scholarly journals Evaluation of two Pseudomonas strains isolated from a maize rhizosphere as a plant growth promoting rhizobacteria

2019 ◽  
Vol 2 (2) ◽  
pp. 38-46
Author(s):  
Thanh Nguyen Chu ◽  
Nhi Yen Nguyen ◽  
Diep Ngoc Dao ◽  
Bao Thi Hoai Tran ◽  
Minh Thi Thanh Hoang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Diseases ◽  
In Vitro Tests ◽  
Maize Rhizosphere ◽  
Plant Growth Promoting ◽  
Growth Promoting

Plant growth promoting rhizobacteria (PGPR) are free-living soil bacteria (rhizosphere bacteria), rhizoplane bacteria or endophytic bacteria that may promote plant growth and suppress plant diseases. The aim of this study is to evaluate effects of 2 rhizobacteria strains belonging to the genus Pseudomonas isolated from maize rhizosphere on the plant growth promotion. The in vitro tests showed that both of strain could fix nitrogen, solubilize phosphate, produced phytohormones (IAA and GAs), and improved the germination and growth of Arabidopsis thaliana. Under the greenhouse condition, growth parameters of bacteria inoculated maizes (fresh weight of shoot, dry weight of root, chlorophyll content) were also increased significantly than those of uninoculated ones. Our results reported 2 promising bacteria strain candidates and revealed their potential as a biological agent for eco-friendly agricultural practices.

scholarly journals Plant Growth Promoting Rhizobacteria, Arbuscular Mycorrhizal Fungi and Their Synergistic Interactions to Counteract the Negative Effects of Saline Soil on Agriculture: Key Macromolecules and Mechanisms

2021 ◽  
Vol 9 (7) ◽  
pp. 1491
Author(s):  
Alka Sagar ◽  
Parikshita Rathore ◽  
Pramod W. Ramteke ◽  
Wusirika Ramakrishna ◽  
Munagala S. Reddy ◽  
...  
Keyword(s):  
Plant Growth ◽  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Acc Deaminase ◽  
Arbuscular Mycorrhizal ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting

Soil saltiness is a noteworthy issue as it results in loss of profitability and development of agrarian harvests and decline in soil health. Microorganisms associated with plants contribute to their growth promotion and salinity tolerance by employing a multitude of macromolecules and pathways. Plant growth promoting rhizobacteria (PGPR) have an immediate impact on improving profitability based on higher crop yield. Some PGPR produce 1-aminocyclopropane-1-carboxylic (ACC) deaminase (EC 4.1.99.4), which controls ethylene production by diverting ACC into α-ketobutyrate and ammonia. ACC deaminase enhances germination rate and growth parameters of root and shoot in different harvests with and without salt stress. Arbuscular mycorrhizal fungi (AMF) show a symbiotic relationship with plants, which helps in efficient uptake of mineral nutrients and water by the plants and also provide protection to the plants against pathogens and various abiotic stresses. The dual inoculation of PGPR and AMF enhances nutrient uptake and productivity of several crops compared to a single inoculation in both normal and stressed environments. Positively interacting PGPR + AMF combination is an efficient and cost-effective recipe for improving plant tolerance against salinity stress, which can be an extremely useful approach for sustainable agriculture.

scholarly journals Selection and Assessment of Plant Growth-Promoting Rhizobacteria for Biological Control of Multiple Plant Diseases

2017 ◽  
Vol 107 (8) ◽  
pp. 928-936 ◽  
Author(s):  
Ke Liu ◽  
Molli Newman ◽  
John A. McInroy ◽  
Chia-Hui Hu ◽  
Joseph W. Kloepper
Keyword(s):  
Biological Control ◽  
Plant Growth ◽  
Broad Spectrum ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Diseases ◽  
Damping Off ◽  
In Planta ◽  
Plant Growth Promoting ◽  
Growth Promoting

A study was designed to screen individual strains of plant growth-promoting rhizobacteria (PGPR) for broad-spectrum disease suppression in vitro and in planta. In a preliminary screen, 28 of 196 strains inhibited eight different tested pathogens in vitro. In a secondary screen, these 28 strains showed broad spectrum antagonistic activity to six different genera of pathogens, and 24 of the 28 strains produced five traits reported to be related to plant growth promotion, including nitrogen fixation, phosphate solubilization, indole-3-acetic acid production, siderophore production, and biofilm formation. In advanced screens, the 28 PGPR strains selected in vitro were tested in planta for biological control of multiple plant diseases including bacterial spot of tomato caused by Xanthomonas axonopodis pv. vesicatoria, bacterial speck of tomato caused by Pseudomonas syringae pv. tomato, damping-off of pepper caused by Rhizoctonia solani, and damping-off of cucumber caused by Pythium ultimum. In all, 5 of the 28 tested strains significantly reduced three of the four tested diseases, and another 19 strains showed biological control to two tested diseases. To understand the observed broad-spectrum biocontrol capacity, antiSMASH was used to predict secondary metabolite clusters of selected strains. Multiple gene clusters encoding for secondary metabolites, e.g., bacillibactin, bacilysin, and microcin, were detected in each strain. In conclusion, selected individual PGPR strains showed broad-spectrum biocontrol activity to multiple plant diseases.

scholarly journals Isolation and identification of plant growth promoting rhizobacteria from maize (Zea mays L.) rhizosphere and their plant growth promoting effect on rice (Oryza sativa L.)

2017 ◽  
Vol 57 (2) ◽  
pp. 144-151 ◽  
Author(s):  
Arun Karnwal
Keyword(s):  
Zea Mays ◽  
Plant Growth ◽  
Growth Promotion ◽  
Oryza Sativa L ◽  
Plant Growth Promoting Rhizobacteria ◽  
Promoting Effect ◽  
Isolation And Identification ◽  
Plant Growth Promoting ◽  
Growth Promoting

AbstractThe use of plant growth promoting rhizobacteria is increasing in agriculture and gives an appealing manner to replace chemical fertilizers, pesticides, and dietary supplements. The objective of our research was to access the plant growth promotion traits ofPseudomonas aeruginosa,P. fluorescensandBacillus subtilisisolated from the maize (Zea maysL.) rhizosphere.In vitrostudies showed that isolates have the potential to produce indole acetic acid (IAA), hydrogen cyanide, phosphate solubilisation, and siderophore. RNA analysis revealed that two isolates were 97% identical toP. aeruginosastrain DSM 50071 andP. aeruginosastrain NBRC 12689 (AK20 and AK31), while two others were 98% identical toP. fluorescensstrain ATCC 13525,P. fluorescensstrain IAM 12022 (AK18 and AK45) and one other was 99% identical toB. subtilisstrain NCDO 1769 (AK38). Our gnotobiotic study showed significant differences in plant growth variables under control and inoculated conditions. In the present research, it was observed that the isolated strains had good plant growth promoting effects on rice.

scholarly journals Rhizobacteria Associated with a Native Solanaceae Promote Plant Growth and Decrease the Effects of Fusariumoxysporum in Tomato

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 579
Author(s):  
Carmen Sanjuana Delgado-Ramírez ◽  
Rufina Hernández-Martínez ◽  
Edgardo Sepúlveda
Keyword(s):  
Plant Growth ◽  
Fusarium Wilt ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Positive Effects ◽  
Alternative Approach ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting

Plant growth-promoting rhizobacteria are often utilized to improve crop health and productivity. Nevertheless, their positive effects can be hindered if they fail to withstand the environmental and ecological conditions of the regions where they are applied. An alternative approach to circumvent this problem is a tailored selection of bacteria for specific agricultural systems. In this work, we evaluated the plant growth promoting and pathogen inhibition activity of rhizobacteria obtained from the rhizosphere of Mariola (Solanum hindsianum), an endemic shrub from Baja California. Eight strains were capable of inhibiting Fusarium oxysporum in vitro, and thirteen strains were found to possess three or more plant-growth-promotion traits. Molecular identification of these strains, using 16 s rRNA partial sequences, identified them as belonging to the genera Arthrobacter, Bacillus, Paenibacillus, Pseudomonas, and Streptomyces. Finally, the effect of selected plant growth-promoting rhizobacteria (PGPR) strains on the growth and suppression of Fusarium wilt in tomato was evaluated. Results showed that these strains improved tomato plants growth under greenhouse conditions and reduced Fusarium wilt effects, as reflected in several variables such as length and weight of roots and stem. This work highlights the potential of native plants related to regionally important crops as a valuable source of beneficial bacteria.

scholarly journals Role of Plant Growth-Promoting Rhizobacteria (PGPR) and Bio-Control Agents (BCAs) in Crop Production

2020 ◽  
Vol 7 (3) ◽  
pp. 144-150
Author(s):  
Sujata Kumari ◽  
◽  
Narender K. Bharat ◽  
Ashok K. Thakur ◽  
◽  
...  
Keyword(s):  
Plant Growth ◽  
Seed Treatment ◽  
Crop Production ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Diseases ◽  
Environment Friendly ◽  
Plant Growth Promoting ◽  
Growth Promoting

The bioagents like Plant Growth Promoting Rhizobacteria (PGPR) and Biocontrol Agents (BCAs) play a crucial role in plant growth promotion, nutrient uptake and suppression of biotic and abiotic stresses. Different researchers have applied these bioagents by various means either through seed treatment or through soil application to prevent various plant diseases. Thus, these non-chemical environment friendly tools can be exploited to enhance crop production.

scholarly journals Mixtures of Plant-Growth-Promoting Rhizobacteria Enhance Biological Control of Multiple Plant Diseases and Plant-Growth Promotion in the Presence of Pathogens

Plant Disease ◽  
2018 ◽  
Vol 102 (1) ◽  
pp. 67-72 ◽  
Author(s):  
Ke Liu ◽  
John A. McInroy ◽  
Chia-Hui Hu ◽  
Joseph W. Kloepper
Keyword(s):  
Biological Control ◽  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Pythium Ultimum ◽  
Plant Diseases ◽  
Disease Suppression ◽  
Plant Growth Promoting ◽  
Growth Promoting

Several studies have shown that mixtures of plant-growth-promoting rhizobacteria (PGPR) could enhance biological control activity for multiple plant diseases through the mechanisms of induced systemic resistance or antagonism. Prior experiments showed that four individual PGPR strains—AP69 (Bacillus altitudinis), AP197 (B. velezensis), AP199 (B. velezensis), and AP298 (B. velezensis)—had broad-spectrum biocontrol activity via antagonism in growth chambers against two foliar bacterial pathogens (Xanthomonas axonopodis pv. vesicatoria and Pseudomonas syringae pv. tomato) and one of two tested soilborne fungal pathogens (Rhizoctonia solani and Pythium ultimum). Based on these findings, the overall hypothesis of this study was that a mixture of two individual PGPR strains would exhibit better overall biocontrol and plant-growth promotion than the individual PGPR strains. Two separate greenhouse experiments were conducted. In each experiment, two individual PGPR strains and their mixtures were tested for biological control of three different diseases and for plant-growth promotion in the presence of the pathogens. The results demonstrated that the two individual PGPR strains and their mixtures exhibited both biological control of multiple plant diseases and plant-growth promotion. Overall, the levels of disease suppression and growth promotion were greater with mixtures than with individual PGPR strains.

scholarly journals Novel Bioformulations Developed from Pseudomonas putida BSP9 and Its Biosurfactant for Growth Promotion of Brassica juncea (L.)

Plants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1349
Author(s):  
Isha Mishra ◽  
Tahmish Fatima ◽  
Dilfuza Egamberdieva ◽  
Naveen Kumar Arora
Keyword(s):  
Plant Growth ◽  
Pseudomonas Putida ◽  
Brassica Juncea ◽  
Growth Promotion ◽  
Growth Parameters ◽  
Growth Parameter ◽  
Plant Growth Promoting Rhizobacteria ◽  
Threshold Level ◽  
Plant Growth Promoting ◽  
Growth Promoting

In this study, Pseudomonas putida BSP9 isolated from rhizosphere of Brassica juncea was investigated for its plant growth promoting and biosurfactant producing activities. The isolate showed the ability to produce indole acetic acid, siderophore, phosphate solubilization activity and was an efficient producer of biosurfactant. Purification (of the biosurfactant) by thin layer chromatography (TLC) and further characterization by Fourier transform infrared spectroscopy (FTIR) revealed that biosurfactant produced by the isolate belonged to the glycolipid category, which is largely produced by Pseudomonas sp. In addition, liquid chromatography-mass spectroscopy (LC-MS) analysis showed the presence of a mixture of six mono-rhamnolipidic and a di-rhamnolipidic congeners, confirming it as a rhamnolipid biosurfactant. Bioformulations were developed using BSP9 and its biosurfactant to check their impact on promoting plant growth in B. juncea. It was noted from the study that bioformulations amended with biosurfactant (singly or in combination with BSP9) resulted in enhancement in the growth parameters of B. juncea as compared to untreated control. Maximum increment was achieved by plants inoculated with bioformulation that had BSP9 plus biosurfactant. The study also suggested that growth promotion was significant up to a threshold level of biosurfactant and that further increasing the concentration did not further enhance the growth parameter values of the plant. The study proves that novel bioformulations can be developed by integrating plant growth promoting rhizobacteria (PGPR) and their biosurfactant, and they can be effectively used for increasing agricultural productivity while minimizing our dependence on agrochemicals.

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