scholarly journals Cullin-4B

2020 ◽  
Author(s):  
Keyword(s):  
Cullin 4B

Cullin 4B is a novel prognostic marker that correlates with colon cancer progression and pathogenesis

2013 ◽  
Vol 30 (2) ◽  
Author(s):  
Tao Jiang ◽  
Hua-mei Tang ◽  
Ze-hua Wu ◽  
Jian Chen ◽  
Su Lu ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Prognostic Marker ◽  
Cancer Progression ◽  
Colon Cancer Progression ◽  
Cullin 4B

scholarly journals Cullin4 E3 Ubiquitin Ligases Regulate Male Gonocyte Migration, Proliferation and Blood-Testis Barrier Homeostasis

Cells ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2732
Author(s):  
Yan Yin ◽  
Liming Zhu ◽  
Qiufang Li ◽  
Pengbo Zhou ◽  
Liang Ma
Keyword(s):  
Germ Cell ◽  
Germ Cells ◽  
Cell Lineage ◽  
Functional Redundancy ◽  
Cell Phenotype ◽  
Double Knockout ◽  
Embryonic Germ Cells ◽  
Cullin 4A ◽  
Cullin 4B ◽  
Blood Testis Barrier

Ubiquitination, an essential posttranslational modification, plays fundamental roles during mammalian spermatogenesis. We previously reported the requirement of two Cullin 4 ubiquitin ligase family genes, Cullin 4a (Cul4a) and Cullin 4b (Cul4b), in murine spermatogenesis. Both genes are required for male fertility despite their distinct functions in different cell populations. Cul4a is required in primary spermatocytes to promote meiosis while Cul4b is required in secondary spermatocytes for spermiogenesis. As the two genes encode proteins that are highly homologous and have overlapping expression in embryonic germ cells, they may compensate for each other during germ cell development. In the present study, we directly address the potential functional redundancy of these two proteins by deleting both Cul4 genes, specifically, in the germ cell lineage during embryonic development, using the germ-cell specific Vasa-Cre line. Conditional double-knockout (dKO) males showed delayed homing and impaired proliferation of gonocytes, and a complete loss of germ cells before the end of the first wave of spermatogenesis. The dKO male germ cell phenotype is much more severe than those observed in either single KO mutant, demonstrating the functional redundancy between the two CUL4 proteins. The dKO mutant also exhibited atypical tight junction structures, suggesting the potential involvement of CUL4 proteins in spermatogonial stem cell (SSC) niche formation and blood–testis-barrier (BTB) maintenance. We also show that deleting Cul4b in both germ and Sertoli cells is sufficient to recapitulate part of this phenotype, causing spermatogenesis defects and drastically reduced number of mature sperms, accompanied by defective tight junctions in the mutant testes. These results indicate the involvement of CUL4B in maintaining BTB integrity.

Proteomic analysis of the cullin 4B interactome using proximity-dependent biotinylation in living cells

PROTEOMICS ◽  
2017 ◽  
Vol 17 (8) ◽  
pp. 1600163 ◽  
Author(s):  
Hailong Zhang ◽  
Shupeng Li ◽  
Pingting Liu ◽  
Frankie H. F. Lee ◽  
Albert H. C. Wong ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Living Cells ◽  
Cullin 4B

Cullin 4b Complex Targets IRGM1 to Regulate Intestinal Stem Cell Stemness and Niche

2021 ◽  
Author(s):  
Yujia Fan ◽  
Xiaohan Huo ◽  
Beibei Guo ◽  
Xiaohui Zhang ◽  
Yang Yang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Intestinal Stem Cell ◽  
Cullin 4B

scholarly journals The Cullin 4B–Based UV-Damaged DNA-Binding Protein Ligase Binds to UV-Damaged Chromatin and Ubiquitinates Histone H2A

2008 ◽  
Vol 68 (13) ◽  
pp. 5014-5022 ◽  
Author(s):  
Jennifer Guerrero-Santoro ◽  
Maria G. Kapetanaki ◽  
Ching L. Hsieh ◽  
Ilya Gorbachinsky ◽  
Arthur S. Levine ◽  
...  
Keyword(s):  
Dna Binding ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Histone H2a ◽  
Cullin 4B ◽  
Damaged Dna

scholarly journals Cullin-4B E3 ubiquitin ligase mediates Apaf-1 ubiquitination to regulate caspase-9 activity

PLoS ONE ◽  
2019 ◽  
Vol 14 (7) ◽  
pp. e0219782 ◽  
Author(s):  
Eri Ohta ◽  
Masanori Itoh ◽  
Masashi Ueda ◽  
Yoko Hida ◽  
Miao-xing Wang ◽  
...  
Keyword(s):  
Ubiquitin Ligase ◽  
E3 Ubiquitin Ligase ◽  
Caspase 9 ◽  
Cullin 4B
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