scholarly journals HSF1 Gene

2020 ◽  
Author(s):  
Keyword(s):  
Hsf1 Gene

scholarly journals AKT1 mediates multiple phosphorylation events that functionally promote HSF1 activation

2020 ◽  
Author(s):  
Wen-Cheng Lu ◽  
Ramsey Omari ◽  
Haimanti Ray ◽  
Richard L. Carpenter
Keyword(s):  
Heat Shock ◽  
Functional Role ◽  
Transcriptional Activity ◽  
Heat Shock Factor 1 ◽  
Heat Stress Response ◽  
Subsequent Investigation ◽  
Important Regulator ◽  
Shock Proteins ◽  
Hsf1 Gene

AbstractThe heat stress response activates the transcription factor heat shock factor 1 (HSF1), which subsequently upregulates heat shock proteins to maintain the integrity of the proteome. HSF1 activity requires nuclear localization, trimerization, DNA binding, phosphorylation, and gene transactivation. Phosphorylation at S326 is an important regulator of HSF1 transcriptional activity. Phosphorylation at S326 is mediated by AKT1, mTOR, p38, and MEK1. mTOR, p38, and MEK1 all phosphorylated S326 but AKT1 was the more potent activator. Mass spectrometry showed that AKT1 phosphorylated HSF1 at T142, S230, and T527 in addition to S326 whereas the other kinases did not. Subsequent investigation revealed that phosphorylation at T142 is necessary for HSF1 trimerization and that S230, S326, and T527 are required for HSF1 gene transactivation and recruitment of TFIIB and CDK9. This study suggests that HSF1 activity is regulated by phosphorylation at specific residues that promote different stages of HSF1 activation. Furthermore, this is the first study to identify the functional role of these phosphorylation events.

scholarly journals Genetic identification of the site of DNA contact in the yeast heat shock transcription factor.

1995 ◽  
Vol 15 (9) ◽  
pp. 5063-5070 ◽  
Author(s):  
F A Torres ◽  
J J Bonner
Keyword(s):  
Transcription Factor ◽  
Heat Shock ◽  
Kluyveromyces Lactis ◽  
Heat Shock Transcription Factor ◽  
Genetic Identification ◽  
Complete Agreement ◽  
Magnetic Resonance Data ◽  
C Terminus ◽  
Mutant Sequence ◽  
Hsf1 Gene

The heat shock transcription factor (HSF), a trimeric transcription factor, activates the expression of heat shock genes in eukaryotes. We have isolated mutations in the HSF1 gene from Saccharomyces cerevisiae that severely compromise the ability of HSF to bind to its normal binding site, repeats of the module nGAAn. One of these mutations, Q229R, shows a "new specificity" phenotype, in which the protein prefers the mutant sequence nGACn. These results identify the region of HSF that contacts DNA, in complete agreement with the crystal structure of HSF of Kluyveromyces lactis and the nuclear magnetic resonance data from HSF of Drosophila melanogaster. To determine the orientation of the DNA-binding domain on the nGAAn motif, we performed site-specific cross-linking between cysteine residues of single-cysteine substitutions. Cysteines placed at the N terminus of the DNA contact helix formed cross-links readily, while cysteines placed at the C terminus of the helix did not.

scholarly journals Heat Shock Factor 1 (HSF1-pSer326) Predicts Response to Bortezomib-Containing Chemotherapy in Pediatric AML: A COG Study

Blood ◽  
2020 ◽  
Author(s):  
Fieke W Hoff ◽  
Anneke D van Dijk ◽  
Yi Hua Qiu ◽  
Peter P Ruvolo ◽  
Robert B Gerbing ◽  
...  
Keyword(s):  
Heat Shock ◽  
De Novo ◽  
Heat Shock Factor ◽  
Leukemic Cells ◽  
Heat Shock Factor 1 ◽  
Free Survival ◽  
Heat Shock Responses ◽  
Pediatric Aml ◽  
Hsf1 Gene

Bortezomib (BTZ) was recently evaluated in a randomized Phase 3 clinical trial which compared standard chemotherapy (cytarabine, daunorubicin, etoposide; ADE) to standard therapy with BTZ (ADEB) for de novo pediatric acute myeloid leukemia. While the study concluded that BTZ did not improve outcome overall, we examined patient subgroups benefitting from BTZ-containing chemotherapy using proteomic analyses. The proteasome inhibitor BTZ disrupts protein homeostasis and activates cytoprotective heat shock responses. We measured total heat shock factor 1 (HSF1) and phosphorylated HSF1 (HSF1-pSer326) in leukemic cells from 483 pediatric patients using Reverse Phase Protein Arrays. HSF1-pSer326 phosphorylation was significantly lower in pediatric AML compared to CD34+ non-malignant cells. We identified a strong correlation between HSF1-pSer326 expression and BTZ sensitivity. BTZ significantly improved outcome of patients with low-HSF1-pSer326 with a 5-year event-free survival of 44% (ADE) vs. 67% for low-HSF1-pSer326 treated with ADEB (P=0.019). To determine the effect of HSF1 expression on BTZ potency in vitro, cell viability with HSF1 gene variants that mimicked phosphorylated (S326A) and non-phosphorylated (S326E) HSF1-pSer326 were examined. Those with increased HSF1 phosphorylation showed clear resistance to BTZ vs. those with wild type or reduced HSF1-phosphorylation. We hypothesize that HSF1-pSer326 expression could identify patients that benefit from BTZ-containing chemotherapy.

scholarly journals Association of HSF1 Genetic Variation with Heat Tolerance in Chinese Cattle

Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1027
Author(s):  
Yu Rong ◽  
Mingfei Zeng ◽  
Xiwen Guan ◽  
Kaixing Qu ◽  
Jianyong Liu ◽  
...  
Keyword(s):  
Heat Tolerance ◽  
North China ◽  
Bos Indicus ◽  
Heat Shock Factor 1 ◽  
Heat Stress Response ◽  
Chinese Cattle ◽  
Specific Allele ◽  
Native Cattle ◽  
Humidity Index ◽  
Hsf1 Gene

The heat shock factor 1 (HSF1) gene is a regulator of the heat stress response, maximizing HSP protein expression survival. In this research, we explored the frequency distribution of a missense mutation (NC_037341.1 g.616087A > G, rs135258919) in the HSF1 gene in Chinese cattle with amino acid substitution, valine to alanine. This mutation could be related to the heat tolerance in Bos indicus. A total of 941 individuals representing 35 Chinese native cattle breeds, combining pure taurine (Angus) and indicine cattle, were used to determine the genotypes of the mutation through PCR and partial DNA sequencing. The results showed significant differences in allele frequencies and their genotypes amongst Chinese cattle from different regions. Allele G or indicine-specific allele frequency diminished from south to north China, while allele A (genotype AA) or the taurine-specific allele had a contrary pattern, which agreed with the distribution of taurine and indicine cattle. According to the association analysis, the NC_037341.1 g.616087A > G (rs135258919) of the bovine HSF1 gene, annual temperature (T), relative humidity (RH), and the temperature humidity index (THI) (p < 0.01) were interrelated closely, which indicated that the NC_037341.1 g.616087A > G of the HSF1 gene is associated with heat tolerance in indicine cattle.

scholarly journals Genetic polymorphism and expression of HSF1 gene is significantly associated with breast cancer in Saudi females

PLoS ONE ◽  
2018 ◽  
Vol 13 (3) ◽  
pp. e0193095 ◽  
Author(s):  
Sahar Almotwaa ◽  
Mohamed Elrobh ◽  
Huda AbdulKarim ◽  
Mohamed Alanazi ◽  
Sooad Aldaihan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Genetic Polymorphism ◽  
Hsf1 Gene

Translational readthrough at nonsense mutations in the HSF1 gene of Saccharomyces cerevisme

1992 ◽  
Vol 234 (3) ◽  
pp. 369-378 ◽  
Author(s):  
Jennifer B. Kopczynski ◽  
Amanda C. Raff ◽  
J. José Bonner
Keyword(s):  
Nonsense Mutations ◽  
Translational Readthrough ◽  
Hsf1 Gene

HSF1 GENE SILENCING

Pancreas ◽  
2008 ◽  
Vol 37 (4) ◽  
pp. 467-468
Author(s):  
V Dudeja ◽  
S Skube ◽  
R Chugh ◽  
Y Yokoyama ◽  
R Talukdar ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Hsf1 Gene
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