scholarly journals Importance of the PN-EN 16615: 2015 standard in the assessment of bactericidal activity of disinfectants intended for soaking wipes in medical area

2018 ◽  
pp. 185-190
Author(s):  
Agnieszka Chojecka ◽  
Patryk Tarka ◽  
Krzysztof Kanecki ◽  
Aneta Nitsch-Osuch
Keyword(s):  
Staphylococcus Aureus ◽  
Contact Time ◽  
Cross Contamination ◽  
Albumin Solution ◽  
Limit Values ◽  
Bovine Albumin ◽  
Medical Area ◽  
Test Product ◽  
Porous Surfaces

Introduction: The PN-EN 16615: 2015 standard is intended to determine the acting parameters of disinfectants used for surfaces disinfection with the wiping technique using wipes. It takes into account the application method of disinfectants on the non porous surfaces to be disinfected. The aim of the study was to assess the compliance of the obtained results of control tests with the limit values set in the PN-EN 16615: 2015 standard and to determine the disinfection parameters of the tested product against Staphylococcus aureus. Method: The disinfectant product tests were carried out using standard wipes described in PN-EN 16615: 2015 standard in four contact times of 1, 5, 10, 15 minutes under clean conditions: 0,3 g / l of bovine albumin solution. Results: Obtained results of control and validation of the dilution-neutralization method met the criteria of limit values of the PN-EN 16615: 2015 standard. The test product was active against Staphylococcus aureus within 15 minutes contact time under clean conditions (reduction > 5,59 lg). Conclusions: PN-EN 16615: 2015 standard ensures proper determination of acting parameters of disinfectants intended for use in the form of wipes, what contributes to effective disinfection of the surfaces, especially high-touch surfaces, and prevents the occurrence of cross-contamination of other surfaces in the medical area.

scholarly journals Evaluation of survival of Candida albicans on PVC surfaces in the test of yeasticidal activity of disinfectant

2020 ◽  
pp. 47-53
Author(s):  
Agnieszka Chojecka
Keyword(s):  
Candida Albicans ◽  
Contact Time ◽  
Pathogenic Microorganism ◽  
Drying Process ◽  
Suspension Density ◽  
Bovine Albumin ◽  
Disinfection Methods ◽  
The Difference ◽  
Drying Conditions

Introduction: Candida albicans survival tests on PCV carriers are necessary for the proper determination of the effectiveness yeasticidal activity of disinfectants. C. albicans is a pathogenic microorganism that causes fungal diseases and therefore its spread in the medical and non-medical areas should be limited by disinfection. Methods: Survival of C. albicans was estimated with using principles of PN-EN 16615 standard. Suspension of C. albicans with interfering substances (0,3 g/l bovine albumin) was dry on PCV carriers under a laminar chamber without fan, at the ambient temperature for no longer than 60 minutes. C. albicans was recovered from the carriers immediately after drying and after contact time (1; 5, 10, 20, 40; 60 minutes). Results: The drying conditions applied reduced the recovery of C. albicans from 1.21 to 2.07 on a logarithmic decimal scale with respect to the test suspension with interfering substances. The difference between the recovery immediately after drying and the recovery after the tested contact times (up to 60 minutes) was insignificant. Conclusions: Achieving the number of C. albicans after the drying process, as provided for in PN-EN 16615, requires further improvement of the drying conditions or an increase of C. albicans suspension density before drying.

Determination of Dimetridazole and Ipronidazole in Feeds at Cross-Contamination Levels

1988 ◽  
Vol 71 (3) ◽  
pp. 474-477 ◽  
Author(s):  
Duane D Hughes
Keyword(s):  
Thin Layer ◽  
Rapid Method ◽  
Aqueous Phase ◽  
Borate Buffer ◽  
Cross Contamination ◽  
Chromatographic System ◽  
Arsanilic Acid ◽  
Contamination Levels ◽  
Benzene Extract

Abstract A rapid method for the determination of dimetridazole and ipronidazole in feeds is described. The compounds are extracted from a borate buffer (pH 8.65) with benzene, partitioned into IN HC1, and then partitioned back into benzene from a basic aqueous phase. The benzene extract is concentrated and injected onto a nonpolar (Apiezon L) gas chromatographic column for determination by 63Ni electroncapture detection. Recoveries from feeds of various composition, spiked at 0.2 ppm with both dimetridazole and ipronidazole, ranged from 70 to 115%; for the same feeds spiked at 1 ppm or more, the recoveries were greater than 80%. Carbadox, furazolidone, levamisole, oxytetracycline, chlortetracycline, sulfamethazine, sulfaquinoxaline, arsanilic acid, piperazine, penicillin, and commonly added vitamins and minerals do not interfere. A 2-dimensional thin layer chromatographic system is presented as a means of additional identification.

Kinetic Study of Methyl Orange Adsorption from Solution by IOCZ

2013 ◽  
Vol 684 ◽  
pp. 194-197
Author(s):  
Yi Ke Li ◽  
Bing Lu Zhao ◽  
Wei Xiao ◽  
Run Ping Han ◽  
Yan Qiang Li
Keyword(s):  
Methyl Orange ◽  
Contact Time ◽  
Adsorption Mechanism ◽  
Order Model ◽  
Initial Concentration ◽  
Pseudo Second Order ◽  
Adsorption From Solution ◽  
Adsorption Quantity ◽  
Regressive Analysis

The effect of contact time and the determination of the kinetic parameters of adsorption of methyl orange (MO) from aqueous solution onto Iron-Oxide-Coated-Zeolite (IOCZ) powder are important in understanding the adsorption mechanism. The effect of contact time on adsorption quantity was studied at different initial concentration and temperature, respectively. The pseudo-second-order model was adopted to fit the experimental data using non-linear regressive analysis and it was used to predict the adsorption behavior. The results showed that the process of adsorption MO was endothermic and chemisorption. The pore diffusion was not significant.

STAPHYLOCOCCUS AUREUS SENSITIVITY TO ANTIBIOTICS IN CHILDREN WITH ATOPIC DERMATITIS

2017 ◽  
Vol 14 (6) ◽  
pp. 21-25
Author(s):  
E E Varlamov ◽  
E S Fedenko ◽  
M C Treneva ◽  
V R Voronina ◽  
Z V Zaporozhtseva ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Atopic Dermatitis ◽  
Antibiotic Resistance ◽  
Secondary Infection ◽  
Skin Scraping ◽  
Microbiological Examination ◽  
Antibiotics Sensitivity

Background. The aim of this study was to assess the antibiotic resistance of S. aureus isolated from the skin of atopic dermatitis (AD) children. Materials and methods. 256 AD children were included in the study in 2014-2016. Microbiological examination of skin scraping and determination of antibiotics sensitivity were performed. Results. Staphylococcus aureus was isolated in 173 (67,6%) of 256 patients. Resistance to erythromycin was established in 64,6 and 74,6%, respectively. Retrospective analyses showed that S. aureus became more sensitive to ampicillin, ceftriaxone, ciprofloxacin but more resistant to erythromycin. Conclusion. Ceftriaxone and cefoxitin but not erythromycin and ampicillin are the drugs of choice for the treatment of atopic dermatitis complicated by secondary infection in children.

Determination of the minimum suppressive concentration of photosensitizers of the second generation in relation to sanitaryindicative microorganisms

2021 ◽  
Vol 1 (11) ◽  
pp. 42-46
Author(s):  
Vadim Yu. Shlenskiy ◽  
◽  
Tatiana I. Pashnik ◽  
Svetlana Yu. Pigina ◽  
◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Salmonella Enteritidis ◽  
Second Generation ◽  
Research Work ◽  
Protoporphyrin Ix ◽  
Bactericidal Effect ◽  
Disodium Salt ◽  
Bacterial Cultures

In the studies described in the article, the presence of antimicrobial activity in photosensitizers in experiments on bacterial cultures was confirmed. In the experiments, photosensitizers of the second generation were used: «Photoditazine», the active substance of which is dimeglumin chloride E6, and «Dimegin», which includes a derivative of protoporphyrin IX-2,4-di(1-methoxyethyl)- deuteroporphyrin IX disodium salt. In the described research work, the minimum concentrations of the studied drugs were determined by the method of sequential tenfold dilutions to obtain a bactericidal effect on strains of sanitary-indicative microorganisms Enterococcus faecalis, Staphylococcus aureus, Salmonella enteritidis.

scholarly journals Determination of the maximum inhibitory dilution of cetylpyridinium chloride-based mouthwashes against staphylococcus aureus: an in vitro study

2008 ◽  
Vol 16 (4) ◽  
pp. 275-279 ◽  
Author(s):  
Evandro Watanabe ◽  
Juliane Maria Guerreiro Tanomaru ◽  
Andresa Piacezzi Nascimento ◽  
Fumio Matoba-Júnior ◽  
Mario Tanomaru-Filho ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cetylpyridinium Chloride ◽  
In Vitro Study ◽  
Vitro Study

Emergency medical services oxygen equipment: a fomite for transmission of MRSA?

2018 ◽  
pp. emermed-2018-207758
Author(s):  
Cody Vaughn Gibson
Keyword(s):  
Staphylococcus Aureus ◽  
Emergency Medical Services ◽  
Prehospital Setting ◽  
Medical Services ◽  
Cross Contamination ◽  
Nacl Solution ◽  
Emergency Medical ◽  
Storage Area ◽  
Oxygen Equipment ◽  
Patient Infection

ObjectivesThe primary purpose of this study was to determine if methicillin-resistant Staphylococcus aureus (MRSA) was present on the surface of oxygen cylinders and regulators used in the prehospital setting and secondarily to assess other surfaces for MRSA within the ambulance compartment, as a comparison.MethodsOn 17 March 2018, the surface of oxygen cylinders and regulators located in ambulances at an emergency medical services (EMS) station in North Alabama (n=9) and at an offsite oxygen cylinder storage area (n=70) were swabbed using sterile cotton-tipped applicators saturated in an 0.9% NaCl solution. These cotton-tipped applicators were then streaked across the surface of HardyCHROM MRSA plates, followed by incubation at 36°C for 24 hours. The growth of pink or magenta colonies was considered a positive indication for the presence of MRSA. The motivation for assessing oxygen cylinders at the offsite storage area was to outline the persistence of MRSA on stored oxygen cylinders.ResultsOf nine oxygen cylinders tested in the ambulances, nine had MRSA colonisation (100%). MRSA was also present on 67 of 70 oxygen cylinders (96%) tested at the offsite oxygen cylinder storage area.ConclusionOxygen cylinders appear to act as a fomite for MRSA. The development of universal disinfection protocols for oxygen equipment could help reduce the risk of patient infection due to cross-contamination.

Sign in / Sign up

Export Citation Format

Share Document