scholarly journals RAG1 Gene

2020 ◽  
Author(s):  
Keyword(s):  
Rag1 Gene

scholarly journals N-terminal truncated human RAG1 proteins can direct T-cell receptor but not immunoglobulin gene rearrangements

Blood ◽  
2000 ◽  
Vol 96 (1) ◽  
pp. 203-209 ◽  
Author(s):  
Jeroen G. Noordzij ◽  
Nicole S. Verkaik ◽  
Nico G. Hartwig ◽  
Ronald de Groot ◽  
Dik C. van Gent ◽  
...  
Keyword(s):  
T Cell ◽  
Gene Mutation ◽  
T Cell Receptor ◽  
Severe Combined Immunodeficiency ◽  
Premature Stop Codon ◽  
Cell Receptor ◽  
Gene Rearrangements ◽  
Immunoglobulin Gene ◽  
Rag1 Gene ◽  
Immunoglobulin Gene Rearrangements

The proteins encoded by RAG1 and RAG2 can initiate gene recombination by site-specific cleavage of DNA in immunoglobulin and T-cell receptor (TCR) loci. We identified a new homozygous RAG1 gene mutation (631delT) that leads to a premature stop codon in the 5′ part of the RAG1 gene. The patient carrying this 631delT RAG1 gene mutation died at the age of 5 weeks from an Omenn syndrome-like T+/B−severe combined immunodeficiency disease. The high number of blood T-lymphocytes (55 × 106/mL) showed an almost polyclonal TCR gene rearrangement repertoire not of maternal origin. In contrast, B-lymphocytes and immunoglobulin gene rearrangements were hardly detectable. We showed that the 631delT RAG1 gene can give rise to an N-terminal truncated RAG1 protein, using an internal AUG codon as the translation start site. Consistent with the V(D)J recombination in T cells, this N-terminal truncated RAG1 protein was active in a plasmid V(D)J recombination assay. Apparently, the N-terminal truncated RAG1 protein can recombine TCR genes but not immunoglobulin genes. We conclude that the N-terminus of the RAG1 protein is specifically involved in immunoglobulin gene rearrangements.

scholarly journals Agronomic and Seed Traits of Soybean Lines with the Rag1 gene for Aphid Resistance

Crop Science ◽  
2010 ◽  
Vol 50 (5) ◽  
pp. 1891-1895 ◽  
Author(s):  
Justin L. Mardorf ◽  
Walter R. Fehr ◽  
Matthew E. O'Neal
Keyword(s):  
Aphid Resistance ◽  
Seed Traits ◽  
Rag1 Gene

Target-Selected Inactivation of the Zebrafish rag1 Gene

Science ◽  
2002 ◽  
Vol 297 (5578) ◽  
pp. 99-102 ◽  
Author(s):  
E. Wienholds
Keyword(s):  
Rag1 Gene

scholarly journals The Soybean Resistance Gene Rag1 Does Not Protect Against Soybean Cyst and Root-knot Nematodes

2009 ◽  
Vol 10 (1) ◽  
pp. 44
Author(s):  
Matthew Studham ◽  
Gustavo C. MacIntosh ◽  
Felicitas Avendaño ◽  
David Soh ◽  
Gregory L. Tylka
Keyword(s):  
Resistance Gene ◽  
Soybean Cyst Nematode ◽  
Soybean Aphid ◽  
Cyst Nematode ◽  
Root Knot Nematodes ◽  
Rag1 Gene ◽  
Tomato Gene ◽  
Soybean Resistance

The only well-characterized gene controlling soybean aphid (SBA) resistance is Mi-1.2, a tomato gene that also confers resistance to root-knot nematodes (RKN). Based on similarities between Mi-1.2 and Rag1, which produces a strong antibiosis-type resistance, the authors hypothesized that Rag1 could also provide resistance to nematodes. They evaluated two soybean lines, one carrying the Rag1 gene, and one with no resistance gene, for susceptibility or resistance to soybean cyst nematode (SCN) and RKN. Accepted for publication 4 March 2009. Published 1 April 2009.

The hexokinase gene is required for transcriptional regulation of the glucose transporter gene RAG1 in Kluyveromyces lactis

1993 ◽  
Vol 13 (7) ◽  
pp. 3882-3889
Author(s):  
C Prior ◽  
P Mamessier ◽  
H Fukuhara ◽  
X J Chen ◽  
M Wesolowski-Louvel
Keyword(s):  
Glucose Transporter ◽  
Kinase Activity ◽  
Glucose Repression ◽  
Kluyveromyces Lactis ◽  
Transport Systems ◽  
Transporter Gene ◽  
Rag1 Gene ◽  
Sugar Kinase

The RAG1 gene of Kluyveromyces lactis encodes a low-affinity glucose/fructose transporter. Its transcription is induced by glucose, fructose, and several other sugars. The RAG4, RAG5, and RAG8 genes are trans-acting genes controlling the expression of the RAG1 gene. We report here the characterization of one of these genes, RAG5. The nucleotide sequence of the cloned RAG5 gene indicated that it encodes a protein that is homologous to hexokinases of Saccharomyces cerevisiae. rag5 mutants showed no detectable hexokinase or glucokinase activity, suggesting that the sugar kinase activity encoded by this gene is the only hexokinase in K. lactis. Both high- and low-affinity transport systems of glucose were affected in rag5 mutants. The defect of the low-affinity component was found to be due to a block of transcription of the RAG1 gene by the hexokinase mutation. In vivo complementation of the rag5 mutation by the HXK2 gene of S. cerevisiae and complementation of hxk1 hxk2 mutations of S. cerevisiae by the RAG5 gene showed that RAG5 and HXK2 were equivalent for sugar-phosphorylating activity but that RAG5 could not restore glucose repression in the S. cerevisiae hexokinase mutants.

scholarly journals The hypoxic expression of the glucose transporter RAG1 reveals the role of the bHLH transcription factor Sck1 as a novel hypoxic modulator in Kluyveromyces lactis

2019 ◽  
Vol 19 (4) ◽  
Author(s):  
Rosa Santomartino ◽  
Daniela Ottaviano ◽  
Ilaria Camponeschi ◽  
Tracy Ann Alcarpio Landicho ◽  
Luca Falato ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Intracellular Signaling ◽  
Molecular Mechanisms ◽  
Glucose Transporter ◽  
Kluyveromyces Lactis ◽  
Transcriptional Activator ◽  
Bhlh Transcription Factor ◽  
Cellular Processes ◽  
Hypoxic Induction ◽  
Rag1 Gene

ABSTRACTGlucose is the preferred nutrient for most living cells and is also a signaling molecule that modulates several cellular processes. Glucose regulates the expression of glucose permease genes in yeasts through signaling pathways dependent on plasma membrane glucose sensors. In the yeast Kluyveromyces lactis, sufficient levels of glucose induction of the low-affinity glucose transporter RAG1 gene also depends on a functional glycolysis, suggesting additional intracellular signaling. We have found that the expression of RAG1 gene is also induced by hypoxia in the presence of glucose, indicating that glucose and oxygen signaling pathways are interconnected. In this study we investigated the molecular mechanisms underlying this crosstalk. By analyzing RAG1 expression in various K. lactis mutants, we found that the bHLH transcriptional activator Sck1 is required for the hypoxic induction of RAG1 gene. The RAG1 promoter region essential for its hypoxic induction was identified by promoter deletion experiments. Taken together, these results show that the RAG1 glucose permease gene is synergistically induced by hypoxia and glucose and highlighted a novel role for the transcriptional activator Sck1 as a key mediator in this mechanism.

Low-Affinity glucose carrier geneLGT1 ofSaccharomyces cerevisiae, a homologue of theKluyveromyces lactis RAG1 gene

Yeast ◽  
1993 ◽  
Vol 9 (12) ◽  
pp. 1373-1377 ◽  
Author(s):  
C. Prior ◽  
H. Fukuhara ◽  
J. Blaisonneau ◽  
M. Wesolowski-Louvel
Keyword(s):  
Rag1 Gene ◽  
Glucose Carrier
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