scholarly journals ADCC Assay

2020 ◽  
Author(s):  
Keyword(s):  
Adcc Assay

Miniaturized Whole Blood ADCC Assay of Depressed Effector Activity in Cancer Patients1

2008 ◽  
Vol 15 (3) ◽  
pp. 225-230 ◽  
Author(s):  
Mutsuo Sasaki ◽  
Beverly Looman ◽  
Paul I. Terasaki
Keyword(s):  
Whole Blood ◽  
Adcc Assay ◽  
Effector Activity

PREDICTION OF THE SEVERITY OF RHESUS HAEMOLYTIC DISEASE OF THE NEWBORN BY AN ADCC ASSAY

The Lancet ◽  
1981 ◽  
Vol 318 (8238) ◽  
pp. 142-143 ◽  
Author(s):  
S.J. Urbaniak ◽  
M. Ayoub Greiss ◽  
R.J. Crawford ◽  
M.C.J. Fergusson
Keyword(s):  
Haemolytic Disease ◽  
Adcc Assay

Engineering therapeutic antibodies for improved function

2002 ◽  
Vol 30 (4) ◽  
pp. 487-490 ◽  
Author(s):  
L. G. Presta ◽  
R. L. Shields ◽  
A. K. Namenuk ◽  
K. Hong ◽  
Y. G. Meng
Keyword(s):  
Binding Sites ◽  
Mononuclear Cells ◽  
Cellular Cytotoxicity ◽  
Antibody Dependent Cellular Cytotoxicity ◽  
Fcγ Receptor ◽  
Peripheral Blood Mononuclear ◽  
Adcc Assay ◽  
Human Igg1 ◽  
Improved Function

The binding sites on human IgG1 for human Fcγ receptor (FcγR) I, FcγRIIa, FcγRIIb, FcγRIIIa and neonatal FcR have been mapped. A common set of IgG1 residues is involved in binding to all FcγRs, while FcγRII and FcγRIII utilize distinct sites outside this common set. In addition to residues which abrogated binding to the FcγR, several positions were found which improved binding only to specific FcγRs or simultaneously improved binding to one type of FcγR and reduced binding to another type. Selected IgG1 variants with improved binding to FcγRIIIa were then tested in an in vitro antibody-dependent cellular cytotoxicity (ADCC) assay and showed an enhancement in ADCC when either peripheral blood mononuclear cells or natural killer cells were used.

Prediction of the Outcome of Rhesus Haemolytic Disease of the Newborn: Additional Information Using an ADCC Assay

Vox Sanguinis ◽  
1984 ◽  
Vol 46 (5) ◽  
pp. 323-329 ◽  
Author(s):  
S.J. Urbaniak ◽  
M. Ayoub Greiss ◽  
R.J. Crawford ◽  
M.J.C. Fergusson
Keyword(s):  
Haemolytic Disease ◽  
Additional Information ◽  
Adcc Assay

scholarly journals Comparison of antibody-dependent cellular cytotoxicity and complement-dependent antibody lysis of herpes simplex virus-infected cells as methods of detecting antiviral antibodies in human sera

1977 ◽  
Vol 5 (6) ◽  
pp. 551-558
Author(s):  
T Subramanian ◽  
W E Rawls
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Cellular Cytotoxicity ◽  
Antibody Dependent Cellular Cytotoxicity ◽  
Effector Cells ◽  
Herpes Simplex Viruses ◽  
Adcc Assay ◽  
Human Sera ◽  
Infected Cells ◽  
Simplex Virus

An antibody-dependent cellular cytotoxicity (ADCC) assay was used to detect antibodies to the herpes simplex viruses in humans sera. The assay utilized the release of 51Cr from BHK-21 cells infected with the viruses, hamster peritoneal exudate cells as effector cells, and antiviral antibodies in human sera. The technique was found to be far more sensitive than complement-dependent antibody lysis of infected cells and virus neutralization. The ADCC assay was useful in detecting antibodies in sera that had low titers or no antibodies detectable by other methods. In a sample of 100 sera from university students, 40 were positive by complement-dependent lysis whereas 73 were positive by ADCC. Of 400 sera from women with cervical cancer, 17 did not have detectable antibodies by microneutralization or complement-dependent lysis; however, all sera were positive by ADCC, suggesting that all of the women had been infected in the past with one or both types of herpes simplex virus.

scholarly journals A novel Ebola virus antibody-dependent cell-mediated cytotoxicity (Ebola ADCC) assay

2018 ◽  
Vol 460 ◽  
pp. 10-16 ◽  
Author(s):  
Karnail Singh ◽  
Bishal Marasini ◽  
Xuemin Chen ◽  
Paul Spearman
Keyword(s):  
Ebola Virus ◽  
Virus Antibody ◽  
Adcc Assay ◽  
Dependent Cell

Development of a simple new flow cytometric antibody-dependent cellular cytotoxicity (ADCC) assay with excellent sensitivity

2019 ◽  
Vol 464 ◽  
pp. 74-86 ◽  
Author(s):  
Miho Tanaka ◽  
Akiko Ishige ◽  
Masami Yaguchi ◽  
Takehisa Matsumoto ◽  
Mikako Shirouzu ◽  
...  
Keyword(s):  
Cellular Cytotoxicity ◽  
Antibody Dependent Cellular Cytotoxicity ◽  
Flow Cytometric ◽  
Adcc Assay

scholarly journals Env-Specific IgA from Viremic HIV-Infected Subjects Compromises Antibody-Dependent Cellular Cytotoxicity

2015 ◽  
Vol 90 (2) ◽  
pp. 670-681 ◽  
Author(s):  
María Julia Ruiz ◽  
Yanina Ghiglione ◽  
Juliana Falivene ◽  
Natalia Laufer ◽  
María Pía Holgado ◽  
...  
Keyword(s):  
Hiv Infection ◽  
Disease Progression ◽  
Acute Infection ◽  
Protective Role ◽  
Cellular Cytotoxicity ◽  
Antibody Dependent Cellular Cytotoxicity ◽  
Elite Controllers ◽  
Before And After ◽  
Adcc Assay ◽  
Underlying Mechanisms

ABSTRACTElucidating the factors that modulate HIV-specific antibody-dependent cellular cytotoxicity (ADCC) will help in understanding its role in HIV immunity. The aim of this study was to determine whether IgA could modify the magnitude of ADCC in HIV infection, abrogating its protective role. Plasma samples from 20 HIV-positive (HIV+) subjects enrolled during primary HIV infection (PHI), 10 chronically infected subjects (chronic), and 7 elite controllers (EC) were used. ADCC was determined by using a fluorometric ADCC assay, before and after removal of plasma IgA. Data were analyzed by using nonparametric statistics. ADCC was documented in 80% of PHI enrollment samples and in 100% of PHI 12-month, chronic, and EC samples; it peaked after acute infection, reached a plateau in chronic infection, and decreased after initiation of antiretroviral treatment (ART). Significant associations between ADCC and disease progression were found only after removal of plasma IgA from 12-month PHI samples: the magnitude of ADCC not only increased after IgA removal but also correlated with CD4+T-cell preservation. This work provides evidence that gp120-specific IgA was capable of modifying ADCC responses during natural HIV infection for the first time and adds to similar evidence provided in other settings. Furthermore, it underscores the complexity of the ADCC phenomenon and will help in an understanding of its underlying mechanisms.IMPORTANCEAlthough the induction of ADCC-mediating antibodies in HIV-infected subjects has been extensively documented, the association of these antibodies with protection from disease progression is poorly understood. Here, we demonstrate that plasma IgA is a factor capable of modifying the magnitude of IgG-mediated ADCC in HIV infection, mitigating its beneficial effect. These results help in understanding why previous studies failed to demonstrate correlations between ADCC and disease progression, and they also contribute to the notion that an HIV vaccine should stimulate the production of ADCC-mediating IgG antibodies but not IgA.

scholarly journals Development and Assessment of a Pooled Serum as Candidate Standard to Measure Influenza A Virus Group 1 Hemagglutinin Stalk-Reactive Antibodies

Vaccines ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 666
Author(s):  
Juan Manuel Carreño ◽  
Jacqueline U. McDonald ◽  
Tara Hurst ◽  
Peter Rigsby ◽  
Eleanor Atkinson ◽  
...  
Keyword(s):  
Influenza A ◽  
Collaborative Study ◽  
Neutralization Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Neutralization Titer ◽  
Adcc Assay ◽  
Dependent Cell ◽  
Stalk Domain ◽  
Group 1

The stalk domain of the hemagglutinin has been identified as a target for induction of protective antibody responses due to its high degree of conservation among numerous influenza subtypes and strains. However, current assays to measure stalk-based immunity are not standardized. Hence, harmonization of assay readouts would help to compare experiments conducted in different laboratories and increase confidence in results. Here, serum samples from healthy individuals (n = 110) were screened using a chimeric cH6/1 hemagglutinin enzyme-linked immunosorbent assay (ELISA) that measures stalk-reactive antibodies. We identified samples with moderate to high IgG anti-stalk antibody levels. Likewise, screening of the samples using the mini-hemagglutinin (HA) headless construct #4900 and analysis of the correlation between the two assays confirmed the presence and specificity of anti-stalk antibodies. Additionally, samples were characterized by a cH6/1N5 virus-based neutralization assay, an antibody-dependent cell-mediated cytotoxicity (ADCC) assay, and competition ELISAs, using the stalk-reactive monoclonal antibodies KB2 (mouse) and CR9114 (human). A “pooled serum” (PS) consisting of a mixture of selected serum samples was generated. The PS exhibited high levels of stalk-reactive antibodies, had a cH6/1N5-based neutralization titer of 320, and contained high levels of stalk-specific antibodies with ADCC activity. The PS, along with blinded samples of varying anti-stalk antibody titers, was distributed to multiple collaborators worldwide in a pilot collaborative study. The samples were subjected to different assays available in the different laboratories, to measure either binding or functional properties of the stalk-reactive antibodies contained in the serum. Results from binding and neutralization assays were analyzed to determine whether use of the PS as a standard could lead to better agreement between laboratories. The work presented here points the way towards the development of a serum standard for antibodies to the HA stalk domain of phylogenetic group 1.

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