scholarly journals Novel PCR-based rapid detection strategies for Escherichia coli O157 :H7 and Salmonella in meat products

2009 ◽  
Author(s):  
Luxin Wang
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Meat Products ◽  
Escherichia Coli O157 ◽  
Detection Strategies

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

Development of PCR assays for detection of Escherichia coli O157:H7 in meat products

Meat Science ◽  
2011 ◽  
Vol 88 (4) ◽  
pp. 767-773 ◽  
Author(s):  
Rubén Gordillo ◽  
Juan J. Córdoba ◽  
María J. Andrade ◽  
M. Isabel Luque ◽  
Mar Rodríguez
Keyword(s):  
Escherichia Coli ◽  
Meat Products ◽  
Escherichia Coli O157 ◽  
Pcr Assays

scholarly journals Escherichia coli O157:H7 outbreak linked to salami, British Columbia, Canada, 1999

2004 ◽  
Vol 132 (2) ◽  
pp. 283-289 ◽  
Author(s):  
D. M. MacDONALD ◽  
M. FYFE ◽  
A. PACCAGNELLA ◽  
A. TRINIDAD ◽  
K. LOUIE ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Case Control Study ◽  
Meat Products ◽  
Case Control ◽  
Pfge Pattern ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Matched Case ◽  
Matched Case Control Study ◽  
Control Study

An outbreak of E. coli O157:H7 infections was identified in November 1999 with a fivefold increase in the occurrence of laboratory-confirmed cases of E. coli O157:H7 infection. A matched case-control study was conducted. Samples of food from cases and from retailers were analysed for the presence of E. coli O157:H7. A total of 143 cases were identified over a 12-week period with the same pulsed-field gel electrophoresis (PFGE) pattern. The case-control study found that Company A salami was significantly associated with illness (Mantel–Haenszel matched odds ratio 10·0, 95% CI 1·4–434, P=0·01). Company A salami tested positive for E. coli O157:H7 and isolates had the same PFGE pattern as case isolates. An immediate voluntary national recall of Company A dry fermented meat products took place. Findings from the investigation of this outbreak suggest that the hold-and-test option may not be adequate to prevent shiga-toxigenic Escherichia coli (STEC) infection in salami consumers.

scholarly journals B cells Using Calcium Signaling for Specific and Rapid Detection of Escherichia coli O157:H7

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Ling Wang ◽  
Ronghui Wang ◽  
Byung-Whi Kong ◽  
Sha Jin ◽  
Kaiming Ye ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
B Cells ◽  
Calcium Signaling ◽  
Rapid Detection ◽  
Escherichia Coli O157

Rapid detection of Escherichia coli O157:H7 spiked into food matrices

2007 ◽  
Vol 584 (1) ◽  
pp. 66-71 ◽  
Author(s):  
Lisa C. Shriver-Lake ◽  
Stephanie Turner ◽  
Chris R. Taitt
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Escherichia Coli O157 ◽  
Food Matrices
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