scholarly journals Microwell Plate

2020 ◽  
Author(s):  
Keyword(s):  
Microwell Plate

scholarly journals Colorimetric Microwell Plate Detection of the Factor V Leiden Mutation

2000 ◽  
Vol 46 (8) ◽  
pp. 1195-1198 ◽  
Author(s):  
Adalbert Kowalski ◽  
Daniela Radu ◽  
Bert Gold
Keyword(s):  
Factor V Leiden ◽  
Factor V ◽  
Factor V Leiden Mutation ◽  
Microwell Plate

Frequency domain fluorescence lifetime microwell-plate platform for respirometry measurements

2010 ◽  
Author(s):  
M. R. Chatni ◽  
G. Yale ◽  
A. Van Ryckeghem ◽  
D. M. Porterfield
Keyword(s):  
Frequency Domain ◽  
Fluorescence Lifetime ◽  
Microwell Plate

High-Throughput Microfabricated CE/ESI-MS:  Automated Sampling from a Microwell Plate

2001 ◽  
Vol 73 (11) ◽  
pp. 2675-2681 ◽  
Author(s):  
Bailin Zhang ◽  
Frantisek Foret ◽  
Barry L. Karger
Keyword(s):  
High Throughput ◽  
Esi Ms ◽  
Automated Sampling ◽  
Microwell Plate

scholarly journals Culture of Bovine Embryos on a Polydimethylsiloxane (PDMS) Microwell Plate

2010 ◽  
Vol 56 (4) ◽  
pp. 475-479 ◽  
Author(s):  
Satoshi AKAGI ◽  
Misa HOSOE ◽  
Kazutsugu MATSUKAWA ◽  
Akihiko ICHIKAWA ◽  
Tamio TANIKAWA ◽  
...  
Keyword(s):  
Bovine Embryos ◽  
Microwell Plate

Wax-Impregnated Cotton Fabrics as Cell Culture Platform

2015 ◽  
Vol 1112 ◽  
pp. 441-444 ◽  
Author(s):  
Norsamsiah Muhamad Wahab ◽  
Syazwani Abdul Jamil ◽  
Dwi Gustiono Riban ◽  
Fadzilah Adibah Abdul Majid ◽  
Mohammed Rafiq Abdul Kadir ◽  
...  
Keyword(s):  
Cell Culture ◽  
Soft Lithography ◽  
Cotton Fabrics ◽  
Base Layer ◽  
Human Skin Fibroblast ◽  
Proof Of Concept ◽  
Drying Process ◽  
Flat Bottom ◽  
Microwell Plate ◽  
Potential Use

Microwell plates are widely used in various cell-based assay and drug screening. Usually these plates are made from non-biodegradable materials such as polystyrene or polyethylene. In this report, we propose the use of wax-impregnated cotton fabrics as an alternative microwell plate that is easy to fabricate, simple to use, sustainable and environmentally friendlier. Several researchers recently used soft lithography and photolithography technique to fabricate various cell culture platforms. Our proposed method consists instead of simple dipping and drying process. The platform is made of a series of fabrics being stacked and held together with various wax formulations. With the exception of the base layer, each fabric layer has a circular hole opening with increasing diameter towards the top layer; thereby forming a well where cells are cultured at its flat bottom. We characterized the chemical and physical properties of the platform surface which affect cells attachment and proliferation. These properties include the surface chemistry, hydrophobicity and roughness. We cultured human skin fibroblast (HSF 1184 Cell Line) on the platform as our preliminary proof of concept. We observed proliferation of the cells after 24 hours. The result indicates the potential use of the platform for future cell-based assay applications.

scholarly journals A PCR-Colorimetric Microwell Plate Hybridization Assay for Detection of Mycobacterium tuberculosis and M. avium from Culture Samples and Ziehl-Neelsen-Positive Smears

2000 ◽  
Vol 38 (5) ◽  
pp. 1772-1776 ◽  
Author(s):  
Maria Cristina Rossi ◽  
Andrea Gori ◽  
Gianguglielmo Zehender ◽  
Giulia Marchetti ◽  
Giulio Ferrario ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Lymph Node Biopsy ◽  
Pericardial Fluid ◽  
Hybridization Assay ◽  
Rrna Gene ◽  
Mycobacterial Infections ◽  
Detection Assay ◽  
Alternative Approach ◽  
Target Dna ◽  
Microwell Plate

Differentiation between Mycobacterium tuberculosis andM. avium is essential for the treatment of mycobacterial infections. We have developed an easy and rapid detection assay for the diagnosis of mycobacterial diseases. This is a PCR-hybridization assay based on selective amplification of a 16S rRNA gene sequence using pan-Mycobacteriumprimers followed by hybridization of the amplification products to biotinylated M. tuberculosis and M. avium-specific probes. A total of 55 mycobacterial isolates were tested. For all isolates, results concordant with those of conventional identification methods were obtained. Moreover, we developed a method for extraction of DNA from Ziehl-Neelsen-positive smears which allows the recovery of intact target DNA in our PCR-hybridization assay. Our method was able to confirm all culture results for 59 Ziehl-Neelsen-positive smears from clinical specimens (35 sputum, 11 lymph node biopsy, 6 stool, 4 pus, 2 urine, and 1 pericardial fluid specimens). These data suggest that our PCR-hybridization assay, which is simple to perform and less expensive than commercial probe methods, may be suitable for the identification of M. tuberculosis and M. avium. It could become a valuable alternative approach for the diagnosis of mycobacterial infections when applied directly to DNA extracted from Ziehl-Neelsen-positive smears as well.

scholarly journals High-Throughput Optimization of Effective Microbes Nutrient Formulation via Automated Media Optimization System (AMOS)

2019 ◽  
Vol 7 (4.14) ◽  
pp. 1
Author(s):  
A I Saharuddin ◽  
N Z M N Rashid ◽  
N S Mohd Noor ◽  
N S Abdul Rahman ◽  
A Ismail ◽  
...  
Keyword(s):  
Growth Rate ◽  
Yeast Extract ◽  
Photosynthetic Bacteria ◽  
Animal Feed ◽  
Biomass Productivity ◽  
Dry Weight ◽  
Throughput Optimization ◽  
Beef Extract ◽  
Microwell Plate ◽  
Mrs Medium

Fortification of effective microbes (EM) such as Pediococcus acidilactici and photosynthetic bacteria in fertilizer and animal feed exhibiting effective outcomes in enhancing crops and livestock production as well as upholds sustainable agriculture concept. However, the productivity of the EM biomass stocks is unsurpassed to the demand due to non-optimized media. In this study, two sets of nutrient pair comprising of beef extract-manganese and yeast extract-glucose for P. acidilactici and photosynthetic bacteria respectively were studied. The optimum media formulated was based on the highest growth rate in 96-microwell plate system were subsequently tested in 1 L cultures volume for EM biomass production. Results showed that growth rate and dry weight of P. acidilactici in media supplemented with 64 g/L beef extract (0.145 h-1, 0.243 g) and 0.28 g/L manganese (0.1134 h-1, 0.32 g) were higher compared to control medium with an increment of 17.39 % and 54.59 % biomass productivity as compared to MRS medium respectively. Meanwhile, the growth rate and dry weight for Photosynthetic bacteria in media supplemented with 6.63 g/L glucose (0.2538 h-1, 0.167 g) and 0.44 g/L yeast extract (0.2733 h-1, 0.163 g) recorded 11.3 % and 8.73 % biomass productivity increment in comparison to the HCH medium.  

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