scholarly journals Drug Sample

2020 ◽  
Author(s):  
Keyword(s):  
Drug Sample

Substituent dependent sensing behavior of Schiff base chemosensors in detecting Zn2+and Al3+ ions: Drug sample analysis and living cell imaging

2019 ◽  
Vol 282 ◽  
pp. 347-358 ◽  
Author(s):  
Bidyut Kumar Kundu ◽  
Poulami Mandal ◽  
Bhaswati Ghosh Mukhopadhyay ◽  
Ritudhwaj Tiwari ◽  
Debasis Nayak ◽  
...  
Keyword(s):  
Schiff Base ◽  
Living Cell ◽  
Cell Imaging ◽  
Sample Analysis ◽  
Drug Sample ◽  
Living Cell Imaging

scholarly journals EVALUATION AND COMPARISON OF CYTOTOXIC EFFECT OF VILAZODONE HYDROCHLORIDE WITH 5-FLUOROURACIL IN HT-29 BOWEL CANCER CELL LINE

2019 ◽  
pp. 124-127
Author(s):  
LATHA PRIYA A ◽  
ANUSHA D ◽  
DARLING CHELLATHAI K ◽  
HEMALATHA A ◽  
JEGAN MOHAN Y
Keyword(s):  
Cell Line ◽  
Cytotoxic Effect ◽  
Depressive Disorders ◽  
Cancer Cell Line ◽  
Test Sample ◽  
Standard Drug ◽  
Drug Sample ◽  
Diphenyl Tetrazolium Bromide ◽  
Ht 29

Objectives: Vilazodone hydrochloride is a novel selective serotonin reuptake inhibitor (SSRI) used to treat major depressive disorders. There are only sparse data available to know about the SSRI’s and its association with colon cancer. This study aims to evaluate and compare the in vitro cytotoxic effect of vilazodone with 5-fluorouracil (5-FU) in HT-29 cell line. Methods: Cell viability was tested by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay (Mosmann, 1983). Test sample and standard drug in variable concentrations were added to the HT-29 cell lines for incubation over 24 h under ideal conditions. After washing the test and standard drug sample from the well with saline, MTT was added and incubated for 4 h. Dimethyl sulfoxide of 1 ml was added in all wells after incubation with MTT. The absorbance at 570 nm was measured with an ultraviolet - spectrophotometer. Results: The values were tabulated, and the graph was plotted to find the IC-50 value (inhibitory concentration at 50%) which was struck at 28.5 μg/ml and12. 8 μg/ml for vilazodone hydrochloride and 5-FU, respectively. Conclusion: The results show that vilazodone hydrochloride has good anticancer property comparable with 5-FU, which would probably play a role as a cytotoxic agent in tumor cells. The proposed mechanism of action could be by activation of caspase-3 enzyme, thereby increasing apoptosis and indicates its use in coexisting depression and colon carcinoma. Other mechanism includes suppression of oncogene p53, which can be confirmed by future studies.

A Comparative Study on Anti-Microbial Efficacies of Biologically Synthesized Nano Gold Using Bos Taurus Urine with Pharmaceutical Drug Sample

2021 ◽  
Author(s):  
C. A. Pawar ◽  
A. K. Sharma ◽  
N. R. Prasad ◽  
S. S. Suryawanshi ◽  
G. M. Nazeruddin ◽  
...  
Keyword(s):  
Comparative Study ◽  
Bos Taurus ◽  
Pharmaceutical Drug ◽  
Nano Gold ◽  
Drug Sample

Drug Sample Control Cabinet

1959 ◽  
Vol 16 (4) ◽  
pp. 171-171
Author(s):  
Samuel Kohan
Keyword(s):  
Control Cabinet ◽  
Drug Sample

scholarly journals A physician survey of the effect of drug sample availability on physicians’ behavior

2000 ◽  
Vol 15 (7) ◽  
pp. 478-483 ◽  
Author(s):  
Lisa D. Chew ◽  
Theresa S. O’Young ◽  
Thomas K. Hazlet ◽  
Katharine A. Radley ◽  
Charles Maynard ◽  
...  
Keyword(s):  
Physician Survey ◽  
Drug Sample

scholarly journals Response: Use of Drug Sample Medications

2003 ◽  
Vol 16 (1) ◽  
pp. 86-87
Author(s):  
S. Schafer ◽  
J. Zweifler ◽  
S. Hughes
Keyword(s):  
Drug Sample

Liquid Chromatographic Determination of Colchicine in Pharmaceuticals: Collaborative Study

1985 ◽  
Vol 68 (5) ◽  
pp. 1051-1055
Author(s):  
Richard D Thompson
Keyword(s):  
Collaborative Study ◽  
Chromatographic Determination ◽  
Pharmaceutical Dosage Forms ◽  
Coefficients Of Variation ◽  
Drug Sample ◽  
Buffer Mixture ◽  
Liquid Chromatographic Determination ◽  
Bulk Drug ◽  
Liquid Chromatographic

Abstract A liquid chromatographic (LC) method for the determination of colchicine in pharmaceutical dosage forms and the bulk drug was evaluated in an interlaboratory study which included 13 participating laboratories. The method involves extraction (or dissolution) of the active ingredient with methanol-water (1 + 1), followed by filtration of the extract and reverse phase LC using an octy lsilane bonded phase column and UV detection at 254 nm. The mobile phase consists of a methanolphosphate buffer mixture (pH = 5.5). Three commercial tablet formulations (0.5-0.6 mg colchicine/tablet), 1 synthetic injectable preparation (0.510 mg colchicine/mL), and 1 bulk drug sample were assayed in duplicate by the proposed method. The reproducibility and repeatability standard deviations based on nonpooled results for each sample ranged from 0.0062 mg/mL to 0.0147 mg/tablet and from 0.0037 mg/ mL to 0.0127 mg/tablet, respectively; the corresponding coefficients of variation ranged from 1.21 to 2.54% and from 0.73 to 2.19%, respectively. The mean recovery from the synthetic injectable formulation was 100.0%. The method has been adopted official first action

scholarly journals A stepwise protocol for drug permeation assessment that combines heat-separated porcine ear epidermis and vertical diffusion cells

2018 ◽  
Vol 72 (1) ◽  
pp. 47-53 ◽  
Author(s):  
Ivana Pantelic ◽  
Tanja Ilic ◽  
Bojan Markovic ◽  
Sanela Savic ◽  
Milica Lukic ◽  
...  
Keyword(s):  
Evaluation Method ◽  
In Vitro Test ◽  
Formulation Development ◽  
Vertical Diffusion ◽  
Synthetic Membranes ◽  
Drug Permeation ◽  
Diffusion Cells ◽  
Drug Sample ◽  
Model Drug

After decades long absence of an official consensus on the most appropriate evaluation method for in vitro skin performance of topical semisolid drugs, United States Pharmacopoeia (USP 39) finally suggested three types of testing equipment; however, all these provide data on drug release using inert synthetic membranes. Considering the need for a readily available membrane that would be more structurally similar to human skin, this paper provides a detailed protocol of a method for drug permeation assessment that uses heat-separated porcine ear epidermis and modified Franz diffusion cells. Phases that were shown to be critical for variability of the results are identified (e.g., membrane preparation), and process parameters optimized. Applicability of the method was tested on four cream samples loaded with aceclofenac as a model drug. Sample compositions were designed in such a way to provide ?large? variations (variation of the main stabilizer: natural-origin versus synthetic emulsifier) and relatively ?minor? variations (co-solvent variation: none/isopropanol/glycerol). The developed protocol is a straightforward and reliable in vitro test for the evaluation of rate and extent of drug delivery into/through the skin. Moreover, this protocol may be routinely applied even in averagely equipped laboratories during formulation development or preliminary bioequivalence assessment of generic topical semisolids.

scholarly journals Development and Validation of Novel RP-HPLC Method for the Simultaneous Determination of Remogliflozin and Vildagliptin in Bulk and in synthetic Mixture

2021 ◽  
pp. 338-349
Author(s):  
Drashti A. Mandale ◽  
Chainesh Shah ◽  
Rakesh Jatt
Keyword(s):  
Single Dose ◽  
Dose Regimen ◽  
High Performance ◽  
Sglt2 Inhibitor ◽  
Hplc Method ◽  
Synthetic Mixture ◽  
Chromatography Method ◽  
Drug Sample ◽  
Liquid Chromatography Method

Vildagliptin which is DPP-4 inhibitor and Remogliflozin which is SGLT2 inhibitor in single dose regimen lower blood glucose by separate, complementary mechanisms. Both are glucose dependent, accounting for the low risk of hypoglycaemia during treatment. There is no risk factors associated with this combination and moreover it is single dose regimen. The aim of the present study was to develop and validate a simple, rapid and reproducible gradient high performance reverse phase liquid chromatography method for the estimation of Remogliflozin and Vildagliptin in bulk drug sample and in synthetic mixture using Xterra® Waters C18 column (150 mm×4.6 mm, 5 µm) at 25°C with UV detection at 210 nm and for this gradient mode was used. The compounds were eluted gradiently at a flow rate of 1.0ml/min. The average retention times for Remogliflozin and Vildagliptin were 4.881 and 6.334 min, respectively. The calibration curves were linear (r2 =0.988) over the concentration range 10-200 µg/ml for Remogliflozin and 10-200 µg/ml for Vildagliptin. No spectral or chromatographic interferences from formulation excipients were found and hence it was successfully applied for the determination of Remogliflozin and Vildagliptin in bulk and in synthetic mixture. The accuracy of the proposed method was determined by recovery studies and found to be 98-101%. The proposed method was validated and results conformed to ICH parameters.

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