scholarly journals Prototheca wickerhamii

2020 ◽  
Author(s):  
Keyword(s):  
Prototheca Wickerhamii

scholarly journals Prototheca wickerhamii breast implant infection after reconstructive surgery: A new level of complexity

2021 ◽  
Author(s):  
Joanne S.K. Teh ◽  
Amalie E. Wilke ◽  
Simon M. Overstall ◽  
Jasmine C. Teng ◽  
Ruth Chin ◽  
...  
Keyword(s):  
Reconstructive Surgery ◽  
Breast Implant ◽  
Implant Infection ◽  
Prototheca Wickerhamii

scholarly journals Outbreak of Prototheca wickerhamii algaemia and sepsis in a tertiary care chemotherapy oncology unit

2018 ◽  
Vol 74 (4) ◽  
pp. 358-364 ◽  
Author(s):  
I.D. Khan ◽  
A.K. Sahni ◽  
Sourav Sen ◽  
R.M. Gupta ◽  
Atoshi Basu
Keyword(s):  
Tertiary Care ◽  
Prototheca Wickerhamii ◽  
Oncology Unit

Visualization of sporopollenin-containing pathogenic green micro-alga Prototheca wickerhamii by fluorescent in situ hybridization (FISH)

2009 ◽  
Vol 55 (4) ◽  
pp. 465-472 ◽  
Author(s):  
Ryohei Ueno
Keyword(s):  
Cell Wall ◽  
In Situ Hybridization ◽  
Cell Walls ◽  
Fluorescent In Situ Hybridization ◽  
Rapid Identification ◽  
Oligonucleotide Probes ◽  
Logarithmic Growth Phase ◽  
Prototheca Wickerhamii ◽  
Algal Genus

Fluorescent in situ hybridization (FISH) using taxon-specific, rRNA-targeted oligonucleotide probes is one of the most powerful tools for the rapid identification of harmful microorganisms. However, eukaryotic algal cells do not always allow FISH probes to permeate over their cell walls. Members of the pathogenic micro-algal genus Prototheca are characterized by their distinctive cell-wall component, sporopollenin, an extremely tough biopolymer that resists acid and alkaline hydrolysis, enzyme attack, and acetolysis. To our knowledge, there has been no report of the successful permeation by the oligonucleotide probes over the cell walls of unicellular green micro-algae, which contain sporopollenin. The DNA probes passed through the cell wall of Prototheca wickerhamii after treating the algal cells with cetyltrimethylammonium bromide (CTAB). Most cells in the middle logarithmic growth phase culture fluoresced when hybridized with the rRNA-targeted universal probe for eukaryotes, though individual cells included in this culture differed in the level of cell-wall vulnerability to attack by the polysaccharide-degrading enzyme, thus reflecting the different stages of the life cycle. This is the first report regarding the visualization of sporopollenin-containing, green micro-algal cells by FISH.

scholarly journals Successful treatment of multifocal pedal Prototheca wickerhamii infection in a feline immunodeficiency virus-positive cat with multiple Bowenoid in situ carcinomas containing papillomaviral DNA sequences

2017 ◽  
Vol 3 (1) ◽  
pp. 205511691668859 ◽  
Author(s):  
Allan E Kessell ◽  
Derek McNair ◽  
John S Munday ◽  
Richard Savory ◽  
Catriona Halliday ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Feline Immunodeficiency Virus ◽  
Skin Lesions ◽  
En Bloc Resection ◽  
Bloc Resection ◽  
En Bloc ◽  
First Case ◽  
Immunodeficiency Virus ◽  
Prototheca Wickerhamii

Case summary A 16-year-old, castrated male, feline immunodeficiency virus (FIV)-positive, domestic shorthair cat developed multiple skin lesions. Most of these were Bowenoid carcinoma in situ and contained DNA sequences consistent with Felis catus papillomavirus type 2. Two additional lesions that developed in the skin and subcutaneous tissues between the digital and carpal pads on the left forelimb and right hindlimb were shown by cytology, histology and culture to be caused by Prototheca wickerhamii. These lesions failed to improve in response to systemic therapy treatment with itraconazole, but excision by sharp en bloc resection with follow-up oral itraconazole therapy proved curative for one lesion, although the other lesion recurred, necessitating a second surgery. Relevance and novel information This is only the second reported case of feline protothecosis from Australia and the first case that has been cultured and identified to the species level. Also of great interest was the presence of multiple papillomavirus-associated neoplastic lesions, which may have afforded a portal of entry for the algal pathogen and the cat’s positive FIV status; the latter might have impacted on both viral and algal pathogenesis by effects on immunocompetence.

Thermotherapy in calcaneal infection due to Prototheca wickerhamii

2019 ◽  
Vol 37 (1) ◽  
pp. 62
Author(s):  
Eleda Coletta ◽  
Fernando Moreno-Mateo ◽  
Juan Manuel Gutierrez-Carrera ◽  
Miguel Ángel Bratos-Pèc)rez
Keyword(s):  
Prototheca Wickerhamii

scholarly journals Multiple Metabolic Roles for the Nonphotosynthetic Plastid of the Green Alga Prototheca wickerhamii

2005 ◽  
Vol 4 (2) ◽  
pp. 253-261 ◽  
Author(s):  
Tudor Borza ◽  
Cristina E. Popescu ◽  
Robert W. Lee
Keyword(s):  
Green Alga ◽  
De Novo ◽  
Living Organism ◽  
Chlorophyll Synthesis ◽  
Metabolic Functions ◽  
De Novo Purine Biosynthesis ◽  
Isoprenoid Metabolism ◽  
Intracellular Parasites ◽  
Prototheca Wickerhamii ◽  
Mg Chelatase

ABSTRACT The presence of plastids in diverse eukaryotic lineages that have lost the capacity for photosynthesis is well documented. The metabolic functions of such organelles, however, are poorly understood except in the case of the apicoplast in the Apicomplexa, a group of intracellular parasites including Plasmodium falciparum, and the plastid of the green alga Helicosporidium sp., a parasite for which the only host-free stage identified in nature so far is represented by cysts. As a first step in the reconstruction of plastid functions in a nonphotosynthetic, predominantly free-living organism, we searched for expressed sequence tags (ESTs) that correspond to nucleus-encoded plastid-targeted polypeptides in the green alga Prototheca wickerhamii. From 3,856 ESTs, we found that 71 unique sequences (235 ESTs) correspond to different nucleus-encoded putatively plastid-targeted polypeptides. The identified proteins predict that carbohydrate, amino acid, lipid, tetrapyrrole, and isoprenoid metabolism as well as de novo purine biosynthesis and oxidoreductive processes take place in the plastid of P. wickerhamii. Mg-protoporphyrin accumulation and, therefore, plastid-to-nucleus signaling might also occur in this nonphotosynthetic organism, as we identified a transcript which encodes subunit I of Mg-chelatase, the enzyme which catalyzes the first committed step in chlorophyll synthesis. Our data indicate a far more complex metabolism in P. wickerhamii's plastid compared with the metabolic pathways predicted to be located in the apicoplast of P. falciparum and the plastid of Helicosporidium sp.

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