Genetic engineering of soybean using candidate genes to improve drought tolerance

2009 ◽  
Author(s):  
Satish Kumar Guttikonda
Keyword(s):  
Genetic Engineering ◽  
Drought Tolerance ◽  
Candidate Genes

scholarly journals Transcriptomic analysis of sweet potato under dehydration stress identifies candidate genes for drought tolerance

Plant Direct ◽  
2018 ◽  
Vol 2 (10) ◽  
pp. e00092 ◽  
Author(s):  
Kin H. Lau ◽  
María del Rosario Herrera ◽  
Emily Crisovan ◽  
Shan Wu ◽  
Zhangjun Fei ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Candidate Genes ◽  
Sweet Potato ◽  
Transcriptomic Analysis ◽  
Dehydration Stress

scholarly journals Allele mining and haplotype discovery in barley candidate genes for drought tolerance

Euphytica ◽  
2011 ◽  
Vol 181 (3) ◽  
pp. 341-356 ◽  
Author(s):  
András Cseri ◽  
Mátyás Cserháti ◽  
Maria von Korff ◽  
Bettina Nagy ◽  
Gábor V. Horváth ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Candidate Genes ◽  
Allele Mining

scholarly journals Integration of gene-based markers in a pearl millet genetic map for identification of candidate genes underlying drought tolerance quantitative trait loci

2012 ◽  
Vol 12 (1) ◽  
pp. 9 ◽  
Author(s):  
Deepmala Sehgal ◽  
Vengaldas Rajaram ◽  
Ian Peter Armstead ◽  
Vincent Vadez ◽  
Yash Pal Yadav ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Drought Tolerance ◽  
Candidate Genes ◽  
Pearl Millet ◽  
Genetic Map ◽  
Quantitative Trait ◽  
Trait Loci ◽  
Gene Based Markers

scholarly journals Identification of candidate genes for drought tolerance in coffee by high-throughput sequencing in the shoot apex of different Coffea arabica cultivars

2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Luciana Souto Mofatto ◽  
Fernanda de Araújo Carneiro ◽  
Natalia Gomes Vieira ◽  
Karoline Estefani Duarte ◽  
Ramon Oliveira Vidal ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Candidate Genes ◽  
High Throughput ◽  
Shoot Apex ◽  
Coffea Arabica ◽  
High Throughput Sequencing

scholarly journals Identification and Confirmation of Loci Associated With Canopy Wilting in Soybean Using Genome-Wide Association Mapping

2021 ◽  
Vol 12 ◽  
Author(s):  
Siva K. Chamarthi ◽  
Avjinder S. Kaler ◽  
Hussein Abdel-Haleem ◽  
Felix B. Fritschi ◽  
Jason D. Gillman ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Association Mapping ◽  
Candidate Genes ◽  
Stress Responses ◽  
Water Conservation ◽  
Production Systems ◽  
Block Design ◽  
Plant Stress ◽  
Significant Snps ◽  
Nucleotide Polymorphisms

Drought causes significant soybean [Glycine max (L.) Merr.] yield losses each year in rain-fed production systems of many regions. Genetic improvement of soybean for drought tolerance is a cost-effective approach to stabilize yield under rain-fed management. The objectives of this study were to confirm previously reported soybean loci and to identify novel loci associated with canopy wilting (CW) using a panel of 200 diverse maturity group (MG) IV accessions. These 200 accessions along with six checks were planted at six site-years using an augmented incomplete block design with three replications under irrigated and rain-fed treatments. Association mapping, using 34,680 single nucleotide polymorphisms (SNPs), identified 188 significant SNPs associated with CW that likely tagged 152 loci. This includes 87 SNPs coincident with previous studies that likely tagged 68 loci and 101 novel SNPs that likely tagged 84 loci. We also determined the ability of genomic estimated breeding values (GEBVs) from previous research studies to predict CW in different genotypes and environments. A positive relationship (P ≤ 0.05;0.37 ≤ r ≤ 0.5) was found between observed CW and GEBVs. In the vicinity of 188 significant SNPs, 183 candidate genes were identified for both coincident SNPs and novel SNPs. Among these 183 candidate genes, 57 SNPs were present within genes coding for proteins with biological functions involved in plant stress responses. These genes may be directly or indirectly associated with transpiration or water conservation. The confirmed genomic regions may be an important resource for pyramiding favorable alleles and, as candidates for genomic selection, enhancing soybean drought tolerance.

scholarly journals Investigation of genes involved in somatic embryogenesis and plant-Agrobacterium interactions through transcriptional profiling

2021 ◽  
Author(s):  
◽  
Christopher Willig
Keyword(s):  
Somatic Embryogenesis ◽  
Genetic Engineering ◽  
Candidate Genes ◽  
Dna Sequences ◽  
Zygotic Embryo ◽  
Transcriptional Profiling ◽  
Differential Expression Analysis ◽  
Genetic Material ◽  
Plant Cells ◽  
Plant Genes

Plant genetic engineering relies on the ability to transmit and express cloned DNA sequences in plant cells (transformation) as well as the capacity for the cells carrying this DNA to undergo division and differentiation (regeneration), eventually giving rise to a mature whole plant. The breadth of application for genetic engineering is limited by constraints on one or both of these factors in many plant species and individual varieties. Uncovering plant genes which are involved in important aspects of either component can inform the development of technologies that serve to enable or improve the efficiency of genetic modification methods. The most commonly employed method of delivering exogenous genetic material into plant cells is via disarmed strains of the plant pathogen Agrobacterium tumefaciens. Somatic embryogenesis is a frequently applied mode of plant regeneration following DNA delivery, especially in major cereal crops such as maize, rice, and sorghum. In the work reported here, whole transcriptome sequencing (RNA-seq) was used in two different experiments to capture transcriptional dynamics throughout early somatic embryogenesis in immature zygotic embryo tissue of the major crop plant sorghum (Sorghum bicolor), and throughout early times following host plant inoculation with A. tumefaciens in seedlings of the model plant Arabidopsis thaliana (Chapters 2 and 3, respectively). In both cases, differential expression analysis revealed many genes which were induced either during somatic embryogenesis or in response to inoculation with either virulent or avirulent A. tumefaciens strains. Several of these genes were highlighted as candidates for future study into their potential role in the respective processes. Multiple candidate genes were functionally tested, using transgenic methods, for the possibility of having a role in the regulation of somatic embryogenesis in sorghum (Chapter 4). These experiments failed to confirm an influence over the process for all candidate genes that were evaluated. The experimental work documented herein contributes to a growing body of literature documenting plant genes which could serve as possible targets for techniques that work to enhance the utility of biotechnological methods for improving traits in plants.

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