scholarly journals Biological activities of the liverwort Marchantia polymorpha L. collected at Da Lat, Lam Dong province

2019 ◽  
Vol 2 (5) ◽  
pp. 26-34
Author(s):  
Tan Quoc Tran ◽  
Tam Thien Luong ◽  
Hoang Ngo Phan ◽  
Phuong Ngo Diem Quach
Keyword(s):  
Ethyl Acetate ◽  
Biological Activities ◽  
Folk Medicine ◽  
Secondary Compounds ◽  
Chloroform Extract ◽  
Marchantia Polymorpha ◽  
Traditional Treatment ◽  
Potential Applications ◽  
Contemporary Medicine ◽  
Mcf 7

Marchantia polymorpha L. is used as a folk medicine in India, China and some European countries for the treatment of inflammation, cure cuts, wounds, diuretics, etc. Natural compounds and biological activities of this liverwort have been studied in the world for few years. At present time, the studies on bryophytes (non-vascular plants) are quite limited, especially in Vietnam. Therefore, this research focused on investigating some biological activities and analyzing of major secondary metabolites of M. polymorpha L. collected at Da Lat, Lam Dong. Among four extracted fractions (nhexane, chloroform, ethyl acetate and ethanol), the chloroform extract exhibited the best antioxidant, antibacterial, anti-proliferation of MCF-7 cell line activities. The ethyl acetate extract exhibited better activity of tyrosinase and α-glucosidase inhibition than others. Main secondary compounds in this species were phenolics, steroids and glycosidederivatives. These results were scientific evidences that might help us to understand rigorously about the ultilization of Marchantia liverwort in the traditional treatment and new potential applications in the contemporary medicine.

scholarly journals Biological activities of in vitro liverwort Marchantia polymorpha L. extracts

2020 ◽  
Vol 48 (2) ◽  
pp. 826-838
Author(s):  
Tan Q. TRAN ◽  
Hoang N. PHAN ◽  
Anh L. BUI ◽  
Phuong N. D. QUACH
Keyword(s):  
Ethyl Acetate ◽  
Iron Reduction ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Marchantia Polymorpha ◽  
Coarse Powder ◽  
Constant Weight ◽  
Mcf 7

To overcome the problems in liverwort collecting such as small size and easily mixed with other species in the wild, we have successfully cultivated Marchantia polymorpha L. under in vitro conditions in the previous study. The aim of this study is to evaluate the biological activities of this in vitro biomass as a confirmation of the sufficient protocol in cultivation this species. Cultured biomass was dried at a temperature of 45-50 oC to constant weight and ground into a fine powder. The coarse powder was extracted with organic solvents of increasing polarization including n-hexane, chloroform, ethyl acetate, and ethanol using the maceration technique. Four extracts were investigated antioxidant (iron reduction power, DPPH), antibacterial (agar diffusion), tyrosinase inhibitory activity, anti-proliferation on MCF-7 cells. Additionally, the presence of natural metabolite groups of the extracts was detected by using specific reagents. For antioxidant activity, ethyl acetate fraction extract had the highest iron reducing power and DPPH free radical scavenging ability with IC50 = 439.31 µg ml-1. All three n-hexane, chloroform, and ethyl acetate extracts possessed resistance to the bacterial strain tested. At a concentration of 2 mg ml-1, n-hexane and chloroform extracts had the highest percentage of tyrosinase inhibition (69.54 and 69.10%, respectively). The n-hexane extract is a potent extract that inhibits the proliferation of MCF-7 cells with the lowest IC50 of 38.15 µg ml-1. A preliminary chemical composition survey showed that the cultured biomass liverwort contains many bioactive compounds, particularly the compounds of range of non- and less-polarized fractions.

scholarly journals Chemical Constituents of Indonesian Micromelum minutum Leaves and Their Cytotoxicity Against MCF-7 and 4T1 Breast Cancer Cells

2021 ◽  
Vol 53 (1) ◽  
pp. 97-108
Author(s):  
Ratna Asmah Susidarti ◽  
Edy Meiyanto ◽  
Muthi' Ikawati ◽  
Normaidah Normaidah ◽  
Nurramadhani Armada Sida
Keyword(s):  
Ethyl Acetate ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Folk Medicine ◽  
Mtt Method ◽  
South Sulawesi ◽  
4T1 Cells ◽  
4T1 Breast Cancer ◽  
Mcf 7 ◽  
Ethyl Acetate Extracts

Micromelum minutum is used widely in traditional folk medicine. Although this species has been investigated extensively and several bioactive compounds have been isolated, little work has been done on Indonesian M. minutum. This research aimed to study the chemical constituents and biological activities of M. minutum cultivated in Bantimurung Bulusaraung National Park, South Sulawesi, Indonesia. The isolated compounds were assessed for their cytotoxicity towards MCF-7 and 4T1 cell lines by MTT method. The dried ground leaves of M. minutum were sequentially macerated with n-hexane, ethyl acetate, and methanol. The n-hexane and ethyl acetate extracts contained a flavonoid 5,7-dihydroxy-3,4',8-trimethoxyflavone (1) which inhibited MCF-7 and 4T1 cell viability by 50% at concentrations of 369±8 and 227±5 µM, respectively. Further separation of the ethyl acetate extract by column chromatography yielded acetyldihydromicromelin A (2) and a mixture of dihydromicromelin A (3) and dihydromicromelin B (4), which were not active toward MCF-7 and 4T1 cells.

scholarly journals Chemical Constituents of Indonesian Micromelum minutum Leaves and Their Cytotoxicity Against MCF-7 and 4T1 Breast Cancer Cells

2021 ◽  
Vol 53 (1) ◽  
pp. 97-108
Author(s):  
Ratna Asmah Susidarti ◽  
Edy Meiyanto ◽  
Muthi Ikawati ◽  
Normaidah Normaidah ◽  
Nurramadhani A. Sida
Keyword(s):  
Ethyl Acetate ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Folk Medicine ◽  
Mtt Method ◽  
South Sulawesi ◽  
4T1 Cells ◽  
4T1 Breast Cancer ◽  
Mcf 7 ◽  
Ethyl Acetate Extracts

Micromelum minutum is used widely in traditional folk medicine. Although this species has been investigated extensively and several bioactive compounds have been isolated, little work has been done on Indonesian M. minutum. This research aimed to study the chemical constituents and biological activities of M. minutum cultivated in Bantimurung Bulusaraung National Park, South Sulawesi, Indonesia. The isolated compounds were assessed for their cytotoxicity towards MCF-7 and 4T1 cell lines by MTT method. The dried ground leaves of M. minutum were sequentially macerated with n-hexane, ethyl acetate, and methanol. The n-hexane and ethyl acetate extracts contained a flavonoid 5,7-dihydroxy-3,4',8-trimethoxyflavone (1) which inhibited MCF-7 and 4T1 cell viability by 50% at concentrations of 369±8 and 227±5 µM, respectively. Further separation of the ethyl acetate extract by column chromatography yielded acetyldihydromicromelin A (2) and a mixture of dihydromicromelin A (3) and dihydromicromelin B (4), which were not active toward MCF-7 and 4T1 cells.

scholarly journals Total Phenolics, Flavonoids, Condensed Tannins Content of Eight Centaurea Species and Their Broad Inhibitory Activities against Cholinesterase, Tyrosinase, α-Amylase and α-Glucosidase

2016 ◽  
Vol 44 (1) ◽  
pp. 195-200 ◽  
Author(s):  
Gokhan ZENGIN ◽  
Marcello LOCATELLI ◽  
Simone CARRADORI ◽  
Andrei M. MOCAN ◽  
Abdurrahman AKTUMSEK
Keyword(s):  
Ethyl Acetate ◽  
Condensed Tannins ◽  
Total Phenolics ◽  
Condensed Tannin ◽  
Folk Medicine ◽  
Chloroform Extract ◽  
New Drugs ◽  
Inhibitory Effects ◽  
Synthetic Drugs ◽  
Starting Point

Several bioactive compounds originate from natural sources and their uses are generally related with traditional or folk medicine. Synthetic drugs can have adverse side effects and, for this reason, the investigation of novel, safe, and natural-occurring products can account for the development of new drugs. The genus Centaurea L. is one of the most important genera of the Asteraceae family, containing more than 200 species in the Turkish flora, about 140 of which are endemic. The aim of the present work was to determine enzyme inhibitory potentials of two extracts (chloroform and ethyl acetate) from eight Centaurea species against cholinesterase, tyrosinase, amylase, and glucosidase. The total phenolics, flavonoids and condensed tannin contents were also reported for each extract. These components in the extracts varied according to species and extraction solvents as well as enzyme inhibitory effects. The highest level of phenolics was found to be in the chloroform extract of C. pulchella (119.23 mg GAEs/g extract). Generally, chloroform extracts exhibited stronger enzyme inhibitory effects as compared to ethyl acetate. Additionally, possible correlations with total phenolics, flavonoids, and condensed tannins content were also highlighted. This paper is the first report of the inhibitory capacities of the eight Centaurea species on the selected enzymes. The present results may be a valuable starting point in the development of new bioactive formulations.

scholarly journals Cyperus rotundus cyperaceae: a study of phytochemistry, total polyphenol content, flavonoid content, and antioxidant activity

2021 ◽  
Vol 332 ◽  
pp. 06003
Author(s):  
Quy Nguyen Ngoc ◽  
Tien Nguyen Minh
Keyword(s):  
Antioxidant Activity ◽  
Folk Medicine ◽  
Secondary Compounds ◽  
Cyperus Rotundus ◽  
Aqueous Extracts ◽  
Flavonoid Content ◽  
Total Polyphenol ◽  
Anti Cancer ◽  
Potential Applications ◽  
The Rich

Cyperus rotundus is a folk medicine with many benefits such as anti-inflammatory, anti-cancer, antioxidant, etc. It is widely used in many parts of the world. This study provided data on the phytochemical composition of Cyperus rotundus rhizome, including the active groups of alkaloids, flavonoids, phenols, tannins, and triterpenoids. The polyphenols content in the ethanolic and aqueous extracts of Cyperus rotundus were 26.89 ± 4.33 (mgGAE/g) and 51.84 ± 6.46 (mgGAE/g), respectively. While, the flavonoids content in the ethanolic and aqueous extracts of Cyperus rotundus were 78.03 ± 3.11 (mgQE/g) and 20.28 ± 2.25 (mgQE/g), respectively. According to the ABTS assay, the IC50 values of the aqueous and ethanolic extracts were 117.8 ± 19.85 (μg/ml) and 162.02 ± 11.43 (μg/ml), respectively. According to the DPPH assay, the IC50 values of Cyperus rotundus extracted by water and alcohol were 337.42 ± 22.84 (μg/ml) and 447.53 ± 33.8 (μg/ml), respectively. With the rich presence of secondary compounds, antioxidant activity confirmed in many studies, Cyperus rotundus is evaluated as having potential applications in many different fields such as pharmaceuticals, cosmetics, and functional foods.

Vitex Diterpenoids: Structural Diversity and Pharmacological Activity

2020 ◽  
Vol 26 (1) ◽  
pp. 138-159 ◽  
Author(s):  
Yanfei Ban ◽  
Tianshuang Xia ◽  
Rui Jing ◽  
Yaoli Guo ◽  
Yiya Geng ◽  
...  
Keyword(s):  
Pharmacological Activity ◽  
Hormone Level ◽  
Biological Activities ◽  
Folk Medicine ◽  
Structural Diversity ◽  
Pharmacological Activities ◽  
Structure Activity ◽  
Wide Range ◽  
Potential Applications

Plants of the genus Vitex (Verbenaceae) are mainly distributed throughout tropical and temperate regions, and many Vitex plants have been traditionally used in folk medicine. Plants of this genus are a rich source of diterpenoids, which not only displayed versatile structural diversity with potential chemotaxonomical significance but also exhibited a wide range of biological activities, mainly including in vitro cytotoxic, antiinflammatory, antimicrobial, hormone level-regulating and antiangiogenic activities. Recently, a series of bioactive diterpenoids, with interesting carbon skeletons, have been reported and gathered considerable interest. This article systematically reviewed diterpenoids isolated from the genus Vitex that appeared in the literature up to December 2018, critically highlighting their structural diversity and pharmacological activities. Up to now, a total of 154 diterpenoids with diverse structures have been isolated and identified from Vitex plants. The authors also summarized the reported structure-activity relationships of those well explored Vitex diterpenoids. Finally, the authors discussed the challenges and potential applications of these diterpenoids in the future.

scholarly journals ANTI-PROLIFERATIVE POTENTIAL OF Carica papaya LEAVES ON BREAST CANCER CELLS – MCF-7

2021 ◽  
Vol 9 (5) ◽  
pp. 678-686
Author(s):  
Sasikala Mariyappan ◽  
◽  
Sivakumar Ramalingam ◽  
Lavanya Murugan ◽  
Renuka Saravanan ◽  
...  
Keyword(s):  
Free Radical ◽  
Ethyl Acetate ◽  
Dna Fragmentation ◽  
Carica Papaya ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Free Radical Scavenging ◽  
Phytochemical Analysis ◽  
Proliferative Effect ◽  
Mcf 7

The study's objective is to identify the phytoconstituents and determine the anti-cancer potential of Carica papaya leaves against the MCF 7 cell line. Chloroform, ethyl acetate, and methanol extracts of C. papaya leaves were prepared by cold maceration method and qualitative phytochemical analysis was performed. The anti-proliferative effect of these extracts was determined by 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and apoptotic assay by acridine orange/ethidium bromide staining method on MCF 7 cells. The effect of the extracts, with different concentrations, on DNA fragmentation, was also performed on MCF 7 cells. Qualitative analysis revealed the presence of alkaloids, flavonoids, terpenoids, steroids, saponins, tannins, glycosides, phenols, anthraquinones, proteins, and carbohydrates. Chloroform, methanol, and ethyl acetate extracts of C. papaya leaves were observed with potential DPPH free radical scavenging activity with 72%, 75%, and 78% respectively. Of these extracts, the chloroform extract (72%) was found to possess a more free radical scavenging effect against DPPH and also showed a dose-dependent effect, the maximum at 100µg/ml, on DNA fragmentation in MCF 7 cells. Further, chloroform extract showed a maximum anti-proliferative effect on MCF-7 cells with IC50 at 22±1.5µg/ml, whereas methanol and ethyl acetate extract at 30±0.5 µg/ml and 28±0.5 µg/ml respectively. Increased apoptosis in MCF 7 cells was observed with an increased concentration of chloroform extract of C. papaya. From the results of this study, it can be concluded that leaf extract of C. papaya found to possess an anti-proliferative effect and antioxidant potential and it could be due to the presence of rich secondary metabolites of the plant.

scholarly journals In Vitro Assessment of Antiplasmodial and Antitrypanosomal Activities of Chloroform, Ethyl Acetate and Ethanol Leaf Extracts of Oedera genistifolia

2020 ◽  
Vol 10 (19) ◽  
pp. 6987
Author(s):  
Kunle Okaiyeto ◽  
Anthony I. Okoh
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Hela Cells ◽  
Biological Activities ◽  
Ethanol Extract ◽  
Chloroform Extract ◽  
Leaf Extracts ◽  
Resistance Evolution ◽  
Crude Extracts

The high resistance evolution of protozoans to the existing antiparasitic drugs has necessitated the quest for novel and effective drugs against plasmodium and trypanosome parasites. As a result, this study aimed to assess the antiplasmodial and antitrypanosomal potentials of chloroform, ethyl acetate and ethanol leaf extracts of Oedera genistifolia. Standard biochemical procedures were explored for the plant extraction and gas chromatography-mass spectroscopy (GCMS) was used to identify the bioactive compounds in the crude extracts. The cytotoxic effects of the crude extracts were assessed against human cervix adenocarcinoma (HeLa cells) and their antiparasitic activities were investigated against Plasmodium falciparum strain 3D7 and Trypanosoma brucei brucei. GCMS analyses of the crude extracts revealed the bioactive compounds that could be responsible for the biological activities. The extracts had no cytotoxic effect on HeLa cells and demonstrated good antiplasmodial activity (chloroform extract: IC50 = 11.6 µg∙mL−1, ethyl acetate extract: IC50 = 3.3 µg∙mL−1 and ethanol extract: IC50 = 3.7 µg∙mL−1). Likewise, they showed excellent antitrypanosomal activity with IC50 = 0.5 µg∙mL−1 for chloroform and ethyl acetate extracts and IC50 = 0.4 µg∙mL−1 for the ethanol extract. Findings from the present study indicated that O. genistifolia could be a good source of strong antiplasmodial and antitrypanosomal agents.

EVALUATION OF DIFFERENT BIOLOGICAL ACTIVITIES OF LEAVES OF EHRETIA ACUMINATA R. BR.

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (4) ◽  
pp. 42-49
Author(s):  
Abha Shukla ◽  
◽  
Amanpreet Kaur ◽  
Rishi K. Shukla ◽  
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Biological Activities ◽  
Food Poisoning ◽  
Value Added ◽  
Ethanol Extract ◽  
Chloroform Extract ◽  
Antidiabetic Activity ◽  
Biological Potential ◽  
Medicinal Properties

Ehretia acuminata R. Br. is a plant with alternative medicinal properties, commonly found in Asian, African and North American countries. The present study was undertaken to prepare crude extracts of E. acuminata leaves with different polarity solvents (petroleum ether (PE), chloroform (CH), ethyl acetate (EA), ethanol (EOL) and aqua (AQ)) by using successive Soxhelet extraction method and assessing antidiabetic, antiinflammatory and antihemolytic activities by the spectrophotometric method and antimicrobial activity by the zone inhibition method. The highest antidiabetic activity was found in the chloroform extract (IC50 260-265 µg/mL) and the lowest was in the aqueous extract (IC50 1560-1550 µg/mL). In anti-inflammatory assay, highest potential was shown by ethyl acetate (IC50 290 µg/mL) extract and lowest in petroleum ether (IC50 750µg/mL) extract. The zone of inhibition is highest in ethanol extract (12-18mm) by different food poisoning microbes, similarly, ethyl acetate extract showed the highest potential in antihemolytic activity with IC50 90 µg/mL. Many extracts of E. acuminata leaves showed a high biological potential that could be attributed to the high amount of phytoconstituents and thus could be utilized as value added functional food.

scholarly journals Anti-Breast Cancer Potency of Multistage Extraction from Jamur Dewa (Agaricus blazei Murill) Solvents on MCF-7 Cells

2017 ◽  
Vol 8 (2) ◽  
pp. 68
Author(s):  
Misgiati Misgiati ◽  
Sukardiman Sukardiman ◽  
Aty Widyawaruyanti
Keyword(s):  
Breast Cancer ◽  
Ethyl Acetate ◽  
Water Extract ◽  
Extraction Process ◽  
Chloroform Extract ◽  
Agaricus Blazei ◽  
Butanol Extract ◽  
Mcf 7 ◽  
Agaricus Blazei Murill

ABM (Agaricus blazei Murill) is a basidiomycetes fungus. ABM is used by people for the treatment of diabetes, antihypertention, anticholesterol, anticancer, and immunostimulant. ABM contains terpene, steroids, agaritine, vitamin C, vitamin E, and betaglucane. In this research, ABM extract was tested as an anti-breast cancer in vitro using MCF-7 breast cancer cells. The extract was obtained from the multistage extraction process of several solvents in turn, the solvent used, among others, n-hexane, dichloromethane (DCM), chloroform, ethyl acetate, butanol, and water. The results of the research were the obtained IC50 value from n-hexane extract 247,17 μg /ml; extract DCM 227μg/ml ; chloroform extract 215,64 μg /ml ; extract of ethyl acetate 234,9 μg/ml ; butanol extract 500,78 μg/ml; while the water extract was inactive. Based on these results can be considered for further research to fractionate in order to know which class compounds have the potency as anticancer within the extracts.Key words : Agaricus blazei, multistage extraction, MCF-7 cells.

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