scholarly journals EWSR1/ATF1 Fusion Gene Type 1

2020 ◽  
Author(s):  
Keyword(s):  
Fusion Gene ◽  
Gene Type

Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene

1991 ◽  
Vol 11 (6) ◽  
pp. 3374-3378 ◽  
Author(s):  
S D Lupton ◽  
L L Brunton ◽  
V A Kalberg ◽  
R W Overell
Keyword(s):  
Thymidine Kinase ◽  
Negative Selection ◽  
Fusion Gene ◽  
Hygromycin B ◽  
Thymidine Kinase Gene ◽  
Kinase Gene ◽  
Hygromycin Phosphotransferase ◽  
Simplex Virus ◽  
Genetic Entity

The hygromycin phosphotransferase gene was fused in-frame with the herpes simplex virus type 1 thymidine kinase gene. The resulting fusion gene (termed HyTK) confers hygromycin B resistance for dominant positive selection and ganciclovir sensitivity for negative selection and provides a means by which these selectable phenotypes may be expressed and regulated as a single genetic entity.

Molecular Characterization of Velogenic Avian Avulavirus Type 1 Isolated from Apparently Healthy Emu Birds: Implications to Viral Maintenance and Spread

2021 ◽  
Author(s):  
B. Deepthi ◽  
D. Ratnamma ◽  
R.N. Ramani Pushpa ◽  
Shrikrishna Isloor ◽  
B.M. Veeregowda ◽  
...  
Keyword(s):  
Fusion Gene ◽  
Mrna Levels ◽  
Infected Chicken ◽  
Cytokine Mrna ◽  
Experimental Inoculation ◽  
Highly Virulent ◽  
Apparently Healthy

Background: Newcastle disease caused by Avian avulavirus type 1 (AAvV-1) is one of the dreadful diseases affecting poultry and other avian species. Wild birds and several domestic birds are recognized as reservoirs of AAvV-1 and probably contribute to the epidemiology of ND in the domesticated poultry. Hence, efforts have been made to understand the virulence and genetic nature of AAvV-1 isolates obtained from apparently healthy Emu birds.Methods: This study details characterization of a velogenic Emu/5 AAvV-1 isolate obtained from an asymptomatic emu flock. Full- length fusion gene was amplified and subsequent phylogenetic analysis was performed. Experimental inoculation of 3-week old chicken with the isolate resulted in virulent ND. Expression of cytokine mRNA levels in spleen of infected chicken at different time points correlated well with the clinical picture, gross and histopathological lesions.Result: To our knowledge this is the first evidence for the role of apparently healthy emu bird acting as a reservoir of velogenic AAvV-1 of subgenotype XIII 2.2 which proved to be highly virulent to chicken. This study further highlights the role of reservoir birds in AAvV-1 transmission and the need for adopting most realistic strategies in counteracting the disease.

scholarly journals Foxa2 and MafA regulate islet-specific glucose-6-phosphatase catalytic subunit-related protein gene expression

2008 ◽  
Vol 41 (5) ◽  
pp. 315-328 ◽  
Author(s):  
Cyrus C Martin ◽  
Brian P Flemming ◽  
Yingda Wang ◽  
James K Oeser ◽  
Richard M O'Brien
Keyword(s):  
Gene Expression ◽  
Type 1 Diabetes ◽  
Transcription Factors ◽  
Binding Sites ◽  
Fusion Gene ◽  
Fasting Blood Glucose ◽  
Catalytic Subunit ◽  
Related Protein ◽  
Min6 Cells

Islet-specific glucose-6-phosphatase catalytic subunit-related protein (IGRP/G6PC2) is a major autoantigen in both mouse and human type 1 diabetes. IGRP is selectively expressed in islet β cells and polymorphisms in the IGRP gene have recently been associated with variations in fasting blood glucose levels and cardiovascular-associated mortality in humans. Chromatin immunoprecipitation (ChIP) assays have shown that the IGRP promoter binds the islet-enriched transcription factors Pax-6 and BETA2. We show here, again using ChIP assays, that the IGRP promoter also binds the islet-enriched transcription factors MafA and Foxa2. Single binding sites for these factors were identified in the proximal IGRP promoter, mutation of which resulted in decreased IGRP fusion gene expression in βTC-3, Hamster insulinoma tumor (HIT), and Min6 cells. ChiP assays have shown that the islet-enriched transcription factor Pdx-1 also binds the IGRP promoter, but mutational analysis of four Pdx-1 binding sites in the proximal IGRP promoter revealed surprisingly little effect of Pdx-1 binding on IGRP fusion gene expression in βTC-3 cells. In contrast, in both HIT and Min6 cells mutation of these four Pdx-1 binding sites resulted in a ∼50% reduction in fusion gene expression. These data suggest that the same group of islet-enriched transcription factors, namely Pdx-1, Pax-6, MafA, BETA2, and Foxa2, directly or indirectly regulate expression of the two major autoantigens in type 1 diabetes.

scholarly journals Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene.

1991 ◽  
Vol 11 (6) ◽  
pp. 3374-3378 ◽  
Author(s):  
S D Lupton ◽  
L L Brunton ◽  
V A Kalberg ◽  
R W Overell
Keyword(s):  
Thymidine Kinase ◽  
Negative Selection ◽  
Fusion Gene ◽  
Hygromycin B ◽  
Thymidine Kinase Gene ◽  
Kinase Gene ◽  
Hygromycin Phosphotransferase ◽  
Simplex Virus ◽  
Genetic Entity

The hygromycin phosphotransferase gene was fused in-frame with the herpes simplex virus type 1 thymidine kinase gene. The resulting fusion gene (termed HyTK) confers hygromycin B resistance for dominant positive selection and ganciclovir sensitivity for negative selection and provides a means by which these selectable phenotypes may be expressed and regulated as a single genetic entity.

A novel telomerase-T antigen fusion gene capable of immortalizing human type-1 astrocytes

2006 ◽  
Vol 198 (2) ◽  
pp. 587
Author(s):  
J.F. Sanchez ◽  
C.J. Schoen ◽  
L. Shen ◽  
C.T. Lee ◽  
J. Chen ◽  
...  
Keyword(s):  
Fusion Gene ◽  
T Antigen ◽  
Human Type

scholarly journals In Vitro and In Vivo Characterization of a Pigeon Paramyxovirus Type 1 Isolated from Domestic Pigeons in Victoria, Australia 2011

Viruses ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 429
Author(s):  
Songhua Shan ◽  
Kerri Bruce ◽  
Vittoria Stevens ◽  
Frank Wong ◽  
Jianning Wang ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Fusion Gene ◽  
Chicken Embryo Fibroblast ◽  
Haemagglutination Inhibition ◽  
Clinical Signs ◽  
First Time ◽  
Domestic Pigeons

Significant mortalities of racing pigeons occurred in Australia in late 2011 associated with a pigeon paramyxovirus serotype 1 (PPMV-1) infection. The causative agent, designated APMV-1/pigeon/Australia/3/2011 (P/Aus/3/11), was isolated from diagnostic specimens in specific pathogen free (SPF) embryonated eggs and was identified by a Newcastle Disease virus (NDV)-specific RT-PCR and haemagglutination inhibition (HI) test using reference polyclonal antiserum specific for NDV. The P/Aus/3/11 strain was further classified as PPMV-1 using the HI test and monoclonal antibody 617/161 by HI and phylogenetic analysis of the fusion gene sequence. The isolate P/Aus/3/11 had a slow haemagglutin-elution rate and was inactivated within 45 min at 56 °C. Cross HI tests generated an R value of 0.25, indicating a significant antigenic difference between P/Aus/3/11 and NDV V4 isolates. The mean death time (MDT) of SPF eggs infected with the P/Aus/3/11 isolate was 89.2 hr, characteristic of a mesogenic pathotype, consistent with other PPMV-1 strains. The plaque size of the P/Aus/3/11 isolate on chicken embryo fibroblast (CEF) cells was smaller than those of mesogenic and velogenic NDV reference strains, indicating a lower virulence phenotype in vitro and challenge of six-week-old SPF chickens did not induce clinical signs. However, sequence analysis of the fusion protein cleavage site demonstrated an 112RRQKRF117 motif, which is typical of a velogenic NDV pathotype. Phylogenetic analysis indicated that the P/Aus/3/11 isolate belongs to a distinct subgenotype within class II genotype VI of avian paramyxovirus type 1. This is the first time this genotype has been detected in Australia causing disease in domestic pigeons and is the first time since 2002 that an NDV with potential for virulence has been detected in Australia.

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