scholarly journals Optimal probe concentration for the hybridization in the basedelectrochemical biosensor gold nanowire

2014 ◽  
Vol 17 (4) ◽  
pp. 27-35
Author(s):  
Tien Huu Cao ◽  
Thien Quang Nguyen ◽  
Linh Van Ha ◽  
Hieu Van Le ◽  
Hung Van Manh Le
Keyword(s):  
Electrode Surface ◽  
Surface Density ◽  
Gold Electrode ◽  
Dna Probe ◽  
Optimal Concentration ◽  
Dna Biosensors ◽  
Gold Nanowire ◽  
Probe Concentration ◽  
Hybridization Efficiency ◽  
Density Probe

The immobilisation of DNA probe on gold electrode surface with the optimal concentration is very important to develop of the DNA biosensors. In this study, we conducted an experiment that determined the optimal concentration of the probe attached the electrodes for the maximum hybridization efficiency. We have used a method that control the surface density of DNA probe by annealing probe modified by thiol and mercaptohexanol. Based on the linear relationship between the mol ratio and density probe, we control probe concentration in the sensor fabricating process as well as the molecular density of DNA probes on the electrode surface. The result of probe concentration 500 nM is optimal for hybidization with DNA target.

scholarly journals Optimization of probe immobilization by covalent links on gold nanowire based- electrochemical biosensor

2015 ◽  
Vol 18 (3) ◽  
pp. 135-144
Author(s):  
Tien Huu Cao ◽  
Linh Van Ha ◽  
Hieu Van Le
Keyword(s):  
Electrode Surface ◽  
Surface Density ◽  
Gold Electrode ◽  
Electrochemical Biosensor ◽  
Dna Probe ◽  
Molar Ratio ◽  
Dna Biosensors ◽  
Gold Nanowire ◽  
Molecular Density ◽  
Probe Concentration

The optimization of DNA probe immobilisation on gold electrode surface is very important to develop DNA biosensors. In this study, we conducted an experiment to determine the optimal concentration of probe attached on the electrodes and probe immobilization agent (mercaptohexanol) for maximum hybridization efficacy. We have used a method to control the surface density of DNA probe by annealing probe modified by thiol and mercaptohexanol. With linear relationship between molar ratio and surface density of probe, by controlling probe concentration in sensor fabricating process, we can determine the molecular density of DNA probes on electrode surface. The results show that probe concentration 500 nM and 1.5 mM mercaptohaxenol are optimal for hybidization with DNA target.

scholarly journals Kinetic studies of HRP adsorption on ds-DNA immobilized on gold electrode surface by EIS and SPR

2010 ◽  
Vol 21 (9) ◽  
pp. 1648-1655 ◽  
Author(s):  
Danielle C. M Ferreira ◽  
Renata K Mendes ◽  
Lauro T Kubota
Keyword(s):  
Electrode Surface ◽  
Gold Electrode ◽  
Kinetic Studies

Effect of surfactants and halide ions on the adsorption and oxidation of homocysteine at the gold electrode

RSC Advances ◽  
2016 ◽  
Vol 6 (55) ◽  
pp. 50315-50321
Author(s):  
Jianying Wang ◽  
Shangshang Zuo ◽  
Cui Lu ◽  
Yanbing Zu ◽  
Zuofeng Chen
Keyword(s):  
Electrode Surface ◽  
Gold Electrode ◽  
Halide Ions

This study demonstrates how adsorptive species including a series of surfactants and halide ions affect the adsorption of Hcy on the electrode surface, as well as how the change of Hcy adsorption affects the oxidation of Hcy.

Novel Protocol for Covalent Immobilization of Horseradish Peroxidase on Gold Electrode Surface

2009 ◽  
Vol 21 (6) ◽  
pp. 696-700 ◽  
Author(s):  
Abd-Elgawad Radi ◽  
Xavier Muñoz-Berbel ◽  
Montserrat Cortina-Puig ◽  
Jean-Louis Marty
Keyword(s):  
Horseradish Peroxidase ◽  
Electrode Surface ◽  
Gold Electrode ◽  
Covalent Immobilization

Investigation of Hybridization Efficiency of a Sequence-Orientated Coaxial DNA Probe Microarryed on Biochips Using Atomic Force Microscope

2013 ◽  
Vol 284-287 ◽  
pp. 315-319
Author(s):  
Jui Chuang Wu ◽  
Dan Kai Yang ◽  
Yane Shu Lin ◽  
Jun Yi Chen
Keyword(s):  
Atomic Force Microscope ◽  
Fluorescent Dyes ◽  
Laser Scanner ◽  
Dna Probe ◽  
The Other ◽  
Chip Surface ◽  
Surface Probe ◽  
Atomic Force ◽  
Glass Slides ◽  
Hybridization Efficiency

Two sequence-inversed probes were microarrayed on glass slides to study the hybridization efficiency with their DNA targets. A fluorescence laser scanner and an atomic force microscope (AFM) were utilized to investigate the efficiency in different hybridization cases and their corresponding depth changes on the chips. The sequences of two targets were designed to be fully complementary to their shared DNA probe in a coaxial stacking configuration. In other words, after the first DNA target is hybridized (pre-hybridizing) onto the probe, the second one is stacked onto the non-hybridized region of the same probe. The pre-hybridizing and the second DNA targets were distinguished by two distinct fluorescent dyes. The enhancement of the hybridization efficiency was investigated through the comparison between the stacking and individual hybridization configurations. AFM was used to measure the depths of two probes at different steps of hybridization. The results indicated that the depths increased as the hybridization proceeded. Probe#1, pre-hybridizing close to the chip surface, obtained a thicker depth than the other probe pre-hybridizing away from the chip surface, Probe#2. A hypothesis was proposed to explain how the depth variation was associated with the observed hybridization efficiency.

Characterization of Mercaptoethylamine-Modified Gold Electrode Surface and Analyses of Direct Electron Transfer to Putidaredoxin

Langmuir ◽  
1995 ◽  
Vol 11 (12) ◽  
pp. 4818-4822 ◽  
Author(s):  
Ling Sang Wong ◽  
Vincent L. Vilker ◽  
William T. Yap ◽  
Vytas Reipa
Keyword(s):  
Electron Transfer ◽  
Electrode Surface ◽  
Gold Electrode ◽  
Direct Electron Transfer

Electrochemical Control of CO/NO Ligand Exchange in a Triruthenium Cluster Monolayer Assembled on a Gold Electrode Surface

2004 ◽  
Vol 126 (24) ◽  
pp. 7434-7435 ◽  
Author(s):  
Shen Ye ◽  
Wei Zhou ◽  
Masaaki Abe ◽  
Takuma Nishida ◽  
Longfei Cui ◽  
...  
Keyword(s):  
Electrode Surface ◽  
Ligand Exchange ◽  
Gold Electrode ◽  
Electrochemical Control

An electrochemical sensing platform based on local repression of electrolyte diffusion for single-step, reagentless, sensitive detection of a sequence-specific DNA-binding protein

The Analyst ◽  
2014 ◽  
Vol 139 (9) ◽  
pp. 2193-2198 ◽  
Author(s):  
Yun Zhang ◽  
Fang Liu ◽  
Jinfang Nie ◽  
Fuyang Jiang ◽  
Caibin Zhou ◽  
...  
Keyword(s):  
Dna Binding ◽  
Electrode Surface ◽  
Electrochemical Biosensor ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Single Step ◽  
Dna Probe ◽  
Electrochemical Sensing ◽  
Sensing Platform ◽  
First Time

This paper describes for the first time an electrochemical biosensor, which employs a DNA probe modified with a redox tag close to electrode surface, for picomolar detection of a sequence-specific DNA-binding protein.

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