scholarly journals Amiloride Hydrochloride

2020 ◽  
Author(s):  
Keyword(s):  
Amiloride Hydrochloride

A New Application of Continuous Wavelet Transform to Overlapping Chromatograms for the Quantitative Analysis of Amiloride Hydrochloride and Hydrochlorothiazide in Tablets by Ultra-Performance Liquid Chromatography

2012 ◽  
Vol 95 (3) ◽  
pp. 751-756 ◽  
Author(s):  
Erdal Dinç ◽  
Eda Büker
Keyword(s):  
Signal Processing ◽  
Wavelet Transform ◽  
Quantitative Analysis ◽  
Continuous Wavelet Transform ◽  
Standard Addition ◽  
Ultra Performance Liquid Chromatography ◽  
Continuous Wavelet ◽  
Constant Flow Rate ◽  
Acquity Uplc ◽  
Amiloride Hydrochloride

Abstract A new application of continuous wavelet transform (CWT) to overlapping peaks in a chromatogram was developed for the quantitative analysis of amiloride hydrochloride (AML) and hydrochlorothiazide (HCT) in tablets. Chromatographic analysis was done by using an ACQUITY ultra-performance LC (UPLC) BEH C18 column (50 × 2.1 mm id, 1.7 μm particle size) and a mobile phase consisting of methanol–0.1 M acetic acid (21 + 79, v/v) at a constant flow rate of 0.3 mL/min with diode array detection at 274 nm. The overlapping chromatographic peaks of the calibration set consisting of AML and HCT mixtures were recorded rapidly by using an ACQUITY UPLC H-Class system. The overlapping UPLC data vectors of AML and HCT drugs and their samples were processed by CWT signal processing methods. The calibration graphs for AML and HCT were computed from the relationship between concentration and areas of chromatographic CWT peaks. The applicability and validity of the improved UPLC-CWT approaches were confirmed by recovery studies and the standard addition technique. The proposed UPLC-CWT methods were applied to the determination of AML and HCT in tablets. The experimental results indicated that the suggested UPLC-CWT signal processing provides accurate and precise results for industrial QC and quantitative evaluation of AML-HCT tablets.

The metabolism of amiloride hydrochloride in man

1969 ◽  
Vol 10 (3) ◽  
pp. 401-406 ◽  
Author(s):  
Peter Weiss ◽  
Roscoe M. Hersey ◽  
Carlos A. Dujovne ◽  
Joseph R. Bianchine
Keyword(s):  
Amiloride Hydrochloride

Amiloride-sensitive sodium channels and expression of sodium appetite in rats

1987 ◽  
Vol 253 (2) ◽  
pp. R371-R374 ◽  
Author(s):  
I. L. Bernstein ◽  
C. J. Hennessy
Keyword(s):  
Sodium Channels ◽  
Transport System ◽  
Sodium Transport ◽  
Taste Bud ◽  
Sodium Balance ◽  
Gustatory System ◽  
Sodium Appetite ◽  
Amiloride Hydrochloride

Lingual application of amiloride hydrochloride blocks a sodium transport system in the mammalian gustatory system. Effects of exposure to amiloride on subsequent licking for 3% NaCl by rats were found to differ as a function of the animal's sodium balance. Licking for 3% NaCl was significantly increased in sodium-replete rats and significantly decreased in sodium-deplete rats by amiloride pretreatment. In fact, expression of sodium appetite was virtually eliminated by pretreatment with amiloride. This suggests that the recognition of sodium solutions in animals with a sodium deficit is dependent on amiloride-sensitive sodium transport at the taste bud.

scholarly journals Development and Validation of a Reversed-Phase High-Performance Liquid Chromatographic Method for the Simultaneous Determination of Amiloride Hydrochloride, Atenolol, Hydrochlorothiazide, and Chlorthalidone in Their Combined Mixtures

2009 ◽  
Vol 92 (2) ◽  
pp. 404-409 ◽  
Author(s):  
Abdalla A Elshanawane ◽  
Samia M Mostafa ◽  
Mohamed S Elgawish
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
Chromatographic Method ◽  
High Performance Liquid Chromatographic ◽  
Ternary Mixtures ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatographic ◽  
Amiloride Hydrochloride

Abstract A high-performance liquid chromatographic method was developed for the simultaneous determination of 2 ternary mixtures containing amiloride hydrochloride, atenolol, hydrochlorothiazide, and chlorthalidone used in hypertension therapy. The use of cyanopropyl column results in satisfactory separation of both mixtures. The mobile phase consisted of 10 mM KH2PO4 buffer (pH 4.5) and methanol in a ratio of (75 25 v/v), at a flow rate of 1 mL/min. UV detector was operated at 275 nm. Calibration graphs were linear in the concentration ranges of 210, 20200, 10100, and 550 g/mL for amiloride hydrochloride, atenolol, hydrochlorothiazide, and chlorthalidone, respectively. Intraday and interday precision values (relative standard deviation) were <1.13 for mixture I (amiloride hydrochloride, atenolol, chlorthalidone), and <0.93 for mixture II (amiloride hydrochloride, atenolol, hydrochlorothiazide). The method was successfully applied for the determination of the 2 combinations in laboratory-prepared mixtures and commercial pharmaceutical formulation with high accuracy and precision. Statistical comparison of the results with those of the published methods showed excellent agreement and indicates no significant difference between them.

AMILORIDE HYDROCHLORIDE, A HYPERCALCIURIC DIURETIC

1969 ◽  
Vol 18 (3) ◽  
pp. 267-270 ◽  
Author(s):  
K. V. JOHNY ◽  
J. R. LAWRENCE ◽  
M. W. O'HALLORAN
Keyword(s):  
Amiloride Hydrochloride

Amiloride sensitivity of chorda tympani response to NaCl in Fischer 344 and Wistar rats

1991 ◽  
Vol 261 (2) ◽  
pp. R329-R333 ◽  
Author(s):  
I. L. Bernstein ◽  
A. Longley ◽  
E. M. Taylor
Keyword(s):  
Sodium Transport ◽  
Wistar Rats ◽  
Strain Differences ◽  
Chorda Tympani ◽  
Gustatory System ◽  
Electrophysiological Response ◽  
Fischer 344 ◽  
Electrophysiological Responses ◽  
Before And After ◽  
Amiloride Hydrochloride

Fischer 344 (F-344) rats fail to prefer NaCl solutions to water at any concentration and avoid NaCl solutions preferred by other strains, including Wistar rats. Behavioral and electrophysiological responses of the mammalian gustatory system to NaCl have been shown to depend on a sodium transport system that is specifically blocked by lingual application of the sodium-transport blocker amiloride. The present study examined whether strain differences exist between F-344 and Wistar rats in the amiloride sensitivity of the chorda tympani (CT) electrophysiological response to NaCl. Whole nerve CT recordings were obtained from adult F-344 and Wistar rats during chemical stimulation of the anterior tongue. Responses to NaCl solutions ranging from 0.01 to 1.0 M were examined both before and after pretreatment with amiloride hydrochloride. Integrated whole nerve responses to NaCl solutions were expressed relative to the response to 0.5 M NH4Cl. Strain differences in the response to NaCl solutions emerged, with F-344 animals showing a significantly larger amplitude of the tonic response to NaCl, relative to NH4Cl, than Wistars. F-344 rats were also more sensitive to the sodium-channel blocker amiloride. These results suggest that strain differences in amiloride sensitive signals mediated by the CT nerve may contribute to the NaCl aversion displayed by F-344 rats.

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