scholarly journals Combined cellulolytic and pectinolytic enzymes to increase the polyphenol extractability of coffee husks

Author(s):  
Trà Thị Thu Trần ◽  
Trần Minh Khánh ◽  
Tôn Nữ Minh Nguyệt ◽  
Lê Văn Việt Mẫn

Coffee husks (CHs) is a high biological activity by-product of the coffee bean industry, but currently, in Vietnam, it has not been used properly in the food industry. This study was conducted to investigate the combined cellulolytic and pectinolytic enzymes to increase the polyphenol extractability of coffee husks. The red ripe Robusta coffee cherries from Dăklak province, Vietnam were blanched, peeled and the coffee husks was then extracted. The effects of pectinase content (from 0 to 800 U/gCHs), cellulase content (from 0 to 800 U/gCHs), pH (from 3.0 to 5.0), temperature (from 40◦C to 60◦C), CHs: solvent ratio (from 1: 6 to 1:12) and extracted time (from 60 minutes to 120 minutes) on soluble solid content (SSC), total polyphenol content (TPC) and antioxidant activity were investigated. The appropriate conditions for the treatment were pectinase dosage of 600 U/gCHs, cellulase dosage of 400 U/gCHs, pH 4.0, temperature 50◦C, CHs:soluble ratio = 1:10 and incubation time of 60 min under which the SSC, TPC and antioxidant activity of extract were highest by 60.60.4 g/100g dry weight of CHs, 200.5 gGAE /100g dry weight of CHs, 1.5 +/- 0.1 mMTE/g dry weight of extract (according to DPPH assay) and 1.5 +/- 0.1 mMTE/g dry weight of extract (according to DPPH assay). From this result, it can be seen that the extracted solution from coffee husk is a potential source for the production of soft drinks with high antioxidant activity.

2020 ◽  
Vol 15 (10) ◽  
pp. 1934578X2096843
Author(s):  
In-Hee Lee ◽  
Ho-Sung Lee ◽  
Kyungrae Kang ◽  
Sang-In Park ◽  
Tae-wook Kwon ◽  
...  

FDY2004 (Medicinal herbs: Rheum palmatum, Paeonia suffruticosa Andrews, and Prunus davidiana), an herbal drug composition with an antiproliferative effect, is prepared by boiling, which is the most common herbal extraction method in traditional Korean medicine. Several parameters are considered in the process, including herb-to-solvent ratio, extraction temperature and pressure, and total decoction time. The aim of this study was to examine the physicochemical changes, index compound analysis results, antioxidant activity, and antiproliferative activity of FDY2004 according to the decoction duration to establish the conditions that ensure efficacy while minimizing side effects. Different samples of FDY2004 were obtained by decocting for 30, 60, 90, 120, 180, and 240 minutes. Each sample was evaluated for hydrogen ion concentration (pH), total soluble solid content (TSSC), index compound profiles, and antioxidative and antiproliferative activity. pH was found to decrease, while TSSC increased with an increase in decoction duration. Index compound contents for FDY2004 (aloe emodin, emodin, rhein, chrysophanol, physcion, and sennoside A for R. palmatum, paeonol for P. suffruticosa Andrews, and amygdalin for P. davidiana) increased when the decoction duration was 120 minutes or more, while the content of sennoside A did not increase. The total d-glucose amount increased with an increase in boiling duration. Antioxidant activity of FDY2004 increased when the decoction duration was 120 minutes or more, and the antiproliferative activity of FDY2004 was concentration dependent. The decoction duration for FDY2004 needs to be carefully determined so as to maintain efficacy while reducing side effects related to digestive absorption.


Author(s):  
Tram Thi Hoai Pham ◽  
Tra Thi Thu Tran

Coffee husks is a typical by-product in the coffee bean industry. This by-product has a high biological activity, but recently in Vietnam, it has been used as fuel, fertilizer, and substrate for mushroom cultivation. This study aims to identify conditions for extracting polyphenol from ripe robusta coffee husks from Dăklak province. Factors affecting the extraction process are pH, temperature, coffee husks : solvent ratio, and time. The obtaining data showed that extracting at pH=4, 50oC, with the solvent:coffee husks ratio = 7:1, during 1 hour would contribute the best results in terms of an extraction efficiency of soluble solids (33 %), the extraction efficiency of polyphenol (94 %), antioxidant activity according to DPPH assay (1959 ± 168 µM TE/ g dry matter of extracted solution) and FRAP assay (1976 ± 117 µM TE/ g dry matter of extracted solution). In addition, the extracted solution from the coffee husk is a potential source of polyphenol, as well as the antioxidant capacity that could be applied to future researches..  


HortScience ◽  
2012 ◽  
Vol 47 (5) ◽  
pp. 599-602 ◽  
Author(s):  
Satoru Motoki ◽  
Hiroaki Kitazawa ◽  
Tomonori Kawabata ◽  
Hiroaki Sakai ◽  
Ken-ichi Matsushima ◽  
...  

Head tightness, soluble solid content (SSC), and rutin level were investigated in asparagus spears harvested at different lengths. We found no correlation between spear length and SSC per dry weight (DW) in spearheads. Spearheads became looser and the rutin content of the spearhead increased as spear length at harvest increased, although appearance quality decreased. These findings revealed that spears previously discarded, because their length did not meet the length specifications, contained abundant rutin. Therefore, spears for which the optimum harvesting time has been missed are a useful rutin source.


HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1048A-1048
Author(s):  
Kuo-Tan Li ◽  
Jim Syvertsen ◽  
Jill Dunlop

Effects of crop load on leaf characteristics, shoot growth, fruit shape, fruit quality, and return bloom were investigated in 13-year-old `Ruby Red' grapefruit (Citrus paradisi Macf.) on `Swingle' citrumleo rootstock. Trees were hand thinned in June 2003 and 2004 at the end of physiological fruit drop to establish three to four levels of crop load ranging from normal (high crop load without thinning) to extremely low (near 90% fruit removal). Leaves on high crop load trees had higher net assimilation of CO2 (ACO2) than those on low crop load trees. Crop load enhancement of ACO2 continued until harvest. In 2004, however, the effects were diminished in October just prior to the beginning of the harvest season, after leaf and fruit loss from three consecutive hurricanes. There was no difference in leaf dry weight per leaf area and leaf nitrogen among treatments. Nonfruiting branches of high crop load trees produced fewer, but longer, summer flushes than those of low crop load trees. Fruiting branches generally produced few summer flushes with similar shoot lengths among treatments. High crop load trees developed a greater percentage of vegetative shoots, whereas low crop load trees developed more inflorescences. Crop load adjustments did not affect fruit size and total soluble solid content, but low crop load trees produced a higher percentage of irregular shape (sheepnosed) fruit with high acidity.


Agronomy ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 884 ◽  
Author(s):  
Vittorio Farina ◽  
Carla Gentile ◽  
Giuseppe Sortino ◽  
Giuseppe Gianguzzi ◽  
Eristanna Palazzolo ◽  
...  

Some of the key components that contribute to the acceptance of high-quality fresh mangoes by consumers are its flavour, odour, texture and chemical constituents that depend mainly on level of maturity. In the European market, the demand for tree-ripened fruit has increased in recent decades. Nevertheless, the qualitative response and the marketable characteristics of tree-ripened mango fruit grown in the Mediterranean area are not yet studied. Tree-ripened fruits of cv Keitt, Glenn, Osteen, Maya, Kensington Pride and Tommy Atkins were submitted to analytical (fruit weight, transversal diameter, longitudinal diameter, flesh firmness, total soluble solid content, titratable acidity, seed weight, peel weight, percentage of flesh and fibre, ash content, fat content, carbohydrate content, riboflavin, niacin, thiamin, K, Na, Ca, Mg, Fe, Cu, Mn and Zn contents, ascorbic acid and vitamin A) and sensory evaluations. Significant differences were observed for sensory and physicochemical characteristics in a cultivar-dependent manner. The mango Keitt, in addition to its interesting physicochemical traits, content of protein and vitamin, has a higher sensory appeal. Glenn fruit showed more suitable weight, flesh firmness, soluble solids, vitamin content, total antioxidant activity and total polyphenolics content; Maya had the lowest titratable acidity and the highest soluble solid content, whereas Tommy Atkins revealed preferable ground colour, total antioxidant activity, and vitamin B2 and vitamin C contents. Tree-ripened fruits grown in the Mediterranean climate show interesting peculiarities in regard to fresh market requirements. The analytical-sensory approach established a qualitative characterization of the six observed cultivars.


2011 ◽  
Vol 16 (3) ◽  
pp. 282 ◽  
Author(s):  
Daniel Borda-Molina ◽  
Juan Manuel Pardo-García ◽  
José Salvador Montaña-Lara ◽  
María Mercedes Martínez-Salgado

<p><strong> </strong></p> <p><strong> </strong><em>Stevia rebaudiana</em> is a plant that accumulates a non-caloric sweetener compound known as stevioside. This crop is traditionally fertilized with chemicals that are harmful for the ecosystem, forcing to find  organic alternatives to mitigate this damage. <strong>Objective</strong>. To study the effect of organic matter and an <em>Azotobacter nigricans</em>-based bio-fertilizer on a <em>Stevia rebaudiana </em>plantation grown in acidic soil in the Department of Meta, Colombia. <strong>Materials and methods.</strong> Five treatments were established: T1 and T2 with the application of home organic waste compost at concentrations of 15 and 30 ton ha<sup>-1</sup>. T3 and T4 with the same compost concentrations and inoculating the<em> A. nigricans </em>bio-fertilizer. T5 contained the bio-fertilizer alone. The control consisted of the application of the Bokashi compost under the usual conditions of cultivation.  Plant growth was assessed by biomass increase measured as dry weight, production, and leaf area.  The physicochemical analysis of soil included: percentage of organic carbon, water content, and pH. <strong>Results. </strong>The inoculation of the bio-fertilizer produced an increase in the rate of mineralization of compost, reaching a final 4.85% of OC between 90 and 180 days after inoculation. <strong> </strong>There were significant (p&lt; 0.05) differences between biomass production with T2 (1,538 kg ha<sup>-1</sup>) and the control (477 kg ha<sup>-1</sup>).  Regarding the soluble solid content, T1, T3 and T4 showed the highest °Brix values (12.4, 12.35 and 12.15, respectively).  <strong>Conclusions</strong>. The concentration of 30 ton ha<sup>-1</sup> produced the highest biomass production and the application of the biofertilizer showed a positive correlation with compost mineralization and glucoside synthesis.</p> <p><strong> </strong></p> <p><strong>Key words</strong>: <em>Stevia rebaudiana</em>, bio-fertilizer, mineralization, organic carbon.</p> <p> </p><br />


2020 ◽  
Vol 19 (2) ◽  
pp. 102
Author(s):  
Fitri Yelli ◽  
Hanisah Hanisah ◽  
Rusdi Evizal ◽  
Sugiatno Sugiatno

Coffee husk is the main waste of coffee bean production using a dry processing system. Coffee husk is returned to the coffee plantation as mulch, or used as a mixture for nursery media and for making bio-charcoal. This study aims to determine the effect of the formulation of biochar, coffee husk, and soil on the growth of Arabica coffee seedlings. The experiment used a randomized block design (RBD) consisting of 6 treatments with 4 replications. The treatments were the composition of the ratio of biochar: coffee husk: soil (v / v / v), namely: 0: 1: 2 (control, without biochar), 1: 1: 2, 1: 0: 2 (without coffee skin), 2: 1: 2, 1: 2: 2, and 1: 1: 1. The coffee husk biochar is made by a slow burning system in a stack. The results showed that: (1) The treatment of biochar composition, coffee husk and soil had an effect on the number of leaves, length x leaf width, crown dry weight, fresh weight quality index, and dry weight quality index; (2) biochar mixing treatment did not increase seedling growth. Mixing biochar with a composition of 1: 1: 2 reduces root growth; (3) the composition of biochar, coffee husk and soil 1: 1: 2 gave better seedling growth than the composition 0: 1: 2 (control without biochar) based on the quality index of fresh seedling and dry seedling.Keywords: Arabica, biochar, coffee husk, growth, seedling, quality indeces


2020 ◽  
Vol 36 (3) ◽  
Author(s):  
Caroline Farias Barreto ◽  
Renan Navroski ◽  
Letícia Vanni Ferreira ◽  
Jorge Atílio Benati ◽  
Marcelo Barbosa Malgarim ◽  
...  

Nitrogen, which is considered the most important nutrient for peach trees, may interfere in both quantitative production characteristics and quality of fruits. This study aimed at evaluating the effect of the combination of doses of N fertilization and different periods of cold storage on physico-chemical and phytochemical characteristics of peaches in post-harvest. The experiment had a randomized complete block design in a 4x3 factorial scheme, i. e., four doses of fertilization (0, 60, 120 and 180 Kg N ha-1) and three periods of storage (on the harvest day, on both the 15th and the 30th storage days at 1±1ºC, each followed by a day of simulated commercialization at 20±1ºC). The following aspects were evaluated in fruits yielded by peach trees of the genotype Cascata 1067: fruit color, soluble solid content, titratable acidity, pulp firmness, mass loss, total phenolic compounds and antioxidant activity. Different periods of cold storage and doses of N fertilization were found to affect epidermis luminosity, pulp firmness and titratable acidity of fruits. Peaches may be stored at low temperatures for 15+1 days. After that, loss of fruit firmness increases. N fertilization affects neither the soluble solid content nor the epidermis color of peaches, but both parameters are influenced by storage. Values of phenolic compounds and antioxidant activity decrease when there is N increment in the soil and when longer storage is carried out. Results suggest that peach composition may be affected by cultural practices, such as N fertilization, in harvest and after storage.       


2016 ◽  
Vol 848 ◽  
pp. 85-88
Author(s):  
Natthiya Chaichana

Pittosporoposis kerrii Craib is the consumed plant of Maechaedee Subdistrict, Wiang Pa Pao District, Chiang Rai Province. The area of survey is households that plant P. kerrii Craib for consuming and selling with the highest number of 200 trees per household. Therefore, this plant is increasingly interested in evaluation of the useful consumption. The purpose of this study was to investigate antioxidant and nutrition facts of P. kerrii Craib. For nutrition facts per 100 g dry weight, the result revealed that P. kerrii Craib seed extraction contained 2.70 g of protein, 37.79 g of carbohydrate, 1.83 g of lipid, 1.36 g of crude fiber, 1.20 g of ash and 191.4 kcal of energy. The contents of vitamins were examined. It was found that P. kerrii Craib seeds contained vitamins B1 and B2 (0.018 and 0.092 mg per 100 g dry weight, respectively). Antioxidant was determined by DPPH assay method. The results showed that P. kerrii Craib seed extract had the extremely high antioxidant activity (91.20% inhibition). The experiment exposed that P. kerrii Craib seeds provided the useful consumption and should be promoted to support local economic for sustainable development.


Agriculture ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 82 ◽  
Author(s):  
Rosario Paolo Mauro ◽  
Valeria Rizzo ◽  
Cherubino Leonardi ◽  
Agata Mazzaglia ◽  
Giuseppe Muratore ◽  
...  

The present experiment addressed the effects of two harvest stages, namely breaker (S1) and turning (S2), on compositional and sensory profile of grafted, greenhouse elongated tomatoes cv. “Sir Elyan”. The rootstocks “He-Man”, “Interpro”, and “Armstrong” were used. The S1 fruits showed a higher dry matter content, firmness and titratable acidity when compared to the S2 ones (by 6%, 3%, and 15%, respectively). They showed, also, the highest L-ascorbic acid concentration and antioxidant activity. Differently, soluble solid content, lycopene and β-carotene increased in the S2 fruits (by 4%, 92%, and 26%, respectively). Excepting methyl salicylate, all the volatiles detected peaked in the S2 fruits, which were scored by panelists as sweeter and more flavorful than the S1 ones. Among the rootstock genotypes, “He-Man” promoted fruits firmness, carotenoids concentration and antioxidant activity, irrespective of the harvest stage. “Interpro” enhanced the sensory attributes of the S1 fruits in terms of bitterness, sourness, sweetness and flavor. Differently, sweetness, sourness, and tomato flavor of the S2 fruits were promoted by “Armstrong”. The results highlight the influence of rootstock genotype on the nutraceutical and sensorial profile of “Sir Elyan” fruits harvested at early ripening stages.


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