scholarly journals Using invasive and non-invasive methods to evaluate the therapeutic effect of dielectric barrier discharge (DBD) Plasma in skin graft surgery

2021 ◽  
Vol 3 (SI3) ◽  
pp. first
Author(s):  
Ngo Thi Minh Hien ◽  
Do Tuyet Nhi ◽  
Nguyen Quoc Duy Nam ◽  
Vo Duong Van Anh ◽  
Nguyen Duy Phuong ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Skin Graft ◽  
Evaluation Methods ◽  
Healing Time ◽  
The Body ◽  
Therapeutic Effects ◽  
Dbd Plasma ◽  
Dielectric Barrier ◽  
Non Invasive

Skin graft is effective method to reconstruct large lesions on the skin. Long-term injuries such as burns, skin ulcers, skin flaps, skin grafts... can affect the health of the patient. Skin plays a very important role because it protects the body from dehydration, helps regulate temperature, helps prevent pathogenic bacteria or viruses from entering the body and minimizes skin deformation. In recent years the cold plasma has been used to aid the treatment of chronic skin wounds, burns, ulcers,... To assess the effectiveness of the wound healing process of Plasma, this study uses non – invasive (assess the shrinkage of the wound, the temperature change of the wound) and invasive methods (regeneration of skin tissue structure) to evaluate the therapeutic effects of Dielectric Barrier Dissections Plasma (DBD Plasma) in skin graft surgery. Experimenting with the entire skin graft model on mice, this use of DBD Plasma treatment along with clinical evaluation methods, this is to compare wound healing time between wounds treated with plasma DBD and untreated wound. After 21 days of experimentation, evaluation methods and results processing tools, it was shown that wound healing of skin lesions on mice of the wound area treated with DBD Plasma faster than nontreatment about: (1) The surface shrinkage rate is about 25%; (2) The average wound temperature is less than 0.5-1oC; (3) Healing time is faster from 5 to 6 days. Therefore, DBD Plasma is able to be a potential treatment for wound healing combined with skin graft surgery in the future. The initial study of DBD model is the premise from which could be broader in other animals such as rabbits and pigs with skin structure closer to the human skin. And further, the DBD Plasma shows the potential for preclinical application to human skin graft.

scholarly journals Healing the third degree thermal burn in mice by Dielectric Barrier Discharge Plasma (DBD plasma): Comparison and evaluation of area, burn wound healing time, burn skin temperature

10.29007/fmkc ◽  
2020 ◽  
Author(s):  
Thi Minh Hien Ngo ◽  
Tuyet Nhi Do ◽  
Quoc Duy Nam Nguyen ◽  
Duy Phuong Nguyen ◽  
Nguyen Ngan Ha Lam ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Time ◽  
Diagnostic Methods ◽  
Invasive Treatment ◽  
Burn Wound ◽  
Dbd Plasma ◽  
Thermal Burn ◽  
Non Invasive ◽  
Burn Wound Healing ◽  
Wound Healing Time

Burns are one of the most devastating conditions encountered in medicine. This injury is in skin or other tissues, caused by heat-cold, electricity or chemicals [1,2]. There are lots of methods to treat burns and each method has its own advantages, such as medicine, dressing, low-level laser, plasma, skin graft surgery... [3].This study experimented with the 3rd degree burn model in mice by heat, treating by DBD plasma, is a non-invasive treatment and using clinical diagnostic methods by (1) normal image, (2) thermal image, (3) HE staining. Aim of this research is evaluation and comparison the area, temperature and wound healing time of non-invasive treatment with DBD plasma and nontreatment. After 3-week experiment, using diagnostic methods and analysis tools have demonstrated that the 3rd thermal burn wound healing of plasma treatment recovers faster than non-treatment about: (1) Burn wound surface shrinkage rate is higher: ~ 5%; (2) Healing time is faster: 2-3 days; (3) The average temperature of the burn wound is lower: 1-2oC. Therefore, DBD plasma is a potential treatment in burns wound and wound healing in the future. Keywords: DBD plasma, burn wound, healing wound

scholarly journals Root extractive from Daphne genkwa benefits in wound healing of anal fistula through up-regulation of collagen genes in human skin fibroblasts

2017 ◽  
Vol 37 (2) ◽  
Author(s):  
Dong Yang ◽  
Jun-hua Xu ◽  
Ren-jie Shi
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Anal Fistula ◽  
Healing Time ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Protein Levels ◽  
Daphne Genkwa

Wound healing is the main problem in the therapy of anal fistula (AF). Daphne genkwa root has been traditionally used as an agent to soak sutures in operation of AF patients, but its function in wound healing remains largely unclear. The aim of the present study was to illuminate mechanisms of D. genkwa root treatment on AF. In the present study, 60 AF patients after surgery were randomly divided into two groups, external applied with or without the D. genkwa extractive. Wound healing times were compared and granulation tissues were collected. In vitro, we constructed damaged human skin fibroblasts (HSFs) with the treatment of TNF-α (10 μg/ml). Cell Count Kit-8 (CCK-8) and flow cytometry analysis were used to determine the effects of D. genkwa root extractive on cell viability, cell cycle and apoptosis of damaged HSFs. Furthermore, protein levels of TGF-β, COL1A1, COL3A1, Timp-1, matrix metalloproteinase (MMP)-3 (MMP-3) and MEK/ERK signalling pathways were investigated both in vivo and in vitro. Results showed that D. genkwa root extractive greatly shortens the wound healing time in AF patients. In granulation tissues and HSFs, treatment with the extractive significantly elevated the expressions of COL1A1, COL3A1, Timp-1, c-fos and Cyclin D1, while reduced the expression of MMP-3. Further detection presented that MEK/ERK signalling was activated after the stimulation of extractive in HSFs. Our study demonstrated that extractive from D. genkwa root could effectively improve wound healing in patients with AF via the up-regulation of fibroblast proliferation and expressions of COL1A1 and COL3A1.

scholarly journals Activation of the Normal Human Skin Cells by a Portable Dielectric Barrier Discharge-Based Reaction-Discharge System of a Defined Gas Temperature

2019 ◽  
Vol 40 (1) ◽  
pp. 79-97 ◽  
Author(s):  
Anna Dzimitrowicz ◽  
Aleksandra Bielawska-Pohl ◽  
Piotr Jamroz ◽  
Jerzy Dora ◽  
Agnieszka Krawczenko ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Chronic Wounds ◽  
Barrier Discharge ◽  
Operating Conditions ◽  
Operating Parameters ◽  
Gas Temperature ◽  
Optimal Operating ◽  
Discharge System ◽  
Dielectric Barrier

Abstract Skin injury leading to chronic wounds is of high interest due to the increasing number of patients suffering from this symptom. Proliferation, migration, and angiogenesis are key factors in the wound healing processes. For that reason, controlled promotion of these processes is required. In this work, we present the portable helium-dielectric barrier discharge (He-DBD)-based reaction-discharge system of controlled gas temperature for biological activities. To make this He-DBD-based reaction-discharge system safe for biological purposes, a multivariate optimization of the operating parameters was performed. To evaluate the effect of the He-DBD operating parameters on the rotational gas temperature Trot(OH), a design of experiment followed by a Response Surface Methodology was applied. Based on the suggested statistical model, the optimal operating conditions under which the Trot(OH) is less than 37 °C (310 K) were estimated. Then, the resulted model was validated in order to confirm its accuracy. After estimation the optical operating conditions of He-DBD operation, the spectroscopic characteristic of the He-DBD-based reaction-discharge system in relevance to the several optical temperatures in addition to electron number density has been carried out. Additionally, the qualitative and quantitative analyses of the reactive oxygen species and reactive nitrogen species were performed in order to investigate of reactions and processes running in the He-DBD-gaseous phase and in the He-DBD-treated liquid. Next, the developed portable He-DBD-based reaction-discharge system, working under the optimal operating conditions, was used to stimulate the wound healing process. It was found that a 30 s He-DBD treatment significantly increased the proliferation, migration, and angiogenesis of keratinocytes (HaCaT) and fibroblasts (MSU-1.1) cell lines, as well as human skin microvascular endothelial cells (HSkMEC.2). Hence, the application of the cold atmospheric pressure plasma generated in this He-DBD-based reaction-discharge system might be an alternative therapy for patient suffering from chronic wounds.

New Treatment of Wound Healing With Allogenic Acellular Human Skin Graft: Preclinical Assessment and In Vitro Study

2020 ◽  
Vol 52 (7) ◽  
pp. 2204-2207
Author(s):  
M. Nita ◽  
J. Pliszczyński ◽  
C. Kowalewski ◽  
K. Woźniak ◽  
A. Eljaszewicz ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Skin Graft ◽  
In Vitro Study ◽  
Vitro Study ◽  
New Treatment

Biological versus non-biological dressings in the management of split-thickness skin-graft donor sites: a systematic review and meta-analysis

2020 ◽  
Vol 29 (10) ◽  
pp. 604-610
Author(s):  
Shafiq Rahman ◽  
Benjamin Langridge ◽  
Ahmed Al-Hadad ◽  
Rehman Ali Khan ◽  
Muhammad Hyder Junejo ◽  
...  
Keyword(s):  
Systematic Review ◽  
Wound Healing ◽  
Skin Graft ◽  
Meta Analysis ◽  
Healing Time ◽  
Conclusive Evidence ◽  
Split Thickness Skin Graft ◽  
Controlled Trials ◽  
Cochrane Central Register ◽  
Thickness Skin

Objective: There are currently no definitive guidelines regarding the management of split-thickness skin-graft (STSG) donor sites. The literature reports biological and non-biological dressings as the two main groups; however, there is no conclusive evidence regarding the ideal type. A systematic review and meta-analysis of existing clinical trials was performed to compare biological and non-biological dressings in managing STSG donor sites. Method: The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement standards was used to conduct this study. Electronic databases including MEDLINE, Embase, CINAHL and the Cochrane Central Register of Controlled Trials (CENTRAL) were searched by two authors (SR and BL). Data analysis was performed with RevMan 5.3. Results: In total, 10 studies, consisting of eight randomised controlled trials and two observational assessments, were identified. Wound healing time was faster with biological dressings compared to non-biological dressings (mean difference -5.44 days; p<0.05). A higher epithelialisation rate was also noted for biological dressings. There was no difference in the infection rate between the two study groups (odds ratio [OR] 0.39; 95% confidence interval [CI] 0.15–1.04) or wound exudation (OR 0.31; 95% CI 0.01–8.28). The pain level experienced during dressing changes in both groups was reported to be similar. Conclusion: The rate of epithelialisation and wound healing is greater for STSG donor sites when treated with biological dressings, but they offer no difference in terms of reducing pain, limiting infection or exudation.

scholarly journals POTENTIAL OF NANOCARRIERS FOR WOUND HEALING

2021 ◽  
Vol 13 ◽  
pp. 23
Author(s):  
Kamla pathak
Keyword(s):  
Wound Healing ◽  
Healing Time ◽  
Anatomical Structure ◽  
The Body ◽  
Anatomical Location ◽  
First Line ◽  
Outermost Layer ◽  
Functional Integrity

A wound refers to the loss in normal anatomical structure and functional integrity of the skin which is an outermost layer and first line defense of the body. It can be described by different ways based on its etiology, anatomical location, healing time, and level of contamination.

Phytolaserophoresis as Integrative Approach to the Treatment of Chronic Internal Organs Diseases

10.12737/5764 ◽  
2014 ◽  
Vol 8 (1) ◽  
pp. 1-7
Author(s):  
Касаева ◽  
E. Kasaeva ◽  
Купеев ◽  
R. Kupeev ◽  
Купеев ◽  
...  
Keyword(s):  
Self Regulation ◽  
The Body ◽  
Integrative Approach ◽  
Therapeutic Effects ◽  
Primary Role ◽  
Endocrine Regulation ◽  
Material Resources ◽  
Non Invasive ◽  
Regulatory Functions

This article describes the development history and basic principles of a modern therapy method - phytolaserophoresis. It provides complex treating impact on chronic diseases, taking into account additional pathogenic disorders of nerve and endocrine regulation. Data analysis of patients with various internal chronic pathologies, revealed the primary role of osteochondrosis and other degenerative disc diseases in maintaining chronic pathological processes. Osteochondrosis doesn’t an isolated problem of the musculoskeletal system, it leads to hypoxia of the brain, the excitement of sub-cortical centers of self-regulation and, as a consequence, in violation of the nervous and endocrine regulation. Treatment by means of phytolaserophoresis method implies soft methods of therapeutic effects on central and peripheral factors to chronic disease. After normalization of the regulatory functions of the body, against the background of recovery of homeostasis and microcirculation in organs, the targeted therapeutic effect at the site of the problem becomes more efficient and can achieve a significant improvement and long-term remission. Advantages of the technique are its non-invasive, absence side effects, available technical and material resources and the possibility of applying the method in medical establishments of any capacity.

Treating skin graft donor sites: a comparative study between remnant skin use and polyurethane foam

2019 ◽  
Vol 28 (7) ◽  
pp. 469-477
Author(s):  
Sae Hwi Ki ◽  
Sung Hwan Ma ◽  
Jong Hwan Choi ◽  
Seung Hyun Sim ◽  
Hyung Muk Kim
Keyword(s):  
Wound Healing ◽  
Patient Satisfaction ◽  
Polyurethane Foam ◽  
Skin Graft ◽  
Donor Site ◽  
Healing Time ◽  
Pain Patient ◽  
Donor Site Pain ◽  
Graft Donor ◽  
The Aesthetic

Objective: Excess remnant skin is retained for use in additional grafting in case of split-thickness skin graft (STSG) failure. We hypothesise that regrafting with remnant skin offers greater efficacy and advantages in wound healing and donor site appearance. Methods: Skin graft donor sites were assessed by comparing those regrafted with remnant skin with those treated with polyurethane foam dressing. Healing time, pain, patient satisfaction, itching sensation, skin stiffness and irregularity between regrafting and foam dressing were compared. The aesthetic satisfaction of donor site was evaluated by four board-certified plastic surgeons. The differences were tested statistically. Results: A total of 39 patients received a STSG due to skin or soft tissue wounds caused by burn, trauma and cancer reconstruction. The donor site healing time was shorter with remnant skin regrafting compared with foam dressing. There was no difference with respect to donor site pain between the two treatment groups. At two weeks after skin graft, patient satisfaction was higher in those treated with remnant skin than in those treated with foam dressing. Aesthetic assessment was improved after 12 weeks. Conclusion: Donor site dressing using remnant skin appears to improve wound healing and enhance the aesthetic outcome of donor sites.

scholarly journals Synthetic Retinoid Seletinoid G Improves Skin Barrier Function through Wound Healing and Collagen Realignment in Human Skin Equivalents

2020 ◽  
Vol 21 (9) ◽  
pp. 3198 ◽  
Author(s):  
Eun-Soo Lee ◽  
Yujin Ahn ◽  
Il-Hong Bae ◽  
Daejin Min ◽  
Nok Hyun Park ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Barrier Function ◽  
Second Harmonic ◽  
Skin Barrier ◽  
The Body ◽  
Skin Barrier Function ◽  
Extrinsic Factors ◽  
Impaired Wound Healing ◽  
Skin Equivalents

The outer epidermal skin is a primary barrier that protects the body from extrinsic factors, such as ultraviolet (UV) radiation, chemicals and pollutants. The complete epithelialization of a wound by keratinocytes is essential for restoring the barrier function of the skin. However, age-related alterations predispose the elderly to impaired wound healing. Therefore, wound-healing efficacy could be also considered as a potent function of an anti-aging reagent. Here, we examine the epidermal wound-healing efficacy of the fourth-generation retinoid, seletinoid G, using HaCaT keratinocytes and skin tissues. We found that seletinoid G promoted the proliferation and migration of keratinocytes in scratch assays and time-lapse imaging. It also increased the gene expression levels of several keratinocyte proliferation-regulating factors. In human skin equivalents, seletinoid G accelerated epidermal wound closure, as assessed using optical coherence tomography (OCT) imaging. Moreover, second harmonic generation (SHG) imaging revealed that seletinoid G recovered the reduced dermal collagen deposition seen in ultraviolet B (UVB)-irradiated human skin equivalents. Taken together, these results indicate that seletinoid G protects the skin barrier by accelerating wound healing in the epidermis and by repairing collagen deficiency in the dermis. Thus, seletinoid G could be a potent anti-aging agent for protecting the skin barrier.

scholarly journals Antioxidant Effects of Anthocyanin-Rich Riceberry™ Rice Flour Prepared Using Dielectric Barrier Discharge Plasma Technology on Iron-Induced Oxidative Stress in Mice

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4978
Author(s):  
Natwalinkhol Settapramote ◽  
Niramon Utama-ang ◽  
Touchwin Petiwathayakorn ◽  
Kornvipa Settakorn ◽  
Saovaros Svasti ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dielectric Barrier Discharge ◽  
Barrier Discharge ◽  
Thiobarbituric Acid ◽  
Rice Flour ◽  
The Body ◽  
Plasma Technology ◽  
Dbd Plasma ◽  
Dielectric Barrier ◽  
Barrier Discharge Plasma

Redox-active iron generates reactive oxygen species that can cause oxidative organ dysfunction. Thus, the anti-oxidative systems in the body and certain dietary antioxidants, such as anthocyanins, are needed to control oxidative stress. We aimed to investigate the effects of dielectric barrier discharge (DBD) plasma technology in the preparation of Riceberry™ rice flour (PRBF) on iron-induced oxidative stress in mice. PRBF using plasma technology was rich in anthocyanins, mainly cyanidine-3-glucoside and peonidine-3-glucoside. PRBF (5 mg AE/mg) lowered WBC numbers in iron dextran (FeDex)-loaded mice and served as evidence of the reversal of erythrocyte superoxide dismutase activity, plasma total antioxidant capacity, and plasma and liver thiobarbituric acid-reactive substances in the loading mice. Consequently, the PRBF treatment was observed to be more effective than NAC treatment. PRBF would be a powerful supplementary and therapeutic antioxidant product that is understood to be more potent than NAC in ameliorating the effects of iron-induced oxidative stress.

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