scholarly journals Creatine Kinase MB to Total Creatine Kinase Ratio Measurement

2020 ◽  
Author(s):  
Keyword(s):  
Creatine Kinase ◽  
Ratio Measurement ◽  
Creatine Kinase Mb ◽  
Total Creatine

scholarly journals Influence of Gender on Total Serum Creatine Kinase and Creatine Kinase-Mb Levels of Nigerian Hypertensives

2021 ◽  
Vol 6 (2) ◽  
pp. 1436-1439
Author(s):  
Henry Afamuefuna Efobi ◽  
Iya Eze Bassey
Keyword(s):  
Blood Pressure ◽  
Body Mass Index ◽  
Creatine Kinase ◽  
Body Mass ◽  
The Body ◽  
Total Serum ◽  
Creatine Kinase Mb ◽  
Normotensive Subjects ◽  
Total Creatine ◽  
Gender Specific

Introduction: Hypertension is a long-term medical condition which when not detected and managed properly and on time often results to complications leading to disabilities and mortality. Gender has been known to affect the interpretation of some variables necessitating the need for gender-specific ranges. Objectives:This study evaluated total creatine kinase (CK) and creatine kinase-MB (CK-MB) isoenzyme activities of hypertensive subjects in Calabar, Nigeria and to assess if gender has influence on the activities of these enzymes. Methodology:One hundred and two participants were consecutively enrolled in this case control study. Serum total serum CK and CK-MB activity were evaluated in fifty one hypertensive and 51 normotensive subjects. The total CK levels were assessed using a spectrophotometric method while immunoinhibition method was used to determine the activity of CK-MB. Data was analyzed using Student’s t-test and Pearson’s correlation. Statistical significance was set at p<0.05. Results:The total creatine kinase activities of the hypertensives did not differ significantly from those of the normotensive controls (p = 0.428) while the serum CK-MB activities of the hypertensive subjects were significantly higher than those of the normotensive controls (p=0.000). The body mass index of the hypertensives was significantly higher than those of the normotensive subjects (p=0.030). Gender had no effect on the blood pressure, body mass index and levels of CK and CK-MB (p>0.05). There was also no significant correlation (p>0.05) between blood pressure, body mass index and the levels of CK and CK-MB. Conclusion:CK-MB activities were significantly higher in hypertensive subjects compared to normotensive controls. There were no gender specific differences in the CK-MB levels of male and female hypertensives. This cardiac marker should be included in the routine assessment of hypertensives and gender-specific considerations may not be necessary in the interpretation of the data.

Development and clinical evaluation of a microcentrifugal analyzer method for determining creatine kinase MB isoenzyme.

1983 ◽  
Vol 29 (3) ◽  
pp. 533-538
Author(s):  
T H Massey ◽  
W C Butts
Keyword(s):  
Creatine Kinase ◽  
Kinase Activity ◽  
Creatine Kinase Activity ◽  
Serum Samples ◽  
Inhibitory Antibody ◽  
Creatine Kinase Mb ◽  
Electrophoresis Method ◽  
Total Creatine Kinase Activity ◽  
Coronary Care ◽  
Total Creatine

Abstract We have adapted to a microcentrifugal analyzer an immunoinhibition assay for measuring the activity of creatine kinase MB by using an inhibitory antibody for the M monomer. The method actually measures half the MB activity, but results are not multiplied by two because atypical isoenzymes of creatine kinase, including BB, IgG-BB, and the isoenzyme derived from mitochondria, are also detected, if they are present. Results correlated well with an electrophoresis method for 36 serum samples. Myocardial infarction was assessed in 175 patients admitted to our coronary-care unit, with respect to sensitivity (100%) and specificity (98%) when a decision point of 100 U/L (30 degrees C) was chosen for total creatine kinase activity (dithiothreitol-activated) and 6 U/L (30 degrees C) for the isoenzyme (by immunoinhibition). Atypical isoenzymes are easily recognized and confirmed by electrophoresis when the MB activity (by immunoinhibition) exceeds 6 U/L and 20% of the total creatine kinase activity.

Use of creatine kinase MB isoenzyme for diagnosing myocardial infarction when total creatine kinase activity is high.

1988 ◽  
Vol 34 (11) ◽  
pp. 2208-2210 ◽  
Author(s):  
W G Thompson ◽  
R G Mahr ◽  
W S Yohannan ◽  
M R Pincus
Keyword(s):  
Myocardial Infarction ◽  
Creatine Kinase ◽  
Kinase Activity ◽  
Creatine Kinase Activity ◽  
Urban Hospital ◽  
Population Total ◽  
Creatine Kinase Mb ◽  
Total Creatine Kinase Activity ◽  
Total Creatine ◽  
Indigent Population

Abstract The usefulness of measuring creatine kinase MB isoenzyme for diagnosing myocardial infarction when activities of total creatine kinase are very high is unclear. We conducted a retrospective study in an urban hospital that serves a largely indigent population. We concentrated on 146 patients whose creatine kinase activity was greater than 1000 U/L (upper limit of normal: 165 U/L for women and 225 U/L for men), with MB isoenzyme greater than 10 U/L and less than 5% of total creatine kinase. The positive predictive value of MB isoenzyme (isoimmune method) values greater than 10 U/L was between 11.6% and 56.8% when the value for total creatine kinase exceeded 1000 U/L. Using different values (MB greater than 4% of total creatine kinase) as positive for myocardial infarction would have resulted in far fewer false-positives, but 10 cases of myocardial infarction would have been missed. The most appropriate cutoff value for MB isoenzyme in this population (total creatine kinase greater than 1000 U/L) was found to be greater than 2% of total creatine kinase.

Effect of DC shock on serum levels of total creatine kinase, MB-creatine kinase mass and troponin T

Resuscitation ◽  
1998 ◽  
Vol 36 (3) ◽  
pp. 193-199 ◽  
Author(s):  
Neil R Grubb ◽  
David Cuthbert ◽  
Paul Cawood ◽  
Andrew D Flapan ◽  
Keith A.A Fox
Keyword(s):  
Creatine Kinase ◽  
Troponin T ◽  
Serum Levels ◽  
Creatine Kinase Mb ◽  
Total Creatine

Radioimmunoassay of Human Cardiac Tropomyosin in Acute Myocardial Infarction

1981 ◽  
Vol 60 (3) ◽  
pp. 251-259 ◽  
Author(s):  
P. Cummins ◽  
B. McGurk ◽  
W. A. Littler
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Creatine Kinase ◽  
Time Course ◽  
False Negative ◽  
Peak Level ◽  
Negative Results ◽  
Creatine Kinase Mb ◽  
Human Sera ◽  
Total Creatine

1. Tropomyosin was prepared from fresh human myocardium and antisera raised in rabbits. A sensitive radioimmunoassay was developed for the detection of human cardiac tropomyosin in human sera down to levels of 1 ng/ml. 2. Values for human cardiac tropomyosin in normal patients ranged from less than 1 to 3 ng/ml. In 18 patients with acute myocardial infarction all had elevated tropomyosin levels ranging from 41 to above 200 ng/ml with a mean peak level of 101 ng/ml. In this study there were no false positive or false negative results. 3. In the initial stages of infarction the time course of appearance and peak levels of cardiac tropomyosin, total creatine kinase and creatine kinase MB isoenzyme were similar. Although total creatine kinase and creatine kinase MB isoenzyme levels were normal after 72 h in patients with single, uncomplicated infarction, cardiac tropomyosin levels were still significantly elevated above normal after this time, being 30–60% of peak values. 4. Radioimmunoassay of human cardiac tropomyosin may prove useful in the diagnosis and in the management of patients with acute myocardial infarction, particularly in the long-term postinfarction period.

Secretion of creatine kinase MB isoenzyme by an immature teratoma with predominant rhabdomyosarcomatous elements.

1988 ◽  
Vol 34 (12) ◽  
pp. 2600-2602 ◽  
Author(s):  
K Emancipator ◽  
A P Kudelka ◽  
G Bradford ◽  
K A Leonard ◽  
M H Zarrabi
Keyword(s):  
Creatine Kinase ◽  
Coronary Atherosclerosis ◽  
Kinase Activity ◽  
Creatine Kinase Activity ◽  
Immature Teratoma ◽  
Malignant Teratoma ◽  
Creatine Kinase Mb ◽  
Total Creatine Kinase Activity ◽  
Total Creatine ◽  
Increased Activity

Abstract A 37-year-old man with metastatic immature (malignant) teratoma with prominent rhabdomyosarcomatous elements had markedly increased activity of creatine kinase (EC 2.7.3.2) MB in serum. There was no electrocardiographic evidence of infarction or ischemia, and autopsy revealed no myocardial infarction, significant coronary atherosclerosis, myocarditis, or invasion of the heart by tumor. A high proportion of the creatine kinase activity in a homogenate of the tumor was attributable to the MB isoenzyme. Persistent increases of creatine kinase-MB and an unusually high MB isoenzyme activity, out of proportion to total creatine kinase activity, may indicate a nonmyocardial origin of this isoenzyme.

Detection of Cardiac-Specific Creatine Kinase Isoenzyme in Sera with Normal or Slightly Increased Total Creatine Kinase Activity

1975 ◽  
Vol 21 (8) ◽  
pp. 1088-1092 ◽  
Author(s):  
Donald W Mercer ◽  
Murray A Varat
Keyword(s):  
Creatine Kinase ◽  
Time Course ◽  
Kinase Activity ◽  
Creatine Kinase Activity ◽  
Good Reproducibility ◽  
Kinetic Assay ◽  
Creatine Kinase Isoenzyme ◽  
Creatine Kinase Mb ◽  
Total Creatine Kinase Activity ◽  
Total Creatine

Abstract We describe a spectrophotometric kinetic assay for detecting creatine kinase MB isoenzyme activity in the 1 to 10 U/liter range. The MB isoenzyme was isolated [Clin. Chem. 20, 36 (1974)] and assayed (Rosalki method) with an Abbott ABA-100. Good reproducibility was demonstrated for MB isoenzyme activities near 1 U/liter (CV = 2.6%). Sera with normal or slightly increased total creatine kinase activity were evaluated. Sera of 14 patients with acute myocardial infarction contained, per liter, 84 to 236 U of total creatine kinase activity and 4.6 to 28.0 U of isoenzyme MB activity; corresponding ranges for sera from healthy lab technicians and patients with noncardiac disease were 36 to 277 and 0 to 2.6 U. MB isoenzyme activity for infarction patients rose and fell sharply within three days after the infarction. Atypical time-course patterns, MB isoenzyme activity remaining abnormally great for five days, were observed in serum from patients with prolonged atrial fibrillation and congestive heart failure or cardiomyopathy; the BB isoenzyme (1 to 5 U/liter) was also detected in sera of such patients but was absent in sera from infarction patients. Quantification of column-isolated MB by the assay described is rapid, easy, specific, and extremely sensitive for measuring MB in the 1 to 10 U/liter range.

Serum creatine kinase MM isoforms in hypertrophic cardiomyopathy

1991 ◽  
Vol 81 (6) ◽  
pp. 723-726 ◽  
Author(s):  
Mareomi Hamada ◽  
Takashi Ohtani ◽  
Michihito Sekiya ◽  
Yasushi Fujiwara ◽  
Takumi Sumimoto ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Hypertrophic Cardiomyopathy ◽  
Normal Control ◽  
Activity Ratio ◽  
Serum Creatine Kinase ◽  
Myocardial Tissue ◽  
Control Subjects ◽  
Creatine Kinase Mb ◽  
Total Creatine

1. To determine whether a persistent release of creatine kinase from the myocardium occurs in patients with hypertrophic cardiomyopathy, the activities of serum creatine kinase MM isoforms were measured in 22 patients with hypertrophic cardiomyopathy and in 14 normal control subjects. 2. Serum creatine kinase MB activity was significantly higher in patients with hypertrophic cardiomyopathy (7.8 ± 3.8 i.u./l) than in normal control subjects (0.4 ± 0.8 i.u./l; P < 0.01). 3. Serum MMa, MMb and MMc activities in patients with hypertrophic cardiomyopathy were 19.4 ± 4.1%, 26.7 ± 2.5% and 33.5 ± 7.0% of the total creatine kinase MM activity, respectively. These values for each isoform were significantly different from those in normal control subjects (11.3 ± 3.0%, 21.5 ± 4.4% and 40.7 ± 7.0%, respectively). The MMa/MMc activity ratio was significantly higher in patients with hypertrophic cardiomyopathy (0.61 ± 0.25) than in normal control subjects (0.30 ± 0.10; P < 0.01). 4. Our results indicate that a small amount of the myocardial tissue isoform of creatine kinase MM (MMa) is constantly released in many patients with hypertrophic cardiomyopathy.

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