scholarly journals Influence of plant growth regulators on the rapid propagation buds of Dendrobium officinale Kimura et Migo

2017 ◽  
Vol 1 (T2) ◽  
pp. 29-38
Author(s):  
Diem Thi Le ◽  
Mai Thi Bach Vo
Keyword(s):  
Functional Foods ◽  
Culture Medium ◽  
High Efficiency ◽  
Nodal Explants ◽  
Dendrobium Officinale ◽  
Ms Medium ◽  
The World ◽  
Long Time ◽  
Short Time

Dendrobium officinale Kimura et Migo has been known for a long time as a precious orchid, which is used for medicine and functional foods that are widely commercialized in the world. The nodal explants could be obtained in high efficiency, good quality and uniform seedlings on multiplication vegetative in vitro. They can be propagated in large quantities in a short time. The studied results showed that the nodal explants grew on MS medium + 10 % coconut milk + 25 g sucrose + 0.5 mg/L BA + 6 g agar/ liter create high buds. This buds were used to A B 1 cm 1,5 cm create bud clusters with the aim of improving in vitro vegetative. The best bud culture medium for the formation of clusters was MS + BA 2.0 mg/L + NAA 0.4 mg/L; MS + kinetin 1.5 mg/L + NAA 0.3 mg/L; MS + TDZ 2.0 mg/L + NAA 0.4 mg/L; MS + adenine 1.5 mg/L + NAA 0.3 mg/L. However, the addition of kinetin or TDZ to the culture medium needed a steps to extend the bud subculture after 45 days in culture. The best medium for the rooting is MS + NAA 0.5 mg/L.

scholarly journals Micropropagation of Phalaenopsis amabilis var. ‘Manila’ by Leaves Obtained from in Vitro Culturing the Nodes of Flower Stalks

2016 ◽  
Vol 8 (2) ◽  
pp. 164-169 ◽  
Author(s):  
Khosro BALILASHAKI ◽  
Masood GHASEMI GHEHSAREH
Keyword(s):  
In Vitro Culture ◽  
Growth Regulators ◽  
Culture Medium ◽  
Global Market ◽  
Ms Medium ◽  
Current Experiment ◽  
Flower Stalk ◽  
The World

Orchids are one of the most popular plants in the world and among them the species of Phalaenopsis have the most sales on the global market. Because of its difficult propagation, micropropagation has been suggested recently. In the current study, the leaves obtained from in vitro culture of flower stalk nodes were used as explants and were cultured on MS medium with different concentrations of NAA, BA and TDZ. Protocorm-like bodies (PLBs) were produced and transferred to medium without growth regulators. Finally, the adaptation of plants was evaluated in a medium of cocopeat + coal (3:1 v/v) and another medium of cocopeat + charcoal + LECA (2:1:2 v/v). Results showed that the highest percentage of active samples was 100% which could regenerate the PLBs by being treated with 4 mg/l TDZ. The lowest active samples (60%) were those treated in the medium with 4 mg/l BAP + 0.5 mg/l NAA. The highest PLBs per explant (50.65) were obtained in the medium supplemented with 15 mg/l BAP + 3 mg/l NAA. Best acclimation (90%) of plants was obtained when medium of cocopeat + charcoal + LECA (2:1:2 v/v) was used. According to the results of the current experiment, the MS culture medium containing 15 mg/l BAP + 3 mg/l NAA was thereby considered as the best medium for Phalaenopsis micropropagation.

scholarly journals Expression Level of Mature miR172 in Wild Type and StSUT4-Silenced Plants of Solanum tuberosum Is Sucrose-Dependent

2021 ◽  
Vol 22 (3) ◽  
pp. 1455
Author(s):  
Varsha Garg ◽  
Aleksandra Hackel ◽  
Christina Kühn
Keyword(s):  
Solanum Tuberosum ◽  
Wild Type ◽  
Potato Plants ◽  
Mature Leaves ◽  
In Vitro Plantlets ◽  
Long Time ◽  
Short Time ◽  
Cut Leaves

In potato plants, the phloem-mobile miR172 is involved in the sugar-dependent transmission of flower and tuber inducing signal transduction pathways and a clear link between solute transport and the induction of flowering and tuberization was demonstrated. The sucrose transporter StSUT4 seems to play an important role in the photoperiod-dependent triggering of both developmental processes, flowering and tuberization, and the phenotype of StSUT4-inhibited potato plants is reminiscent to miR172 overexpressing plants. The first aim of this study was the determination of the level of miR172 in sink and source leaves of StSUT4-silenced as well as StSUT4-overexpressing plants in comparison to Solanum tuberosum ssp. Andigena wild type plants. The second aim was to investigate the effect of sugars on the level of miRNA172 in whole cut leaves, as well as in whole in vitro plantlets that were supplemented with exogenous sugars. Experiments clearly show a sucrose-dependent induction of the level of mature miR172 in short time as well as long time experiments. A sucrose-dependent accumulation of miR172 was also measured in mature leaves of StSUT4-silenced plants where sucrose export is delayed and sucrose accumulates at the end of the light period.

scholarly journals GROWTH OF ‘PRATA-ANA’ BANANA’S MICROSHOOTS CLONE GORUTUBA FROM SYNTHETIC SEEDS:SUBSTRATES AND BAP CONCENTRATION

2017 ◽  
Vol 39 (5) ◽  
Author(s):  
JÚLIO CÉSAR GOMES PEREIRA ◽  
SELMA SILVA ROCHA ◽  
LUCIANA CARDOSO NOGUEIRA LONDE ◽  
MARCELA CAROLINE BATISTA DA MOTA ◽  
PABLO FERNANDO SANTOS ALVES ◽  
...  
Keyword(s):  
Culture Medium ◽  
Economic Importance ◽  
Leaf Length ◽  
Naphthalene Acetic Acid ◽  
Synthetic Seed ◽  
Ms Medium ◽  
World Production ◽  
Number Of Leaves ◽  
Alginate Matrix

ABSTRACT The banana crop stands out as an activity of great social and economic importance in Brazil, which occupies the fifth place in world production. Synthetic seed production is becoming promising for a micropropagation and in vitro conservation. The aim of the study was to analyze the conversion and growth of ‘Prata-anã’ banana’s microshoots clone Gorutuba from synthetic seed in MS medium and vermiculite, different substrates and concentrations of BAP (6-benzylaminopurine) associated with ANA (acetic naphthalene acid) in the constitution of its capsule were tested. The microshoots were immersed in the sodium alginate matrix (3%) and dripped in a solution of CaCl2.2H2O (100 mM) for complexation and then in KNO3 solution (100 mM) to decomplex. The experimental design was completely randomized in a 2 x 5 factorial design (substrate x BAP concentrations), containing different substrates (MS culture medium and vermiculite) and BAP concentrations (2.22, 4.44, 6.66, 8.88 and 13.32 µmol L-1) associated with NAA (naphthalene acetic acid) 0.54 µmol L-1, totaling 10 treatments, with 4 replicates, and that each replicate containing 5 seeds. The evaluations of conversion, number of leaves, leaf length, leaf height, number of roots, root length and oxidation were performed at 30 and 60 days.The use of the MS medium provided better growth results in relation to vermiculite as substrate, in which the different BAP concentrations did not differ from each other. It was found that, in MS culture medium, BAP concentrations above 8.88 µmol L-1 in the capsule composition are not indicated for microshoots growth.

scholarly journals In vitro Flowering of Shoots Regenerated from Cultured Nodal Explants

2011 ◽  
Vol 39 (1) ◽  
pp. 84 ◽  
Author(s):  
Kantamaht KANCHANAPOOM ◽  
Suttinee JINGJIT ◽  
Kamnoon KANCHANAPOOM
Keyword(s):  
Multiple Shoots ◽  
Shoot Formation ◽  
In Vitro Flowering ◽  
Nodal Explants ◽  
Callus Growth ◽  
Ms Medium ◽  
Old Field ◽  
Gypsophila Paniculata ◽  
Initial Culture

A protocol for the regeneration of Gypsophila paniculata L. using nodal explants from 2-month-old field grown plants was established. The induction of multiple shoots was best obtained on Murashige and Skoog (MS) medium supplemented with 13.3 μM BA. Callus growth was observed on MS medium containing 44.3 μM BA. Calluses were transferred to MS medium supplemented with 2, 4-D (4.5, 13.5, 22.6 μM), NAA (5.3, 16.1, 26.8 μM) or BA (4.4, 13.3, 22.1 μM) for 2 months to induce shoot formation. After 6 weeks of initial culture, multiple shoots were regenerated from calluses cultured on MS medium supplemented with 13.3 μM BA. All regenerated shoots produced roots on 16.1 μM NAA containing MS medium within 4 weeks. Rooted plantlets were hardened and established in pots at 100% survival. For induction of in vitro flowering, regenerated shoots could be induced to flower efficiently when cultured on MS medium containing 13.3 μM BA and 50 g/l sucrose.

scholarly journals STUDY ON THE IN VITRO MORPHOGENETIC RESPONSE FROM SHOOT AND CALLUS CULTURES OF Blepharis maderaspatensis (L.) Heyne ex. Roth

2020 ◽  
Vol 1 (40) ◽  
pp. 28-38
Author(s):  
Trang Phuong Nguyen Thi ◽  
Quang Minh Bui ◽  
Hai Duc Le ◽  
Linh Quoc Nguyen
Keyword(s):  
High Efficiency ◽  
Raw Materials ◽  
Callus Cultures ◽  
Culture Conditions ◽  
Ms Medium ◽  
Axillary Shoots ◽  
Scientific Database ◽  
Medicinal Value ◽  
Shoot Growth Rate

Blepharis maderaspatensis (L.) Heyne ex. Roth is a short-term plant which contains many important secondarycompounds with high medicinal value. Currently, most of the researches focus on chemical composition and pharmacological activity, but the source of raw materials is very limited. In this study, the first step is transferring the samples from nature into in vitro culture conditions to understand the effects of the factors related to shooting and callus morphogenesis was performed, the first node from shoots apical meristem was isolated and sterilized with 1.5% NaOCl for 20 minutes to achieve high efficiency with 86.11% sterile samples and 85.56% shoot growth rate after 2 weeks of culture on MS medium. The shoot generation from axillary shoots was continued to be investigated with the highest number of shoots formed on MS medium supplemented with BA (1 mg / l) showed 1.53 shoots/implant which the height and the number of leavesare 3.65cm and 6.67, respectively. Besides, the formation of callus from leaves of MS medium supplemented with 2.4 - D (0.25 mg / l) achieved the rate of 66.67% of cultured samples, forming good callus after 4 weeks of culture. The results of the study not only contribute importantly to understanding morphogenesis for micropropagation purposes but also serve as the scientific database for further studies at the cellular and molecular levels of this plant.

scholarly journals INDUKSI KALUS PASAK BUMI (Eurycoma longifolia Jack) MELALUI EKSPLAN DAUN DAN PETIOL

2016 ◽  
Vol 6 (1) ◽  
pp. 33
Author(s):  
ROSMAINA ROSMAINA ◽  
ZULFAHMI ZULFAHMI ◽  
PROBO SUTEJO ◽  
ULFIATUN ULFIATUN ◽  
MAISUPRATINA MAISUPRATINA
Keyword(s):  
Slow Growth ◽  
Callus Formation ◽  
Germination Percentage ◽  
Ms Medium ◽  
Petiole Explants ◽  
Eurycoma Longifolia ◽  
Yellow Color ◽  
Eurycoma Longifolia Jack ◽  
Short Time

One of the problem of Eurycoma longifolia Jack propagation was low germination percentage due to recalcitrant seeds and slow growth of seedling from cutting propagation. To overcome this problem is required propagation of Eurycoma longifolia via in vitro culture. The objective of this research was to know the effect of Auxin (2,4-D and NAA) and Cytokines (BAP and Kinetin)  on Eurycoma longifolia callus induction via leaf and petiole explants. In this study, we used plant growth regulator of 2,4 D, NAA, BAP and Kinetin in several levels.  The observed variables were appearing callus time, callus color and callus texture. The results of this study showed that MS medium supplemented with 1 ppm NAA+ 1 ppm BAP was able to induce callus formation in leaf explant for 6 months after culture. While MS medium supplemented with 1 ppm 2,4-D, 1 ppm BAP, combination of 2,4-D and Kinetin and combination of 2,4-D and BAP can induce callus formation from petiole. All the callus formation has yellow color and yellow brown color. The petiole explant that is grown in MS medium supplemented with 1 ppm BAP induced of callus in short time (18 days after culture).

scholarly journals Mass Propagation of Feronia limonia L. through Tissue Culture

1970 ◽  
Vol 45 (1) ◽  
pp. 75-78 ◽  
Author(s):  
Shahina Islam ◽  
Mosfequa Zahan ◽  
Shahina Akter ◽  
Tanjina Akhtar Banu ◽  
Ahashan Habib ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Key Words ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Nodal Explants ◽  
Ms Medium ◽  
Ex Vitro ◽  
Mass Propagation

An efficient mass propagation method for Feronia limonia was developed from excised shoot tips and nodal explants of in vitro grown seedlings. Explants were cultured on MS medium with different conc. of NAA, Kn, IAA and BAP singly or in combinations. Highest number of micro shoots and better plant growth were obtained from these two explants on MS medium supplemented with 0.2 mg/l BAP alone. The regenerated shoots were successfully rooted on MS medium supplemented with 0.5 mg/l NAA. The in vitro raised plantlets were successfully established in soil following the formation of roots with 100% survivability under ex vitro condition. Key words: Feronia limonia; Mass propagation; Node; Shoot tips; Multiple shoot DOI: 10.3329/bjsir.v45i1.5186 Bangladesh J. Sci. Ind. Res. 45(1), 75-78, 2010

scholarly journals Effect of Roasting and Brewing on the Antioxidant and Antiproliferative Activities of Tartary Buckwheat

Foods ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1331
Author(s):  
Ji-yeon Ryu ◽  
Yoonseong Choi ◽  
Kun-Hwa Hong ◽  
Yong Suk Chung ◽  
Somi Kim Cho
Keyword(s):  
Breast Cancer Cells ◽  
Room Temperature ◽  
Tartary Buckwheat ◽  
Flavonoid Content ◽  
Total Polyphenol ◽  
Antiproliferative Effects ◽  
Long Time ◽  
Short Time ◽  
Antiproliferative Activities

We evaluated the effect of the roasting and brewing conditions of Tartary buckwheat (TB), which is widely used in infusion teas, on its antioxidant and antiproliferative activities in vitro. TB was roasted at 210 °C for 10 min and brewed at a high temperature for a short time (HTST; 85–90 °C, 3 min) or at room temperature for a long time (RTLT; 25–30 °C, 24 h). Roasted TB (RTB) tea brewed at RTLT had the highest total polyphenol content (TPC) and total flavonoid content (TFC) among the four TB teas for different roasting and brewing conditions. Moreover, RTB brewed at RTLT showed the greatest 2,2-diphenyl-1-picrylhydrazyl-, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)-, and alkyl-scavenging activities. The TB tea brewed at RTLT had higher Fe2+-chelating activity than that brewed at HTST, irrespective of roasting. Moreover, RTB tea brewed at RTLT inhibited the proliferation of human pancreatic and breast cancer cells. Overall, RTB-RTLT displayed the largest effect on antioxidant and antiproliferative effects. Finally, rutin was found to possess the most pronounced effect on the antioxidant and antiproliferative activities of the TB teas. These results indicate that the antioxidant and antiproliferative activities of RTB are enhanced by RTLT brewing.

scholarly journals Antioxidant and Anti-Diabetic Activities of Polysaccharides from Guava Leaves

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1343 ◽  
Author(s):  
You Luo ◽  
Bin Peng ◽  
Weiqian Wei ◽  
Xiaofei Tian ◽  
Zhenqiang Wu
Keyword(s):  
Antioxidant Activity ◽  
High Fat Diet ◽  
Functional Foods ◽  
Radical Scavenging ◽  
Psidium Guajava ◽  
Diabetic Mice ◽  
Total Antioxidant ◽  
Long Time ◽  
Guava Leaves

Guava (Psidium guajava L., Myrtaceae) leaves have been used as a folk herbal tea to treat diabetes for a long time in Asia and North America. In this study, we isolated polysaccharides from guava leaves (GLP), and evaluated its antioxidant activity in vitro and anti-diabetic effects on diabetic mice induced by streptozotocin combined with high-fat diet. The results indicated that GLP exhibited good DPPH, OH, and ABTS free-radical scavenging abilities, and significantly lowered fasting blood sugar, total cholesterol, total triglycerides, glycated serum protein, creatinine, and malonaldehyde. Meanwhile, it significantly increased the total antioxidant activity and superoxide dismutase (SOD) enzyme activity in diabetic mice, as well as ameliorated the damage of liver, kidney, and pancreas. Thus, polysaccharides from guava leaves could be explored as a potential antioxidant or anti-diabetic agents for functional foods or complementary medicine.

scholarly journals In vitro propagation of Citrullus lanatus Thumb. from nodal explants culture

1970 ◽  
Vol 8 (2) ◽  
pp. 203-206 ◽  
Author(s):  
MM Khatun ◽  
MS Hossain ◽  
MA Haque ◽  
M Khalekuzzaman
Keyword(s):  
Citrullus Lanatus ◽  
In Vitro Regeneration ◽  
Shoot Proliferation ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Root Induction ◽  
Ms Medium ◽  
Nodal Explant ◽  
Grown Plant

A standard protocol was established for rapid in vitro propagation of watermelon (Citrullus lanatus Thumb.) from nodal explants of field grown plant. Multiple shoot proliferation was achieved from nodal explants on MS medium supplemented with 1.0 mg/l BAP + 0.2 mg/l NAA within 30 days of inoculation. The elongation of shoots was obtained on the same medium. Highest percentage of root induction was achieved on MS medium supplement with 1.0 mg/l IBA within 25 days of culture. Well rooted plantlets were transferred to small pots and after proper acclimatization the plantlets were transplanted in the field condition, where 80% plantlets were survived and grew successfully. Keywords: In vitro regeneration; Nodal explant; Citrullus lanatus DOI: 10.3329/jbau.v8i2.7926 J. Bangladesh Agril. Univ. 8(2): 203-206, 2010  

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