scholarly journals The Effect Of Giving Red Dragon Fruit (Hylocereus polyrhizus) Extract On Spermatozo Motility In White Rats (Rattus norvegicus) Wistar Line With High Fat Induction

2020 ◽  
Vol 6 (3) ◽  
pp. 51-62
Author(s):  
Kirani Nuranisa ◽  
Cut Fauziah ◽  
Yuli Suciati
Keyword(s):  
Negative Control Group ◽  
Negative Control ◽  
University Education ◽  
Control Group ◽  
High Fat ◽  
Fruit Extract ◽  
White Rats ◽  
Group I ◽  
Dragon Fruit ◽  
Hylocereus Polyrhizus

Hyperlipidemia is a condition when high level of blood lipids and causes morphologicalchanges in spermatozoa. Red dragon fruit (Hylocereus Polyrhizus) is one of the plantsas a source of antioxidants and has unsaturated fat content that is needed in thespermatozoa maturation process. The aims of this the study is to determine the benefitsof red dragon fruit extract on the motility of white rat spermatozoa (Rattus norvegius)induced by high-fat diet. The study is design by true experimental research andresearch only testing group design. This research conducted in the Pharmacology andTherapy Laboratory, Padjajaran University Education Hospital, in January 2020 usingwhite rats, male . ± 8 weeks wistar strains weighing ± 200-250. The study sample wasdivided into several groups such group I as a control, group II as a negative control,group III as positive group with simvastatin dose 0.72 mg / day, group IV as researchgroup 1 using red dragon fruit extract dose of 60 mg / 200 grBB / day for 52 days, andgroup V as group 2 therapy using dragon fruit extract dose of 60 mg / 200grBB / dayfor 104 days. The results showed that the mean percentage of motility of ratspermatozoa increased in the relief group with the supplement of red dragon fruitextract by dosing of 60 mg / 200 grBB / day for 104 days, or 51, 2%. This studyconclude that red dragon fruit has a superior effectivity better than simvastatin inincreasing the motility of spermatozoa.

scholarly journals Pemberian Ekstrak Buah Pare dan Diet Tinggi Lemak pada Penurunan Berat Badan Tikus Putih Jantan

2019 ◽  
Vol 17 (1) ◽  
pp. 60
Author(s):  
I MADE SUBHAWA HARSA
Keyword(s):  
Rattus Norvegicus ◽  
High Fat Diet ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Momordica Charantia ◽  
Control Group ◽  
Bitter Melon ◽  
High Fat ◽  
White Rats

<div class="Section1"><p align="center"><strong>Abstra</strong><strong>ct</strong></p><p><strong>Background</strong> : Obesity is an energy balance disorder, ketogenic diet can reduce body weight. Bitter melon (<em>Momordica charantia L.)</em> is a type of vegetable material that is often used as an antioxidant, hypocholesterolemia and hypotriglyceridemia drug. <em>Cucurbitasin</em> is a substance produced by bitter melon (<em>Momordica charantia L.)</em> with a bitter taste which causes in decreasing appetite, resulting in a decrease in triglycerides. This is caused by the bioactivity of bitter melon (<em>Momordica charantia L.)</em> which can increase lipid oxidation, which causes the accumulation of decreased fat tissue which can reduce weight.</p><p><strong>Methods</strong>: This research using pre and post test randomized controlled group design with sample size of 30 male white rats (<em>Rattus norvegicus</em>) wistar lines divided into 3 groups, group 1 as negative control group given standard feed, group 2 as positive control group given high-fat diet with a dose of 2.5 ml and group 3 as a treatment group given a high-fat feed with dose of 2.5 ml and extract of bitter melon (<em>Momordica charantia L.)</em> at dose of 37.5 mg / 150 gBB by feeding tube every morning for 28 days. Data were analyzed by T-paired tests.</p><p><strong>Result</strong>: The T-paired test in each group found a significantly different result of p = 0,000.</p><p><strong>Conclusion</strong>: given bitter melon (<em>Momordica charantia L.)</em> extract and high-fat diet can reduce weight in male white rats (<em>Rattus norvegicus</em>).</p><p> </p><p><strong>Keywords</strong>:Bitter molon extract, high fat feed, weight loss.</p><p> </p></div><strong><br clear="all" /></strong>

scholarly journals Effect of salam leaf extract (Syzygium polyanthum to decrease blood triglyceride white rats

2012 ◽  
Vol 10 (2) ◽  
pp. 40-45
Author(s):  
ANDHIKA AJI NUGROHO ◽  
NUR HAFIDHA HIKMAYANI ◽  
SUTARMIADJI DJUMAGA
Keyword(s):  
Triglyceride Level ◽  
Leaf Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
White Rats ◽  
Group I ◽  
Post Test ◽  
Group Ii ◽  
Post Hoc

Nugroho AA, Hikmayanti NH, Djumaga S. 2012. Effect of salam (Syzygium polyanthum) leaf extract to decrease blood triglyceride level on white rats. Biofarmasi 10: 40-45. This study aimed to determine the effect of bay [Syzygium polyanthum (Wight) Walp.] leaf extract to decrease the blood triglyceride level of white rats (Rattus norvegicus). This research was an laboratory experimental research with pre and post-test design with control group. This research used 30 Wistar white rats, with ±200 g body weight and the age of ±2 months, divided into 5 groups by a random sampling, six rats per group. Group I was a negative control, group II was a positive control, groups III, IV and V was treated with the bay leaf extract in the doses of I, II and III. All groups were fed of hyperkolemia, group II was treated by Gemfibrozil 20 mg/200 g BW/day, while groups III, IV and V were treated by the bay leaf extract with the doses of 0.18 g, 0.36 g and 0.72 g/200 g BW/day. The study was conducted for 35 days. On the 28th and 35th day, the blood of all rats was taken from orbital vein for blood triglyceride levels measured in pre-test and post-test. The data were statistically analyzed with One-Way ANOVA test and a post-hoc test. There were significant differences between pre-test and post-test in the levels of blood triglycerides of white rats (p<0.001), in which a post-hoc test results showed the comparison between negative control and Gemfibrozil was p<0.001, and the comparison between the groups of the bay leaf extract with Gemfibrozil was p>0.05, and the comparison of three doses of bay leaf extract was p>0.05. The provision of bay leaf extract could reduce the blood triglyceride levels of white rats significantly compared with the negative control, whereas the effects of three doses of bay leaf extract was comparable with the effects of Gemfibrozil.

scholarly journals Antiproliferation Effects of Nanophytosome-Loaded Phenolic Compounds From Fruit of Juniperus Polycarpos Against Breast Cancer in Mice Model: Synthesis, Characterization and Therapeutic Effects

2021 ◽  
Author(s):  
Soheila Moeini ◽  
Ehsan Karimi ◽  
Ehsan Oskoueian
Keyword(s):  
Breast Cancer ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Therapeutic Effects ◽  
Mice Model ◽  
Fruit Extract ◽  
Phenolic Fraction ◽  
Juniperus Polycarpos

Abstract Background: This research was performed to synthesize nanophytosomes-loaded high phenolic fraction (HPF) from Juniperus polycarpos fruit extract and investigate its antiproliferation effects against breast cancer in mice model. Results: The nanophytosomes-loaded HPF from Juniperus polycarpos fruit extract was synthesized. The mice trial was conducted to determine the possible toxic effects of the synthesized nanophytosomes. The anticancer, pro-apoptotic, and antioxidative activities of the nanophytosomes were determined. The nanophytosomes-loaded HPF had a spherical structure with a size of 176 nm and a polydispersity index coefficient of 0.24. The in-vivo study manifested that nanophytosomes-loaded HPF significantly improved weight gain and food intake compared to the negative control group (p<0.05). The nanophytosomes-loaded HPF significantly enhanced the expression of bax (3.4-fold) and caspase-3 (2.7-fold) genes but reduced bcl2 (3.6-fold) gene expression in tumor cells. The average tumor size was significantly decreased in mice treated with nanophytosomes-loaded HPF (p<0.05). The expression of GPX (2.3-fold) and SOD (2.7-fold) antioxidants in the liver of mice supplemented with nanophytosomes-loaded HPF was significantly developed compared to the negative control (p<0.05). The nanophytosomes-loaded HPF did not show toxicity on normal cells. Conclusion: Our results indicated that nanophytosomes-loaded HPF might be a potential anticancer agent for the breast cancer treatment.

scholarly journals Efektivitas Ekstrak Artemisia vulgaris L. sebagai Hepatoprotektor pada sel-sel Hati Tikus yang Diinduksi Niasin

2018 ◽  
Vol 6 (2) ◽  
pp. 251
Author(s):  
Laily Rahmawati ◽  
Erma Sulistyaningsih ◽  
Rosita Dewi
Keyword(s):  
Treatment Group ◽  
Liver Damage ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Artemisia Vulgaris ◽  
Group I ◽  
Group Ii

  The niacin in energy drinks has metabolic product that cause oxidative stress and liver damage, while the liver damage can be prevented by hepatoprotective agents. Scoparone in Artemisia vulgaris L. can act as a hepatoprotector by its antioxidant effect. This study aimed to investigate the effectivity of Artemisia vulgaris L. extract as a hepatoprotector in wistar hepatocytes induced by niacin. This study used 25 male rats which were divided into 5 groups: normal, the negative control, the positive control, the treatment group I, and II. Treatment was conducted for 28 days. The samples were terminated and the hepatocyte were prepared for histological examination. Histological appearance was catagorized as mild, moderate, and severe damage with or without inflamatory cells activity. The data analysis by Kruskal Wallis showed significant difference (p<0,001). Further analysis by Mann Whitney revealed significantly difference (p<0,05) between normal group and all groups, negative control group and positive control group, and positive control group and treatment group I, but not significantly difference between negative control group and treatment group I, negative control group and treatment group II, positive control group and treatment group II, and between treatment groups. The study concluded that the effectivity of Artemisia vulgaris L. extract has not been proven as a hepatoprotector but further study is needed to draw a definite conclusion.   Keywords: energy drink, niacin, Artemisia vulgaris L., hepatoprotector  

scholarly journals Anti-nociceptive Potential of Ethanol Extract of Terminalia macroptera Guill&Perr (Combretaceae) Stem Bark in Mice

2021 ◽  
Vol 16 (2) ◽  
pp. 13-21
Author(s):  
S.A. Atunwa ◽  
M.O. Amali ◽  
S.O. Lawal ◽  
S.O. Usman ◽  
A.I. Olapade
Keyword(s):  
Acetic Acid ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Stem Bark ◽  
Negative Control ◽  
Flowering Plant ◽  
Control Group ◽  
Group I ◽  
Tail Immersion ◽  
Dose Dependent

Background: Terminalia macroptera Guill. &Perr. (Combretaceae) is a flowering plant with several ethno-medicinal claims. However, the dearth of information on its analgesic property has necessitated this study.Objectives: to evaluate the anti-nociceptive potential of ethanol extract of Terminalia macroptera stem bark (TMSB) in mice.Materials and Methods: Male and female mice of weight range 22 – 25g were randomly allotted into seven groups (n= 5) and treated as follows: Group I received 0.5 mL distilled water orally (negative control), Groups II-V were orally administered ethanol extract of T. macroptera stem bark (TMSB) at 50, 100, 200, and 400 mg/kg respectively while groups VI-VII received piroxicam 10 mg/kg and pentazocine 2 mg/kg intraperitoneally respectively as standards. The same treatment pattern was adopted for both pain models: tail immersion and acetic acid-induced writhing assays. Data were expressed as mean ± standard error of mean (SEM) using two-way analysis of variance (ANOVA) followed by Tukey’s and Bonferroni's multiple comparisons tests with p < 0.05 taken as significance.Results: The ethanolic extract of Terminalia macroptera stem bark showed significant dose-dependent anti-nociceptive activity at 100 and 400 mg/kg (2.95±0.41 and 2.9±0.31 respectively) 60 min post-treatment compared to the negative control group in the tail immersion test. Significant inhibition of nociception (0.20±0.20) was obtained at 400 mg/kg compared to the negative control group in the acetic acid-induced writhing test.Conclusions: The ethanol extract of Terminalia macroptera stem bark exhibited dose-dependent anti-nociceptive potential in both tail immersion and acetic acid-induced writhing assays in mice.

scholarly journals Protection Effectiveness Of Bit (Beta vulgaris) Fruit Extract In Maintaining Spermatogenesis Ability In White Rats (Rattus novergicus) Induced Carbon Tetracloride (CCl4)

2021 ◽  
Vol 10 (2) ◽  
pp. 36
Author(s):  
Alvyan Lantang Anugrah ◽  
Hana Eliyani ◽  
Budi Utomo ◽  
Suherni Susilowati ◽  
Maslichah Mafruchati ◽  
...  
Keyword(s):  
Beta Vulgaris ◽  
Sertoli Cells ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
Root Extract ◽  
Beet Root ◽  
White Rats

The aim of this research was to know whether beetroot (Beta Vulgaris) extract could protect spermatogenesis by maintaining spermatogenic and sertoli cell count  in rats (Rattus novergicus) induced with CCl4. Rats were given beetroot extract daily, for 14 days and 3ml/kg BW CCl4 intraperitoneally one hour after last treatment. This study used twenty rats which were devided equally into 5 groups. K(-), the negative control group was not induced with CCL4 and only given 1% CMC-Na suspension. K(+), the positive control group was induced with CCl­4 and given 1% CMC-Na suspension. P1, P2 and P3 were given beet root extract with doses of 200, 400, and 800 mg/kg BW daily before feeding. All of the beetroot treatment were given orally (2 ml). After 24 hours CCl4 induction, rats were sacrificed and testis were collected to make histology slides. The observations showed significantly different (p<0,05) in all of variables. Result showed significant differences in spermatogenic and sertoli cells between K(-) and K(+) groups, K(+) with P2 and P3 group, and showed insignificant difference between P2 and P3 group in spermatogenic and sertoli cells. The result of this research showed that beetroot extract could protect the spermatogenic and sertoli cells in male rats induced with CCl4.

TOTAL FLAVONOID DAN EFEKTIVITAS EKSTRAK ETANOL BIJI KELOR (Moringa oleifera L) ASAL KOTA PALU SULAWESI TENGAH TERHADAP HISTOPATOLOGI PANKREAS TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI STREPTOZOTOCIN

2020 ◽  
Vol 6 (1) ◽  
pp. 24
Author(s):  
Viani Anggi ◽  
Joni Tandi ◽  
Veronika Veronika
Keyword(s):  
Ethanol Extract ◽  
Negative Control ◽  
Total Flavonoid ◽  
Male Rats ◽  
Control Group ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
White Rats ◽  
Group I ◽  
Streptozotocin Induced Diabetes

This study aims to determine the content of flavonoid and the effect of ethanol extract of moringa seeds on the regeneration of pancreatic β cells in male white rats streptozotocin induced diabetes. This study method used has total flavonoid equivalent quercetin by spectrophotometry uv-vis and to regeneration of pancreatic β cells in male white rats used 30 test animals,namely male white rats divided into 6 groups, each group consisted of 5 male white rats with details of group I as normal control, Group II as negative control given 0.5% Na-CMC suspension, Group III as positive control given glibenclamide suspension and in Groups IV, V, and VI were given with each dose of 100 mg/kg BW, 200 mg/kg BW and 400 mg/kg BB. Histopathological damage picture of the pancreas was observed by staining HE using a 400x magnification olympus Cx21 microscope. The results showed that the ethanol extract of moringa seeds contained secondary metabolites, namely flavonoids, alkaloids, saponins and tannins. The results showed has total flavonoid equivalent quercetin of moringa seeds is 1,26% and regeneration of pancreatic β cells in male white rats streptozotocin induced diabetes of Moringa seed ethanol extract at a dose of 400 mg/kg BB can have an effect on the regeneration of β cells in the pancreas of white diabetic male rats.  

The Effect of Ethanol Extract of Guava Leaf on Hypercholesterolemia-Diabetes of White Rats Male Induced by High Fat Feed and Streptozotocin

2021 ◽  
Vol 7 (2) ◽  
pp. 177-187
Author(s):  
Joni Tandi ◽  
Franciska Diana Pratiwi ◽  
Surisna Surisna ◽  
Niluh Puspita Dewi ◽  
Magfirah Magfirah
Keyword(s):  
Blood Glucose ◽  
Effective Dose ◽  
Ethanol Extract ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels ◽  
Group I ◽  
Guava Leaf

This study aims to determine the effect of guava leaf extract (Psidiium guajava L) on hypercholesterolemia-diabetes and the effective dose for reducing cholesterol and blood glucose levels. This study is a laboratory experimental study using 30 rats divided into 6 test animals. the treatment group, each group consisted of 5 rats. Group I was normal control, group II negative control was given Na-CMC suspension, group III positive control was given simvastatin group IV dose 150 mg / kg BW, group V dose 250 mg / kg BW and group VI dose 350 mg / kg BW. The results showed that the ethanol extract of guava leaves had an effect on reducing blood glucose levels of male white rats with an effective dose of 250mg / kg BW with an average reduction of 119 mg/dL and effectively reduce cholesterol levels at a dose of 150 mg /kg BW with an average reduction of 28,33 mg/dL.

scholarly journals ASSESSMENT OF ANTI-INFLAMMATORY ACTIVITY OF ETHANOLIC EXTRACT OF ASAM KANDIS (GARCINIA XANTHOCHYMUS HOOK. F. EX T. ANDERSON) FRUIT

2018 ◽  
Vol 11 (4) ◽  
pp. 81
Author(s):  
Hanafis Sastra Winata ◽  
Rosidah Rosidah ◽  
Panal Sitorus
Keyword(s):  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Inflammatory Activity ◽  
Control Group ◽  
Phytochemical Screening ◽  
Fruit Extract ◽  
Garcinia Xanthochymus ◽  
Anti Inflammatory

 Objective: The objective of this study was to evaluate the anti-inflammatory activity in acute and subacute models of inflammation from ethanolic fruit extract of Asam kandis (Garcinia xanthochymus Hook. f. ex T. Anderson) in animal (rats) models.Methods: Pleliminary phytochemical screening was carried out by using standard procedures.. Assessment of acute and subacute models of inflammation was using carrageenan-induced paw edema method and cotton pellet granuloma method using three dosage treatments; 200 mg/kg BW, 400 mg/kg BW, and 800 mg/kg BW along with a negative control group (0.5% Na CMC) and positive control (Na diclofenac 2.25 mg/kg BW). The inhibition period was observed at 30, 60, 90, 120, 150, and 180 min time intervals.Result: The phytochemical screening showed that the ethanolic fruit extract from Asam kandis contain contains flavonoids, glycosides, steroids, and triterpenoids. The anti-inflammatory result showed that the strongest inhibition produced by ethanolic fruit extract of Asam kandis occurred on the dosage of 800 mg/kg BW compared to the other doses (200 and 400 mg/kg BW) throughout the observation period.Conclusion: This finding indicated that ethanolic fruit extract of Asam kandis (G. xanthochymus Hook. f. ex T. Anderson) might become an interesting candidate for treatment of inflammation.

scholarly journals Comparative Evaluation of Apical Sealing Ability of Different Obturation Techniques by Confocal Microscopy: An In Vitro Study

2020 ◽  
Vol 8 (02) ◽  
pp. 55-59
Author(s):  
Reetu Arora ◽  
Yogesh Kumar ◽  
Neetu Jindal ◽  
Renu Aggarwal ◽  
Kavneet Takhar
Keyword(s):  
Root Canal ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Group Iv ◽  
Control Group ◽  
Group Vi ◽  
Group V ◽  
Group Iii ◽  
Group I

Abstract Introduction The aim of obturation in the root canal is to completely seal the canal space to eliminate all the portals of entry and exit between root canal and periodontal space. Various techniques have been developed to achieve a hermetic seal. Materials and Methods As many as 150 extracted human maxillary central incisors were taken for the study. Biomechanical preparation was done up to F5 protaper file. According to different obturation techniques, samples were divided into six groups, keeping 30 samples in experimental and 15 samples in control groups. Group I–Lateral Condensation, Group II–Thermafil, Group III–Beefill, Group IV–GuttaFlow, Group V–Positive Control group, Group VI–Negative Control group. After obturation, the samples were immersed in 2% Rhodamine-B dye for 24 hours. Each sample was longitudinally sectioned to examine under confocal laser scanning microscope. Statistical Analysis The results were evaluated with ANOVA and posthoc Tukey honest significant difference (HSD) comparison test. Results The mean values of dye penetration of different groups were Group I (Lateral Condensation) 1.51 ± 0.451, Group II (Thermafil) 0.918 ± 0.399, Group III (Beefill) 1.30 ± 0.559. Group IV (GuttaFlow) 0.655 ± 0.396, Group V (Positive Control group) 1.96 ±0.046, Group VI (Negative Control group) 0 ± 0. The lowest mean value of apical microleakage was found in GuttaFlow amongst all experimental groups. Conclusion It can be concluded that the GuttaFlow obturating material exhibited better apical sealing ability with canal walls.

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