scholarly journals Study on the antioxidant cosmetic preparation from Spirulinaplatensis and gold nanoparticles

2015 ◽  
Vol 18 (2) ◽  
pp. 36-45
Author(s):  
Lan Thi My Nguyen ◽  
Quyen Thi Hoang Nguyen ◽  
Tham Thi Mong Doan ◽  
Loan Thi Thanh Le ◽  
Hieu Trung Tran ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gold Nanoparticles ◽  
Vitamin C ◽  
Premature Aging ◽  
Reaction Conditions ◽  
Skin Rejuvenation ◽  
Skin Cells ◽  
Diammonium Salt ◽  
Potential Applications ◽  
Main Components

Oxidation can generate free radicals which damage important molecules including lipids, proteins, and nucleic acid, and causes premature aging. The oxidation of skin cells manifests as wrinkles, freckles and many other serious symptoms. To develop natural products with high antioxidant ability for skin care, we developed mixtures of Spirulina platensis biomass, gold nanoparticles (AuNP: 10 nm) and vitamin C at different ratios. The antioxidant activity of the mixtures was measured by ABTS (2,2′-azino-bis (3- ethylbenzothiazoline-6-sulfonic acid) diammonium salt) assay. The mixture of S. platensis (1000 ppm), AuNP (10 ppm), and vitamin C (10 ppm) showed excellent antioxidant activity with 95 % efficiency after 30 minutes and 100 % efficiency after 120 minutes under the reaction conditions. The combination of the mixture and the polyvinylpyrrolidone (PVP) binder was sterilized by gamma Co-60 radiation. The sterilization dose of 16 kGy was selected due to theabsence of aerobic microorganisms in the antioxidant cosmetic preparation. Thus, the cosmetic preparation in paste form containing three main components of S. platensis, gold nanoparticles, and vitamin C combinated with PVP binder has a potential applications in the field of cosmetics, especially for facial skin rejuvenation.

scholarly journals ANTIOXIDANT ACTIVITY OF N-HEXANE EXTRACT OF NUTMEG PLANTS FROM SOUTH ACEH PROVINCE

2017 ◽  
Vol 17 (1) ◽  
pp. 39 ◽  
Author(s):  
Binawati Ginting ◽  
Mustanir Mustanir ◽  
Hira Helwati ◽  
Lydia Septa Desiyana ◽  
Eralisa Eralisa ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Active Role ◽  
Positive Control ◽  
Hexane Extract ◽  
Chemical Components ◽  
Aceh Province ◽  
Myristica Fragrans Houtt ◽  
Main Components ◽  
Seed Crops

Abstract. It has tested the antioxidant activity of n-hexane extract respective roots, bark, fruit and seed crops of nutmeg against DPPH. Each extracted by maceration method with n-hexane. The extraction of each plant nutmeg with n-hexane extract obtained yield of n-hexane respectively 8,35%; 81,5%; 11,89% and 55,63%. The result of the antioxidant activity of n-hexane extract of each plant nutmeg against DPPH with a concentration of 25 ppm, 50 ppm and 100 ppm is obtained IC50, respectively, are 0,216 ppm, 63,755 ppm, 43,998 ppm and 11,599 ppm and positive control vitamin C ( IC50 = 3,657 ppm). Vitamin C is a standard compound is more often used than butyl for very high antioxidant activity. The antioxidant activity increases from high to low at the roots, seeds, fruits and bark of plants nutmeg in a row. Based on data GC-MS chemical components of each successive extracts of roots, bark, fruit and seeds are 41, 41, 44, 43 components of secondary metabolites. The main components of each part of the plant based on data from GC-MS are a 5-Octadecanoic acid, linalool, eugenol, myristicin, methoxyeugenol. The compounds are thought to play an active role in inhibiting free radicals from DPPH. n-Hexane extract nutmeg plant potential as an antioxidant. Keywords:Nutmeg (Myristica fragrans Houtt), n-hexane extract, Antioxidant, DPPH

Synthesis of Phosphonates via Michaelis-Arbuzov Reaction

2017 ◽  
Vol 14 (6) ◽  
pp. 883-903 ◽  
Author(s):  
Boppudi Hari Babu ◽  
Gandavaram Syam Prasad ◽  
Chamarthi Naga Raju ◽  
Mandava Venkata Basaveswara Rao
Keyword(s):  
Reaction Times ◽  
Biologically Active ◽  
Fine Tuning ◽  
Arbuzov Reaction ◽  
Reaction Conditions ◽  
Solvent Type ◽  
Potential Applications ◽  
Synthetic Methodologies ◽  
Optimal Reaction ◽  
The Arbuzov Reaction

Background: Michaelis–Arbuzov reaction has played a key role for the synthesis of dialkyl or diaryl phosphonates by reacting various alkyl or aryl halides with trialkyl or triaryl phosphite. This reaction is very versatile in the formation of P-C bond from the reaction of aliphatic halides with phosphinites or phosphites to yield phosphonates, phosphinates, phosphine oxides. The Arbuzov reaction developed some methodologies, possible mechanistic pathways, selectivity, potential applications and biologically active various phosphonates. Objective: The synthesis of phosphonates via Michaelis–Arbuzov reaction with many new and fascinating methodologies were developed and disclosed in the literature, and these are explored in this review. Conclusion: This review has discussed past developments and vast potential applications of Arbuzov reaction in the synthesis of organophosphonates. As presented in this review, various synthetic methodologies were developed to prepare a large variety of phosphonates. Improvements in the reaction conditions of Lewis-acid mediated Arbuzov rearrangement as well as the development of MW-assisted Arbuzov rearrangement were discussed. Finally, to achieve high selectivities and yields, fine-tuning of reaction conditions including solvent type, temperature, and optimal reaction times to be considered.

Amperometric assay of vitamin C using an ascorbate oxidase enzyme electrode

1979 ◽  
Vol 44 (11) ◽  
pp. 3395-3404 ◽  
Author(s):  
Pavel Posádka ◽  
Lumír Macholán
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Oxygen Electrode ◽  
Ascorbate Oxidase ◽  
Current Response ◽  
Reaction Conditions ◽  
Long Term Storage ◽  
Oxidase Enzyme ◽  
Optimal Reaction

An oxygen electrode of the Clark type, coated by a thin, active layer of chemically insolubilized ascorbate oxidase from squash peelings specifically detects by measuring oxygen uptake 10 to 400 μg of ascorbic acid in 3 ml of phosphate buffer. The record of current response to substrate addition lasts 1-2 min. The ascorbic acid values determined in various samples of fruit juices are in good agreement with the data obtained by titration and polarography. The suitable composition of the membrane and its lifetime and stability during long-term storage are described; optimal reaction conditions of vitamin C determination and the possibilities of interference of other compounds are also examined. Of the 35 phenols, aromatic amines and acids tested chlorogenic acid only can cause a positive error provided that the enzyme membrane has been prepared from ascorbate oxidase of high purity.

scholarly journals A Novel Pseudomonas geniculata AGE Family Epimerase/Isomerase and Its Application in d-Mannose Synthesis

Foods ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1809
Author(s):  
Zhanzhi Liu ◽  
Ying Li ◽  
Jing Wu ◽  
Sheng Chen
Keyword(s):  
3D Structure ◽  
Three Dimensional ◽  
Optimal Temperature ◽  
Reaction Conditions ◽  
Enzymatic Production ◽  
Physiological Properties ◽  
Potential Applications ◽  
Kinetics Of ◽  
Optimal Reaction ◽  
Gene Age

d-mannose has exhibited excellent physiological properties in the food, pharmaceutical, and feed industries. Therefore, emerging attention has been applied to enzymatic production of d-mannose due to its advantage over chemical synthesis. The gene age of N-acetyl-d-glucosamine 2-epimerase family epimerase/isomerase (AGEase) derived from Pseudomonas geniculata was amplified, and the recombinant P. geniculata AGEase was characterized. The optimal temperature and pH of P. geniculata AGEase were 60 °C and 7.5, respectively. The Km, kcat, and kcat/Km of P. geniculata AGEase for d-mannose were 49.2 ± 8.5 mM, 476.3 ± 4.0 s−1, and 9.7 ± 0.5 s−1·mM−1, respectively. The recombinant P. geniculata AGEase was classified into the YihS enzyme subfamily in the AGE enzyme family by analyzing its substrate specificity and active center of the three-dimensional (3D) structure. Further studies on the kinetics of different substrates showed that the P. geniculata AGEase belongs to the d-mannose isomerase of the YihS enzyme. The P. geniculata AGEase catalyzed the synthesis of d-mannose with d-fructose as a substrate, and the conversion rate was as high as 39.3% with the d-mannose yield of 78.6 g·L−1 under optimal reaction conditions of 200 g·L−1d-fructose and 2.5 U·mL−1P. geniculata AGEase. This novel P. geniculata AGEase has potential applications in the industrial production of d-mannose.

scholarly journals Phytochemical, Antioxidant and Toxicological Investigation of Morinda citrifolia L. Blossoms

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Shixin Deng ◽  
Brett J. West ◽  
'Afa K. Palu ◽  
C. Jarakae Jensen
Keyword(s):  
Antioxidant Activity ◽  
Iridoid Glycosides ◽  
Radical Scavenging ◽  
Morinda Citrifolia ◽  
Preliminary Evaluation ◽  
Medicinal Uses ◽  
Potential Applications ◽  
History Of ◽  
Toxicological Investigation ◽  
Phytochemical Components

Noni blossoms have a long history of medicinal uses in tropical areas. This study was conducted to investigate the major phytochemical components, toxicological properties, and antioxidant activity of noni blossoms. An HPLC-PDA method was developed and validated for the identification and quantification of major components. The major phytochemicals were iridoid glycosides, deacetylasperulosidic acid and asperulosidic acid, and flavonoids, quercetin-3-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside and kaempferol-3-O-α-L-rhamnopyranosyl-(1→6)-β-D-glucopyranoside, each present at 3.764, 3.576, 1.513, and 3.096 mg/g, respectively. The aqueous extract of noni blossoms, at 500 μg/mL, exhibited greater antioxidant activity in the 2,2-diphenylpicrylhydrazyl radical scavenging assay than green tea (88.11 ± 0.01% versus 76.60 ± 0.05%). A primary DNA damage test in E. coli PQ37 (SOS-chromotest) and a twenty-four hour brine shrimp toxicity test did not reveal any genotoxic or cytotoxic activity. These results provide a useful reference for the identification of noni blossoms as well as preliminary evaluation of safety and efficacy. Further evaluation of the potential applications of noni blossoms is warranted.

scholarly journals Conventional and Organic Honeys as a Source of Water- and Ethanol Soluble Molecules with Nutritional and Antioxidant Characteristics

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3746
Author(s):  
Magdalena Polak-Śliwińska ◽  
Małgorzata Tańska
Keyword(s):  
Antioxidant Activity ◽  
Immune System ◽  
Vitamin C ◽  
Geographical Area ◽  
Production Method ◽  
Climatic Conditions ◽  
Organic Production ◽  
Organoleptic Characteristics ◽  
Nutritional Characteristics ◽  
The Impact

The benefits of natural honeybee products (e.g., honey, royal jelly, beeswax, propolis, beevenom and pollen) to the immune system are remarkable, and many of them are involved in the induction of antibody production, maturation of immune cells and stimulation of the immune system. The type of plants in the geographical area, climatic conditions and production method have a significantly influence on the nutritional quality of honey. However, this variability can influence consumer liking by the sensory attributes of the product. The aim of this work was to compare the most popular honeys from Poland in terms of nutritional value, organoleptic properties and antioxidant activity. In the study, five varieties of honey (honeydew, forest, buckwheat, linden and dandelion) from conventional and organic production methods were tested. The nutritional characteristics of honey samples included acidity, content of water, sugars, vitamin C, HMF and phenolics (total and flavonoids), while honey color, taste, aroma and consistency were investigated in the organoleptic characteristics. The antioxidant activity was determined in water- and ethanol-soluble honey extracts using DPPH and ORAC tests. The results showed that organoleptic and nutritional characteristics of popular Polish honeys differ significantly in relation to plant source and production method. The significant effect of honey variety on the content of HMF, saccharose and phenolics, as well as acidity and antioxidant capacity were noted. The impact of variety and variety × production method interaction was significant in the case of the content of vitamin C, glucose and fructose. A visible difference of buckwheat and forest honeys from other samples was observed. The highest content of total phenolics with antioxidant activity based on the SET mechanism was found in buckwheat honeys, while forest honeys were richer in flavonoids.

scholarly journals Comparative Assessment of the Basic Chemical Composition and Antioxidant Activity of Stevia rebaudiana Bertoni Dried Leaves, Grown in Poland, Paraguay and Brazil—Preliminary Results

2021 ◽  
Vol 11 (8) ◽  
pp. 3634
Author(s):  
Teresa Leszczyńska ◽  
Barbara Piekło ◽  
Aneta Kopeć ◽  
Benno F. Zimmermann
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Vitamin C ◽  
Dietary Fiber ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Stevia Rebaudiana ◽  
Steviol Glycosides ◽  
Total Polyphenols ◽  
Stevia Rebaudiana Bertoni

This study compares the content of basic nutrients (proteins, fats, digestible carbohydrates, dietary fiber and ash), steviol glycosides, selected antioxidants (vitamin C, total polyphenols) and antioxidant activity in dried leaves of Stevia rebaudiana Bertoni cultivated in Poland, Paraguay and Brazil and available in the direct sale. The basic chemical composition was determined by standard AOAC (Association of Official Analytical Chemists) methods. Content of steviol glycosides was determined by the UHPLC-UV chromatographic method. Total polyphenols content was expressed as gallic acid equivalent (GAE) and catechins equivalent (CE). Antioxidant activity was measured as ABTS●+ free radical scavenging activity. Dried leaves of S. rebaudiana grown in Poland had significantly higher contents of dietary fiber, and lower protein and ash content, compared to those derived from Paraguay and Brazil. The former had, however, considerably higher contents of total steviol glycosides, stevioside and rebaudioside D, compared to the remaining two plants. In the Paraguay-derived dried leaves, the content of rebaudioside A, C, E and rubusoside was found to be significantly lower. Dried leaves of S. rebaudiana Bertoni, cultivated in Poland, contained substantially more vitamin C and a similar content of total polyphenols, compared to those from Brazil and Paraguay. The examined material from Brazil and Paraguay plantations showed similar antioxidant activity, while that obtained from Polish cultivation was characterized by a significantly lower value of this parameter.

Facile fractionation of bamboo hydrolysate and characterization of isolated lignin and lignin-carbohydrate complexes

Holzforschung ◽  
2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Xiaodi Wang ◽  
Yongchao Zhang ◽  
Luyao Wang ◽  
Xiaoju Wang ◽  
Qingxi Hou ◽  
...  
Keyword(s):  
Solvent Extraction ◽  
Organic Solvent ◽  
Extraction Process ◽  
Water Soluble ◽  
Organic Solvent Extraction ◽  
Glycoside Linkage ◽  
Potential Applications ◽  
Tremendous Amount ◽  
Main Components ◽  
Hemicellulosic Sugars

AbstractAn efficient separation technology for hydrolysates towards a full valorization of bamboo is still a tough challenge, especially regarding the lignin and lignin-carbohydrate complexes (LCCs). The present study aimed to develop a facile approach using organic solvent extraction for efficiently fractionating the main components of bamboo hydrolysates. The high-purity lignin with only a trace of carbohydrates was first obtained by precipitation of the bamboo hydrolysate. The water-soluble lignin (WSL) fraction was extracted in organic solvent through a three-stage organic solvent extraction process, and the hemicellulosic sugars with increased purity were also collected. Furthermore, a thorough characterization including various NMR techniques (31P, 13C, and 2D-HSQC), GPC, and GC-MS was conducted to the obtained lignin-rich-fractions. It was found that the WSL fraction contained abundant functional groups and tremendous amount of LCC structures. As compared to native LCC of bamboo, the WSL fraction exhibited more typical LCC linkages, i.e. phenyl glycoside linkage, which is the main type of chemical linkage between lignin and carbohydrate in both LCC samples. The results demonstrate that organic phase extraction is a highly efficient protocol for the fractionation of hydrolysate and the isolation of LCC-rich streams possessing great potential applications.

scholarly journals Multifunctional Activities of Gold Nanoparticles Biosynthesized Using Bacteria Isolated from Mining Areas

2021 ◽  
Vol 11 (8) ◽  
pp. 3670
Author(s):  
Chih-Yu Chen ◽  
Yung-Chu Chang ◽  
Teh-Hua Tsai ◽  
Man-Hai Liu ◽  
Ying-Chien Chung
Keyword(s):  
Gold Nanoparticles ◽  
Mining Area ◽  
Synthesis Process ◽  
Average Particle ◽  
Bifidobacterium Lactis ◽  
E Coli ◽  
Mining Areas ◽  
Skin Cells ◽  
Study Results ◽  
Antityrosinase Activity

Research on gold nanoparticles (AuNPs) has often focused on their physical, chemical, and crystalline characteristics. Commercial AuNPs have been applied in the diverse fields of biomedicine, catalysis, photovoltaics, and sensing. In this study, we explored the various activities of AuNPs to widen their applicability. This paper presents a simple and rapid synthesis process of AuNPs with bacteria isolated from a gold mining area. We also investigated the optimization of reaction parameters for AuNP synthesis. The study results revealed that among the isolated strains, Bifidobacterium lactis and Escherichia coli demonstrated the highest capabilities of AuNP synthesis. The optimal pH values for AuNP synthesis by B. lactis (BLAuNPs) and E. coli (ECAuNPs) were 5.0 for 72 h of incubation and 8.0 for 24 h of incubation. The average particle sizes of ECAuNPs and BLAuNPs were 4.2 and 5.6 nm, respectively. Furthermore, these biogenic AuNPs were found to be stable with no aggregation after 3 months of storage. BLAuNPs and ECAuNPs exhibited high levels of antimicrobial, antioxidant, photocatalytic, and antityrosinase activity. Moreover, they were noncytotoxic to skin cells even at 100% melanin inhibitory concentrations. Considering the demonstrated multifunctional activities of AuNPs, BLAuNPs and ECAuNPs have promising potential for commercialization.

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