scholarly journals Rapid isolation of rosmarinic acid from Ocimum basilicum using flash chromatography

2019 ◽  
Vol 3 (1) ◽  
pp. 72-76 ◽  
Author(s):  
Hüseyin Akşit ◽  
Mehmet Keçeci ◽  
İbrahim Demirtaş ◽  
Nusret Genç
Keyword(s):  
Rosmarinic Acid ◽  
Ocimum Basilicum ◽  
Flash Chromatography ◽  
Rapid Isolation

scholarly journals Saudi Rosmarinus officinalis and Ocimum basilicum L. Polyphenols and Biological Activities

Processes ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 446 ◽  
Author(s):  
Hosam O. Elansary ◽  
Agnieszka Szopa ◽  
Paweł Kubica ◽  
Halina Ekiert ◽  
Diaa O. El-Ansary ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Rosmarinic Acid ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Natural Populations ◽  
Ocimum Basilicum ◽  
Rosmarinus Officinalis ◽  
Leaf Extracts ◽  
Gentisic Acid ◽  
Dihydroxyphenylacetic Acid

Investigating the polyphenolic profile of natural Rosmarinus officinalis and Ocimum basilicum populations may reveal essential compounds that have biological activities. Natural populations of R. officinalis and O. basilicum in Northern Riyadh were investigated by HPLC-DAD analyses. Several polyphenols, including rosmarinic acid, gentisic acid, 3,4-dihydroxyphenylacetic acid, rutoside, and others, out of 38 screened were confirmed. Rosmarinic acid was the major polyphenol in both of R. officinalis and O. basilicum. R. officinalis methanolic leaf extracts contained other phenols such as gentisic acid while O. basilicum contained also 3,4-dihydroxyphenylacetic acid and rutoside as well as others. R. officinalis showed higher antioxidant activities than O. basilicum using 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and β-carotene bleaching assays. These higher activities are associated with a higher composition of rosmarinic acid in leaf extracts. The antioxidant activities of O. basilicum were attributed to identified phenols of rosmarinic acid, 3,4-dihydroxyphenylacetic acid, and rutoside. There were antiproliferative and cytotoxic activities of leaf extracts, as well as identified polyphenols, against several cancer cells. These activities were attributed to the accumulation of necrotic and apoptotic cells in treated cancer cells with leaf extracts as well as identified polyphenols. The antibacterial and antifungal activities of leaf extracts were mainly attributed to 3,4-dihydroxyphenylacetic acid and rutoside in O. basilicum and rosmarinic acid and caffeic acid in R. officinalis. This study proved that R. officinalis and O. basilicum natural populations might be considered as promising sources of natural polyphenols with biological activities.

scholarly journals Insight into Composition of Bioactive Phenolic Compounds in Leaves and Flowers of Green and Purple Basil

Plants ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 22 ◽  
Author(s):  
Bhakti Prinsi ◽  
Silvia Morgutti ◽  
Noemi Negrini ◽  
Franco Faoro ◽  
Luca Espen
Keyword(s):  
Phenolic Acids ◽  
Rosmarinic Acid ◽  
Antioxidant Properties ◽  
Morphological Characteristics ◽  
Ocimum Basilicum ◽  
Ornamental Plant ◽  
Low Degree ◽  
New Knowledge ◽  
Acylated Anthocyanins ◽  
Insight Into

Basil (Ocimum basilicum L.) is a culinary, medicinal, and ornamental plant appreciated for its antioxidant properties, mainly attributed to high content of rosmarinic acid. This species also includes purple varieties, characterized by the accumulation of anthocyanins in leaves and flowers. In this work, we compared the main morphological characteristics, the antioxidant capacity and the chemical composition in leaves, flowers, and corollas of green (‘Italiano Classico’) and purple (‘Red Rubin’ and ‘Dark Opal’) basil varieties. The LC-ESI-MS/MS analysis of individual compounds allowed quantifying 17 (poly)phenolic acids and 18 flavonoids, differently accumulated in leaves and flowers of the three varieties. The study revealed that in addition to rosmarinic acid, basil contains several members of the salvianolic acid family, only scarcely descripted in this species, as well as, especially in flowers, simple phenolic acids, such as 4-hydroxybenzoic acid and salvianic acid A. Moreover, the study revealed that purple leaves mainly contain highly acylated anthocyanins, while purple flowers accumulate anthocyanins with low degree of decoration. Overall, this study provides new biochemical information about the presence of not yet characterized bioactive compounds in basil that could contribute to boosting the use of this crop and to gaining new knowledge about the roles of these compounds in plant physiology.

scholarly journals Effect of Ocimum basilicum, Ocimum selloi, and Rosmarinic Acid on Cerebral Vascular Damage in a Chronic Hypertension Model

2019 ◽  
Vol 42 (2) ◽  
pp. 201-211
Author(s):  
Elian Alegría-Herrera ◽  
Maribel Herrera-Ruiz ◽  
Rubén Román-Ramos ◽  
Alejandro Zamilpa ◽  
Mayra Alejandra Santillán-Urquiza ◽  
...  
Keyword(s):  
Rosmarinic Acid ◽  
Ocimum Basilicum ◽  
Vascular Damage ◽  
Chronic Hypertension ◽  
Cerebral Vascular

Elite hairy roots of Ocimum basilicum as a new source of rosmarinic acid and antioxidants

2016 ◽  
Vol 126 (1) ◽  
pp. 19-32 ◽  
Author(s):  
Shivani Srivastava ◽  
Xavier A. Conlan ◽  
Alok Adholeya ◽  
David M. Cahill
Keyword(s):  
Hairy Roots ◽  
Rosmarinic Acid ◽  
Ocimum Basilicum

High Rosmarinic Acid Content in Induced Mutants and in in vitro Elicited Sweet Basil (Ocimum basilicum L.) Callus

2007 ◽  
Vol 6 (7) ◽  
pp. 1058-1064 ◽  
Author(s):  
Adel A.Guirgis . ◽  
Mostafa A. Abd El-Ka . ◽  
H.N. Abbas ◽  
Azza M.S. Araffa ◽  
Ahmed I. Maksoud .
Keyword(s):  
Acid Content ◽  
Rosmarinic Acid ◽  
Ocimum Basilicum ◽  
Sweet Basil ◽  
Induced Mutants

Scale-up micropropagation of sweet basil (Ocimum basilicum L.) in an airlift bioreactor and accumulation of rosmarinic acid

2004 ◽  
Vol 26 (6) ◽  
pp. 521-523 ◽  
Author(s):  
Spiridon Kintzios ◽  
Haralambos Kollias ◽  
Evangelos Straitouris ◽  
Olga Makri
Keyword(s):  
Rosmarinic Acid ◽  
Scale Up ◽  
Ocimum Basilicum ◽  
Airlift Bioreactor ◽  
Sweet Basil

Phytochemical Screening, Antiproliferative and Antioxidant Properties of Various Extracts from Endemic Origanum acutidens

2018 ◽  
Vol 21 (4) ◽  
pp. 281-291
Author(s):  
Feyza Oke-Altuntas ◽  
Mehmet Ali Demirci ◽  
Ibrahim Demirtas ◽  
Ayse Sahin Yaglioglu ◽  
Lutfi Behcet
Keyword(s):  
Phenolic Compounds ◽  
Hela Cell ◽  
Ethyl Acetate ◽  
Cell Line ◽  
Antiproliferative Activity ◽  
Rosmarinic Acid ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Flash Chromatography ◽  
Hela Cell Line

Aim and Objective: Origanum acutidens (Hand.–Mazz.) Ietsw. is an endemic and perennial plant grown mainly in East Anatolia. Recently, natural plant products have attracted interest due to their safety and therapeutic effects. Therefore, the aim of this study was to investigate phytochemical contents and biological effects of Origanum acutidens. Materials and Methods: The aerial parts of O. acutidens were extracted with water, ethyl acetate, nbutanol, and methanol/chloroform solvents. Phenolic compounds and other constituents of the extracts were analyzed by HPLC/TOF-MS. The Ethyl Acetate extract (EA) was fractionated by flash chromatography. The extracts and fractions were investigated for their antiproliferative activities on human cervical adenocarcinoma (HeLa) cell line by using BrdU ELISA assay. Antioxidant activities of the extracts and fractions were evaluated by complementary test systems, namely determination of total phenolic contents, metal chelating ability and DPPH radical scavenging assay. Results: Among the extracts, Ethyl Acetate extract (EA) exhibited the highest antiproliferative activity (IC50 = 15.71 ± 0.04 µg/mL) on HeLa cells. It was therefore fractionated by flash chromatography to obtain 10 fractions which were investigated for their phenolic compounds and bioactivities. Rosmarinic acid was determined as the major component of EA and its fractions. EA exhibited higher antiproliferative activity against HeLa cell line than its fractions and 5-fluorouracil (5-FU) at the concentration of 100 µg/mL. EA and its fractions (F10, F6, F4, F7, F3, and F2) displayed higher radical scavenging activity compared to Butylated Hydroxytoluene (BHT). These effects may be attributed to the presence of rosmarinic acid in EA and its active fractions. Conclusion: This study demonstrated that O. acutidens is an essential natural source of polyphenols and a potent natural antioxidant and antiproliferative agent for food and pharmaceutical industries.

scholarly journals Rosmarinic Acid and Related Phenolics in Adventitious Root Cultures of Ocimum basilicum L.

1996 ◽  
Vol 13 (1) ◽  
pp. 69-71 ◽  
Author(s):  
Hiromi TADA ◽  
Yoshihiro IKEDA ◽  
Toshio OMOTO ◽  
Koichiro SHIMOMURA ◽  
Kanji ISHIMARU
Keyword(s):  
Rosmarinic Acid ◽  
Adventitious Root ◽  
Ocimum Basilicum ◽  
Root Cultures ◽  
Adventitious Root Cultures

scholarly journals Bioactivity-guided isolation of rosmarinic acid as a principle bioactive compound from the butanol extract of Isodon rugosus against pea aphid, Acyrthosiphon pisum

2019 ◽  
Author(s):  
Saira Khan ◽  
Clauvis Nji Tizi Taning ◽  
Elias Bonneure ◽  
Sven Mangelinckx ◽  
Guy Smagghe ◽  
...  
Keyword(s):  
Insecticidal Activity ◽  
Rosmarinic Acid ◽  
Acyrthosiphon Pisum ◽  
Bioactive Compound ◽  
Reversed Phase ◽  
Pea Aphid ◽  
Flash Chromatography ◽  
Spectroscopic Techniques ◽  
Butanol Fraction ◽  
Isodon Rugosus

AbstractAphids are agricultural pest insects that transmit viruses and cause feeding damage on a global scale. Current pest control involving the excessive use of synthetic insecticides over decades has led to multiple forms of aphid resistance to most classes of insecticides. In nature, plants produce secondary metabolites during their interaction with insects and these metabolites can act as toxicants, antifeedants, anti-oviposition agents and deterrents towards the insects. In a previous study, we demonstrated that the butanol fraction from a crude methanolic extract of an important plant species, Isodon rugosus showed strong insecticidal activity against the pea aphid, Acyrthosiphon pisum. It was however not known as which compound was responsible for such activity. To further explore this finding, current study aimed to exploit a bioactivity-guided strategy to isolate and identify the active compound in the butanol fraction of I. rugosus. As such, reversed-phase flash chromatography, acidic extraction and different spectroscopic techniques were used to isolate and identify the new compound, rosmarinic acid as the bioactive compound in I. rugosus. Insecticidal activity of rosmarinic acid was carried out using standard protocols on A. pisum. The data was analyzed using qualitative and quantitative statistical approaches. Considering that a very low concentration of this compound (LC90 = 5.4 ppm) causes significant mortality in A. pisum within 24 h, rosmarinic acid could be exploited as a potent insecticide against this important pest insect. Furthermore, I. rugosus is already used for medicinal purposes and rosmarinic acid is known to reduce genotoxic effects induced by chemicals, hence it is expected to be safer compared to the current conventional pesticides. While this study highlights the potential of I. rugosus as a possible biopesticide source against A. pisum, it also provides the basis for further exploration and development of formulations for effective field application.

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