scholarly journals Cross-linking Immunoprecipitation High-throughput Sequencing

2020 ◽  
Author(s):  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Cross Linking

scholarly journals Argonaute High-Throughput Sequencing of RNAs Isolated by Cross-Linking Immunoprecipitation Reveals a Snapshot of miRNA Gene Regulation in the Mammalian Retina

Biochemistry ◽  
2014 ◽  
Vol 53 (37) ◽  
pp. 5831-5833 ◽  
Author(s):  
Thomas R. Sundermeier ◽  
Hui Jin ◽  
Matthew L. Kleinjan ◽  
Debarshi Mustafi ◽  
Donny D. Licatalosi ◽  
...  
Keyword(s):  
Gene Regulation ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Mirna Gene ◽  
Cross Linking ◽  
Mammalian Retina

High-throughput sequencing methods to study neuronal RNA–protein interactions

2009 ◽  
Vol 37 (6) ◽  
pp. 1278-1280 ◽  
Author(s):  
Jernej Ule
Keyword(s):  
Transcriptional Regulation ◽  
High Throughput ◽  
Protein Interactions ◽  
Translational Control ◽  
High Throughput Sequencing ◽  
Ribosome Profiling ◽  
Cross Linking ◽  
Rnase Protection ◽  
Temporal Precision ◽  
Post Transcriptional Regulation

UV-cross-linking and RNase protection, combined with high-throughput sequencing, have provided global maps of RNA sites bound by individual proteins or ribosomes. Using a stringent purification protocol, UV-CLIP (UV-cross-linking and immunoprecipitation) was able to identify intronic and exonic sites bound by splicing regulators in mouse brain tissue. Ribosome profiling has been used to quantify ribosome density on budding yeast mRNAs under different environmental conditions. Post-transcriptional regulation in neurons requires high spatial and temporal precision, as is evident from the role of localized translational control in synaptic plasticity. It remains to be seen if the high-throughput methods can be applied quantitatively to study the dynamics of RNP (ribonucleoprotein) remodelling in specific neuronal populations during the neurodegenerative process. It is certain, however, that applications of new biochemical techniques followed by high-throughput sequencing will continue to provide important insights into the mechanisms of neuronal post-transcriptional regulation.

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