scholarly journals Imprinting Gene

2020 ◽  
Author(s):  
Keyword(s):  
Imprinting Gene

TRIM28 maintains genome imprints and regulates development of porcine SCNT embryos

Reproduction ◽  
2021 ◽  
Vol 161 (4) ◽  
pp. 411-424
Author(s):  
Yanhui Zhai ◽  
Meng Zhang ◽  
Xinglan An ◽  
Sheng Zhang ◽  
Xiangjie Kong ◽  
...  
Keyword(s):  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Transcript Abundance ◽  
Dna Demethylation ◽  
Epigenetic Variability ◽  
Promoter Regions ◽  
Cell Stage ◽  
Genome Wide ◽  
Developmental Progression ◽  
Imprinting Gene

Pre-implantation embryos undergo genome-wide DNA demethylation, however certain regions, like imprinted loci remain methylated. Further, the mechanisms ensuring demethylation resistance by TRIM28 in epigenetic reprogramming remain poorly understood. Here, TRIM28 was knocked down in oocytes, and its effects on porcine somatic cell nuclear transfer (SCNT) embryo development was examined. Our results showed that SCNT embryos constructed from TRIM28 knockdown oocytes had significantly lower cleavage (53.9 ± 3.4% vs 64.8 ± 2.7%) and blastocyst rates (12.1 ± 4.3% vs 19.8 ± 1.9%) than control-SCNT embryos. The DNA methylation levels at the promoter regions of the imprinting gene IGF2 and H19 were significantly decreased in the 4-cell stage, and the transcript abundance of other imprinting gene was substantially increased. We also identified an aberrant two-fold decrease in the expression of CXXC1and H3K4me3 methyltransferase (ASH2L and MLL2), and the signal intensity of H3K4me3 had a transient drop in SCNT 2-cell embryos. Our results indicated that maternal TRIM28 knockdown disrupted the genome imprints and caused epigenetic variability in H3K4me3 levels, which blocked the transcription activity of zygote genes and affected the normal developmental progression of porcine SCNT embryos.

Effects of the HDAC inhibitor scriptaid on the in vitro development of bovine embryos and on imprinting gene expression levels

2018 ◽  
Vol 110 ◽  
pp. 79-85 ◽  
Author(s):  
R. Laguna-Barraza ◽  
M.J. Sánchez-Calabuig ◽  
A. Gutiérrez-Adán ◽  
D. Rizos ◽  
S. Pérez-Cerezales
Keyword(s):  
Gene Expression ◽  
Hdac Inhibitor ◽  
Bovine Embryos ◽  
In Vitro Development ◽  
Expression Levels ◽  
Imprinting Gene ◽  
Vitro Development ◽  
Gene Expression Levels

EFFECTS OF ABERRANT EXPRESSION OF APOPTOSIS- RELATED AND IMPRINTING GENE ON FETAL DEVELOPMENT OF CLONED PIG FETUS

2007 ◽  
Vol 77 (Suppl_1) ◽  
pp. 124-124
Author(s):  
Hyun Ju Jung ◽  
Gi-Sun Im ◽  
Seongsoo Hwang ◽  
Hee-Ja Park ◽  
Na-Young Lee ◽  
...  
Keyword(s):  
Fetal Development ◽  
Aberrant Expression ◽  
Imprinting Gene ◽  
Cloned Pig

scholarly journals Urine mercury levels correlate with DNA methylation of imprinting gene H19 in the sperm of reproductive-aged men

PLoS ONE ◽  
2018 ◽  
Vol 13 (4) ◽  
pp. e0196314 ◽  
Author(s):  
Zhaoxu Lu ◽  
Yufeng Ma ◽  
Linying Gao ◽  
Yingjun Li ◽  
Qiang Li ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Imprinting Gene ◽  
Urine Mercury

scholarly journals Embryos aggregation improves development and imprinting gene expression in mouse parthenogenesis

2016 ◽  
Vol 58 (3) ◽  
pp. 270-279 ◽  
Author(s):  
Guang-Yu Bai ◽  
Si-Hang Song ◽  
Zhen-Dong Wang ◽  
Zhi-Yan Shan ◽  
Rui-Zhen Sun ◽  
...  
Keyword(s):  
Gene Expression ◽  
Imprinting Gene

Regulation of the H19 imprinting gene expression in human nasopharyngeal carcinoma by methylation

2003 ◽  
Vol 104 (2) ◽  
pp. 179-187 ◽  
Author(s):  
Aylwin Ng ◽  
Jing P. Tang ◽  
Christopher H.K. Goh ◽  
Kam M. Hui
Keyword(s):  
Gene Expression ◽  
Nasopharyngeal Carcinoma ◽  
Human Nasopharyngeal Carcinoma ◽  
Imprinting Gene
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