group C). Animals were sacrificed after six weeks and urinary bladders were harvested and processed for light and electron microscopy. Results: Several histopathological changes were observed in the urinary bladder of the diabetic group including an increase in the thickness of the urothelium, epithelial cells with dark nuclei and large lenticular vesicles, and wide intercellular spaces with numerous collagen fibers. Treatment with insulin reduced the pathological changes induced by STZ. Conclusion: Diabetes mellitus caused significant pathological changes in the urinary bladder of experimental rats. For instance, treating diabetic animals with insulin prevented the development of damaging effects of diabetes on the urinary bladder.

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