scholarly journals Laboratory Specimen Type

2020 ◽  
Author(s):  
Keyword(s):  
Specimen Type ◽  
Laboratory Specimen

scholarly journals A flexible, pan-species, multi-antigen platform for the detection and monitoring of SARS-CoV-2-specific antibody responses

2021 ◽  
Author(s):  
Huifeng Shen ◽  
David Forgacs ◽  
Digantkumar Chapla ◽  
Kelley W. Moremen ◽  
Lance Wells ◽  
...  
Keyword(s):  
Host Species ◽  
Unmet Need ◽  
Reference Points ◽  
Antibody Isotype ◽  
Neutralization Activity ◽  
Specific Antibodies ◽  
E Coli ◽  
Specimen Type ◽  
Antigen Assay ◽  
Antibody Levels

AbstractThe SARS-CoV-2 pandemic and the vaccination effort that is ongoing has created an unmet need for accessible, affordable, flexible and precise platforms for monitoring the induction, specificity and maintenance of virus-specific immune responses. Herein we validate a multiplex (Luminex-based) assay capable of detecting SARS-CoV-2-specific antibodies irrespective of host species, antibody isotype, and specimen type (e.g. plasma, serum, saliva or blood spots). The well-established precision of Luminex-based assays provides the ability to follow changes in antibody levels over time to many antigens, including multiple permutations of the most common SARS-CoV-2 antigens. This platform can easily measure antibodies known to correlate with neutralization activity as well as multiple non-SARS-CoV-2 antigens such as vaccines (e.g. Tetanus toxoid) or those from frequently encountered agents (influenza), which serve as stable reference points for quantifying the changing SARS-specific responses. All of the antigens utilized in our study can be made in-house, many in E. coli using readily available plasmids. Commercially sourced antigens may also be incorporated and newly available antigen variants can be rapidly produced and integrated, making the platform adaptable to the evolving viral strains in this pandemic.Brief SummaryA multi-antigen assay for monitoring SARS-CoV-2-specific antibodies irrespective of host species, antibody isotype, and specimen type was developed.

scholarly journals FATIGUE ASSESSMENT OF UNIVERSAL CARDAN JOINT BASED ON LABORATORY SPECIMEN TESTS

2017 ◽  
Author(s):  
Pietro Palma ◽  
Gianni Tiussi ◽  
Andrea Donadon ◽  
Yuri Raffaglio ◽  
Andrea De Luca ◽  
...  
Keyword(s):  
Fatigue Assessment ◽  
Cardan Joint ◽  
Laboratory Specimen

scholarly journals Deep throat saliva as an alternative diagnostic specimen type for the detection of SARS‐CoV‐2

2020 ◽  
Vol 93 (1) ◽  
pp. 533-536 ◽  
Author(s):  
Eddie Chi‐man Leung ◽  
Viola Chi‐ying Chow ◽  
May Kin‐ping Lee ◽  
Raymond Wai‐man Lai
Keyword(s):  
Specimen Type

Plasma vs. serum in circulating tumor DNA measurement: characterization by DNA fragment sizing and digital droplet polymerase chain reaction

2020 ◽  
Vol 58 (4) ◽  
pp. 527-532 ◽  
Author(s):  
Jee-Soo Lee ◽  
Miyoung Kim ◽  
Moon-Woo Seong ◽  
Han-Sung Kim ◽  
Young Kyung Lee ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Circulating Tumor Dna ◽  
Analytical Sensitivity ◽  
Dna Fragments ◽  
Chain Reaction ◽  
Specimen Type ◽  
Dna Fragment ◽  
Polymerase Chain ◽  
Ctdna Analysis ◽  
Tumor Dna

AbstractBackgroundChoosing the specimen type is the first step of the pre-analytical process. Previous reports suggested plasma as the optimal specimen for circulating tumor DNA (ctDNA) analysis. However, head-to-head comparisons between plasma and serum using platforms with high analytical sensitivity, such as droplet digital polymerase chain reaction (ddPCR), are limited, and several recent studies have supported the clinical utility of serum-derived ctDNA. This study aimed to compare the DNA profiles isolated from plasma and serum, characterize the effects of the differences between specimens on ctDNA measurement, and determine the major contributors to these differences.MethodsWe isolated cell-free DNA (cfDNA) from 119 matched plasma/serum samples from cancer patients and analyzed the cfDNA profiles by DNA fragment sizing. We then assessed KRAS mutations in ctDNA from matched plasma/serum using ddPCR.ResultsThe amount of large DNA fragments was increased in serum, whereas that of cfDNA fragments (<800 bp) was similar in both specimens. ctDNA was less frequently detected in serum, and the KRAS-mutated fraction in serum was significantly lower than that in plasma. The differences in ctDNA fractions between the two specimen types correlated well with the amount of large DNA fragments and white blood cell and neutrophil counts.ConclusionsOur results provided detailed insights into the differences between plasma and serum using DNA fragment sizing and ddPCR, potentially contributing to ctDNA analysis standardization. Our study also suggested that using plasma minimizes the dilution of tumor-derived DNA and optimizes the sensitivity of ctDNA analysis. So, plasma should be the preferred specimen type.

scholarly journals Shock-Sensitivity in Shell-Like Structures: With Simulations of Spherical Shell Buckling

2016 ◽  
Vol 26 (02) ◽  
pp. 1630003 ◽  
Author(s):  
J. Michael T. Thompson ◽  
Jan Sieber
Keyword(s):  
Spherical Shell ◽  
Severe Form ◽  
Nondestructive Technique ◽  
Nonlinear Load ◽  
Shock Sensitivity ◽  
Shell Buckling ◽  
General Terms ◽  
Lateral Disturbance ◽  
Laboratory Specimen ◽  
Asymmetric Solution

Under increasing compression, an unbuckled shell is in a metastable state which becomes increasingly precarious as the buckling load is approached. So to induce premature buckling, a lateral disturbance will have to overcome a decreasing energy barrier which reaches zero at buckling. Two archetypal problems that exhibit a severe form of this behavior are the axially-compressed cylindrical shell and the externally pressurized spherical shell. Focusing on the cylinder, a nondestructive technique was recently proposed to estimate the “shock-sensitivity” of a laboratory specimen using a lateral probe to measure the nonlinear load-deflection characteristic. If a symmetry-breaking bifurcation is encountered on the path, computer simulations showed how this can be suppressed by a controlled secondary probe. Here, we extend our understanding by assessing in general terms how a single control can capture remote saddle solutions: in particular, how a symmetric probe could locate an asymmetric solution. Then, more specifically, we analyze the spherical shell with point and ring probes, to test the procedure under challenging conditions to assess its range of applicability. Rather than a bifurcation, the spherical shell offers the challenge of a destabilizing fold (limit point) under the rigid control of the probe.

scholarly journals 2025. Procalcitonin Reference Cutoff and Specimen-Type Validation

2018 ◽  
Vol 5 (suppl_1) ◽  
pp. S590-S590
Author(s):  
Muhammad Ahmad ◽  
Annaliza Adaya ◽  
Dipika Patel ◽  
Robert Benirschke ◽  
Hong Lee
Keyword(s):  
Specimen Type
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