scholarly journals FGR Gene

2020 ◽  
Author(s):  
Keyword(s):  
Fgr Gene

scholarly journals Specific expression of human c-fgr in natural immunity effector cells.

1990 ◽  
Vol 10 (4) ◽  
pp. 1789-1792 ◽  
Author(s):  
K Inoue ◽  
T Yamamoto ◽  
K Toyoshima
Keyword(s):  
Blood Cells ◽  
Kinase Assay ◽  
Natural Immunity ◽  
Specific Expression ◽  
Monocytic Cells ◽  
Effector Cells ◽  
Specific Antibodies ◽  
Complex Protein ◽  
Fgr Gene ◽  
Hematopoietic Cell Lines

The c-fgr gene product was shown by immune complex protein kinase assay with specific antibodies to be a 58-kilodalton protein (p58c-fgr) with tyrosine-specific autophosphorylating activity. On examination of peripheral blood cells by immunoblotting with anti-c-fgr antibodies, p58c-fgr was found only in the fractions of monocytes, granulocytes, and natural killer cells. On the other hand, histochemical studies of hybridization demonstrated accumulation of c-fgr transcripts on most monocytes and large lymphocytes. In hematopoietic cell lines, p58c-fgr was detected in differentiated granulocytic cells as well as in differentiated monocytic cells of HL-60-cell origin. These data suggest a specific role for p58c-fgr in natural immunity effector cells.

scholarly journals Identification of fgr gene and preliminary evaluation for agronomic traits of pandan sticky rice variety (Oryza sativa L.)

2019 ◽  
Vol 02 ◽  
pp. 39-48
Author(s):  
Toan D. Pham
Keyword(s):  
Oryza Sativa ◽  
Agronomic Traits ◽  
Oryza Sativa L ◽  
Rice Variety ◽  
Rice Production ◽  
Molecular Techniques ◽  
High Yield ◽  
Preliminary Evaluation ◽  
Rice Varieties ◽  
Fgr Gene

Rice (Oryza sativa L.) is the most important food crop in Vietnam particularly in the Mekong Delta. Screening of good quality and high yield rice varieties are needed for rice production in Vietnam. The purpose of this study was to use molecular techniques to identify fgr gene and to evaluate preliminarily agronomic traits of pandan sticky rice variety. The results showed that this variety contained fgr gene. The fragrant allele was amplified by ESP - IFAP primers with a product of 255 bp in size. Similarly, evaluation of agronomic traits showed that the pandan sticky rice variety displayed many desirable characteristics such as plant height of 108 cm, panicle length of 25.6 cm, seed/panicle 135, 100-grain weight 2.07 g, pandan smell, level 2 of alkali digestion, gel consistency 93 mm. These results were useful information and could be applied for improving and providing pandan sticky rice variety for rice production.

Specific expression of human c-fgr in natural immunity effector cells

1990 ◽  
Vol 10 (4) ◽  
pp. 1789-1792
Author(s):  
K Inoue ◽  
T Yamamoto ◽  
K Toyoshima
Keyword(s):  
Blood Cells ◽  
Kinase Assay ◽  
Natural Immunity ◽  
Specific Expression ◽  
Monocytic Cells ◽  
Effector Cells ◽  
Specific Antibodies ◽  
Complex Protein ◽  
Fgr Gene ◽  
Hematopoietic Cell Lines

The c-fgr gene product was shown by immune complex protein kinase assay with specific antibodies to be a 58-kilodalton protein (p58c-fgr) with tyrosine-specific autophosphorylating activity. On examination of peripheral blood cells by immunoblotting with anti-c-fgr antibodies, p58c-fgr was found only in the fractions of monocytes, granulocytes, and natural killer cells. On the other hand, histochemical studies of hybridization demonstrated accumulation of c-fgr transcripts on most monocytes and large lymphocytes. In hematopoietic cell lines, p58c-fgr was detected in differentiated granulocytic cells as well as in differentiated monocytic cells of HL-60-cell origin. These data suggest a specific role for p58c-fgr in natural immunity effector cells.

Delimitation of the fgr gene for rice fragrance to a 28-kb DNA fragment

2009 ◽  
Vol 56 (4) ◽  
pp. 532-539 ◽  
Author(s):  
H. F. Ding ◽  
F. Y. Yao ◽  
G. X. Li ◽  
M. S. Jiang ◽  
R. F. Li ◽  
...  
Keyword(s):  
Dna Fragment ◽  
Fgr Gene

scholarly journals Genetic Analysis of Aromatic and Quality Rice Germplasm using Microsatellite Markers

2012 ◽  
Vol 22 (1) ◽  
pp. 65-71 ◽  
Author(s):  
F.D. Shams ◽  
M.M.A. Kuddus ◽  
K.M. Nasiruddin ◽  
S.N. Begum ◽  
M.M. Islam
Keyword(s):  
Genetic Analysis ◽  
Microsatellite Markers ◽  
Banding Pattern ◽  
Aromatic Rice ◽  
Good Source ◽  
Ssr Primers ◽  
Specific Allele ◽  
Rice Genotypes ◽  
Fgr Gene ◽  
High Yields

Twenty rice genotypes were analysed with three SSR primers viz., RM223, RM515 and RM342 for detection of the fgr gene responsible for aroma. The primers RM223 and RM515 identified eight and seven genotypes, respectively as having the fgr locus. Presence of the specific allele of the RM342 amplicon, also correlated with strong aroma in two and with weak aroma in five genotypes. The banding pattern for the primer RM342 was different in genotypes with no aroma. Among the three primers (RM223, RM515 and RM342), RM223 detected the highest number of fgr loci in the 20 rice genotypes. In total 11 genotypes were identified as having strong aroma genes by the three primers. These accessions also showed high yields and therefore may prove to be a good source for producing aromatic rice. DOI: http://dx.doi.org/10.3329/ptcb.v22i1.11262Plant Tissue Cult. & Biotech. 22(1): 65-71, 2012 (June)

scholarly journals Tissue-specific expression and developmental regulation of the human fgr proto-oncogene.

1989 ◽  
Vol 9 (1) ◽  
pp. 92-99 ◽  
Author(s):  
T J Ley ◽  
N L Connolly ◽  
S Katamine ◽  
M S Cheah ◽  
R M Senior ◽  
...  
Keyword(s):  
Transcriptional Activation ◽  
Developmental Regulation ◽  
Constitutive Expression ◽  
U937 Cells ◽  
Induced Differentiation ◽  
Specific Expression ◽  
Normal Peripheral Blood ◽  
Rna Molecules ◽  
Low Levels ◽  
Fgr Gene

In this study, we show that c-fgr proto-oncogene expression is limited to normal peripheral blood granulocytes, monocytes, and alveolar macrophages, all of which contain 50 to 100 copies of c-fgr mRNA per cell. The c-fgr RNA molecules in these cells consisted of partially spliced transcripts containing intron 7 and completely spliced molecules capable of encoding the predicted p55 c-fgr protein. The splicing of intron 7 appeared to occur after the splicing of most of the other introns; partially spliced molecules containing intron 7 did not appear to be transported into the cytoplasm. Very low levels of fgr transcripts were also present in U937 promonocytic cells and increased in abundance with 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced differentiation. The level of fgr transcripts began to increase 2 to 4 h after TPA addition, peaked at 8 h, and subsequently declined. Since we found that the half-life of fgr mRNA was longer than 8 h, these changes are best explained by transient transcriptional activation of fgr during TPA-induced differentiation, although nuclear runoff experiments were not sensitive enough to detect this event. Cycloheximide also caused accumulation of c-fgr transcripts in U937 cells; no superinduction was observed when TPA and cycloheximide were added at the same time. Induction by either agent was blocked with actinomycin D. These results demonstrate that the c-fgr gene is expressed in a tissue- and development-specific fashion and suggest that constitutive expression of c-fgr in U937 cells is regulated by a labile transcriptional repressor.

scholarly journals Analysis and comparison of fragrant gene sequence in some rice cultivars

Genetika ◽  
2016 ◽  
Vol 48 (2) ◽  
pp. 597-607
Author(s):  
Noushafarin Karami ◽  
Ali Aalami ◽  
Habibollah Lahiji ◽  
Babak Rabiei ◽  
Mehrzad Alahgholipour
Keyword(s):  
Amino Acids ◽  
Stop Codon ◽  
Oryza Sativa L ◽  
Chromosome 8 ◽  
Betaine Aldehyde Dehydrogenase ◽  
Rice Cultivars ◽  
Japonica Variety ◽  
Single Nucleotide ◽  
Fgr Gene ◽  
Alignment Analysis

It is known that the fragrant trait in rice (Oryza sativa L.) is largely controlled by fgr gene on chromosome 8 and it has been specified that the existence of an 8 bp deletion and three single nucleotide polymorphism (SNP) in exon 7 is effective on this trait. In this study, sequence alignment analysis of fgr exon7 on chromosome 8 for 11 different fragrant and non-fragrant cultivars revealed that 5 aromatic rice cultivars carried 3 SNPs and 8 bp deletion in exon7 which terminates prematurely at a TAA stop codon. However, 5 of the non-aromatics showed a sequence identical to the published Nipponbare, being non-fragrant Japonica variety sequence. An exception among them was Bejar, which had 8 bp deletion and 3SNPs but it was non-aromatic. Sequencing can determine nucleotide alignment of a gene and give beneficial information about gene function. In silico prediction showed proteins sequences alignment of fgr gene for Khazar and Domsiah genotypes were different. Betaine aldehyde dehydrogenase complete enzyme belongs to Khazar non-fragrant genotype that has complete length and 503 amino acids while non-functional BADH2 enzyme for Domsiah fragrant genotype has 251 amino acids that result in accumulate 2-acetyl-1-pyrroline (2AP) and produces aroma in fragrant genotypes.

Tissue-specific expression and developmental regulation of the human fgr proto-oncogene

1989 ◽  
Vol 9 (1) ◽  
pp. 92-99
Author(s):  
T J Ley ◽  
N L Connolly ◽  
S Katamine ◽  
M S Cheah ◽  
R M Senior ◽  
...  
Keyword(s):  
Transcriptional Activation ◽  
Developmental Regulation ◽  
Constitutive Expression ◽  
U937 Cells ◽  
Induced Differentiation ◽  
Specific Expression ◽  
Normal Peripheral Blood ◽  
Rna Molecules ◽  
Low Levels ◽  
Fgr Gene

In this study, we show that c-fgr proto-oncogene expression is limited to normal peripheral blood granulocytes, monocytes, and alveolar macrophages, all of which contain 50 to 100 copies of c-fgr mRNA per cell. The c-fgr RNA molecules in these cells consisted of partially spliced transcripts containing intron 7 and completely spliced molecules capable of encoding the predicted p55 c-fgr protein. The splicing of intron 7 appeared to occur after the splicing of most of the other introns; partially spliced molecules containing intron 7 did not appear to be transported into the cytoplasm. Very low levels of fgr transcripts were also present in U937 promonocytic cells and increased in abundance with 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced differentiation. The level of fgr transcripts began to increase 2 to 4 h after TPA addition, peaked at 8 h, and subsequently declined. Since we found that the half-life of fgr mRNA was longer than 8 h, these changes are best explained by transient transcriptional activation of fgr during TPA-induced differentiation, although nuclear runoff experiments were not sensitive enough to detect this event. Cycloheximide also caused accumulation of c-fgr transcripts in U937 cells; no superinduction was observed when TPA and cycloheximide were added at the same time. Induction by either agent was blocked with actinomycin D. These results demonstrate that the c-fgr gene is expressed in a tissue- and development-specific fashion and suggest that constitutive expression of c-fgr in U937 cells is regulated by a labile transcriptional repressor.

The fgr gene responsible for rice fragrance was restricted within 69kb

Plant Science ◽  
2006 ◽  
Vol 171 (4) ◽  
pp. 505-514 ◽  
Author(s):  
Saihua Chen ◽  
Juan Wu ◽  
Yi Yang ◽  
Weiwei Shi ◽  
Mingliang Xu
Keyword(s):  
Fgr Gene
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