scholarly journals BB, Rat Strain

2020 ◽  
Author(s):  
Keyword(s):  
Bb Rat ◽  
Rat Strain

scholarly journals Nitric Oxide Is a Mediator of Antiproliferative Effects Induced by Proinflammatory Cytokines on Pancreatic Beta Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Laura Quintana-Lopez ◽  
Manuel Blandino-Rosano ◽  
Gonzalo Perez-Arana ◽  
Alberto Cebada-Aleu ◽  
Alfonso Lechuga-Sancho ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Proinflammatory Cytokines ◽  
Pancreatic Beta Cells ◽  
Antiproliferative Effect ◽  
Bb Rat ◽  
Antiproliferative Effects ◽  
Initial Stage ◽  
Rat Strain ◽  
Nos Inhibitor

Nitric oxide (NO) is involved in several biological processes. In type 1 diabetes mellitus (T1DM), proinflammatory cytokines activate an inducible isoform of NOS (iNOS) inβcells, thus increasing NO levels and inducing apoptosis. The aim of the current study is to determine the role of NO (1) in the antiproliferative effect of proinflammatory cytokines IL-1β, IFN-γ, and TNF-αon cultured isletβcells and (2) during the insulitis stage prior to diabetes onset using the Biobreeding (BB) rat strain as T1DM model. Our results indicate that NO donors exert an antiproliferative effect onβcell obtained from cultured pancreatic islets, similar to that induced by proinflammatory cytokines. This cytokine-induced antiproliferative effect can be reversed by L-NMMA, a general NOS inhibitor, and is independent of guanylate cyclase pathway. Assays using NOS isoform specific inhibitors suggest that the NO implicated in the antiproliferative effect of proinflammatory cytokines is produced by inducible NOS, although not in an exclusive way. In BB rats, early treatment with L-NMMA improves the initial stage of insulitis. We conclude that NO is an important mediator of antiproliferative effect induced by proinflammatory cytokines on culturedβcell and is implicated inβ-cell proliferation impairment observed early from initial stage of insulitis.

BB.LL – a newly established congenic BB rat strain

2009 ◽  
Vol 105 (S 03) ◽  
pp. 80-81
Author(s):  
Birger Voigt ◽  
Peter Kovacs ◽  
Ingrid Klöting
Keyword(s):  
Bb Rat ◽  
Rat Strain

The influence of T-lymphocyte precursor cells and thymus grafts on the cellular immunodeficiencies of the BB rat

Diabetes ◽  
1985 ◽  
Vol 34 (11) ◽  
pp. 1134-1138 ◽  
Author(s):  
J. W. Francfort ◽  
A. Naji ◽  
W. K. Silvers ◽  
C. F. Barker
Keyword(s):  
T Lymphocyte ◽  
Precursor Cells ◽  
Bb Rat

Peculiar T-cell signaling does not preclude positive selection in the diabetes-prone BB rat

Diabetes ◽  
1994 ◽  
Vol 43 (1) ◽  
pp. 47-52 ◽  
Author(s):  
D. Bellgrau ◽  
J. M. Redd ◽  
K. S. Sellins
Keyword(s):  
T Cell ◽  
Cell Signaling ◽  
Positive Selection ◽  
Bb Rat ◽  
T Cell Signaling

The onset and progression of pancreatic insulitis in the overt, spontaneously diabetic, young adult BB rat studied by pancreatic biopsy

Diabetes ◽  
1984 ◽  
Vol 33 (1) ◽  
pp. 33-36 ◽  
Author(s):  
J. Logothetopoulos ◽  
N. Valiquette ◽  
E. Madura ◽  
D. Cvet
Keyword(s):  
Young Adult ◽  
Bb Rat ◽  
Pancreatic Biopsy

scholarly journals Rat strain differences in pilocarpine-induced mouse killing

1976 ◽  
Vol 4 (1) ◽  
pp. 28-32 ◽  
Author(s):  
Patricia E. Gay ◽  
Russell C. Leaf
Keyword(s):  
Strain Differences ◽  
Mouse Killing ◽  
Rat Strain

POSSIBLE ROLE OF 5α-ANDROSTANE-3α,17β-DIOL IN THE CONTROL OF FOLLICLE-STIMULATING HORMONE SECRETION IN THE IMMATURE FEMALE RAT

1982 ◽  
Vol 92 (1) ◽  
pp. 37-42 ◽  
Author(s):  
H. M. A. MEIJS-ROELOFS ◽  
P. KRAMER ◽  
L. GRIBLING-HEGGE
Keyword(s):  
Inhibitory Action ◽  
Combined Treatment ◽  
Hormone Secretion ◽  
Inhibitory Effect ◽  
Ovariectomized Rats ◽  
Female Rat ◽  
Immature Rat ◽  
Rat Strain ◽  
Intact Rats

A possible role of 5α-androstane-3α,17β-diol (3α-androstanediol) in the control of FSH secretion was studied at various ages in ovariectomized rats. In the rat strain used, vaginal opening, coincident with first ovulation, generally occurs between 37 and 42 days of age. If 3α-androstanediol alone was given as an ovarian substitute, an inhibitory effect on FSH release was evident with all three doses tested (50, 100, 300 μg/100 g body wt) between 13 and 30 days of age; at 33–35 days of age only the 300 μg dose caused some inhibition of FSH release. Results were more complex if 3α-androstanediol was given in combined treatment with oestradiol and progesterone. Given with progesterone, 3α-androstanediol showed a synergistic inhibitory action on FSH release between 20 and 30 days of age. However, when 3α-androstanediol was combined with oestradiol a clear decrease in effect, as compared to the effect of oestradiol alone, was found between 20 and 30 days of age. Also the effect of combined oestradiol and progesterone treatment was greater than the effect of combined treatment with oestradiol, progesterone and 3α-androstanediol. At all ages after day 20 none of the steroid combinations tested was capable of maintaining FSH levels in ovariectomized rats similar to those in intact rats. It is concluded that 3α-androstanediol might play a role in the control of FSH secretion in the immature rat, but after day 20 the potentially inhibitory action of 3α-androstanediol on FSH secretion is limited in the presence of oestradiol.

scholarly journals New homozygous gpt delta transgenic rat strain improves an efficiency of the in vivo mutagenicity assay

2021 ◽  
Vol 43 (1) ◽  
Author(s):  
Kenichi Masumura ◽  
Tomoko Ando ◽  
Akiko Ukai ◽  
Sho Fujiwara ◽  
Shigeo Yokose ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Gene Mutation ◽  
Point Mutations ◽  
Chromosome 1 ◽  
Transgenic Rat ◽  
Target Tissue ◽  
Sequencing Analysis ◽  
Rat Strain ◽  
Packaging Efficiency

Abstract Background Gene mutation assays in transgenic rodents are useful tools to investigate in vivo mutagenicity in a target tissue. Using a lambda EG10 transgene containing reporter genes, gpt delta transgenic mice and rats have been developed to detect point mutations and deletions. The transgene is integrated in the genome and can be rescued through an in vitro packaging reaction. However, the packaging efficiency is lower in gpt delta rats than in mice, because of the transgene in gpt delta rats being heterozygous and in low copy number. To improve the packaging efficiency, we herein describe a newly developed homozygous gpt delta rat strain. Results The new gpt delta rat has a Wistar Hannover background and has been successfully maintained as homozygous for the transgene. The packaging efficiency in the liver was 4 to 8 times higher than that of existing heterozygous F344 gpt delta rats. The frequency of gpt point mutations significantly increased in the liver and bone marrow of N-nitroso-N-ethylurea (ENU)- and benzo[a]pyrene (BaP)-treated rats. Spi− deletion frequencies significantly increased in the liver and bone marrow of BaP-treated rats but not in ENU-treated rats. Whole genome sequencing analysis identified ≥ 30 copies of lambda EG10 transgenes integrated in rat chromosome 1. Conclusions The new homozygous gpt delta rat strain showed a higher packaging efficiency, and could be useful for in vivo gene mutation assays in rats.

Sign in / Sign up

Export Citation Format

Share Document