scholarly journals BIOACTIVITIES AND CHEMICAL CONSTITUENTS OF A VIETNAMESE MEDICINAL PLANT VANG SE JASMINUM SUBTRIPLINERVE BLUME

2012 ◽  
Vol 15 (3) ◽  
pp. 37-44
Author(s):  
Huong Thi Diem Nguyen ◽  
Son Hong Phan ◽  
Huong Dang Thien Bui ◽  
Hoai Thi Cam Ho ◽  
Mai Thi Thanh Nguyen
Keyword(s):  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Antioxidative Activity ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
2D Nmr ◽  
Dpph Radical ◽  
Dpph Radical Scavenging

From the total crude ethanol extract of Jasminum subtriplinerve Blume.’s leaves and stems, (Vang se in Vietnamese), four extracts were obtained by partitioning with petroleum ether, cloroform, ethyl acetate and n-butanol solvents. These four extracts were tested for antioxydative activity, determined using the DPPH●.radical scavenging and nitric oxyde-inhibitory assay. All the extracts showed antioxidative activity except the petroleum ether extracts. Among the crude extracts, the acetate ethyl extract was the most potent extract in both assays with the SC50 values of 8.2 μg/mL and 80.7 μg/mL, respectively. From the VS3 substract, three compounds were isolated, including two acids namely 3,4- dihidroxybenzoic acid (1), 3,4,5-trihidroxybenzoic acid (2) and a glycoside, verbascoside (3). The structure of those compounds was elucidated by spectrometric methods IR, MS, LC-MS, 1D-NMR, and 2D-NMR.

scholarly journals ANTIOXIDATIVE ACTIVITIES AND CHEMICAL CONSTITUENTS OF THE ETHYL ACETATE EXTRACT FROM JASMINUM UNDULATUM KER-GAWL

2011 ◽  
Vol 14 (2) ◽  
pp. 50-57
Author(s):  
Anh Thi Tran ◽  
Binh Thanh Nguyen ◽  
Hoai Thi Cam Ho ◽  
Huong Dang Thien Bui ◽  
Mai Thi Thanh Nguyen
Keyword(s):  
Nitric Oxide ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Antioxidative Activity ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Dpph Radical ◽  
Dpph Radical Scavenging

From the total crude ethanol extract of Jasminum undulatum Ker Gawl.’s leaves and stems, five fractionss were obtained by partitioning with petroleum ether, chloroform, ethyl acetate and n-butanol solvents. These five fractions were investigated for antioxidative activity using the DPPH radical scavenging and nitric oxide-inhibitory assay. All the fractions showed antioxidative activity except the petroleum ether fraction. Among the fractionss, the ethyl acetate fraction was the most potent fraction in both assays with the SC50 values of 5.30 μg/ml and 80.90 μg/ml, respectively. Further investigation on the eight sub-fractions isolated and extracted from the ethyl acetate fraction showed that one of these sub-fractions, the TE6 sub-fraction, showed the most significant antioxidative activity with the SC50 values of 3.15 μg/ml and 61.83 μg/ml respectively in the DPPH radical scavenging and nitric oxide-inhibitory assay. From the TE4 and TE6 sub-fractions, three compounds were isolated, including p-tyrosol (1), protocatechuic acid (2) and hydroxytyrosol (3). The structure of those compounds were elucidated by spectrometric methods IR, MS, 1D-NMR, and 2D-NMR.

scholarly journals Phytochemical screening and antioxidant activity of Melastoma malabathricum and Chromolaena odorata by DPPH radical scavenging method

Food Research ◽  
2021 ◽  
Vol 5 (S4) ◽  
pp. 30-37
Author(s):  
N.H. Ismail ◽  
Amira N.H. ◽  
S.N.H.M. Latip ◽  
W.Z.W.M. Zain ◽  
S.N.A. Aani ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Nutritive Value ◽  
Chemical Constituents ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Dpph Radical ◽  
Chromolaena Odorata ◽  
Melastoma Malabathricum ◽  
Dpph Radical Scavenging

Melastoma malabathricum and Chromolaena odorata are classified under broad-leaved weeds that are widely spread in the open land area. Melastoma malabathricum is commonly known as ‘‘senduduk’’, and C. odorata is locally known as “Pokok Kapal Terbang”. Both weeds are categorized as potential weeds as they have high nutritive value and are rich in chemical compounds. This study aimed to determine their chemical constituents and possible potential for antioxidant activity as these weeds have been reported to possess antioxidant properties. Screening of the plants was performed using standard methods and revealed the existence of various secondary metabolites such as saponins, terpenoids, phenols, tannins, and flavonoids of both weed extracts. Antioxidant activity was validated by the DPPH radical scavenging assay of M. malabathricum and C. odorata crude ethanol extract. The IC50 values for the percentage radical scavenging effects for the extracts were determined. The IC50 value of M. malabatrichum extract was 81.116 μg/mL, C. odorata was 312.903 μg/mL, Vitamin C was 31.023 μg/mL and BHA was 71.521 μg/mL respectively. The study showed that the antioxidant activity of M. malabatrichum was more potent and better than C. odorata.

Pharmacognostical fingerprinting and selective bioactivity studies of Solanum glaucophyllum Desf.

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Anindya Sundar Ray ◽  
Suman Kalyan Mandal ◽  
Chowdhury Habibur Rahaman
Keyword(s):  
Medicinal Plant ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Phytochemical Analysis ◽  
Antimicrobial Studies ◽  
Maximum Inhibition ◽  
Crude Drugs ◽  
Phytochemical Studies ◽  
Dpph Radical Scavenging

Solanum glaucophyllum Desf. (Solanaceae) is traditionally used for curing several health conditions in both human and domesticated animals. There is no data available regarding pharmacognostic standardization and pharmacological activity of this less known medicinal plant. Therefore, the aim of the present work is to prepare pharmacognostic fingerprints of crude drugs obtained from the leaf and stem parts of S. glaucophyllum. Apart from this detailed phytochemical analysis, antioxidant and antimicrobial studies have also been carried out. Pharmacognostic study revealed that the leaves are amphistomatic and stomata are strictly of anisocytic type. Values of stomatal index, palisade ratio, ash content of the investigated parts are found distinct and can be used as pharmacognostic standards for evaluation of crude drugs of this medicinal plant. Phytochemical studies of the leaf and stem parts indicated that phenolics, flavonoids, tannins and alkaloids are present in quite impressible amount. Curcumin content in stem was found very high through HPLC analysis. In DPPH radical scavenging assay, the stem part showed significant antioxidant potential. Ethanol extract of stem produced maximum inhibition zone (11mm) against Pseudomonas aeruginosa at the concentration of 50 mg/ml among five test microorganisms. Some pharmacognostic features recorded in this study as diagnostic ones will help in proper identification of this ethnomedicinal plant in its fresh as well as dried form. On the other hand preliminary phytochemical, antioxidant and antimicrobial studies highlight the S. glaucophyllum as a promising candidate for bioprospecting.

scholarly journals Anti-proliferative and immunomodulatory activities of fractions from methanol root extract of Abrus precatorius L

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Emeka E. Okoro ◽  
Omolaja R. Osoniyi ◽  
Almas Jabeen ◽  
Sidrah Shams ◽  
M. I. Choudhary ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Hela Cells ◽  
Oxidative Burst ◽  
Radical Scavenging ◽  
Root Extract ◽  
Dpph Radical ◽  
Abrus Precatorius ◽  
Tnf Α ◽  
Murine Fibroblast ◽  
Dpph Radical Scavenging

Abstract Background Abrus precatorius possesses various therapeutic properties including anticancer potentials. This study evaluated the anti-proliferative activities of fractions of methanol root extract of A. precatorius on breast and cervical cancer cells and their immunomodulatory effect. Phytochemical screening was done by FTIR and GCMS. In vitro anti-proliferative effect was evaluated on human breast cancer (AU565) and cervical cancer (HeLa) cells and on murine fibroblast (NIH 3 T3) cells. Antioxidant activity was performed via DPPH radical scavenging assay. The immunomodulatory potential of fractions was evaluated by inhibition of phagocytes oxidative burst (ROS), Nitric oxide (NO) and proinflammatory cytokine TNF-α. Results A. precatorius fractions showed different chemical groups and were somewhat selective in antiproliferative activity against studied cancer cells. Ethyl acetate fraction showed the most significant antiproliferative activity with IC50 values of 18.10 μg/mL and 11.89 μg/mL against AU565 and HeLa cells respectively. Hexane fraction significantly (p < 0.05) inhibited HeLa cells (IC50 18.24 ± 0.16 μg/mL), whereas aqueous fraction showed mild inhibition (IC50 46.46 ± 0.14 μg/mL) on AU565 cell proliferation. All fractions showed no cytotoxicity against NIH-3 T3 murine fibroblast normal cells. All fractions showed potent and significant (p < 0.001) DPPH radical scavenging activity as well as suppressed phagocytic oxidative burst. Hexane (< 1 μg/mL), ethyl acetate (< 1 μg/mL), and butanol (5.74 μg/mL) fractions potently inhibited the cytokine TNF- α, hexane (< 1 μg/mL) and ethyl acetate (< 1 μg/mL) fractions also potently inhibited NO. Conclusions The antiproliferative activities and suppressive effect on the phagocytic oxidative burst, NO and proinflammatory cytokine might be due to the synergistic actions of bioactive compounds especially flavonoids present in the assayed fractions and therefore, suggest chemotherapeutic use of A. precatorius in cancer treatment.

scholarly journals Chemical and Biological Investigation of Some Secondary Metabolites in Atriplex Halimus Growing in Egypt

2012 ◽  
Vol 7 (11) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Amal Kabbash ◽  
Nagwa Shoeib
Keyword(s):  
Multidrug Resistance ◽  
Soluble Fraction ◽  
Radical Scavenging ◽  
2D Nmr ◽  
Flavonoid Glycosides ◽  
Dpph Radical ◽  
Biological Investigation ◽  
Atriplex Halimus ◽  
Aerial Parts ◽  
Dpph Radical Scavenging

The n-BuOH-soluble fraction of the MeOH-CH2Cl2 (1:1) extract of the aerial parts of Egyptian Atriplex halimus L. yielded two new flavonol glycosides, designated as atriplexoside A (1) [3′- O-methylquercetin-4′- O-β-apiofuranoside-3- O-(6″- O-α-rhamnopyranosyl-β-glucopyranoside)] and atriplexoside B (2) [3′- O-methylquercetin-4′- O-(5″″- O-β-xylopyranosyl-β-apiofuranoside)-3- O-(6″- O-α-rhamnopyranosyl-β-glucopyranoside)], together with six known compounds: two phenolic glucosides (3, 4), one ecdysteroid (5), one megastigmane (6) and two methoxylated flavonoid glycosides (7, 8). The structures of the compounds were elucidated by detailed spectroscopic analysis, including HR-ESI-MS and 2D-NMR spectroscopic data. DPPH radical scavenging, antileishmanial and anti-multidrug resistance activities were investigated using the n-BuOH-soluble fraction as well as the isolated compounds. Compound 8 (5- O-methylquercetin-3- O-(6″- O-α-rhamnopyranosyl-β-glucopyranoside) presented marked DPPH radical scavenging, weak antileishmanial and anti-multidrug resistance activity while the other tested compounds showed weaker activities.

scholarly journals ANTIOXIDANT ACTIVITIES OF FRACTIONS FROM ETHYL ACETATE EXTRACTS OF GARCINIA FRUTICOSA LAUTERB. LEAVES

2018 ◽  
Vol 10 (1) ◽  
pp. 44 ◽  
Author(s):  
Riza Shabrina ◽  
Berna Elya ◽  
Arikadia Noviani
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Group Identification ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Dpph Radical Scavenging

Objective: This study aimed to fractionate the antioxidant activity of the ethyl acetate leaf extract and to characterize the most active fractionsaccording to compound groups.Methods: The ethyl acetate extract was fractionated with column chromatography using a gradient elution system. Fractions were first screenedqualitatively for antioxidant activity before active fractions were quantified with respect to in vitro antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and the ferric-reducing antioxidant power (FRAP) assay. The compound groups were identifiedfollowing separation by thin-layer chromatography.Results: Fraction 11 exhibited the greatest DPPH radical-scavenging activity, with an IC50 value of 6.58 μg/mL, while the fraction with the greatestantioxidant activity according to the FRAP assay was fraction 10, with a ferric ion equivalent antioxidant activity value of 1015.34 μmol/g.Conclusion: Compound group identification revealed that Fractions 10 and 11 contained flavonoids, with two common to both fractions, whilefraction 10 also contained one specific flavonoid.

scholarly journals Determination of Antioxidant Activity and Total Tannin from Drumstick (Moringa oleifera Lam.) Leaves Using Different Solvent Extraction Methods

2020 ◽  
Vol 8 (12) ◽  
pp. 2749-2755
Author(s):  
Mohammad Afzal Hossain ◽  
Nadia Khan Disha ◽  
Jahid Hasan Shourove ◽  
Pappu Dey
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Tannin Content ◽  
Dpph Radical Scavenging Activity ◽  
Dpph Radical Scavenging

This study was undertaken to estimate the antioxidant activity and total tannin content of Moringa olifera Lam. (Moringa) leaves and find a suitable extraction condition for maximum yield. Two types of solvent and three different extraction times and temperatures were experimented for extraction. Moringa leaves were oven-dried and powdered to determine the antioxidant activity in terms of total phenolic content (TPC), ferric reducing antioxidant power (FRAP), and DPPH radical scavenging activity and total tannin content. The maximum TPC value noted in methanol extract, and it ranges from 6.46 ± 0.34 g to 3.91±0.19 g gallic acid equivalent (GAE)/100g dry sample (DM). The FRAP values varied from 211.6 ± 3.75 to 344.13 ± 3.26 mg ascorbic acid equivalent (AAE)/100g DM and 166.67 ± 2.90 to 224.93 ± 1.94 mg AAE/100g DM, for methanol and ethanol extract, respectively. The DPPH radical scavenging activity ranged from 46.32 ± 1.07 to 58.09 ± 0.92% for methanol extract and 56.76 ± 1.48% to 69.72 ± 1.15% for ethanol extract. The total tannin content varied from 6.84 ± 0.05 to 10.22 ± 1.11 mg GAE/100 g dry sample and 6.77 ± 0.08 to 9.23 ± 0.51 mg GAE/ 100g DMfor methanol and ethanol extract, respectively. The highest antioxidant yield for methanol and ethanol extract of Moringa leaves differed for various conditions. Overall, Moringa leaves showed excellent antioxidant properties that can be commercially and domestically used.

scholarly journals COMPARATIVE ANALYSIS OF PHYTOCHEMICAL, ANTIBACTERIAL, AND ANTIOXIDANT ACTIVITY OF DIFFERENT EXTRACTS OF AZADIRACHTA INDICA LEAVES

2019 ◽  
pp. 151-154
Author(s):  
GAURAV SHARMA ◽  
ANKITA THAKUR ◽  
SOHAN LAL ◽  
ROHIT KUMAR NADDA
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Azadirachta Indica ◽  
Comparative Evaluation ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Dpph Radical ◽  
Bacterial Strains ◽  
Dpph Radical Scavenging ◽  
Radical Scavenging Assay

Objective: The objective of the present study was the analysis of phytochemicals in various extracts of Azadirachta indica leaves, comparative evaluation of antibacterial activity of the various extracts of A. indica leaves against Escherichia coli and Staphylococcus aureus, and comparative evaluation of antioxidant activity in various extracts of A. indica leaves using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. Methods: Various extracts were prepared by crushing the samples. Antibacterial susceptibility test, various phytochemical tests for qualitative analysis, and DPPH radical scavenging assay for antioxidant activity were performed. Results: The result suggested that alkaloids, flavonoids, and terpenoids were present in all the four extracts. Tannins were absent in the ethyl acetate extract, and phenols were only present in the ethyl acetate extract. Sterols and phlobatannins were absent in all the four extracts. Saponins were only present in the aqueous extract, and amino acids were only present in the ethyl acetate extract. The bacterial strains S. aureus and E. coli were used against the different extracts of A. indica leaves, i.e., methanol, chloroform, ethyl acetate, and aqueous. Conclusion: The results suggested that bioactive compounds found in leaves of A. indica contribute to its pharmacological activities.

scholarly journals IN VITRO SCREENING OF ANTIOXIDANT AND ANTIAGING POTENTIAL OF CUCUMIS SATIVUS FRUIT EXTRACT

2020 ◽  
pp. 187-190
Author(s):  
CHANCHAL GARG ◽  
RAVINDER SINGH ◽  
MUNISH GARG
Keyword(s):  
Ethyl Acetate ◽  
Cucumis Sativus ◽  
Inhibitory Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
Positive Control ◽  
Dpph Radical ◽  
Dpph Radical Scavenging

Objective: The present study was designed to screen the anti-aging and anti-wrinkle potential of Cucumis sativus fruit through in vitro estimation of antioxidant, anti-hyaluronidase, anti-elastase, anti-collagenase/anti-matrix metalloproteinase (MMP)-1, and anti-tyrosinase activity. Methods: Raw juice of cucumber was taken, filtered and fractionated with ethyl acetate and n-butanol. The obtained extracts were then evaluated for their antioxidant potential through 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay taking ascorbic acid as positive control and other enzymatic activities in reference to hyaluronidase inhibition, MMP-1/collagenase inhibition, and elastase inhibition taking catechin as reference standard whereas for tyrosinase inhibition the standard used was quercetin. Results: All the evaluations were performed in triplicates and results were noted down. It was observed that aqueous extract of C. sativus fruits showed a maximum DPPH radical scavenging activity (p<0.0001), half-maximal inhibitory concentration (IC50) at a concentration of 122.67 μg/ml. The ethyl acetate fraction of C. sativus fruits exhibited maximum hyaluronidase (p<0.0001), MMP-1/collagenase (p<0.04), and tyrosinase (p<0.04) inhibitory activity, IC50 at a concentration of 59.54, 45.79, and 24.46 μg/ml, respectively. The elastase (p<0.0001) inhibitory activity by n-butanol fraction of C. sativus fruits extract was maximum, IC50 at a concentration of 52.76 μg/ml. Conclusion: A potent anti-aging and anti-wrinkle properties were well demonstrated by C. sativus, as depicted from the results obtained.

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