scholarly journals Fluorescent Spot Test

2020 ◽  
Author(s):  
Keyword(s):  
Spot Test ◽  
Fluorescent Spot

Mass screening for glucose-6-phosphate dehydrogenase deficiency: improved fluorescent spot test

1985 ◽  
Vol 152 (1-2) ◽  
pp. 135-142 ◽  
Author(s):  
E. Solem ◽  
C. Pirzer ◽  
M. Siege ◽  
F. Kollmann ◽  
O. Romero-Saravia ◽  
...  
Keyword(s):  
Mass Screening ◽  
Dehydrogenase Deficiency ◽  
Spot Test ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Fluorescent Spot

Fluorescent spot test for detecting carbohydrate-splitting enzymes in meconium.

1981 ◽  
Vol 27 (3) ◽  
pp. 388-390 ◽  
Author(s):  
M C Hsieh
Keyword(s):  
Cystic Fibrosis ◽  
Ultraviolet Light ◽  
Screening Test ◽  
Spot Test ◽  
Fluorescent Compound ◽  
Present Procedure ◽  
Bright Fluorescence ◽  
Albumin Content ◽  
Fluorescent Products ◽  
Fluorescent Spot

Abstract I describe a fluorescent spot test for detecting the acidic glycosidases in meconium. Meconium is mixed with various 4-methylumbelliferylglycosides at the appropriate pH. In the presence of carbohydrate-splitting enzymes, 4-methylumbelliferone, a strongly fluorescent compound, is released. The reaction mixture, when spotted on chromatography paper and viewed under ultraviolet light, exhibits a bright fluorescence for specimens containing acidic glycosidases. The technique is applicable to any test that involves production of fluorescent products from non-fluorescent reactants. The procedure was applied to meconium specimens selected because of increased albumin content known to be associated with cystic fibrosis in newborns. The presence of beta-D-fucosidase in meconium was correlated to increased albumin content. Detection of beta-D-fucosidase by the present procedure may be useful as a screening test for cystic fibrosis.

Screening test for alpha 1-antitrypsin in dried-blood specimens.

1982 ◽  
Vol 28 (4) ◽  
pp. 615-617 ◽  
Author(s):  
A P Orfanos ◽  
E W Naylor ◽  
R Guthrie
Keyword(s):  
Screening Test ◽  
Dried Blood Spots ◽  
Spot Test ◽  
Fluorescent Compound ◽  
Antitrypsin Deficiency ◽  
Alpha 1 Antitrypsin Deficiency ◽  
Alpha 1 Antitrypsin ◽  
Antitrypsin Activity ◽  
Blood Specimens ◽  
Fluorescent Spot

Abstract We describe a fluorescent spot test for detecting alpha 1-antitrypsin activity in dried-blood specimens. The eluate of a blood disc is mixed with carbobenzoxy-L-arginine-7-amino-4-methylcoumarin amide and trypsin at the appropriate pH. In the absence of alpha 1-antitrypsin, aminomethylcoumarin, a strongly fluorescent compound, is released. The reaction mixture, when spotted on chromatography paper and viewed under ultraviolet light, exhibits a bright fluorescence only in the case of specimens with alpha 1-antitrypsin deficiency. alpha 1-Antitrypsin activity so estimated correlated well with quantitative assays of dried-blood spots and serum. The procedure is simple and inexpensive, and has the potential for use as a screening test.

Fluorescent Spot Test Method for Specific Detection of β-Lactamases

1994 ◽  
Vol 219 (1) ◽  
pp. 53-60 ◽  
Author(s):  
K.C.S. Chen ◽  
L. Chen ◽  
J.Y. Lin
Keyword(s):  
Spot Test ◽  
Test Method ◽  
Specific Detection ◽  
Fluorescent Spot
Sign in / Sign up

Export Citation Format

Share Document