scholarly journals GDNF Gene

2020 ◽  
Author(s):  
Keyword(s):  
Gdnf Gene

Association analysis of the glial cell line-derived neurotrophic factor (GDNF) gene in schizophrenia

2007 ◽  
Vol 97 (1-3) ◽  
pp. 271-276 ◽  
Author(s):  
H.J. Williams ◽  
N. Norton ◽  
T. Peirce ◽  
S. Dwyer ◽  
N.M. Williams ◽  
...  
Keyword(s):  
Cell Line ◽  
Glial Cell ◽  
Association Analysis ◽  
Neurotrophic Factor ◽  
Gdnf Gene

GDNF gene delivery via the p75NTR receptor rescues injured motor neurons

2006 ◽  
Vol 202 (1) ◽  
pp. 179-188 ◽  
Author(s):  
S BARATI ◽  
P HURTADO ◽  
S ZHANG ◽  
R TINSLEY ◽  
I FERGUSON ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Motor Neurons ◽  
Gdnf Gene

scholarly journals Adenovirus-Mediated Gene Transfer of Glial Cell Line-Derived Neurotrophic Factor Prevents Ischemic Brain Injury after Transient Middle Cerebral Artery Occlusion in Rats

1999 ◽  
Vol 19 (12) ◽  
pp. 1336-1344 ◽  
Author(s):  
Hisashi Kitagawa ◽  
Chihoko Sasaki ◽  
Kenichi Sakai ◽  
Atsushi Mori ◽  
Yasuhide Mitsumoto ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Brain Injury ◽  
Gene Transfer ◽  
Neurotrophic Factor ◽  
Treated Group ◽  
Artery Occlusion ◽  
Ischemic Brain Injury ◽  
Ischemic Brain ◽  
Gdnf Gene ◽  
Cerebral Artery Occlusion

To examine a possible protective effect of exogenous glial cell line-derived neurotrophic factor (GDNF) gene expression against ischemic brain injury, a replication-defective adenoviral vector containing GDNF gene (Ad-GDNF) was directly injected into the cerebral cortex at 1 day before 90 minutes of transient middle cerebral artery occlusion (MCAO) in rats. 2,3,5-Triphenyltetrazolium chloride staining showed that infarct volume of the Ad-GDNF-injected group at 24 hours after the transient MCAO was significantly smaller than that of vehicle- or Ad-LacZ-treated group. Enzyme-linked immunosorbent assay (ELISA) for immunoreactive GDNF demonstrated that GDNF gene products in the Ad-GDNF-injected group were higher than those of vehicle-treated group at 24 hours after transient MCAO. Immunoreactive GDNF staining was obviously detected in the cortex around the needle track just before or 24 hours after MCAO in the Ad-GDNF group, whereas no or slight GDNF staining was detected in the vehicle group. The numbers of TUNEL, immunoreactive caspase-3, and cytochrome c-positive neurons induced in the ipsilateral cerebral cortex at 24 hours after transient MCAO were markedly reduced by the Ad-GDNF group. These results suggest that the successful exogenous GDNF gene transfer ameliorates ischemic brain injury after transient MCAO in association with the reduction of apoptotic signals.

Dopaminergic Neurons Protected from Degeneration by GDNF Gene Therapy

Science ◽  
1997 ◽  
Vol 275 (5301) ◽  
pp. 838-841 ◽  
Author(s):  
D. L. Choi-Lundberg
Keyword(s):  
Gene Therapy ◽  
Dopaminergic Neurons ◽  
Gdnf Gene

scholarly journals Feline Neural Progenitor Cells II: Use of Novel Plasmid Vector and Hybrid Promoter to Drive Expression of Glial Cell Line-Derived Neurotrophic Factor Transgene

2012 ◽  
Vol 2012 ◽  
pp. 1-9
Author(s):  
X. Joann You ◽  
Jing Yang ◽  
Ping Gu ◽  
Chee Gee Liew ◽  
Henry J. Klassen
Keyword(s):  
Progenitor Cells ◽  
Transgene Expression ◽  
Neural Progenitor Cells ◽  
Nuclear Genome ◽  
Plasmid Vector ◽  
Neural Progenitor ◽  
Cell Transplant ◽  
Gdnf Gene ◽  
Potential Risks ◽  
Virus Mutant

Sustained transgene expression is required for the success of cell transplant-based gene therapy. Most widely used are lentiviral-based vectors which integrate into the host genome and thereby maintain sustained transgene expression. This requires integration into the nuclear genome, and potential risks include activation of oncogenes and inactivation of tumor suppressor genes. Plasmids have been used; however lack of sustained expression presents an additional challenge. Here we used the pCAG-PyF101-eGFP plasmid to deliver the human GDNF gene to cat neural progenitor cells (cNPCs). This vector consists of a CAGG composite promoter linked to the polyoma virus mutant enhancer PyF101. Expression of an episomal eGFP reporter and GDNF transgene were stably maintained by the cells, even following induction of differentiation. These genetically modified cells appear suitable for use in allogeneic models of cell-based delivery of GDNF in the cat and may find veterinary applications should such strategies prove clinically beneficial.

Sign in / Sign up

Export Citation Format

Share Document