Development of a post-traumatic osteoarthritis model to evaluate the effects of impact velocity and maximum strain on articular cartilage cell viability, matrix biomarkers, and material properties

2009 ◽  
Author(s):  
Nicole Poythress Waters
Keyword(s):  
Articular Cartilage ◽  
Cell Viability ◽  
Impact Velocity ◽  
Material Properties ◽  
Maximum Strain ◽  
Cartilage Cell ◽  
Post Traumatic ◽  
Articular Cartilage Cell

Development of Immortalized Human Articular Cartilage Cell Lines

1993 ◽  
pp. 267-272 ◽  
Author(s):  
G. Verbruggen ◽  
A. M. Malfait ◽  
K. F. Almgvist ◽  
E. M. Veys ◽  
S. Thenet ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Cell Lines ◽  
Human Articular Cartilage ◽  
Cartilage Cell ◽  
Articular Cartilage Cell

Panax ginseng C.A. Meyer modulates the levels of MMP3 in S12 murine articular cartilage cell line

2009 ◽  
Vol 124 (3) ◽  
pp. 397-403 ◽  
Author(s):  
Joon-Shik Shin ◽  
Namhee Park ◽  
Jehyeon Ra ◽  
Yangseok Kim ◽  
Minkyu Shin ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Cell Line ◽  
Panax Ginseng ◽  
Cartilage Cell ◽  
Articular Cartilage Cell

Immortalized mouse articular cartilage cell lines retain chondrocyte phenotype and respond to both anabolic factor BMP-2 and pro-inflammatory factor IL-1

2008 ◽  
Vol 215 (1) ◽  
pp. 68-76 ◽  
Author(s):  
Manas K. Majumdar ◽  
Priya S. Chockalingam ◽  
Ramesh A. Bhat ◽  
Richard Sheldon ◽  
Cristin Keohan ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Cell Lines ◽  
Inflammatory Factor ◽  
Cartilage Cell ◽  
Chondrocyte Phenotype ◽  
Articular Cartilage Cell

Biomedical Applications of Hemicellulose-Based Hydrogels

2020 ◽  
Vol 27 (28) ◽  
pp. 4647-4659 ◽  
Author(s):  
Haitang Liu ◽  
Ting Chen ◽  
Cuihua Dong ◽  
Xuejun Pan
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Articular Cartilage ◽  
Wound Dressing ◽  
Biomedical Applications ◽  
Cell Immobilization ◽  
Original Structure ◽  
Cartilage Cell ◽  
Artificial Plasma ◽  
Articular Cartilage Cell

Background: Hydrogel has a three-dimensional network structure that is able to absorb a large amount of water/liquid and maintain its original structure. Hemicellulose (HC) is the second most abundant polysaccharide after cellulose in plants and a heterogeneous polysaccharide consisting of various saccharide units. The unique physical and chemical properties of hemicellulose make it a promising material for hydrogels. Methods: This review first summarizes the three research hotspots on the hemicellulose-based hydrogels: intelligence, biodegradability and biocompatibility. It also overviews the progress in the fabrication and applications of hemicellulose hydrogels in the drug delivery system and tissue engineering (articular cartilage, cell immobilization, and wound dressing). Results: Hemicellulose-based hydrogels have many unique properties, such as stimuliresponsibility, biodegradability and biocompatibility. Interpenetrating networking can endow appropriate mechanical properties to hydrogels. These properties make the hemicellulose-based hydrogels promising materials in biomedical applications such as drug delivery systems and tissue engineering (articular cartilage, cell immobilization, and wound dressing). Conclusion: Hydrogels have been widely used in biomedicine and tissue engineering areas, such as tissue fillers, drug release agents, enzyme encapsulation, protein electrophoresis, contact lenses, artificial plasma, artificial skin, and tissue engineering scaffold materials. This article reviews the recent progress in the fabrication and applications of hemicellulose-based hydrogels in the biomedical field.

scholarly journals Hh pathway expression in the blood, synovial cells and chondrocytes of different rheumatoid arthritis models

2021 ◽  
Vol 18 (3) ◽  
pp. 499-504
Author(s):  
Yingyi Wu ◽  
Guangxia Yang ◽  
Jing Fei ◽  
Yang Huang
Keyword(s):  
Rheumatoid Arthritis ◽  
Cell Proliferation ◽  
Articular Cartilage ◽  
Protein Expression ◽  
Cell Apoptosis ◽  
Fluorescein Isothiocyanate ◽  
Control Group ◽  
Cartilage Cell ◽  
Expression Levels ◽  
Articular Cartilage Cell

Purpose: To investigate the effect of the hedgehog (Hh) pathway inhibitor, cyclopamine, and activator purmorphamine on articular cartilage cell proliferation. Methods: Rats were subjected to AA and CIA models. Secondary paw swelling was measured at 12, 15, 18, 21, 24, 27, and 30 days. The rats were sacrificed on day 30. Tissues from the cartilage and knee joints were collected. Cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay while cell apoptosis was determined by annexin V-fluorescein isothiocyanate/propidium iodide assay. Protein expression levels of Shh, Ptch1 and Gli1 were determined by Western blotting. Results: Compared with the control group, arthritis index and secondary foot swelling of the adjuvant arthritis (AA) and collagen-induced arthritis (CIA) groups deteriorated significantly (p < 0.05). MTT data revealed that cyclopamine promoted articular cartilage cell proliferation of the AA and CIA groups. The cell proliferation rates of AA and CIA groups were significantly higher than that of control group (p < 0.05). Flow cytometry showed that the cell apoptosis rates of AA and CIA groups were significantly lower than that of control group (p < 0.05). Compared with control, cyclopamine decreased the protein expression levels of sonic Hh, patched homologue 1 and glioma-associated oncogene homologue, but the effect of purmorphamine was the reverse. Conclusion: Hh pathway inhibitor (cyclopamine) and activator (purmorphamine) affect the expression of Hh pathway. Disruption of the Hh pathway may be of potential therapeutic significance in protecting articular cartilage from rheumatoid arthritis.

scholarly journals Genetic Deletion of Interleukin-15 Is Not Associated with Major Structural Changes Following Experimental Post-Traumatic Knee Osteoarthritis in Rats

2021 ◽  
Vol 11 (15) ◽  
pp. 7118
Author(s):  
Ermina Hadzic ◽  
Garth Blackler ◽  
Holly Dupuis ◽  
Stephen James Renaud ◽  
Christopher Thomas Appleton ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Subchondral Bone ◽  
Structural Changes ◽  
Cartilage Damage ◽  
Wild Type ◽  
Significant Difference ◽  
Post Traumatic ◽  
Interleukin 15 ◽  
Joint Trauma ◽  
Surgically Induced

Post-traumatic osteoarthritis (PTOA) is a degenerative joint disease, leading to articular cartilage breakdown, osteophyte formation, and synovitis, caused by an initial joint trauma. Pro-inflammatory cytokines increase catabolic activity and may perpetuate inflammation following joint trauma. Interleukin-15 (IL-15), a pro-inflammatory cytokine, is increased in OA patients, although its roles in PTOA pathophysiology are not well characterized. Here, we utilized Il15 deficient rats to examine the role of IL-15 in PTOA pathogenesis in an injury-induced model. OA was surgically induced in Il15 deficient Holtzman Sprague-Dawley rats and control wild-type rats to compare PTOA progression. Semi-quantitative scoring of the articular cartilage, subchondral bone, osteophyte size, and synovium was performed by two blinded observers. There was no significant difference between Il15 deficient rats and wild-type rats following PTOA-induction across articular cartilage damage, subchondral bone damage, and osteophyte scoring. Similarly, synovitis scoring across six parameters found no significant difference between genetic variants. Overall, IL-15 does not appear to play a key role in the development of structural changes in this surgically-induced rat model of PTOA.

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