Microbial Culture Procedure

2020 ◽  
Author(s):  
Keyword(s):  
Microbial Culture ◽  
Culture Procedure

Effect of nickel(II) on the biomass yield of the activated sludge

1996 ◽  
Vol 34 (5-6) ◽  
pp. 163-171 ◽  
Author(s):  
Celal F. Gökçay ◽  
Ulku Yetis
Keyword(s):  
Activated Sludge ◽  
Decay Constant ◽  
Biomass Yield ◽  
Culture Conditions ◽  
Synthetic Wastewater ◽  
Base Line ◽  
Microbial Culture ◽  
Feed Composition ◽  
Steady State Condition ◽  
Applied Microbiology

Biomass yield of microorganisms is important in applied microbiology since it is the ultimate factor determining the amount of product produced regardless of whether product is growth-linked or not. In the case of environmental microbiology the opposite is true and minimizing the biomass produced, or the sludge in the relevant jargon, often is the prime goal. In this paper, a unique means of manipulating the microbial biomass yield of a heterogeneous culture to fulfil either of the two goals is presented. 5.0 mgl−1 Ni(II) in the feed composition to a completely mixed, once- through, activated sludge was found to induce the observed biomass yield of the microbial culture developed from sewage. As compared with the base-line study without Ni(II), where the reactor received synthetic wastewater only, true biomass yield was found to have increased along with the increased decay constant with the net effect of lowering observed biomass yield drastically at lower dilution rates and increasing it over that observed in the base-line study at higher dilution rates. At 10.0 mgl−1 influent Ni(II) concentration the culture conditions almost reverted back to the base- line study and at 25 mgl−1 Ni(II) concentration a truly steady-state condition could not be attained.

Minimization of activated sludge production by chemically stimulated energy spilling

2000 ◽  
Vol 42 (12) ◽  
pp. 189-200 ◽  
Author(s):  
G.-H. Chen ◽  
H.-K. Mo ◽  
S. Saby ◽  
W.-k. Yip ◽  
Y. Liu
Keyword(s):  
Activated Sludge ◽  
Growth Yield ◽  
Dry Weight ◽  
Staining Technique ◽  
Microbial Culture ◽  
Dapi Staining ◽  
E Coli ◽  
Energy Spilling ◽  
Activated Sludge Processes ◽  
Sludge Production

Minimization of excess sludge production in activated sludge processes has been pursued around the world in order to meet stringent environmental regulations on sludge treatment and disposal. To achieve this goal, physical, chemical, and biological approaches have been proposed. In this paper, a chemical compound, 3,3′,4′,5-tetrachlorosalicylanilide (TCS) was tested for enhancing microbial energy spilling of the sludgeso as to minimize its growth. In order to examine this, an exploratory study was conducted using both batch and continuous activated sludge cultures. Batch experiments with these two cultures were carried out at different initial concentrations of TCS. It has been confirmed that an addition of TCS is effective in reducing the production of both the sludge cultures, particularly the continuous culture where the observed growth yield was reduced by around 70%, when the initial TCS concentration was 0.8 ppm. Meanwhile, the substrate removal activity of this culture was found not to be affected at this TCS concentration. To further evaluate the TCS effect, a pure microbial culture of E. coli was employed. Batch experiment results with this culture implied that TCS might be able to reduce the cell density of E. coli drastically when an initial TCS concentration was greater than 0.12 ppm. It was also found that TCS was not toxic to this type of bacteria. Microscopic examinations with a 4′, 6-diamidino-2-phenylindole (DAPI) staining technique revealed that TCS neither affected the cell division nor altered the cell size of E. coli. However, both the cell ATP content and the cell dry weight were reduced significantly with the addition of TCS.

Characterization of Nostoc muscorum NCCU-442 Derived Poly-3- hydroxybutyrate (PHB) and Polyethylene Glycol (PEG) Blends

2020 ◽  
Vol 10 (3) ◽  
pp. 200-207
Author(s):  
Sabbir Ansari ◽  
Tasneem Fatma
Keyword(s):  
Thermal Properties ◽  
Polyethylene Glycol ◽  
Morphological Properties ◽  
Morphological Alteration ◽  
Nostoc Muscorum ◽  
Microbial Culture ◽  
Polymer Science ◽  
Mixed Microbial Culture ◽  
Scanning Calorimetry ◽  
Casting Technique

Background: Poly-3-hydroxybutyrate (PHB) has attracted much consideration as biodegradable biocompatible polymer. This thermoplastic polymer has comparable material properties to polypropylene. Materials with more valuable properties may result from blending, a common practice in polymer science. Objective: In this paper, blends of PHB (extracted from cyanobacterium Nostoc muscorum NCCU- 442 with polyethylene glycol (PEG) were investigated for their thermal, tensile, hydrophilic and biodegradation properties. Methods: Blends were prepared in different proportions of PHB/PEG viz. 100/0, 98/2, 95/5, 90/10, 80/20, and 70/30 (wt %) using solvent casting technique. Morphological properties were investigated by using Scanning Electron Microscopy (SEM). Differential scanning calorimetry and thermogravimetric analysis were done for thermal properties determination whereas the mechanical and hydrophilic properties of the blends were studied by means of an automated material testing system and contact angle analyser respectively. Biodegradability potential of the blended films was tested as percent weight loss by mixed microbial culture within 60 days. Results: The blends showed good misciblity between PEG and PHB, however increasing concentrations of plasticizer caused morphological alteration as evidenced by SEM micrographs. PEG addition (10 % and above) showed significant alternations in the thermal properties of the blends. Increase in the PEG content increased the elongation at break ratio i.e enhanced the required plasticity of PHB. Rate of microbial facilitated degradation of the blends was greater with increasing PEG concentrations. Conclusion: Blending with PEG increased the crucial polymeric properties of cyanobacterial PHB.

scholarly journals Bloodstream infection with Acinetobacter baumanii in a Plasmodium falciparum positive infant: a case report

2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Charity Wiafe Akenten ◽  
Kennedy Gyau Boahen ◽  
Kwadwo Sarfo Marfo ◽  
Nimako Sarpong ◽  
Denise Dekker ◽  
...  
Keyword(s):  
Case Report ◽  
Bacterial Infections ◽  
Treatment Options ◽  
Female Child ◽  
Correct Choice ◽  
Blood Cultures ◽  
Microbial Culture ◽  
Acinetobacter Baumanii ◽  
Inappropriate Use ◽  
History Of

Abstract Background The increasing incidence of multi-antibiotic-resistant bacterial infections, coupled with the risk of co-infections in malaria-endemic regions, complicates accurate diagnosis and prolongs hospitalization, thereby increasing the total cost of illness. Further, there are challenges in making the correct choice of antibiotic treatment and duration, precipitated by a lack of access to microbial culture facilities in many hospitals in Ghana. The aim of this case report is to highlight the need for blood cultures or alternative rapid tests to be performed routinely in malaria patients, to diagnose co-infections with bacteria, especially when symptoms persist after antimalarial treatment. Case presentation A 6-month old black female child presented to the Agogo Presbyterian Hospital with fever, diarrhea, and a 3-day history of cough. A rapid diagnostic test for malaria and Malaria microscopy was positive for P. falciparum with a parasitemia of 224 parasites/μl. The patient was treated with Intravenous Artesunate, parental antibiotics (cefuroxime and gentamicin) and oral dispersible zinc tablets in addition to intravenous fluids. Blood culture yielded Acinetobacter baumanii, which was resistant to all of the third-generation antibiotics included in the susceptibility test conducted, but sensitive to ciprofloxacin and gentamicin. After augmenting treatment with intravenous ciprofloxacin, all symptoms resolved. Conclusion Even though this study cannot confirm whether the bacterial infection was nosocomial or otherwise, the case highlights the necessity to test malaria patients for possible co-infections, especially when fever persists after parasites have been cleared from the bloodstream. Bacterial blood cultures and antimicrobial susceptibility testing should be routinely performed to guide treatment options for febril illnesses in Ghana in order to reduce inappropriate use of broad-spectrum antibiotics and limit the development of antimicrobial resistance.

scholarly journals Microbial Culture Preservation With Silica Gel

1969 ◽  
Vol 58 (3) ◽  
pp. 423-425 ◽  
Author(s):  
A. R. GRIVELL ◽  
J. F. JACKSON
Keyword(s):  
Silica Gel ◽  
Microbial Culture

scholarly journals COLMENA: A Culture Collection of Native Microorganisms for Harnessing the Agro-Biotechnological Potential in Soils and Contributing to Food Security

Diversity ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 337
Author(s):  
Sergio de los Santos-Villalobos ◽  
Alondra María Díaz-Rodríguez ◽  
María Fernanda Ávila-Mascareño ◽  
Andrea Denisse Martínez-Vidales ◽  
Fannie Isela Parra-Cota
Keyword(s):  
Food Security ◽  
Plant Growth ◽  
Field Trials ◽  
Culture Collection ◽  
Microbial Culture ◽  
Biotechnological Potential ◽  
Global Food Security ◽  
Beneficial Effects ◽  
Plant Growth Promoting

COLMENA is a microbial culture collection dedicated to the characterization, classification, preservation, and transferal of native microorganisms isolated from various agro-systems and other ecosystems in Mexico. This collection aims to protect microbial diversity, reducing soil degradation, but also exploiting its agro-biotechnological potential. So far, COLMENA has isolated and cryopreserved soil microorganisms from different crops in two major agricultural regions in Mexico, the Yaqui Valley, Sonora, and the Fuerte Valley, Sinaloa. COLMENA has specialized in the identification and characterization of microbial strains with metabolic capacities related to the promotion of plant growth and the biocontrol of phytopathogens. Thus, COLMENA has identified several promising plant growth-promoting microbial (PGPM) strains due to their metabolic and genetic potentials and their beneficial effects in vivo and field trials. These findings demonstrate the biotechnological potential of these strains for their future use in profitable agricultural alternatives focused on enhancing global food security. To share the knowledge and results of the COLMENA team’s scientific research, a virtual platform was created, where the database of the studied and preserved microorganisms is available to professionals, researchers, agricultural workers, and anyone who is interested.

scholarly journals Evaluation of Microbial Culture Techniques for the Isolation of Pythium Insidiosum from Equine Tissues

2002 ◽  
Vol 14 (4) ◽  
pp. 288-294 ◽  
Author(s):  
Amy M. Grooters ◽  
Amy Whittington ◽  
Mae K. Lopez ◽  
Michelle N. Boroughs ◽  
Alma F. Roy
Keyword(s):  
Room Temperature ◽  
Microbial Culture ◽  
Sample Type ◽  
Pythium Insidiosum ◽  
Selective Media ◽  
Culture Techniques ◽  
Media Type ◽  
Antibiotic Solution ◽  
And Storage ◽  
Storage Technique

The purpose of this study was to evaluate the effects of sample handling, storage, and culture techniques on the isolation of Pythium insidiosum from infected equine tissues. Tissue and kunker samples obtained immediately posteuthanasia from a horse with subcutaneous pythiosis were used to assess the effects of sample type (kunkers vs. tissues), media type (selective vs. nonselective), storage technique, and storage time on P. insidiosum isolation rate. Overall, isolation rates were higher from fresh kunkers (94.6%) and stored kunkers (76.4%) than from fresh tissues (8.3%) or stored tissues (4.6%). Isolation of P. insidiosum also occurred more often on antibiotic-containing media than on nonselective media for both fresh and stored samples. For samples that were stored for 1–3 days prior to culture, P. insidiosum isolation rates were highest for the following techniques: kunkers stored at room temperature and plated on selective media (100%), kunkers stored at 4 C and then plated on either nonselective (91.7%) or selective (95.8%) media, kunkers stored on cold packs and then plated on either nonselective (93.8%) or selective (100%) media, kunkers stored in ampicillin solution and plated on selective media (100%), and kunkers stored in ampicillin/gentocin solution and plated on selective media (87.5%). For samples stored for 4–5 days, P. insidiosum isolation rates were highest for kunkers stored at 4 C and then plated on either nonselective (81.3%) or selective (87.5%) media, kunkers stored in ampicillin solution and then plated on selective media (87.5%), and kunkers stored in ampicillin/gentocin solution and plated on selective media (87.5%). Results of this study suggest that optimal isolation rates of P. insidiosum from infected equine tissues are achieved by culturing fresh kunkers on selective media. For samples that cannot be processed immediately, acceptable handling techniques include storage at room temperature for up to 3 days, refrigeration for up to 5 days, shipping on cold packs, and storage in antibiotic solution, each combined with subsequent inoculation on selective media.

Sign in / Sign up

Export Citation Format

Share Document