scholarly journals Feeder Layer

2020 ◽  
Author(s):  
Keyword(s):  
Feeder Layer

Elimination of feeder layer effects in cultured keratinocyte grafts

2000 ◽  
Vol 191 (4) ◽  
pp. S22 ◽  
Author(s):  
Wesley G Schooler ◽  
Kevin J Bruen ◽  
Suzan deSerres ◽  
Bruce A Cairns ◽  
Jeffrey A Frelinger ◽  
...  
Keyword(s):  
Feeder Layer

A Stro-1+ human universal stromal feeder layer to expand/maintain human bone marrow hematopoietic stem/progenitor cells in a serum-free culture system

2006 ◽  
Vol 34 (10) ◽  
pp. 1353-1359 ◽  
Author(s):  
Raquel Gonçalves ◽  
Cláudia Lobato da Silva ◽  
Joaquim M.S. Cabral ◽  
Esmail D. Zanjani ◽  
Graça Almeida-Porada
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Culture System ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Feeder Layer ◽  
Hematopoietic Stem ◽  
Serum Free

scholarly journals Design of the Artificial Acellular Feeder Layer for the Efficient Propagation of Mouse Embryonic Stem Cells

2008 ◽  
Vol 283 (39) ◽  
pp. 26468-26476 ◽  
Author(s):  
Masato Nagaoka ◽  
Yuko Hagiwara ◽  
Keiko Takemura ◽  
Yuta Murakami ◽  
Jixuan Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Feeder Layer

scholarly journals NANOG maintains self-renewal of primate ES cells in the absence of a feeder layer

2006 ◽  
Vol 11 (9) ◽  
pp. 1115-1123 ◽  
Author(s):  
Shin-ya Yasuda ◽  
Norihiro Tsuneyoshi ◽  
Tomoyuki Sumi ◽  
Kouichi Hasegawa ◽  
Takashi Tada ◽  
...  
Keyword(s):  
Es Cells ◽  
Feeder Layer ◽  
Self Renewal

Growth factor expression pattern of homologous feeder layer for culturing buffalo embryonic stem cell-like cells

2012 ◽  
Vol 24 (8) ◽  
pp. 1098 ◽  
Author(s):  
Ruchi Sharma ◽  
Aman George ◽  
Nitin M. Kamble ◽  
Manmohan S. Chauhan ◽  
Suresh Singla ◽  
...  
Keyword(s):  
Stem Cell ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Embryonic Stem ◽  
Transforming Growth Factor Β1 ◽  
Activin A ◽  
Feeder Layer ◽  
Es Cell ◽  
Fetal Fibroblasts ◽  
Tgf Β1

The present study examined the expression profile of buffalo fetal fibroblasts (BFF) used as a feeder layer for embryonic stem (ES) cell-like cells. The expression of important growth factors was detected in cells at different passages. Mitomycin-C inactivation increased relative expression levels of ACTIVIN-A, TGF-β1, BMP-4 and GREMLIN but not of fibroblast growth factor-2 (FGF-2). The expression level of ACTIVIN-A, transforming growth factor-β1 (TGF-β1), bone morphogenetic protein-4 (BMP-4) and FGF-2 was similar in buffalo fetal fibroblast (BFF) cultured in stem cell medium (SCM), SCM + 1000 IU mL–1 leukemia inhibitory factor (LIF), SCM + 5 ng mL–1 FGF-2 or SCM + LIF + FGF-2 for 24 h whereas GREMLIN expression was higher in FGF-2-supplemented groups. In spent medium, the concentration of ACTIVIN-A was higher in FGF-2-supplemented groups whereas that of TGF-β1 was similar in SCM and LIF + FGF-2, which was higher than when either LIF or FGF-2 was used alone. Following culture of ES cell-like cells on a feeder layer for 24 h, the TGF-β1 concentration was higher with LIF+FGF-2 than with LIF or FGF-2 alone which, in turn, was higher than that in SCM. In the LIF + FGF-2 group, the concentration of TGF-β1 was lower and that of ACTIVIN-A was higher in spent medium at 24 h than at 48 h of culture. These results suggest that BFF produce signalling molecules that may help in self-renewal of buffalo ES cell-like cells.

scholarly journals Irradiated Human Fibroblasts as a Substitute Feeder Layer to Irradiated Mouse 3T3 for the Culture of Human Corneal Epithelial Cells: Impact on the Stability of the Transcription Factors Sp1 and NFI

2019 ◽  
Vol 20 (24) ◽  
pp. 6296
Author(s):  
Gaëtan Le-Bel ◽  
Sergio Cortez Ghio ◽  
Louis-Philippe Guérin ◽  
Francis Bisson ◽  
Lucie Germain ◽  
...  
Keyword(s):  
Stem Cells ◽  
Transcription Factors ◽  
Cell Differentiation ◽  
Dna Binding ◽  
Epithelial Cells ◽  
Stem Cell Differentiation ◽  
Feeder Layer ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Human Corneal Epithelial Cells

Because of the worldwide shortage of graftable corneas, alternatives to restore visual impairments, such as the production of a functional human cornea by tissue engineering, have emerged. Self-renewal of the corneal epithelium through the maintenance of a sub-population of corneal stem cells is required to maintain the functionality of such a reconstructed cornea. We previously reported an association between stem cell differentiation and the level to which they express the transcription factors Sp1 and NFI. In this study, we investigated the impact of replacing irradiated 3T3 (i3T3) murine fibroblast feeder cells by irradiated human corneal fibroblasts (iHFL) on the expression of Sp1 and NFI and evaluated their contribution to the proliferative properties of human corneal epithelial cells (hCECs) in both monolayer cultures and human tissue engineered corneas (hTECs). hCECs co-cultured with iHFL could be maintained for up to two more passages than when they were grown with i3T3. Western Blot and electrophoretic mobility shift assays (EMSAs) revealed no significant difference in the feeder-layer dependent increase in Sp1 at both the protein and DNA binding level, respectively, between HCECs grown with either i3T3 or iHFL. On the other hand, a significant increase in the expression and DNA binding of NFI was observed at each subsequent passage when hCECs were co-cultured along with i3T3. These changes were found to result from an increased expression of the NFIA and NFIB isoforms in hCECs grown with i3T3. Exposure of hCECs to cycloheximide revealed an increased stability of NFIB that likely resulted from post-translational glycosylation of this protein when these cells were co-cultured with i3T3. In addition, iHFL were as efficient as i3T3 at preserving corneal, slow-cycling, epithelial stem cells in the basal epithelium of the reconstructed hTECs. Furthermore, we observed an increased expression of genes whose encoded products promote hCECs differentiation along several passages in hCECs co-cultured with either type of feeder layer. Therefore, the iHFL feeder layer appears to be the most effective at maintaining the proliferative properties of hCECs in culture most likely by preserving high levels of Sp1 and low levels of NFIB, which is known for its gene repressor and cell differentiation properties.

scholarly journals Activated macrophages as a feeder layer for growth of resident cardiac progenitor cells

2014 ◽  
Vol 68 (4) ◽  
pp. 665-674 ◽  
Author(s):  
Diana E. Sepúlveda ◽  
Patricia Cabeza Meckert ◽  
Paola Locatelli ◽  
Fernanda D. Olea ◽  
Néstor G. Pérez ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Cardiac Progenitor Cells ◽  
Feeder Layer ◽  
Activated Macrophages
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