Retinoblastoma-Associated Protein Staining Method

Chalcones1~8and 5-deoxyflavonoids9~22were synthesized in good yields by aldol condensation, Algar-Flynn-Oyamada reaction, glycosidation, and deacetylation reaction, respectively, starting from 2-acetyl phenols substituted by methoxy or methoxymethoxy group and appropriately benzaldehydes substituted by methoxy, methoxymethoxy group, or chlorine. Among them,13and17~22are new compounds. The cytotoxicity bioassays of these chalcones and 5-deoxyflavonoids were screened using the sulforhodamine B (SRB) protein staining method, and the results showed that compounds2, 4, 5, 6, 10, 15, and19exhibited moderate cytotoxicity against the cancer cell line of MDA-MB-231, U251, BGC-823, and B16 in comparison with control drugs (HCPT, Vincristine, and Taxol).

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Genetic polymorphisms of the Pmol and Pmo2 salivary proteins detected by the modified protein staining method

Annals of Human Genetics ◽

10.1111/j.1469-1809.1988.tb01095.x ◽

1988 ◽

Vol 52 (3) ◽

pp. 177-187 ◽

Cited By ~ 5

Author(s):

K. MINAGUCHI ◽

K. SUZUKI

Keyword(s):

Genetic Polymorphisms ◽

Staining Method ◽

Salivary Proteins ◽

Protein Staining

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Ultrastructure and Staining of Developing Elastic Tissue

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100065274 ◽

1971 ◽

Vol 29 ◽

pp. 244-245

Author(s):

E. N. Albert

Keyword(s):

Fine Structure ◽

Phosphotungstic Acid ◽

Staining Method ◽

Rat Aorta ◽

Uranyl Acetate ◽

The Other ◽

Elastic Fibers ◽

Elastic Tissue ◽

Lead Citrate ◽

New Born

Silver tetraphenylporphine sulfonate (Ag-TPPS) was synthesized in this laboratory and used as an electron dense stain for elastic tissue (Fig 1). The procedures for the synthesis of tetraphenylporphine sulfonate and the staining method for mature elastic tissue have been described previously.The fine structure of developing elastic tissue was observed in fetal and new born rat aorta using tetraphenylporphine sulfonate, phosphotungstic acid, uranyl acetate and lead citrate. The newly forming elastica consisted of two morphologically distinct components. These were a central amorphous and a peripheral fibrous. The ratio of the central amorphous and the peripheral fibrillar portion changed in favor of the former with increasing age.It was also observed that the staining properties of the two components were entirely different. The peripheral fibrous component stained with uranyl acetate and/or lead citrate while the central amorphous portion demonstrated no affinity for these stains. On the other hand, the central amorphous portion of developing elastic fibers stained vigorously with silver tetraphenylporphine sulfonate, while the fibrillar part did not (compare figs 2, 3, 4). Based upon the above observations it is proposed that developing elastica consists of two components that are morphologically and chemically different.

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A pre-embedding, protein a-gold immunolabelling technique for cytocentrifuged cell monolayers for lm and tem

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100142724 ◽

1986 ◽

Vol 44 ◽

pp. 220-221

Author(s):

K. Chien ◽

I.P. Shintaku ◽

A.F. Sassoon ◽

R.L. Van de Velde ◽

R. Heusser

Keyword(s):

Cell Surface ◽

Staining Method ◽

Protein A ◽

Surface Antigens ◽

Cell Suspensions ◽

Cellular Morphology ◽

Cellular Phenotype ◽

Cell Surface Antigens ◽

Cell Monolayers ◽

Diagnostic Histopathology

Identification of cellular phenotype by cell surface antigens in conjunction with ultrastructural analysis of cellular morphology can be a useful tool in the study of biologic processes as well as in diagnostic histopathology. In this abstract, we describe a simple pre-embedding, protein A-gold staining method which is designed for cell suspensions combining the handling convenience of slide-mounted cell monolayers and the ability to evaluate specimen staining specificity prior to EM embedding.

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Enzyme histochemical application and backscattered electron imaging to visualize the distribution of lymphatic capillaries

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100161965 ◽

1990 ◽

Vol 48 (3) ◽

pp. 880-881

Author(s):

Seiji Kato

Keyword(s):

Staining Method ◽

Double Staining ◽

Reaction Products ◽

Blood Capillaries ◽

Backscattered Electron Imaging ◽

Histochemical Observation ◽

Backscattered Electron ◽

Double Staining Method ◽

Electron Imaging ◽

Cryostat Sections

Previously, the author repeatedly confirmed the higher 5’-nucleotidase (5’-Nase) and lower alkaline phoaphatase (ALPase) activities in the wall of lymphatic capillaries reacted with the lead-based method relative to those of blood capillaries. The ALPase, on the other hand, is markedly higher in blood capillaries than in lymphatics. On the basis of these enzyme characteristics, the author has developed a 5’-Nase— ALPase double staining method to differentiate small lymphatics from blood capillaries at the level of the light microcsopy. Furthermore, we applied it to histochemical observation of the lead-containing reaction products of 5’-Nase in lymphatics on the same or adjacent cryostat sections using backscattered electron imaging (BEI) in scanning electron microscope (SEM). This paper presents a new applicability of 5’-Nase histochemistry by BEI-SEM to demonstrate the distribution of lymphatic capillaries in tissue blocks.

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