scholarly journals Proteomic identification of the differentially expressed and phosphorylated proteins in 20-hydroxyecdysone (20E) signal transduction pathway in salivary gland of Drosophila melanogaster

2010 ◽  
Author(s):  
◽  
Yaning Sun
Keyword(s):  
Signal Transduction ◽  
Drosophila Melanogaster ◽  
Salivary Gland ◽  
Intracellular Localization ◽  
Signal Transduction Pathway ◽  
Phosphorylated Proteins ◽  
Rnai Technology ◽  
Pkc Isoforms ◽  
First Time

Protein kinase C (PKC) plays important role in 20-hydroxyecdysone (20E) signal transduction, however, little is known about the exact role of PKC in this process. In my research, PKC-regulated phosphorylation in 20E signal transduction is investigated in the salivary gland of Drosophila melanogaster. Our experiments demonstrate that PKCregulated phosphorylation is responsible for the intracellular localization of ecdysone receptor (EcR) and its heterodimeric partner, ultraspiracle protein (USP), which is possibly due to the forming of receptor complex with chaperons. We also confirmed PKC-regulated phosphorylation is required in 20E induced protein expression and identified 14 proteins induced by 20E but inhibited by PKC inhibitor. Using 2D Western blot and phospho-(Ser) PKC substrate, we were able to identify four phosphorylated PKC substrates in 20E signal transduction process, which may function in 20E-induced gene transcription/translation process or in ecdysteroid transporting. In addition, PKC isoforms in the salivary gland were also investigated by RNA interference (RNAi). For the first time, we showed the successful application of RNAi technology by soaking the salivary glands of D. melanogaster with dsRNAs.

Signal transduction pathway and physiological role of endothelin in the kidney

1994 ◽  
Vol 1 ◽  
pp. 80
Author(s):  
K. Tomita ◽  
A. Owada ◽  
H. Nonoguchi ◽  
Y. Terada ◽  
F. Marumo
Keyword(s):  
Signal Transduction ◽  
Signal Transduction Pathway ◽  
Physiological Role ◽  
Transduction Pathway

Stoichiometry of G protein subunits affects the Saccharomyces cerevisiae mating pheromone signal transduction pathway

1990 ◽  
Vol 10 (2) ◽  
pp. 510-517
Author(s):  
G M Cole ◽  
D E Stone ◽  
S I Reed
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Signal Transduction ◽  
G Protein ◽  
Signal Transduction Pathway ◽  
Mating Pheromone ◽  
Gal1 Promoter ◽  
Gamma Subunits ◽  
Adaptation Response ◽  
High Level

The Saccharomyces cerevisiae GPA1, STE4, and STE18 genes encode products homologous to mammalian G-protein alpha, beta, and gamma subunits, respectively. All three genes function in the transduction of the signal generated by mating pheromone in haploid cells. To characterize more completely the role of these genes in mating, we have conditionally overexpressed GPA1, STE4, and STE18, using the galactose-inducible GAL1 promoter. Overexpression of STE4 alone, or STE4 together with STE18, generated a response in haploid cells suggestive of pheromone signal transduction: arrest in G1 of the cell cycle, formation of cellular projections, and induction of the pheromone-inducible transcript FUS1 25- to 70-fold. High-level STE18 expression alone had none of these effects, nor did overexpression of STE4 in a MATa/alpha diploid. However, STE18 was essential for the response, since overexpression of STE4 was unable to activate a response in a ste18 null strain. GPA1 hyperexpression suppressed the phenotype of STE4 overexpression. In addition, cells that overexpressed GPA1 were more resistant to pheromone and recovered more quickly from pheromone than did wild-type cells, which suggests that GPA1 may function in an adaptation response to pheromone.

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