scholarly journals HAAH Lambda phage Vaccine SNS-301

2020 ◽  
Author(s):  
Keyword(s):  
Lambda Phage

An Action Spectrum of the Riboflavin Photosensitized Inactivation of Lambda Phage

2004 ◽  
Author(s):  
Christopher B. Martin ◽  
Erin Wilfong ◽  
Patrick Ruane ◽  
Raymond Goodrich ◽  
Matthew Platz
Keyword(s):  
Action Spectrum ◽  
Lambda Phage ◽  
Photosensitized Inactivation

scholarly journals Further tests of a recombination model in which chi removes the RecD subunit from the RecBCD enzyme of Escherichia coli.

Genetics ◽  
1990 ◽  
Vol 126 (3) ◽  
pp. 519-533 ◽  
Author(s):  
F W Stahl ◽  
L C Thomason ◽  
I Siddiqi ◽  
M M Stahl
Keyword(s):  
Escherichia Coli ◽  
Dna Sequence ◽  
Dna Packaging ◽  
Lambda Phage ◽  
Recombination Model ◽  
Phage Types ◽  
Point Of Entry ◽  
Reciprocal Recombination ◽  
Recbcd Enzyme ◽  
Lambda Dna

Abstract When one of two infecting lambda phage types in a replication-blocked cross is chi + and DNA packaging is divorced from the RecBCD-chi interaction, complementary chi-stimulated recombinants are recovered equally in mass lysates only if the chi + parent is in excess in the infecting parental mixture. Otherwise, the chi 0 recombinant is recovered in excess. This observation implies that, along with the chi 0 chromosome, two chi + parent chromosomes are involved in the formation of each chi + recombinant. The trimolecular nature of chi +-stimulated recombination is manifest in recombination between lambda and a plasmid. When lambda recombines with a plasmid via the RecBCD pathway, the resulting chromosome has an enhanced probability of undergoing lambda x lambda recombination in the interval into which the plasmid was incorporated. These two observations support a model in which DNA is degraded by Exo V from cos, the sequence that determines the end of packaged lambda DNA and acts as point of entry for RecBCD enzyme, to chi, the DNA sequence that stimulates the RecBCD enzyme to effect recombination. The model supposes that chi acts by ejecting the RecD subunit from the RecBCD enzyme with two consequences. (1) ExoV activity is blocked leaving a highly recombinagenic, frayed duplex end near chi, and (2) as the enzyme stripped of the RecD subunit travels beyond chi it is competent to catalyze reciprocal recombination.

scholarly journals Immunogenicity of a lambda phage-based anti-cancer vaccine targeting HAAH

2013 ◽  
Vol 1 (Suppl 1) ◽  
pp. P210 ◽  
Author(s):  
Steven Fuller ◽  
Solomon Stewart ◽  
Michael Lebowitz ◽  
Kanam Malhotra ◽  
Mark Semenuk ◽  
...  
Keyword(s):  
Cancer Vaccine ◽  
Lambda Phage ◽  
Anti Cancer

scholarly journals Membrane and Cytoplasmic Proteins of Mycobacterium avium subspecies paratuberculosis that Bind to Novel Monoclonal Antibodies

2018 ◽  
Vol 6 (4) ◽  
pp. 127 ◽  
Author(s):  
John Bannantine ◽  
Judith Stabel ◽  
John Lippolis ◽  
Timothy Reinhardt
Keyword(s):  
Monoclonal Antibodies ◽  
Mycobacterium Avium ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Cell Extract ◽  
Johne's Disease ◽  
Johne’S Disease ◽  
Mass Spectrometry Analysis ◽  
Lambda Phage ◽  
Expression Library ◽  
Spectrometry Analysis

Monoclonal antibodies against Mycobacterium avium subspecies paratuberculosis (Map) proteins are important tools in Johne’s disease research and diagnostics. Johne’s disease is a chronic inflammatory intestinal disease of cattle, sheep, and other ruminant animals. We have previously generated multiple sets of monoclonal antibodies (mAbs) in different studies; however, because many were generated and screened against a whole-cell extract of Map, the antigens that bind to these antibodies remained unknown. In this study, we used three different approaches to identify the corresponding Map antigens for 14 mAbs that could not be identified previously. In the first approach, a new Map-lambda phage expression library was screened to identify corresponding antigens for 11 mAbs. This approach revealed that mAbs 7C8, 9H3, 12E4, 3G5, and 11B8 all detect MAP_3404 encoding the biotin carboxylase subunit of acetyl-CoA carboxylase, while mAbs 7A6, 11F8, and 10C12 detect the GroEL2 chaperonin (MAP_3936), 6C9 detects electron transfer flavoprotein (MAP_3060c), and 14G11 detects MAP_3976, a lipoprotein anchoring transpeptidase. The epitopes to a selection of these mAbs were also defined. In a second approach, MAP_2698c bound monoclonal antibody (mAb) 14D4 as determined using protein arrays. When both of these approaches failed to identify the antigen for mAb 12C9, immunoprecipitation, mass spectrometry analysis, and codon optimization was used to identify the membrane protein, MAP_4145, as the reacting antigen. Characterized antibodies were used to quickly interrogate mycobacterial proteomic preps. We conclude by providing a complete catalog of available mAbs to Map proteins, along with their cognate antigens and epitopes, if known. These antibodies are now thoroughly characterized and more useful for research and diagnostic purposes.

Genetic selection of lambda phage clones from a gene clonotheque

1986 ◽  
Vol 203 (2) ◽  
pp. 312-315
Author(s):  
Anatolij A. Melnikov ◽  
Istvan Fodor
Keyword(s):  
Genetic Selection ◽  
Lambda Phage ◽  
Selection Of

Construction of a Genomic Library in Lambda Phage

1994 ◽  
pp. 595-598
Author(s):  
Clifford F. Weil ◽  
Thomas E. Bureau
Keyword(s):  
Genomic Library ◽  
Lambda Phage

scholarly journals Dense display of HIV-1 envelope spikes on the lambda phage scaffold does not result in the generation of improved antibody responses to HIV-1 Env

Vaccine ◽  
2011 ◽  
Vol 29 (14) ◽  
pp. 2637-2647 ◽  
Author(s):  
Jonelle Mattiacio ◽  
Scott Walter ◽  
Matt Brewer ◽  
William Domm ◽  
Alan E. Friedman ◽  
...  
Keyword(s):  
Antibody Responses ◽  
Lambda Phage ◽  
Hiv 1

Lambda phage DNA sequences affecting the packaging process

Gene ◽  
1983 ◽  
Vol 24 (2-3) ◽  
pp. 199-206 ◽  
Author(s):  
Miwa Takeshi ◽  
Matsubara Kenichi
Keyword(s):  
Dna Sequences ◽  
Lambda Phage ◽  
Packaging Process ◽  
Phage Dna
Sign in / Sign up

Export Citation Format

Share Document