scholarly journals N-Myc Proto-Oncogene Protein

2020 ◽  
Author(s):  
Keyword(s):  
Oncogene Protein

Analysis of Oncogene Protein Structure Using Small World Network Concept

2020 ◽  
Vol 15 (7) ◽  
pp. 732-740
Author(s):  
Neetu Kumari ◽  
Anshul Verma
Keyword(s):  
Amino Acids ◽  
Protein Structure ◽  
Degree Distribution ◽  
Protein Structures ◽  
Small World ◽  
Extreme Condition ◽  
Centrality Measures ◽  
Small World Network ◽  
Network Concept ◽  
Oncogene Protein

Background: The basic building block of a body is protein which is a complex system whose structure plays a key role in activation, catalysis, messaging and disease states. Therefore, careful investigation of protein structure is necessary for the diagnosis of diseases and for the drug designing. Protein structures are described at their different levels of complexity: primary (chain), secondary (helical), tertiary (3D), and quaternary structure. Analyzing complex 3D structure of protein is a difficult task but it can be analyzed as a network of interconnection between its component, where amino acids are considered as nodes and interconnection between them are edges. Objective: Many literature works have proven that the small world network concept provides many new opportunities to investigate network of biological systems. The objective of this paper is analyzing the protein structure using small world concept. Methods: Protein is analyzed using small world network concept, specifically where extreme condition is having a degree distribution which follows power law. For the correct verification of the proposed approach, dataset of the Oncogene protein structure is analyzed using Python programming. Results: Protein structure is plotted as network of amino acids (Residue Interaction Graph (RIG)) using distance matrix of nodes with given threshold, then various centrality measures (i.e., degree distribution, Degree-Betweenness correlation, and Betweenness-Closeness correlation) are calculated for 1323 nodes and graphs are plotted. Conclusion: Ultimately, it is concluded that there exist hubs with higher centrality degree but less in number, and they are expected to be robust toward harmful effects of mutations with new functions.

scholarly journals Mathematical modelling of promoter occupancies in MYC-dependent gene regulation

2017 ◽  
Vol 3 (2) ◽  
pp. 54
Author(s):  
Uwe Benary ◽  
Elmar Wolf ◽  
Jana Wolf
Keyword(s):  
Dna Binding ◽  
Mathematical Modelling ◽  
Expression Patterns ◽  
Cell Types ◽  
Osteosarcoma Cell ◽  
Genome Wide ◽  
Human Osteosarcoma Cell ◽  
Cell Type Specific ◽  
Binding Behaviour ◽  
Oncogene Protein

The human MYC proto-oncogene protein (MYC) is a transcription factor that plays a major role in the regulation of cell proliferation. Deregulation of MYC expression is often found in cancer. In the last years, several hypotheses have been proposed to explain cell type specific MYC target gene expression patterns despite genome wide DNA binding of MYC. In a recent publication, a mathematical modelling approach in combination with experimental data demonstrated that differences in MYC-DNA-binding affinity are sufficient to explain distinct promoter occupancies and allow stratification of distinct MYC-regulated biological processes at different MYC concentrations. Here, we extend the analysis of the published mathematical model of DNA-binding behaviour of MYC to demonstrate that the insights gained in the investigation of the human osteosarcoma cell line U2OS can be generalized to other human cell types.

Role of phosphatidylinositide metabolism in ras-induced Xenopus oocyte maturation

1990 ◽  
Vol 10 (3) ◽  
pp. 923-929
Author(s):  
B T Pan ◽  
G M Cooper
Keyword(s):  
Xenopus Oocytes ◽  
Second Messengers ◽  
Phospholipid Metabolism ◽  
Meiotic Maturation ◽  
Ras Oncogene ◽  
Ras Protein ◽  
32P Incorporation ◽  
Kinetics Of ◽  
Oncogene Protein

Microinjection of Xenopus oocytes with ras protein (p21) was used to investigate the role of phospholipid metabolism in ras-induced meiotic maturation. Induction of meiosis by ras was compared with induction by progesterone, insulin, and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). Neomycin, which specifically binds to phosphatidylinositides and inhibits their metabolism, blocked meiotic maturation induced by ras or insulin but not by progesterone or TPA. In addition, p21 and TPA, but not insulin or progesterone, stimulated the incorporation of 32Pi into oocyte lipids. ras protein specifically stimulated 32P incorporation into phosphatidylinositides, whereas both ras and TPA stimulated 32P incorporation into phosphatidylcholine and phosphatidylethanolamine. The stimulatory effect of p21 on phosphatidylinositide metabolism correlated with the dose response and kinetics of ras-induced meiotic maturation. In addition, the ras oncogene protein was more potent than the proto-oncogene protein both in inducing meiotic maturation and in stimulating phosphatidylinositide metabolism. These results indicate that phosphatidylinositide turnover is required for ras-induced meiosis and suggest that phosphatidylinositide-derived second messengers mediate the biological activity of ras in Xenopus oocytes.

Correlation of long interspersed element-1 open reading frame 1 and c-Met proto-oncogene protein expression in ovarian cancer

2019 ◽  
Vol 41 (11) ◽  
pp. 1293-1299 ◽  
Author(s):  
Eun-Ji Ko ◽  
Young Lim Oh ◽  
Heung Yeol Kim ◽  
Wan Kyu Eo ◽  
Hongbae Kim ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Protein Expression ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Long Interspersed Element ◽  
Oncogene Protein ◽  
Open Reading Frame 1

Differential Expression of SKI Oncogene Protein in Hemangiomas

2009 ◽  
Vol 141 (2) ◽  
pp. 213-218 ◽  
Author(s):  
M. Teresa ◽  
Melin Tan ◽  
Mark Tarango ◽  
Lou Fink ◽  
Martin Mihm ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Differential Expression ◽  
Cellular Proliferation ◽  
Vascular Tissue ◽  
Senior Author ◽  
Tissue Array ◽  
Basic Science Study ◽  
Immunohistochemical Studies ◽  
Ski Protein ◽  
Oncogene Protein

OBJECTIVE: The pathogenesis for benign tumorigenesis in hemangiomas is unknown. Oncogene proteins may be influential in this process. SKI proteins have been previously described in various malignancies. We investigated the differential expression of the SKI (sarcoma viral oncogene) protein in hemangiomas. STUDY DESIGN: Prospective basic science study. SUBJECTS AND METHODS: Paraffin-embedded hemangioma tissues were obtained from the senior author from 2005 to 2006. We created the first vascular tissue array composed of 12 hemangioma specimens at various stages of growth and anatomic location. Two cores were taken from each sample. Controls were also included. Immunohistochemical studies were performed using SKI, CD31, and Ki67. RESULTS: All 12 hemangioma tissues overexpressed the SKI protein. The staining pattern was perinuclear within the endothelial cells. The intensity of staining was inversely proportional to the growth stage. The endothelial cells that were SKI-positive were involved in active cell division. CONCLUSION: SKI oncogene protein is differentially and specifically expressed in hemangioma tissues. SKI acts as a transcriptional co-repressor and inhibits the TGF-β pathway, thus leading to uncontrolled cellular proliferation and transformation. All vascular controls were negative for SKI staining. CLINICAL SIGNIFICANCE OF STUDY: The SKI oncogene protein is upregulated by hemangiomas and may play a role in hemangioma tumorigenesis.

scholarly journals Extragastrointestinal Stromal Tumor during Pregnacy

2012 ◽  
Vol 2012 ◽  
pp. 1-3 ◽  
Author(s):  
Ilay Gözükara ◽  
T. U. Kutlu Dilek ◽  
Hüseyin Durukan ◽  
Duygu Düsmez Apa ◽  
Suna Kabil Kucur ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Gastrointestinal Stromal Tumors ◽  
Stromal Tumor ◽  
Stromal Tumors ◽  
Cd 117 ◽  
Extragastrointestinal Stromal Tumor ◽  
Mesenchymal Neoplasms ◽  
Oncogene Protein

Extragastrointestinal stromal tumors (EGISTs) are mesenchymal neoplasms without connection to the gastrointestinal tract. Gastrointestinal stromal tumors (GISTs) and EGIST are similar according to their clinicopathologic and histomorphologic features. Both of them most often express immunoreactivity for CD-117, a c-kit proto-oncogene protein. The coexistence of GIST and pregnancy is very rare, with only two cases reported in the literature. In this paper, we presented the first EGIST case during pregnancy in the literature.

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